Prg4 (NM_001105962) Rat Tagged ORF Clone
CAT#: RR212205
- TrueORF®
Prg4 (Myc-DDK-tagged ORF) - Rat proteoglycan 4, (megakaryocyte stimulating factor, articular superficial zone protein, camptodactyly, arthropathy, coxa vara, pericarditis syndrome) (Prg4, (10 ug)
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_001105962" in other vectors (3)
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Prg4 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR212205 representing NM_001105962
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGTGGAAAATACTTCCCATCTGCTTGTCGATGCTGCTTCCTGTTTTCCTGATTCAACAAGTTTCAT CTCAAGATATATCAAGTTGTGCAGGGAGATGTGGGGAAGGGTATTCTAGAGACGCCACCTGCAACTGTGA TTATAACTGCCAACACTACATGGAGTGCTGTCCTGATTTCAAGAGAGTCTGCACTACAGAGCTCTCCTGC AAAGGACGCTGCTTCGAGTCCTTCGCACGAGGGAGGGAGTGTGACTGTGACTCCCAGTGTAAACAGTACG GCAAATGCTGTGCAGACTACAACAGCTTCTGCGAAGAAGTGCATAATCCCACATCACCACCTCCAAAGAC TGCACCCCCGCCTCCAGGAGCGTCTCGCACCATCAAATCAACCACCAAACGTTCACCCAAATCACCAAAA AAGAAGACTAAGAAAGTTGTAGAGTCTGAGGAAATAACAGAAGAACATTCTGTTTCTGAAAATCAAGAGT CCTCCTCCTCCTCCTCTTCCTCCTCTTCAACTATTCGGAAAATCAAGTCTTCCAAAAATTCAGCTAATAG AGAATTACAGAAGAACCCAAAAGTAAAAGATAACAAGAAAAACACCCCGAAAAAGAAACCTAATCCAGAA CCACCAGTCGTGGATGAAGCTGGAAGTGGTCTGGACAATGGTGAGCCCAAGCTTACCCCACCTCCTGACC CTCCTACTCCACACAACAAAGTCACCGCATCTCCCAAGACTACAGCGGCAAAACCAGTAACTCCTAAACC CAGTCTTGCACCTAATTCTGAGACTTCCAAAGAGGCATCTTCAACTTCCAATAAAGAGACAACAGTTGAA ACAAAGGAGACCACTGAAACAAATAAACAGTCTTCCGCCAGTAAAAAAGAGAAGACTACTTCAGCTAAAG AGACACGGAGTGCAGAGAACACATCTGCTAAAGACGTTGAACCCACATCTACAACTCCCAAGAACTCAGC TCCCACTACCACCAAGAAGCCTGTAACTACAACCAAGGAACCTGTTCCTACCACTACCAAGGGGCCTGAA CCCACCTCCAAGGAGCCAGCTCCCACCACCCCTAAGGAGCCTGAACTCACCATTCCCAAAGAACCAGCTC CCACCACCAAGAAGCCAGCTCCCACCACCCCCAAGGAGCCAGCTCCCACCACCCCTAAGGAGCCAGCTCC CACCACCCCCAAGGAGCCAGCTCCCACCACCAAGAAGCCTGAACCCACCACACCTAAGGAGCCAGCTCCC ACCACCCCCAAGGAGCCAGCTCCCACCACCCCCAAGGAGCCAGCTCCCAATACCCCTAAGGAGCCAGCTC CCACCACCCCCAAGGAGCCAGCTCCCACCACCCCTAAGGAGCCTGAATCCACCACCCCCAAGGAACCAGC TCCCACCACCCCCAAGGAGCCAGCTCCCACCACCCCCAAGGAGCCAGCTCCCACCACCCCCAAGGAGCCT GCTCCCACTACCCCTAAGGAGCCAGCTCCCACCACCCCCAAGGAGCCAGCTCTTACTACCCCTAAGGAGC