Anks1a (NM_001107613) Rat Tagged ORF Clone

CAT#: RR212066

Anks1a (Myc-DDK-tagged ORF) - Rat ankyrin repeat and sterile alpha motif domain containing 1A (Anks1a), (10 ug)

Product Images

Circular map for RR212066

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Anks1a
• Synonyms: Anks1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR212066 representing NM_001107613
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGAAGGAGCAGGAGCTGCTGGAGGCGGCCCGCACCGGGCACCTCCCGGCCGTGGAGAAGCTGCTGT
  CCGGGAAGCGGCTCTCCTCCGGCTTCGGCGGCGGCGGCGGCGGCGGCTCCGGCAGCGGCGGCGGCTCCGG
  GGGCGGCGGCCTCGGCTCTTCCAGCCACCCTCTCTCCAGTCTGCTGAGCATGTGGAGAGGGCCGAATGTG
  AACTGCGTAGATAGCACCGGCTACACGCCACTACACCACGCTGCTTTGAATGGCCACAGGGATGTGGTCG
  AGGTTCTTCTGAGGAACGATGCGCTGACCAACGTTGCTGACTCGAAAGGCTGCTACCCACTGCACTTGGC
  AGCGTGGAAAGGAGATGCCCAGATAGTGCGGCTGCTCATCCACCAGGGGCCCTCGCACACCAGAGTCAAC
  GAACAGAACAACGACAACGAGACAGCCCTGCACTGCGCGGCACAGTACGGCCACACGGAGGTAGTGAAGG
  CCCTGCTGGAGGAGCTCACGGACCCCACCATGCGCAACAACAAATTTGAAACCCCGCTGGACCTGGCGGC
  GCTGTACGGGCGGCTGGAGGTGGTGAAGCTGCTGCTGAGTGCGCACCCCAATCTCCTGAGCTGCAGCACT
  AGGAAGCACACCCCCCTGCACTTGGCAGCAAGGAACGGCCACAAGGCCGTGGTGCAGGTGCTGCTGGACG
  CCGGCATGGACAGCAACTACCAGACGGAGATGGGCAGTGCTTTGCATGAAGCTGCTTTGTTCGGCAAGAC
  CGATGTGGTGCAAATCCTGCTGGCTGCAGGAATCGACGTCAACATCAAGGACAACCGTGGCCTGACTGCC
  CTTGACACTGTCCGAGACCTGCCTTCTCAGAAGAGCCAGCAGATAGCTGCGCTGATTGAAGACCACATGA
  CCGGAAGAAGAAGTGTGAAAGATGTAGACAAGACCTCCACAGCCCAGCTACCTCTCCTCTCCTGCACAGA
  CGCCATAGCCCCAAAGTCTCTGGGGAGTGTGGAGAAGGCAGTGACTGAGCCGACTCTTGATTCTGACATC
  CTCGCTGAGGAGGAGGGTCCCTACGAGGCGCTCTACAACGCTGTGTCCTGCCATTCCTTGGACAGCACGG
  CCAGTGGGCGATCGTCGGACAGAGACTCCGTGAACAAGGAGGCCGAGGCCACCGGAGCACGGGCTGCTGG
  AAGGGAACGCCCGCCACCTCCAGCAAAGCCGCCGCCTGATGAAGAGGAGGAAGAGCGCGTAGATAAGAAG
  TATCTTCCCTTGGCAGCTTCTGAGGGCCTGGCTTTGAGACCCAGAATGCAGGGCAGTGCCCCCCAGGAAG
  AGGAGGAGCACCCATATGAGCTGCTGCTCACAGCAGAGACCAAGAAGCTGGGGACCACAGACGGGAGGAC
  TGAAGACCACAGGCAGAGCGGCGGCAGCCGGAGCCAGGACTCTGTGGAGGGGCAGGACGGGCAGGTCCCG
  GAGCAGTTCTCAGGTCTCCTCCACGGCTCCTCCCCAGTGTGTGAGGTGGGGCAGGACCCTTTCCAGTTGC
  TTACTGTCCCCAGCCAGAACCACCCCGAGTCCTCCCAGCAGGACGCCTGCCAGGAGGCCAGCATGCAGCT
  AGAGGAGCCGGGCGAGCAAAGCACTGGAGCCCCGCAGCCTGGCATCCTGGACCAAAGCAAGAGAGCGGGC
  TTGTCCGCAGGCCTGACGGCCCTGGCCAGCAGAACATACCTCGACGCGTTGACTCACACAGTACCTCTGC
  ACCCTGCTGGTGCCGAGGAAGAAGACGGGTCCAGAAGCAGAGCCCCTCCCACCAGCAAGCCCAAAGCGGA
  GCTCAAACTCAGCCGCAGTTTGTCTAAGTCTGACTCTGACCTCCTGACCTGCTCCCCCACGGAGGACACC
  ACAATGGGGAGCCGCAGCGAGTCCCTGTCCAACTGCAGCATCGGGAAGAAGAGGCTAGAGAAGTCGCCTT
  CCTTTGCCTCCGAGTGGGATGAGATTGAGAAGATCATGAGCTCCATCGGCGAAGGGATTGACTTCTCTCA
  GGAGCAGCAGAAGATCTCAGGTTCTCGGACGCTGGAGCAGAGCGTCGGGGAGTGGCTGGAGTCGATCGGC
  CTGCAGCAGTATGAGAGCAAGCTGCTACTGAACGGCTTTGACGATGTCCGCTTCCTGGGGTCTAATGTGA
  TGGAAGACCAGGACCTGAGGGAGATTGGCATCAGTGACCCCCAGCACCGGCGGAAGCTGCTGCAGGCTGC
  ACGCTCCTTGCCCAAGGTGAAGGCCCTTGGCTATGATGGGGTCAGCCCCGCCTCAGTGCCCTCCTGGCTG
  GACTCGCTGGGGCTGCAGGACTATATCCACTCTTTCCTGTCCAGTGGCTACAGCTCCATCGACACCGTGA
  AGAACCTCTGGGAGCTAGAGCTCGTCAATGTCCTGAAGGTCCACCTGCTGGGCCACCGCAAACGCATTAT
  CGCCTCGCTTGCCGACAGGCCCTATGAGGAGCCGCCCCAGAAGCCTCCGAGGTTTTCCCAGCTAAGATGC
  CAGGATTTAATCTCCCAGACATCATCCCCGCTCAGCCAGAATGATTCCTGTACAGGGCGGTCGGCAGACC
  TGCTGCTGCCCTCGGTAGACACAAGCAGAAGGCGGCATGACAGCCTGCATGACCCCGGGACCACCTCTCG
  AGCAGAACACTTCCGGGTCCAGGAGGAGCCCAGCGAGAGCAAGCTGACCCTCCGTCCGCCCAGCCTGGCT
  GCCCCCTACGCCCCTGTACAAAGCTGGCAGCACCGGCCGGAAAAGCTCATCTTCGAATCCTGTGGTTATG
  AGGCCAATTACCTGGGCTCCATGCTGATCAAGGATCTGCGCGGGACCGAGTCCACCCAAGATGCCTGTGC
  CAAAATGCGGAAGTCGACGGAGCACATGAAGAAGATTCCCACCATCATCCTGTCCATCACATACAAGGGT
  GTCAAGTTCATGGACGCCTCCAACAAGAATGTCATCGCCGAGCATGAGATCCGGAACATTTCCTGCGCCG
  CGCAGGACCCGGAGGACCTCTGTACCTTTGCCTACATCACCAAGGACCTGCAGACCAGCCACCACTACTG
  CCATGTTTTCAGCACCGTGGATGTGAACCTGACCTACGAGATCATCCTGACCCTGGGGCAGGCATTTGAG
  GTGGCCTATCAGCTGGCCCTGCAGGCCCAGAAATCCAGGACCATGGCGGCCTCTGCAGCTTCAATGATTG
  AGACAAAATCTTCCAAACCGGTGCCTAAGCCTCGGGTCGGCATGAGGAAGTCTGCACTGGAACCTCCCGA
  TTCTGACCAAGAGGCCCCGTCCCACGCTAGTGTTTCCTGGATCGTGGACCCAAAGCCAGACTCGAAGCGG
  AGCCTCAGCACCAAC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR212066 representing NM_001107613
  Red=Cloning site Green=Tags(s)
  
