Ezh1 (NM_001107051) Rat Tagged ORF Clone

CAT#: RR211701

Ezh1 (Myc-DDK-tagged ORF) - Rat enhancer of zeste homolog 1 (Drosophila) (Ezh1), (10 ug)

Product Images

Circular map for RR211701

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ezh1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR211701 representing NM_001107051
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATATAGCAAGTCCCCCAACTTCCAAATGCATCACATACTGGAAAAGAAAGGTGAAATCTGAATACA
  TGCGGCTGCGGCAGCTCAAACGGCTTCAGGCAAATATGGGGGCAAAGGCTCTGTATGTGGCAAATTTTGC
  AAAAGTTCAAGAAAAAACCCAAATCCTCAATGAAGAGTGGAAGAAACTTCGCGTCCAGCCTGTTCAGCCA
  ATGAAGCCTGTGAGTGGGCACCCTTTTCTGAAAAAGTGTACCATAGAGAGCATTTTCCCAGGTTTTGACA
  GCCAGGATATGTTGATGAGGTCTCTGAACACAGTTGCATTGGTTCCAATCATGTATTCCTGGTCCCCTCT
  CCAGCAGAACTTCATGGTGGAGGATGAGACGGTTTTGTGCAATATTCCCTACATGGGTGATGAGGTGAAG
  GAGGAAGACGAAACTTTCATCGAGGAGCTGATCAACAACTATGACGGCAAAGTCCATGGTGAAGAAGAGA
  TGATCCCTGGGTCTGTGCTGATCAGCGATGCTGTGTTTCTGGAGTTGGTAGATGCCCTCAATCAGTACTC
  GGATGAGGAGGAGGAAGGGCACAATGACACCTCCGATGGAAAGCAGGATGACAGCAAAGAGGACCTGCCA
  GTGACAAGAAAGCGGAAACGCCATGCTATTGAAGGCAACAAAAAGAGCTCCAAGAAACAGTTTCCAAATG
  ACATGATCTTCAGCGCCATTGCTTCCATGTTCCCCGAGAATGGTGTCCCTGACGACATGAAGGAGAGGTA
  TCGAGAGCTGACAGAGATGTCGGACCCCAATGCACTTCCCCCTCAGTGCACACCCAACATCGATGGCCCC
  AATGCTAAGTCAGTGCAGCGGGAGCAGTCTCTGCACTCTTTCCACACCCTGTTCTGTCGGCGCTGTTTTA
  AATACGACTGCTTCCTTCACCCTTTCCACGCCACCCCTAATGTGTACAAAAGGAAGAACAGAGAAATCAA
  GATTGAGCCAGAACCATGTGGTGCAGACTGCTTCCTTTGGCTGGAAGGAGCAAAGGAGTACGCCATGCTG
  CATAACCCTCGGTCCAAGTGCTCTGGGCGCCGCCGCCGAAGGCACCCAGTGGTCAGTGCTTCCTGTTCCA
  ATACATCCGCCTCTGCTATGGCCGAGACTAAAGAAGGAGACAGTGACAGAGACACTGGCAATGACTGGGC
  CTCCAGTTCTTCAGAGGCTAACTCTCGCTGTCAGACCCCCACGAAACAGAAAGCTAGTCCAGCCCCACCT
  CAGCTCTGTGTTGTGGAAGCTCCCTCAGAGCCCGTGGAATGGACTGGAGCTGAAGAATCTCTTTTCCGAG
  TCTTCCATGGCACCTACTTCAACAACTTCTGCTCAATAGCCAGGCTTCTGGGGACAAAGACATGCAAACA
  GGTCTTTCAGTTTGCAGTCAAAGAATCACTTATCCTGAAGCTGCCAACAGATGAGCTCATGAACCCTTCA
  CAGAAGAAGAAAAGAAAACACAGGTTGTGGGCTGCGCACTGCAGGAAAATTCAGCTGAAGAAAGACAACA
  ATTCTACACAAGTGTATAACTACCAACCCTGTGACCACCCGGACCGTCCCTGTGACAGCACATGCCCCTG
  CATCATGACCCAGAACTTTTGTGAAAAGTTCTGCCAGTGCAGCCCAGACTGCCAGAACCGCTTCCCTGGT
  TGTCGCTGTAAGACTCAGTGCAATACCAAGCAGTGTCCTTGCTACTTGGCCGTTCGTGAGTGTGACCCTG
  ACTTGTGCCTCACCTGCGGGGCCTCAGAGCACTGGGACTGTAAGGTGGTGTCTTGCAAAAACTGCAGCAT
  CCAGCGTGGCCTCAAAAAGCACCTGCTGCTGGCCCCTTCCGATGTGGCCGGATGGGGCACCTTCATCAAG
  GAGTCTGTGCAGAAGAACGAATTCATTTCTGAATACTGTGGTGAGCTCATCTCTCAGGATGAGGCTGATC
  GACGAGGGAAGGTCTATGATAAATATATGTCCAGCTTCCTCTTCAACCTCAACAATGATTTTGTAGTGGA
  TGCTACCCGGAAAGGAAACAAAATTCGCTTTGCAAACCATTCAGTGAACCCCAACTGTTATGCCAAAGTG
  GTCATGGTGAATGGAGATCACCGCATTGGGATCTTTGCCAAGAGAGCAATTCAGGCTGGCGAAGAGCTCT
  TCTTTGATTATAGGTACAGCCAAGCTGATGCCCTCAAGTATGTGGGCATCGAAAGGGAAACGGACGTCTT
  C
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR211701 representing NM_001107051
  Red=Cloning site Green=Tags(s)
  
  MDIASPPTSKCITYWKRKVKSEYMRLRQLKRLQANMGAKALYVANFAKVQEKTQILNEEWKKLRVQPVQP
  MKPVSGHPFLKKCTIESIFPGFDSQDMLMRSLNTVALVPIMYSWSPLQQNFMVEDETVLCNIPYMGDEVK
  EEDETFIEELINNYDGKVHGEEEMIPGSVLISDAVFLELVDALNQYSDEEEEGHNDTSDGKQDDSKEDLP
  VTRKRKRHAIEGNKKSSKKQFPNDMIFSAIASMFPENGVPDDMKERYRELTEMSDPNALPPQCTPNIDGP
  NAKSVQREQSLHSFHTLFCRRCFKYDCFLHPFHATPNVYKRKNREIKIEPEPCGADCFLWLEGAKEYAML
  HNPRSKCSGRRRRRHPVVSASCSNTSASAMAETKEGDSDRDTGNDWASSSSEANSRCQTPTKQKASPAPP
  QLCVVEAPSEPVEWTGAEESLFRVFHGTYFNNFCSIARLLGTKTCKQVFQFAVKESLILKLPTDELMNPS
  QKKKRKHRLWAAHCRKIQLKKDNNSTQVYNYQPCDHPDRPCDSTCPCIMTQNFCEKFCQCSPDCQNRFPG
  CRCKTQCNTKQCPCYLAVRECDPDLCLTCGASEHWDCKVVSCKNCSIQRGLKKHLLLAPSDVAGWGTFIK
  ESVQKNEFISEYCGELISQDEADRRGKVYDKYMSSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKV
  VMVNGDHRIGIFAKRAIQAGEELFFDYRYSQADALKYVGIERETDVF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001107051
• ORF Size: 2241 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001107051.1, NP_001100521.1
• RefSeq Size: 4245 bp
• RefSeq ORF: 2244 bp
• Locus ID: 303547
• Cytogenetics: 10q31
• MW: 85.3 kDa