Cacna1g (NM_031601) Rat Tagged ORF Clone
CAT#: RR211239
- TrueORF®
Cacna1g (Myc-DDK-tagged ORF) - Rat calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), (10 ug)
"NM_031601" in other vectors (1)
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Cacna1g |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR211239 representing NM_031601
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGAGGAGGAGGATGGAGCGGGCGCCGAGGAGTCGGGACAGCCCCGTAGCTTCACGCAGCTCAACG ACCTGTCCGGGGCCGGGGGCCGGCAGGGGCCGGGGTCGACGGAAAAGGACCCGGGCAGCGCGGACTCCGA GGCGGAGGGGCTGCCGTACCCGGCGCTAGCCCCGGTGGTTTTCTTCTACTTGAGCCAGGACAGCCGCCCG CGGAGCTGGTGTCTCCGCACGGTCTGTAACCCGTGGTTCGAGCGAGTCAGTATGCTGGTCATTCTTCTCA ACTGTGTGACTCTGGGTATGTTCAGGCCGTGTGAGGACATTGCCTGTGACTCCCAGCGCTGCCGGATCCT GCAGGCCTTCGATGACTTCATCTTTGCCTTCTTTGCTGTGGAAATGGTGGTGAAGATGGTGGCCTTGGGC ATCTTTGGGAAGAAATGTTACCTGGGAGACACTTGGAACCGGCTTGACTTTTTCATTGTCATTGCAGGGA TGCTGGAGTATTCGCTGGACCTGCAGAACGTCAGCTTCTCCGCAGTCAGGACAGTCCGTGTGCTGCGACC GCTCAGGGCCATTAACCGGGTGCCCAGCATGCGCATTCTCGTCACATTACTGCTGGACACCTTGCCTATG CTGGGCAACGTCCTGCTGCTCTGTTTCTTCGTCTTTTTCATCTTTGGCATCGTGGGCGTCCAGCTGTGGG CAGGACTGCTTCGCAACCGATGCTTCCTCCCCGAGAACTTCAGCCTCCCCCTGAGCGTGGACCTGGAGCC TTATTACCAGACAGAGAATGAGGACGAGAGCCCCTTCATCTGCTCTCAGCCTCGGGAGAATGGCATGAGA TCCTGCAGGAGTGTGCCCACACTGCGTGGGGAAGGCGGTGGTGGCCCACCCTGCAGTCTGGACTATGAGA CCTATAACAGTTCCAGCAACACCACCTGTGTCAACTGGAACCAGTACTATACCAACTGCTCTGCGGGCGA GCACAACCCCTTCAAAGGCGCCATCAACTTTGACAACATTGGCTATGCCTGGATCGCCATCTTCCAGGTC ATCACACTGGAGGGCTGGGTCGACATCATGTACTTCGTAATGGACGCTCACTCCTTCTACAACTTCATCT ACTTCATTCTTCTCATCATCGTGGGCTCCTTCTTCATGATCAACCTGTGCCTGGTGGTGATTGCCACGCA GTTCTCCGAGACCAAACAGCGGGAGAGTCAGCTGATGCGGGAGCAGCGTGTACGATTCCTGTCCAATGCT AGCACCCTGGCAAGCTTCTCTGAGCCAGGCAGCTGCTATGAGGAGCTACTCAAGTACCTGGTGTACATCC TCCGAAAAGCAGCCCGAAGGCTGGCCCAGGTCTCTAGGGCTATAGGCGTGCGGGCTGGGCTGCTCAGCAG CCCAGTGGCCCGTAGTGGGCAGGAGCCCCAGCCCAGTGGCAGCTGCACTCGCTCACACCGTCGTCTGTCT GTCCACCACCTGGTCCACCACCATCACCACCACCATCACCACTACCACCTGGGTAATGGGACGCTCAGAG TTCCCCGGGCCAGCCCAGAGATCCAGGACAGGGATGCCAATGGGTCTCGCCGGCTCATGCTACCACCACC CTCTACACCCACTCCCTCTGGGGGCCCTCCGAGGGGTGCGGAGTCTGTACACAGCTTCTACCATGCTGAC TGCCACTTGGAGCCAGTCCGTTGCCAGGCACCCCCTCCCAGATGCCCATCGGAGGCATCTGGTAGGACTG TGGGTAGTGGGAAGGTGTACCCCACTGTGCATACCAGCCCTCCACCAGAGATACTGAAGGATAAAGCACT AGTGGAGGTGGCCCCCAGCCCTGGGCCCCCCACCCTCACCAGCTTCAACATCCCACCTGGGCCCTTCAGC TCCATGCACAAGCTCCTGGAGACACAGAGTACGGGAGCCTGCCATAGCTCCTGCAAAATCTCCAGCCCTT