  MGKEQELLEAARTGHLPAVEKLLSGKRLSSGFGGGGGGGSGSGGGSGGGGLGSSSHPLSSLLSMWRGPNV
  NCVDSTGYTPLHHAALNGHRDVVEVLLRNDALTNVADSKGCYPLHLAAWKGDAQIVRLLIHQGPSHTRVN
  EQNNDNETALHCAAQYGHTEVVKALLEELTDPTMRNNKFETPLDLAALYGRLEVVKLLLSAHPNLLSCST
  RKHTPLHLAARNGHKAVVQVLLDAGMDSNYQTEMGSALHEAALFGKTDVVQILLAAGIDVNIKDNRGLTA
  LDTVRDLPSQKSQQIAALIEDHMTGRRSVKDVDKTSTAQLPLLSCTDAIAPKSLGSVEKAVTEPTLDSDI
  LAEEEGPYEALYNAVSCHSLDSTASGRSSDRDSVNKEAEATGARAAGRERPPPPAKPPPDEEEEERVDKK
  YLPLAASEGLALRPRMQGSAPQEEEEHPYELLLTAETKKLGTTDGRTEDHRQSGGSRSQDSVEGQDGQVP
  EQFSGLLHGSSPVCEVGQDPFQLLTVPSQNHPESSQQDACQEASMQLEEPGEQSTGAPQPGILDQSKRAG
  LSAGLTALASRTYLDALTHTVPLHPAGAEEEDGSRSRAPPTSKPKAELKLSRSLSKSDSDLLTCSPTEDT
  TMGSRSESLSNCSIGKKRLEKSPSFASEWDEIEKIMSSIGEGIDFSQEQQKISGSRTLEQSVGEWLESIG
  LQQYESKLLLNGFDDVRFLGSNVMEDQDLREIGISDPQHRRKLLQAARSLPKVKALGYDGVSPASVPSWL
  DSLGLQDYIHSFLSSGYSSIDTVKNLWELELVNVLKVHLLGHRKRIIASLADRPYEEPPQKPPRFSQLRC
  QDLISQTSSPLSQNDSCTGRSADLLLPSVDTSRRRHDSLHDPGTTSRAEHFRVQEEPSESKLTLRPPSLA
  APYAPVQSWQHRPEKLIFESCGYEANYLGSMLIKDLRGTESTQDACAKMRKSTEHMKKIPTIILSITYKG
  VKFMDASNKNVIAEHEIRNISCAAQDPEDLCTFAYITKDLQTSHHYCHVFSTVDVNLTYEIILTLGQAFE
  VAYQLALQAQKSRTMAASAASMIETKSSKPVPKPRVGMRKSALEPPDSDQEAPSHASVSWIVDPKPDSKR
  SLSTN
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001107613
• ORF Size: 3375 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001107613.3, NP_001101083.1
• RefSeq Size: 5616 bp
• RefSeq ORF: 3378 bp
• Locus ID: 309639
• Cytogenetics: 20p12
• MW: 122.1 kDa