Kcna5 (NM_012972) Rat Tagged ORF Clone

CAT#: RR211444

Kcna5 (Myc-DDK-tagged ORF) - Rat potassium voltage-gated channel, shaker-related subfamily, member 5 (Kcna5), (10 ug)

Product Images

Circular map for RR211444

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Kcna5
• Synonyms: Kv1; Kv1.5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR211444 representing NM_012972
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGATCTCCCTGGTGCCCCTGGAGAATGGCAGTGCCATGACCCTCAGAGGAGGAGGGGAGGCAGGGG
  CAAGCTGTGTGCAGACCCCCAGGGGAGAGTGTGGGTGCCCTCCGACGTCTGGACTCAATAATCAGTCCAA
  AGAAACACTGTTGAGGGGGCGCACAACGCTCGAGGATGCGAACCAGGGTGGACGGCCTTTGCCCCCTATG
  GCTCAGGAGCTGCCACAACCTAGAAGGCTATCTGCTGAGGATGAGGAGGGAGAAGGCGACCCTGGCCTGG
  GCACAGTGGAGGAGGACCAGGCTCCTCAGGATGCAGGGTCACTCCATCACCAGCGAGTCCTCATAAACAT
  CTCCGGGTTGCGTTTCGAGACGCAGCTGGGCACCCTGGCACAGTTTCCCAACACCCTCCTGGGGGACCCA
  GCCAAGCGCCTGCACTACTTCGACCCCTTGAGAAATGAATACTTCTTCGACCGCAACCGGCCCAGCTTCG
  ATGGCATTTTGTACTACTACCAGTCTGGGGGCCGCCTGCGCAGGCCCGTCAATGTCTCCCTGGATGTGTT
  TGCAGATGAGATCCGCTTTTACCAGCTGGGGGACGAGGCCATGGAGCGCTTCCGGGAGGATGAGGGCTTC
  ATCAAGGAAGAGGAGAAGCCCCTGCCCCGCAACGAGTTCCAGCGCCAGGTATGGCTTATCTTCGAATACC
  CAGAAAGCTCTGGGTCCGCAAGAGCCATCGCCATAGTGTCGGTCCTGGTCATTCTCATCTCTATCATCAC
  CTTCTGCCTGGAGACTCTGCCTGAGTTCAGGGATGAGCGGGAGCTGCTACGCCATCCCCCAGTGCCGCCC
  CAGCCCCCAGCCCCTGCCCCTGGGATCAATGGCAGCGTCTCTGGAGCACTTTCCTCTGGCCCTACGGTGG
  CTCCACTCTTGCCTAGGACACTGGCCGATCCATTCTTCATCGTGGAGACCACATGTGTGATCTGGTTCAC
  TTTTGAGTTGCTCGTGCGCTTCTTTGCCTGCCCCAGCAAGGCAGAATTCTCTCGGAATATCATGAACATC
  ATCGATGTCGTGGCCATCTTCCCCTACTTTATCACCCTGGGCACCGAGCTGGCAGAGCAACAACCAGGGG
  GAGGGGGTCAGAATGGGCAGCAGGCCATGTCCCTAGCCATCCTCAGGGTGATCCGCCTGGTGCGGGTGTT
  TCGGATCTTCAAGCTCTCCCGCCACTCCAAGGGACTGCAGATCCTGGGTAAGACCTTGCAAGCATCCATG
  CGGGAACTCGGGCTACTCATCTTCTTCCTCTTCATTGGAGTCATCCTCTTCTCCAGCGCTGTCTACTTCG
  CAGAGGCAGACAATCACGGGTCCCATTTCTCTAGTATCCCAGATGCCTTCTGGTGGGCAGTAGTCACTAT
  GACCACTGTAGGCTATGGAGACATGAGACCCATCACTGTAGGGGGCAAGATCGTGGGTTCACTGTGCGCC
  ATAGCTGGGGTCCTCACCATTGCCCTGCCTGTCCCCGTCATCGTCTCCAACTTTAATTACTTCTATCATC
  GGGAGACAGACCACGAGGAGCAGGCTGCCCTGAAGGAAGAACAAGGCAACCAGAGGCGGGAGTCTGGGCT
  GGACACAGGGGGTCAACGGAAGGTCAGCTGCAGCAAGGCCTCCTTTTGCAAGACTGGGGGTTCCCTGGAG
  AGTTCTGACAGTATCAGAAGGGGTAGCTGTCCTCTAGAAAAGTGTCACCTCAAGGCCAAGAGCAACGTGG
  ACTTGCGGAGGTCCCTGTATGCCCTCTGTCTGGACACTAGCCGTGAAACAGATTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR211444 representing NM_012972
  Red=Cloning site Green=Tags(s)
  
  MEISLVPLENGSAMTLRGGGEAGASCVQTPRGECGCPPTSGLNNQSKETLLRGRTTLEDANQGGRPLPPM
  AQELPQPRRLSAEDEEGEGDPGLGTVEEDQAPQDAGSLHHQRVLINISGLRFETQLGTLAQFPNTLLGDP
  AKRLHYFDPLRNEYFFDRNRPSFDGILYYYQSGGRLRRPVNVSLDVFADEIRFYQLGDEAMERFREDEGF
  IKEEEKPLPRNEFQRQVWLIFEYPESSGSARAIAIVSVLVILISIITFCLETLPEFRDERELLRHPPVPP
  QPPAPAPGINGSVSGALSSGPTVAPLLPRTLADPFFIVETTCVIWFTFELLVRFFACPSKAEFSRNIMNI
  IDVVAIFPYFITLGTELAEQQPGGGGQNGQQAMSLAILRVIRLVRVFRIFKLSRHSKGLQILGKTLQASM
  RELGLLIFFLFIGVILFSSAVYFAEADNHGSHFSSIPDAFWWAVVTMTTVGYGDMRPITVGGKIVGSLCA
  IAGVLTIALPVPVIVSNFNYFYHRETDHEEQAALKEEQGNQRRESGLDTGGQRKVSCSKASFCKTGGSLE
  SSDSIRRGSCPLEKCHLKAKSNVDLRRSLYALCLDTSRETDL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012972
• ORF Size: 1806 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012972.1, NP_037104.1
• RefSeq Size: 1809 bp
• RefSeq ORF: 1809 bp
• Locus ID: 25470
• UniProt ID: P19024
• Cytogenetics: 4q42
• MW: 66.6 kDa
• Gene Summary: voltage gated potassium channel; functions as a delayed rectifier-like K+ channel [RGD, Feb 2006]