Ezr (NM_019357) Rat Tagged ORF Clone
CAT#: RR212289
- TrueORF®
Ezr (Myc-DDK-tagged ORF) - Rat ezrin (Ezr), (10 ug)
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_019357" in other vectors (3)
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Ezr |
Synonyms | Vil2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR212289 representing NM_019357
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCAAGCCAATCAACGTCCGCGTGACCACCATGGATGCAGAGCTGGAGTTTGCCATCCAGCCAAACA CCACCGGGAAGCAGCTCTTTGATCAGGTGGTAAAGACCATTGGCCTCCGGGAAGTGTGGTACTTCGGCCT CCAGTATGTAGACAATAAAGGATTTCCTACCTGGCTGAAACTTGATAAAAAGGTCTCTGCACAGGAGGTT CGAAAGGAGAACCCTGTCCAGTTCAAATTCCGGGCCAAGTTCTACCCCGAGGATGTGGCTGATGAGCTCA TCCAGGACATCACACAGAAGCTCTTCTTCCTGCAAGTCAAGGAAGGGATCCTCAGCGATGAGATCTACTG CCCCCCAGAGACAGCCGTGCTCCTGGGGTCCTATGCCGTTCAGGCCAAGTTCGGAGATTATAACAAGGAA ATGCACAAGTCTGGGTACCTCAGCTCCGAGAGACTTATCCCTCAGAGAGTCATGGACCAGCACAAGCTCA GCAGGGACCAGTGGGAGGACCGGATCCAGGTGTGGCACGCAGAACACCGAGGGATGCTCAAGGACAGTGC TATGCTCGAATACCTGAAGATTGCCCAGGACCTGGAAATGTATGGGATCAACTATTTCGAGATCAAAAAC AAGAAAGGAACAGATCTTTGGCTTGGAGTGGATGCCCTTGGACTTAACATTTATGAGAAAGATGACAAGT TGACCCCGAAGATCGGCTTCCCTTGGAGTGAAATCAGGAACATCTCTTTCAACGACAAGAAGTTTGTCAT CAAGCCCATCGACAAGAAGGCTCCTGACTTCGTGTTCTACGCTCCCCGCCTGAGAATTAACAAGCGGATT CTGCAGCTCTGCATGGGGAACCACGAGCTGTACATGCGCCGCAGGAAGCCCGACACCATCGAGGTGCAGC AGATGAAGGCCCAGGCTCGGGAGGAGAAGCACCAGAAGCAGCTGGAGCGACAACAGTTGGAAACCGAGAA GAAGAGGAGAGAAACTGTAGAGAGAGAAAAGGAACAGATGCTCCGGGAGAAGGAAGAGCTGATGCTTCGG CTGCAGGACTTTGAGCAGAAGACCAAGAGGGCAGAGAAAGAGCTCTCCGAGCAGATTGAGAAGGCCCTCC AGCTGGAGGAAGAGAGGAGGCGAGCCCAGGAGGAGGCTGAACGTCTGGAGGCCGACCGCATGGCCGCCCT GCGGGCCAAGGAAGAACTGGAGAGACAGGCACAGGATCAGATAAAGAGCCAGGAGCAGCTGGCTGCAGAG CTGGCAGAGTATACTGCCAAGATTGCCCTGCTGGAGGAGGCACGGAGGCGCAAGGAGGACGAGGTGGAGG AGTGGCAGCACCGGGCTAAAGAGGCCCAGGACGACCTGGTGAAGACCAAAGAGGAGCTGCACCTGGTGAT GACGGCTCCCCCGCCCCCACCCCCGCCCGTGTACGAGCCCGTGAATTACCACGTGCAGGAGGGGCTGCAG GATGAGGGAGCAGAGCCCATGGGCTACAGTGCCGAGCTCTCCAGCGAGGGCATCCTGGACGACCGTAACG AGGAGAAGCGGATCACCGAGGCAGAGAAGAACGAGCGCGTGCAGCGGCAGCTGCTGACTCTGAGCAATGA GTTGTCCCAGGCCCGGGATGAGAATAAGAGGACCCACAATGACATCATCCACAATGAGAACATGCGGCAA GGCCGGGACAAGTATAAGACGCTGCGGCAGATCAGGCAGGGCAACACCAAGCAGCGCATCGACGAGTTTG AGGCCATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR212289 representing NM_019357
Red=Cloning site Green=Tags(s) MPKPINVRVTTMDAELEFAIQPNTTGKQLFDQVVKTIGLREVWYFGLQYVDNKGFPTWLKLDKKVSAQEV RKENPVQFKFRAKFYPEDVADELIQDITQKLFFLQVKEGILSDEIYCPPETAVLLGSYAVQAKFGDYNKE MHKSGYLSSERLIPQRVMDQHKLSRDQWEDRIQVWHAEHRGMLKDSAMLEYLKIAQDLEMYGINYFEIKN KKGTDLWLGVDALGLNIYEKDDKLTPKIGFPWSEIRNISFNDKKFVIKPIDKKAPDFVFYAPRLRINKRI LQLCMGNHELYMRRRKPDTIEVQQMKAQAREEKHQKQLERQQLETEKKRRETVEREKEQMLREKEELMLR LQDFEQKTKRAEKELSEQIEKALQLEEERRRAQEEAERLEADRMAALRAKEELERQAQDQIKSQEQLAAE LAEYTAKIALLEEARRRKEDEVEEWQHRAKEAQDDLVKTKEELHLVMTAPPPPPPPVYEPVNYHVQEGLQ DEGAEPMGYSAELSSEGILDDRNEEKRITEAEKNERVQRQLLTLSNELSQARDENKRTHNDIIHNENMRQ GRDKYKTLRQIRQGNTKQRIDEFEAM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_019357 |
ORF Size | 1758 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_019357.1, NP_062230.1 |
RefSeq Size | 2973 bp |
RefSeq ORF | 1761 bp |
Locus ID | 54319 |
UniProt ID | P31977 |
Cytogenetics | 1q11 |
MW | 69.4 kDa |
Gene Summary | connects the microvillar cytoskeleton to the plasma membrane [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN212289 | Ezr (untagged ORF) - Rat ezrin (Ezr), (10 ug) |
USD 601.00 |
|
RR212289L3 | Lenti ORF clone of Ezr (Myc-DDK-tagged ORF) - Rat ezrin (Ezr), (10 ug) |
USD 900.00 |
|
RR212289L4 | Lenti ORF clone of Ezr (mGFP-tagged ORF) - Rat ezrin (Ezr), (10 ug) |
USD 900.00 |
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