GCTCCAAGGCAGACAGTGGAGCCTGCGGGCCGGACAGTTGTCCCTACTGTGCCCGGACAGGAGCAGGAGA GCCAGAGTCCGCTGACCATGTCATGCCTGACTCAGACAGCGAGGCTGTGTATGAGTTCACACAGGACGCT CAGCACAGTGACCTCCGGGATCCCCACAGCCGGCGGCGACAGCGGAGCCTGGGCCCAGATGCAGAGCCTA GTTCTGTGCTGGCTTTCTGGAGGCTGATCTGTGACACATTCCGGAAGATCGTAGATAGCAAATACTTTGG CCGGGGAATCATGATCGCCATCCTGGTCAATACACTCAGCATGGGCATCGAGTACCACGAGCAGCCCGAG GAGCTCACCAACGCCCTGGAAATCAGCAACATCGTCTTCACCAGCCTCTTCGCCTTGGAGATGCTGCTGA AACTGCTTGTCTACGGTCCCTTTGGCTACATTAAGAATCCCTACAACATCTTTGATGGTGTCATTGTGGT CATCAGTGTGTGGGAGATTGTGGGCCAGCAGGGAGGTGGCCTGTCGGTGCTGCGGACCTTCCGCCTGATG CGGGTGCTGAAGCTGGTGCGCTTCCTGCCGGCCCTGCAGCGCCAGCTCGTGGTGCTCATGAAGACCATGG ACAACGTGGCCACCTTCTGCATGCTCCTCATGCTGTTCATCTTCATCTTCAGCATCCTGGGCATGCATCT CTTTGGTTGCAAGTTCGCATCTGAACGGGATGGGGACACGTTGCCAGACCGGAAGAATTTCGACTCCCTG CTCTGGGCCATCGTCACTGTCTTTCAGATTCTGACTCAGGAAGACTGGAATAAAGTCCTCTACAACGGCA TGGCCTCCACATCGTCTTGGGCTGCTCTTTACTTCATCGCCCTCATGACTTTTGGCAACTATGTGCTCTT TAACCTGCTGGTGGCCATTCTTGTGGAAGGCTTCCAGGCAGAGGGAGATGCCACCAAGTCTGAGTCAGAG CCTGATTTCTTTTCGCCCAGTGTGGATGGTGATGGGGACAGAAAGAAGCGCTTGGCCCTGGTGGCTTTGG GAGAACACGCGGAACTACGAAAGAGCCTTTTGCCACCCCTCATCATCCATACGGCTGCGACACCAATGTC ACTACCCAAGAGCTCCAGCACAGGTGTGGGGGAAGCACTGGGCTCTGGCTCTCGACGTACCAGTAGCAGT GGGTCCGCTGAGCCTGGAGCTGCCCACCATGAGATGAAATCTCCGCCAAGTGCCCGCAGCTCCCCGCACA GTCCCTGGAGTGCGGCAAGCAGCTGGACCAGCAGGCGCTCCAGCAGGAACAGCCTGGGCCGGGCCCCCAG CCTAAAGCGGAGGAGCCCGAGCGGGGAGCGGAGGTCCCTGCTGTCTGGAGAGGGCCAGGAGAGTCAGGAT GAGGAGGAAAGTTCAGAAGAGGACCGGGCCAGCCCAGCAGGCAGTGACCATCGCCACAGGGGTTCCTTGG AACGTGAGGCCAAGAGTTCCTTTGACCTGCCTGACACTCTGCAGGTGCCGGGGCTGCACCGCACAGCCAG CGGCCGGAGCTCTGCCTCTGAGCACCAAGACTGTAATGGCAAGTCGGCTTCAGGGCGTTTGGCCCGCACC CTGAGGACTGATGACCCCCAACTGGATGGGGATGATGACAATGATGAGGGAAATCTGAGCAAAGGGGAAC GCATACAAGCCTGGGTCAGATCCCGGCTTCCTGCCTGTTGCCGAGAGCGAGATTCCTGGTCGGCCTATAT CTTTCCTCCTCAGTCAAGGTTTCGTCTCCTGTGTCACCGGATCATCACCCACAAGATGTTTGACCATGTG GTCCTCGTCATCATCTTCCTCAACTGTATCACCATCGCTATGGAGCGCCCCAAAATTGACCCCCACAGCG CTGAGCGCATCTTCCTGACCCTCTCCAACTACATCTTCACGGCAGTCTTTCTAGCTGAAATGACAGTGAA GGTGGTGGCACTGGGCTGGTGCTTTGGGGAGCAGGCCTACCTGCGCAGCAGCTGGAATGTGCTGGACGGC TTGCTGGTGCTCATCTCCGTCATCGACATCCTGGTCTCCATGGTCTCCGACAGCGGCACCAAGATCCTTG GCATGCTGAGGGTGCTGCGGCTGCTGCGGACCCTGCGTCCACTCAGGGTCATCAGCCGGGCCCAGGGACT GAAGCTGGTGGTAGAGACTCTGATGTCATCCCTCAAACCCATTGGCAACATTGTGGTCATTTGCTGTGCC TTCTTCATCATTTTTGGAATTCTCGGGGTGCAGCTCTTCAAAGGGAAGTTCTTCGTGTGTCAGGGTGAGG