Osgin1 (NM_138504) Rat Tagged ORF Clone

CAT#: RR211282

Osgin1 (Myc-DDK-tagged ORF) - Rat oxidative stress induced growth inhibitor 1 (Osgin1), (10 ug)

Product Images

Circular map for RR211282

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Osgin1
• Synonyms: Okl38
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR211282 representing NM_138504
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTCCTGGAGGCATGACTCCCTCAGGGCCAGCAGCTCCGATCCCCTCCCAGTAGTTATCATTGGCA
  ATGGTCCCTCGGGTATCTGTCTATCCTACCTGCTCTCTGGCTACATCCCCTATGTGAAACCAGGAGCTGT
  CCACCCGCACCCCCTGCTGCAGAGGAAGCTGACCGAGGCTCCAGGGGTCTCCATCTTGGATCAGGACCTA
  GAATACCTTTCCGAAGGCCTCGAAGGCCGAAGCCAGAGTCCTGTGGCCCTGCTCTTTGATGCCCTCCTAC
  GTCCTGACACAGACTTTGGAGGCAGCCTAGACTCTGTGCTCTCCTGGAAGCGGCAGAAGGACCGTGCCAT
  TCCCCACCTTGTCCTGGGACGGAACCTCCCCGGGGGTGCCTGGCATTCCATTGAAGGCTCCATGGTGACT
  TTGAGCCAAGGCCAGTGGATGAGTCTCCCAGACCTGCAGGTCAAGGACTGGATGCGGAAGAAATGCAGAG
  GCCTCCGTAACAGCAGAGCCACAGCCGGTGACATCGCCCACTACTACAGGGACTATGTGATCAAGAAGGG
  TCTCAGTCACAACTTTGTGTCTGGTGCCGTGGTCACTGCCGTGGAATGGGCTAAGTCTGAGCACTGCAGC
  CCTGAGGCCCAGGCACCCAGCCCCCTCTTCCAAGTGACAGGCTACCTGACTGCCAAGGACCACAGCCGCC
  AGCCCTTCTCACTGTGGGCCCATAACGTGGTCCTGGCCACGGGCACCTTTGACAGCCCAGCCATGCTAGG
  CATCCCGGGAGAGACCCTGCCTTTTGTCCACCACGAGCTGTCAGCCCTGGAGGCGGCCCTCCGGGCAGGC
  ACTGTGAACCCAACCTCAGACCCGGTGCTGATTGTGGGAGCTGGGCTATCTGCGGCCGACGCTGTCCTCT
  TTGCCCGTCACTACAACATCCAGGTGATCCATGCCTTTCGCCGGTCTGTGCACGACCCTGGCCTGGTGTT
  CAACCAGCTGCCCAAGATGCTGTACCCCGAGTACCACAAAGTGCAGCAGATGATGCGTGACCAGTCCATC
  TTGTCCCCCAGCCCCTACGAGGGCTACCGCAGCCTCCCAGAGCACCAGCCACTGCTCTTCAAGGAAGACC
  GTCAGGCAGTGTTCCAGGACCCACAGGGGGGCCAGCAGCTCTTTGGAGTCTCCATGGTGCTCGTCCTCAT
  CGGCTCCCACCCCGACCTCTCCTACCTCCCTAGGGCAGGGGCTGACCTGGCCATAGACCCCAGTCAGCCA
  CTGAGCCCCAAGAGGAACCCCATCGATGTGGACCCGTTCACCCATGAGAGCACTCAACAGGAGGGCCTGT
  ATGCCCTAGGGCCCCTGGCTGGGGACAACTTTGTGAGGTTCGTCCAGGGTGGAGCCCTGGCTGCTGCCAG
  CTCCCTGCTGAAGAAGGAGACCAGGAAGCCACCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR211282 representing NM_138504
  Red=Cloning site Green=Tags(s)
  
  MSSWRHDSLRASSSDPLPVVIIGNGPSGICLSYLLSGYIPYVKPGAVHPHPLLQRKLTEAPGVSILDQDL
  EYLSEGLEGRSQSPVALLFDALLRPDTDFGGSLDSVLSWKRQKDRAIPHLVLGRNLPGGAWHSIEGSMVT
  LSQGQWMSLPDLQVKDWMRKKCRGLRNSRATAGDIAHYYRDYVIKKGLSHNFVSGAVVTAVEWAKSEHCS
  PEAQAPSPLFQVTGYLTAKDHSRQPFSLWAHNVVLATGTFDSPAMLGIPGETLPFVHHELSALEAALRAG
  TVNPTSDPVLIVGAGLSAADAVLFARHYNIQVIHAFRRSVHDPGLVFNQLPKMLYPEYHKVQQMMRDQSI
  LSPSPYEGYRSLPEHQPLLFKEDRQAVFQDPQGGQQLFGVSMVLVLIGSHPDLSYLPRAGADLAIDPSQP
  LSPKRNPIDVDPFTHESTQQEGLYALGPLAGDNFVRFVQGGALAAASSLLKKETRKPP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_138504
• ORF Size: 1434 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138504.2, NP_612513.1
• RefSeq Size: 2355 bp
• RefSeq ORF: 1437 bp
• Locus ID: 171493
• Cytogenetics: 19q12
• MW: 52.3 kDa
• Gene Summary: may play a role in growth inhibition in mammary epithelial cells [RGD, Feb 2006]