Prg4 (NM_001105962) Rat Tagged ORF Clone

CAT#: RR212205

Prg4 (Myc-DDK-tagged ORF) - Rat proteoglycan 4, (megakaryocyte stimulating factor, articular superficial zone protein, camptodactyly, arthropathy, coxa vara, pericarditis syndrome) (Prg4, (10 ug)

Product Images

Circular map for RR212205

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Prg4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR212205 representing NM_001105962
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGTGGAAAATACTTCCCATCTGCTTGTCGATGCTGCTTCCTGTTTTCCTGATTCAACAAGTTTCAT
  CTCAAGATATATCAAGTTGTGCAGGGAGATGTGGGGAAGGGTATTCTAGAGACGCCACCTGCAACTGTGA
  TTATAACTGCCAACACTACATGGAGTGCTGTCCTGATTTCAAGAGAGTCTGCACTACAGAGCTCTCCTGC
  AAAGGACGCTGCTTCGAGTCCTTCGCACGAGGGAGGGAGTGTGACTGTGACTCCCAGTGTAAACAGTACG
  GCAAATGCTGTGCAGACTACAACAGCTTCTGCGAAGAAGTGCATAATCCCACATCACCACCTCCAAAGAC
  TGCACCCCCGCCTCCAGGAGCGTCTCGCACCATCAAATCAACCACCAAACGTTCACCCAAATCACCAAAA
  AAGAAGACTAAGAAAGTTGTAGAGTCTGAGGAAATAACAGAAGAACATTCTGTTTCTGAAAATCAAGAGT
  CCTCCTCCTCCTCCTCTTCCTCCTCTTCAACTATTCGGAAAATCAAGTCTTCCAAAAATTCAGCTAATAG
  AGAATTACAGAAGAACCCAAAAGTAAAAGATAACAAGAAAAACACCCCGAAAAAGAAACCTAATCCAGAA
  CCACCAGTCGTGGATGAAGCTGGAAGTGGTCTGGACAATGGTGAGCCCAAGCTTACCCCACCTCCTGACC
  CTCCTACTCCACACAACAAAGTCACCGCATCTCCCAAGACTACAGCGGCAAAACCAGTAACTCCTAAACC
  CAGTCTTGCACCTAATTCTGAGACTTCCAAAGAGGCATCTTCAACTTCCAATAAAGAGACAACAGTTGAA
  ACAAAGGAGACCACTGAAACAAATAAACAGTCTTCCGCCAGTAAAAAAGAGAAGACTACTTCAGCTAAAG
  AGACACGGAGTGCAGAGAACACATCTGCTAAAGACGTTGAACCCACATCTACAACTCCCAAGAACTCAGC
  TCCCACTACCACCAAGAAGCCTGTAACTACAACCAAGGAACCTGTTCCTACCACTACCAAGGGGCCTGAA
  CCCACCTCCAAGGAGCCAGCTCCCACCACCCCTAAGGAGCCTGAACTCACCATTCCCAAAGAACCAGCTC
  CCACCACCAAGAAGCCAGCTCCCACCACCCCCAAGGAGCCAGCTCCCACCACCCCTAAGGAGCCAGCTCC
  CACCACCCCCAAGGAGCCAGCTCCCACCACCAAGAAGCCTGAACCCACCACACCTAAGGAGCCAGCTCCC
  ACCACCCCCAAGGAGCCAGCTCCCACCACCCCCAAGGAGCCAGCTCCCAATACCCCTAAGGAGCCAGCTC
  CCACCACCCCCAAGGAGCCAGCTCCCACCACCCCTAAGGAGCCTGAATCCACCACCCCCAAGGAACCAGC
  TCCCACCACCCCCAAGGAGCCAGCTCCCACCACCCCCAAGGAGCCAGCTCCCACCACCCCCAAGGAGCCT
  GCTCCCACTACCCCTAAGGAGCCAGCTCCCACCACCCCCAAGGAGCCAGCTCTTACTACCCCTAAGGAGC
  CAGCTCCCACCCCCAAAGAGCCTGAACCCACCACCCCTAAGGAGCCAGCTCCCACCACCCCTAAGGAGCC
  AGCTCCCACCACCAAGAAGCCTGAACTCACCATCCCCAAGGAGCCAGTTCCTACCACCCCCAAAGAACCA
  GCTCCTACCACTCCCAAAGAGCCTGCACCCACCACCACCAAGAAGCCTGAACTCAGCACCCCTAAGGAGC
  CCACATCTACTAACCCCAAGGAACCTGAACCCACCCTCCCCAAGGAGCCTGAACTCACCCTCCCCGAGGA
  GCCTGAACCCAGCAACCCCGAGACATCCGATGAATTAATAGCTGAGCTTGCTGAGGCACCCACACCAAAG
  GCTCTTGAAAATAATCCCAAGGAACCCACTGTACCAACTGAGACTCCTGAGGTAAGCAAGCCTGAAATGA
  CAACTGCTAAAGAGAAGACAACAGAAAAAGACACAACCCAAACTACAACAACAGTAACCCCTAAGACAAC
  AACAGAGACAACAATCATACCGGAAGAGACTACTGAACCAAGAGCAAGTACCACCACTCGAATAACGTCT
  ACAGCTCAAGATACCACATCACCCCAAATGACTACTCTGAAAGCTACAACTCTTGCAGCCAAAGTAACTG
  CTCCAACAGAAGAGATTCAGAGCAAACCAGAAGAAACAACTTTAACAACTCCTGCATTGGAAGGTTCTGA
  TGCTTCTAAGACAACCCTAAAACCTCAGAAACCAACCAAAGCGCCCAAGAAGCCCGCCTCTACCAAAAAG
  CCAACCAAAGCGCCCAAGAAGCCCACCTCTACCAAAAAGCCAAAGACACCAAAAGGGAGAAAACCAAAAA
  CCACACCATCTCCTCTTAAGACTTCAACAATGTCTGAACAGAATACCACTCCTCTAGAAGTCATGCTGCC
  AACCACCACCGTCCCTAAACAAACTCCAAACTCTGAAACAGCTGAAGTGAATCCAGCTCATGAAGATGCA
  GATGGAGGTGAAGGAGAAAAACCTCTGATTCCCAGGCCCCCTGTGCTATCCCCCACTGCTGTTCCAGGAA
  CCGATCTCTTGGTTGAGAGACTCAATCCAGGCATTAACATCCATCCCATGTTTTCAGATGAGACCAATAT
  ATGCAATGGTAAGCCAGTGGATGGACTGACCACTCTGCGCAACGGGACGTTAGTTGCATTTCGAGGTCAT
  TATTTCTGGATGCTGAACCCATTCAGACCACCATCTCCACCACGCAGAATCACTGAAGTCTGGGGTATTC
  CCTCTCCCATTGACACGGTTTTTACTAGATGTAACTGTGAAGGAAAAACTTTCTTCTTTAAGGATTCTCA
  GTACTGGCGCTTTACCAACGATGTAATGGATGCTGGGTATCCTAAACTAATTGTCAAAGGCTTTGGAGGA
  CTAACAGGGAAGATAGTGGCTGCTCTTTCAATAGCCAAGTACAAGGACAGACCTGAATCTGTGTACTTCT
  TCAAGAGAGGTGGCAACATCCAGCAGTACACTTACAAACAGGAGCCCGTGAGGAAGTGCACAGGGAGGCA
  GCCTGCCATCAACTACTCAGTGTATGGGCAGGCAGCACAGGTCAGGAGGCGCCGCTTTGAGCGTGCTGTT
  GGACCTTTCCAGACACACACCTTCAGGATCCATTACTCGGTGCCCATCAAAGCCTCTTATCAAGACAAAG
  GCTTCCTCCACAATGAAGTCAAAGTGAGCACACTGTGGAGAGGGTTCCCAAATGTCGTTACTTCCGCGAT
  AACACTGCCCAACATTAGAAAACCTGACGGCTATGATTACTACGCCTTTTCCAAAGATCAATACTATAAC
  ATCGATGTGCCTACTAGAACAGCAAGAGCAATCACCACGCGCTCAGGGCAGACCCTCTCCAGAGTCTGGT
  ACAACTGTCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR212205 representing NM_001105962
  Red=Cloning site Green=Tags(s)
  