ACACCAGGAACATCACTAACAAATCCGACTGCGCTGAGGCCAGCTACCGATGGGTCCGGCACAAGTACAA CTTTGACAACCTGGGCCAGGCTCTGATGTCCCTGTTTGTGCTGGCCTCCAAGGATGGTTGGGTTGACATC ATGTATGATGGGCTGGATGCTGTGGGTGTGGATCAGCAGCCCATCATGAACCACAACCCCTGGATGCTGC TATACTTCATCTCCTTCCTCCTCATCGTGGCCTTCTTTGTCCTGAACATGTTTGTGGGCGTGGTGGTGGA GAACTTCCATAAGTGCAGACAGCACCAGGAGGAGGAGGAGGCGAGGCGGCGTGAGGAGAAGCGACTACGG AGGCTGGAGAAAAAGAGAAGGAGTAAGGAGAAGCAGATGGCCGAAGCCCAGTGCAAGCCCTACTACTCTG ACTACTCGAGATTCCGGCTCCTTGTCCACCACCTGTGTACCAGCCACTACCTGGACCTCTTCATCACTGG TGTCATCGGGCTGAACGTGGTCACTATGGCCATGGAACATTACCAGCAGCCCCAGATCCTGGACGAGGCT CTGAAGATCTGCAATTACATCTTTACCGTCATCTTTTGTCCTTTGAGTCAGTTTTCAAACTTGTGGGCCT TTGGCTTCCGCCGTTTCTTCCAGGACAGGTGGAACCAGCTGGACCTGGCTATTGTGCTTCTGTCCATCAT GGGCATCACACTGGAGGAGATTGAGGTCAATGCTTCGCTGCCCATCAACCCCACCATCATCCGTATCATG AGGGTGCTCCGCATTGCTCGAGTTCTGAAGCTGTTGAAGATGGCTGTGGGCATGCGGGCACTGCTGGACA CGGTGATGCAGGCCCTGCCCCAGGTGGGGAACCTGGGACTTCTCTTCATGTTATTGTTTTTCATCTTTGC AGCTCTGGGCGTGGAGCTCTTTGGAGACCTGGAGTGTGATGAGACACACCCTTGTGAGGGCTTGGGTCGG CATGCCACCTTTAGGAACTTTGGTATGGCCTTTCTGACCCTCTTCCGAGTCTCCACTGGTGACAACTGGA ATGGTATTATGAAGGACACCCTCCGGGACTGTGACCAGGAGTCCACCTGCTACAACACTGTCATCTCCCC TATCTACTTTGTGTCCTTCGTGCTGACGGCCCAGTTTGTGCTGGTCAACGTGGTCATAGCTGTGCTGATG AAGCACCTGGAAGAAAGCAACAAAGAGGCCAAGGAGGAGGCCGAGCTCGAGGCCGAGCTGGAGCTGGAGA TGAAGACGCTCAGCCCGCAGCCCCACTCCCCGCTGGGCAGCCCCTTCCTCTGGCCCGGGGTGGAGGGTGT CAACAGTCCTGACAGCCCTAAGCCTGGGGCTCCACACACCACTGCCCACATTGGAGCAGCCTCGGGCTTC TCCCTTGAGCACCCCACGATGGTACCCCACCCCGAGGAGGTGCCAGTCCCCCTAGGACCAGACCTGCTGA CTGTGAGGAAGTCTGGTGTCAGCCGGACGCACTCTCTGCCCAATGACAGCTACATGTGCCGCAATGGGAG CACTGCTGAGAGATCCCTAGGACACAGGGGCTGGGGGCTCCCCAAAGCCCAGTCAGGCTCCATCTTGTCC GTTCACTCCCAACCAGCAGACACCAGCTGCATCCTACAGCTTCCCAAAGATGTGCACTATCTGCTCCAGC CTCATGGGGCCCCCACCTGGGGCGCCATCCCTAAACTACCCCCACCTGGCCGCTCCCCTCTGGCTCAGAG GCCTCTCAGGCGCCAGGCAGCAATAAGGACTGACTCCCTGGATGTGCAGGGCCTGGGTAGCCGGGAAGAC CTGTTGTCAGAGGTGAGTGGGCCCTCCTGCCCTCTGACCCGGTCCTCATCCTTCTGGGGCGGGTCGAGCA TCCAGGTGCAGCAGCGTTCCGGCATCCAGAGCAAAGTCTCCAAGCACATCCGCCTGCCAGCCCCTTGCCC AGGCCTGGAACCCAGCTGGGCCAAGGACCCTCCAGAGACCAGAAGCAGCTTAGAGCTGGACACGGAGCTG AGCTGGATTTCAGGAGACCTCCTTCCCAGCAGCCAGGAAGAACCCCTGTCCCCACGGGACCTGAAGAAGT GCTACAGTGTAGAGACCCAGAGCTGCAGGCGCAGGCCTGGGTCCTGGCTAGATGAACAGCGGAGACACTC CATTGCTGTCAGCTGTCTGGACAGCGGCTCCCAACCCCGCCTATGTCCAAGCCCCTCAAGCCTCGGGGGC CAACCTCTTGGGGGTCCTGGGAGCCGGCCTAAGAAAAAACTCAGCCCACCCAGTATCTCTATAGACCCCC CGGAGAGCCAGGGCTCTCGGCCCCCATGCAGTCCTGGTGTCTGCCTCAGGAGGAGGGCGCCGGCCAGTGA CTCTAAGGATCCCTCGGTCTCCAGCCCCCTTGACAGCACGGCTGCCTCACCCTCCCCAAAGAAAGACACG CTGAGTCTCTCTGGTTTGTCTTCTGACCCAACAGACATGGACCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR211239 representing NM_031601