CAGCTCCCACCCCCAAAGAGCCTGAACCCACCACCCCTAAGGAGCCAGCTCCCACCACCCCTAAGGAGCC AGCTCCCACCACCAAGAAGCCTGAACTCACCATCCCCAAGGAGCCAGTTCCTACCACCCCCAAAGAACCA GCTCCTACCACTCCCAAAGAGCCTGCACCCACCACCACCAAGAAGCCTGAACTCAGCACCCCTAAGGAGC CCACATCTACTAACCCCAAGGAACCTGAACCCACCCTCCCCAAGGAGCCTGAACTCACCCTCCCCGAGGA GCCTGAACCCAGCAACCCCGAGACATCCGATGAATTAATAGCTGAGCTTGCTGAGGCACCCACACCAAAG GCTCTTGAAAATAATCCCAAGGAACCCACTGTACCAACTGAGACTCCTGAGGTAAGCAAGCCTGAAATGA CAACTGCTAAAGAGAAGACAACAGAAAAAGACACAACCCAAACTACAACAACAGTAACCCCTAAGACAAC AACAGAGACAACAATCATACCGGAAGAGACTACTGAACCAAGAGCAAGTACCACCACTCGAATAACGTCT ACAGCTCAAGATACCACATCACCCCAAATGACTACTCTGAAAGCTACAACTCTTGCAGCCAAAGTAACTG CTCCAACAGAAGAGATTCAGAGCAAACCAGAAGAAACAACTTTAACAACTCCTGCATTGGAAGGTTCTGA TGCTTCTAAGACAACCCTAAAACCTCAGAAACCAACCAAAGCGCCCAAGAAGCCCGCCTCTACCAAAAAG CCAACCAAAGCGCCCAAGAAGCCCACCTCTACCAAAAAGCCAAAGACACCAAAAGGGAGAAAACCAAAAA CCACACCATCTCCTCTTAAGACTTCAACAATGTCTGAACAGAATACCACTCCTCTAGAAGTCATGCTGCC AACCACCACCGTCCCTAAACAAACTCCAAACTCTGAAACAGCTGAAGTGAATCCAGCTCATGAAGATGCA GATGGAGGTGAAGGAGAAAAACCTCTGATTCCCAGGCCCCCTGTGCTATCCCCCACTGCTGTTCCAGGAA CCGATCTCTTGGTTGAGAGACTCAATCCAGGCATTAACATCCATCCCATGTTTTCAGATGAGACCAATAT ATGCAATGGTAAGCCAGTGGATGGACTGACCACTCTGCGCAACGGGACGTTAGTTGCATTTCGAGGTCAT TATTTCTGGATGCTGAACCCATTCAGACCACCATCTCCACCACGCAGAATCACTGAAGTCTGGGGTATTC CCTCTCCCATTGACACGGTTTTTACTAGATGTAACTGTGAAGGAAAAACTTTCTTCTTTAAGGATTCTCA GTACTGGCGCTTTACCAACGATGTAATGGATGCTGGGTATCCTAAACTAATTGTCAAAGGCTTTGGAGGA CTAACAGGGAAGATAGTGGCTGCTCTTTCAATAGCCAAGTACAAGGACAGACCTGAATCTGTGTACTTCT TCAAGAGAGGTGGCAACATCCAGCAGTACACTTACAAACAGGAGCCCGTGAGGAAGTGCACAGGGAGGCA GCCTGCCATCAACTACTCAGTGTATGGGCAGGCAGCACAGGTCAGGAGGCGCCGCTTTGAGCGTGCTGTT GGACCTTTCCAGACACACACCTTCAGGATCCATTACTCGGTGCCCATCAAAGCCTCTTATCAAGACAAAG GCTTCCTCCACAATGAAGTCAAAGTGAGCACACTGTGGAGAGGGTTCCCAAATGTCGTTACTTCCGCGAT AACACTGCCCAACATTAGAAAACCTGACGGCTATGATTACTACGCCTTTTCCAAAGATCAATACTATAAC ATCGATGTGCCTACTAGAACAGCAAGAGCAATCACCACGCGCTCAGGGCAGACCCTCTCCAGAGTCTGGT ACAACTGTCCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR212205 representing NM_001105962