  MGWKILPICLSMLLPVFLIQQVSSQDISSCAGRCGEGYSRDATCNCDYNCQHYMECCPDFKRVCTTELSC
  KGRCFESFARGRECDCDSQCKQYGKCCADYNSFCEEVHNPTSPPPKTAPPPPGASRTIKSTTKRSPKSPK
  KKTKKVVESEEITEEHSVSENQESSSSSSSSSSTIRKIKSSKNSANRELQKNPKVKDNKKNTPKKKPNPE
  PPVVDEAGSGLDNGEPKLTPPPDPPTPHNKVTASPKTTAAKPVTPKPSLAPNSETSKEASSTSNKETTVE
  TKETTETNKQSSASKKEKTTSAKETRSAENTSAKDVEPTSTTPKNSAPTTTKKPVTTTKEPVPTTTKGPE
  PTSKEPAPTTPKEPELTIPKEPAPTTKKPAPTTPKEPAPTTPKEPAPTTPKEPAPTTKKPEPTTPKEPAP
  TTPKEPAPTTPKEPAPNTPKEPAPTTPKEPAPTTPKEPESTTPKEPAPTTPKEPAPTTPKEPAPTTPKEP
  APTTPKEPAPTTPKEPALTTPKEPAPTPKEPEPTTPKEPAPTTPKEPAPTTKKPELTIPKEPVPTTPKEP
  APTTPKEPAPTTTKKPELSTPKEPTSTNPKEPEPTLPKEPELTLPEEPEPSNPETSDELIAELAEAPTPK
  ALENNPKEPTVPTETPEVSKPEMTTAKEKTTEKDTTQTTTTVTPKTTTETTIIPEETTEPRASTTTRITS
  TAQDTTSPQMTTLKATTLAAKVTAPTEEIQSKPEETTLTTPALEGSDASKTTLKPQKPTKAPKKPASTKK
  PTKAPKKPTSTKKPKTPKGRKPKTTPSPLKTSTMSEQNTTPLEVMLPTTTVPKQTPNSETAEVNPAHEDA
  DGGEGEKPLIPRPPVLSPTAVPGTDLLVERLNPGINIHPMFSDETNICNGKPVDGLTTLRNGTLVAFRGH
  YFWMLNPFRPPSPPRRITEVWGIPSPIDTVFTRCNCEGKTFFFKDSQYWRFTNDVMDAGYPKLIVKGFGG
  LTGKIVAALSIAKYKDRPESVYFFKRGGNIQQYTYKQEPVRKCTGRQPAINYSVYGQAAQVRRRRFERAV
  GPFQTHTFRIHYSVPIKASYQDKGFLHNEVKVSTLWRGFPNVVTSAITLPNIRKPDGYDYYAFSKDQYYN
  IDVPTRTARAITTRSGQTLSRVWYNCP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001105962
• ORF Size: 3441 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001105962.3, NP_001099432.3
• RefSeq Size: 3849 bp
• RefSeq ORF: 3444 bp
• Locus ID: 289104
• Cytogenetics: 13q21
• MW: 124.8 kDa