Hiatl3 (NM_001106467) Rat Tagged ORF Clone

CAT#: RR211817

Hiat1 (Myc-DDK-tagged ORF) - Rat hippocampus abundant gene transcript 1 (Hiat1), (10 ug)

Product Images

Circular map for RR211817

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hiatl3
• Synonyms: Hiat1; Mfsd14a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR211817 representing NM_001106467
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCCAGGGGAAGAAAAAGAAACGGGCCGCGAACCGCAGTATCATGCTGGCCAAAAAGATCATCATTA
  AGGACGGAGGCACGCCTCAAGGAATAGGCTCCCCTAGTGTTTATCATGCGGTTATCGTCATCTTTTTGGA
  GTTTTTTGCTTGGGGACTACTGACAGCACCCACATTGGTGGTATTGCATGAAACCTTCCCTAAACATACA
  TTTCTGATGAATGGCCTAATTCAAGGAGTAAAGGGTTTGTTGTCCTTCCTCAGTGCCCCACTTATTGGTG
  CTCTTTCTGATGTTTGGGGCCGAAAATCCTTCTTGCTGCTAACAGTGTTTTTCACATGTGCCCCTATTCC
  TCTAATGAAGATCAGCCCATGGTGGTACTTTGCAGTTATCTCTGTTTCTGGGGTTTTTGCAGTAACATTC
  TCTGTGGTATTTGCATATGTAGCAGATATAACTCAAGAGCATGAAAGAAGTATGGCTTATGGGCTGGTTT
  CCGCTACATTCGCTGCCAGCTTAGTTACCAGCCCTGCAATTGGAGCCTACCTTGGACGAGTGTATGGAGA
  CAGCTTAGTGGTGGTCCTTGCTACAGCAATAGCCCTGCTAGACATCTGTTTTATCCTTGTTGCTGTGCCA
  GAGTCATTGCCTGAGAAGATGAGGCCAGCATCGTGGGGCGCTCCCATTTCCTGGGAACAGGCTGACCCCT
  TTGCATCTTTAAAAAAAGTGGGCCAAGATTCCATAGTGCTGCTGATCTGTATCACGGTGTTTCTCTCCTA
  CCTACCCGAAGCAGGCCAGTACTCCAGCTTCTTCTTATACCTCAGACAGATAATGAAATTTTCCCCAGAA
  AGTGTGGCAGCTTTTATAGCGGTCCTTGGCATTCTGTCCATTATTGCACAGACCATAGTCTTGAGTTTAC
  TCATGAGGTCAATTGGAAATAAGAATACCATCTTACTCGGTCTGGGATTTCAAATATTGCAGCTGGCATG
  GTACGGCTTTGGTTCAGAACCTTGGATGATGTGGGCTGCTGGGGCAGTGGCAGCCATGTCTAGCATCACC
  TTTCCAGCTGTTAGTGCCCTGGTTTCACGGACTGCTGATGCCGATCAACAGGGTGTTGTTCAGGGAATGA
  TAACAGGAATCCGAGGACTGTGCAATGGTCTGGGACCAGCCCTTTATGGCTTCATTTTCTACATATTCCA
  TGTGGAACTTAAAGAACTGCCAATCACAGGGCCAGACCTGGGGACAAACACAAGCCCTCAGCACCACTTT
  GAACAGAATTCCATCATCCCTGGTCCCCCCTTCCTCTTTGGAGCCTGCTCAGTACTGCTGGCTCTGCTCG
  TTGCCTTGTTTATTCCGGAGCATACTAATTTAAGTTTAAGGTCCAGCAGTTGGAGGAAGCATTGTGGTGG
  TCACAGCCATCCTCACAGTACACAAGCGCCAGGAGAGGCCAAAGAACCTTTACTCCAGGACACGAATGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR211817 representing NM_001106467
  Red=Cloning site Green=Tags(s)
  
  MTQGKKKKRAANRSIMLAKKIIIKDGGTPQGIGSPSVYHAVIVIFLEFFAWGLLTAPTLVVLHETFPKHT
  FLMNGLIQGVKGLLSFLSAPLIGALSDVWGRKSFLLLTVFFTCAPIPLMKISPWWYFAVISVSGVFAVTF
  SVVFAYVADITQEHERSMAYGLVSATFAASLVTSPAIGAYLGRVYGDSLVVVLATAIALLDICFILVAVP
  ESLPEKMRPASWGAPISWEQADPFASLKKVGQDSIVLLICITVFLSYLPEAGQYSSFFLYLRQIMKFSPE
  SVAAFIAVLGILSIIAQTIVLSLLMRSIGNKNTILLGLGFQILQLAWYGFGSEPWMMWAAGAVAAMSSIT
  FPAVSALVSRTADADQQGVVQGMITGIRGLCNGLGPALYGFIFYIFHVELKELPITGPDLGTNTSPQHHF
  EQNSIIPGPPFLFGACSVLLALLVALFIPEHTNLSLRSSSWRKHCGGHSHPHSTQAPGEAKEPLLQDTNV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001106467
• ORF Size: 1470 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001106467.3, NP_001099937.1
• RefSeq Size: 2727 bp
• RefSeq ORF: 1473 bp
• Locus ID: 100134827
• Cytogenetics: 1q12
• MW: 53 kDa