Red=Cloning site Green=Tags(s) MGWKILPICLSMLLPVFLIQQVSSQDISSCAGRCGEGYSRDATCNCDYNCQHYMECCPDFKRVCTTELSC KGRCFESFARGRECDCDSQCKQYGKCCADYNSFCEEVHNPTSPPPKTAPPPPGASRTIKSTTKRSPKSPK KKTKKVVESEEITEEHSVSENQESSSSSSSSSSTIRKIKSSKNSANRELQKNPKVKDNKKNTPKKKPNPE PPVVDEAGSGLDNGEPKLTPPPDPPTPHNKVTASPKTTAAKPVTPKPSLAPNSETSKEASSTSNKETTVE TKETTETNKQSSASKKEKTTSAKETRSAENTSAKDVEPTSTTPKNSAPTTTKKPVTTTKEPVPTTTKGPE PTSKEPAPTTPKEPELTIPKEPAPTTKKPAPTTPKEPAPTTPKEPAPTTPKEPAPTTKKPEPTTPKEPAP TTPKEPAPTTPKEPAPNTPKEPAPTTPKEPAPTTPKEPESTTPKEPAPTTPKEPAPTTPKEPAPTTPKEP APTTPKEPAPTTPKEPALTTPKEPAPTPKEPEPTTPKEPAPTTPKEPAPTTKKPELTIPKEPVPTTPKEP APTTPKEPAPTTTKKPELSTPKEPTSTNPKEPEPTLPKEPELTLPEEPEPSNPETSDELIAELAEAPTPK ALENNPKEPTVPTETPEVSKPEMTTAKEKTTEKDTTQTTTTVTPKTTTETTIIPEETTEPRASTTTRITS TAQDTTSPQMTTLKATTLAAKVTAPTEEIQSKPEETTLTTPALEGSDASKTTLKPQKPTKAPKKPASTKK PTKAPKKPTSTKKPKTPKGRKPKTTPSPLKTSTMSEQNTTPLEVMLPTTTVPKQTPNSETAEVNPAHEDA DGGEGEKPLIPRPPVLSPTAVPGTDLLVERLNPGINIHPMFSDETNICNGKPVDGLTTLRNGTLVAFRGH YFWMLNPFRPPSPPRRITEVWGIPSPIDTVFTRCNCEGKTFFFKDSQYWRFTNDVMDAGYPKLIVKGFGG LTGKIVAALSIAKYKDRPESVYFFKRGGNIQQYTYKQEPVRKCTGRQPAINYSVYGQAAQVRRRRFERAV GPFQTHTFRIHYSVPIKASYQDKGFLHNEVKVSTLWRGFPNVVTSAITLPNIRKPDGYDYYAFSKDQYYN IDVPTRTARAITTRSGQTLSRVWYNCP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001105962 |
ORF Size | 3441 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001105962.3, NP_001099432.3 |
RefSeq Size | 3849 bp |
RefSeq ORF | 3444 bp |
Locus ID | 289104 |
Cytogenetics | 13q21 |
MW | 124.8 kDa |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN212205 | Prg4 (untagged ORF) - Rat proteoglycan 4, (megakaryocyte stimulating factor, articular superficial zone protein, camptodactyly, arthropathy, coxa vara, pericarditis syndrome) (Prg4), (10 ug) |
USD 1,099.00 |
|
RR212205L3 | Lenti ORF clone of Prg4 (Myc-DDK-tagged ORF) - Rat proteoglycan 4, (megakaryocyte stimulating factor, articular superficial zone protein, camptodactyly, arthropathy, coxa vara, pericarditis syndrome) (Prg4, (10 ug) |
USD 1,398.00 |
|
RR212205L4 | Lenti ORF clone of Prg4 (mGFP-tagged ORF) - Rat proteoglycan 4, (megakaryocyte stimulating factor, articular superficial zone protein, camptodactyly, arthropathy, coxa vara, pericarditis syndrome) (Prg4, (10 ug) |
USD 1,398.00 |
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