Red=Cloning site Green=Tags(s) MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRP RSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALG IFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPM LGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMR SCRSVPTLRGEGGGGPPCSLDYETYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQV ITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNA STLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAIGVRAGLLSSPVARSGQEPQPSGSCTRSHRRLS VHHLVHHHHHHHHHYHLGNGTLRVPRASPEIQDRDANGSRRLMLPPPSTPTPSGGPPRGAESVHSFYHAD CHLEPVRCQAPPPRCPSEASGRTVGSGKVYPTVHTSPPPEILKDKALVEVAPSPGPPTLTSFNIPPGPFS SMHKLLETQSTGACHSSCKISSPCSKADSGACGPDSCPYCARTGAGEPESADHVMPDSDSEAVYEFTQDA QHSDLRDPHSRRRQRSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPE ELTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLM RVLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSL LWAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEGDATKSESE PDFFSPSVDGDGDRKKRLALVALGEHAELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSS GSAEPGAAHHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQD EEESSEEDRASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLART LRTDDPQLDGDDDNDEGNLSKGERIQAWVRSRLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHV VLVIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDG LLVLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCA FFIIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDI MYDGLDAVGVDQQPIMNHNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLR RLEKKRRSKEKQMAEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEA LKICNYIFTVIFCPLSQFSNLWAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIM RVLRIARVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGR HATFRNFGMAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLM KHLEESNKEAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASGF SLEHPTMVPHPEEVPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILS VHSQPADTSCILQLPKDVHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSRED LLSEVSGPSCPLTRSSSFWGGSSIQVQQRSGIQSKVSKHIRLPAPCPGLEPSWAKDPPETRSSLELDTEL SWISGDLLPSSQEEPLSPRDLKKCYSVETQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGG QPLGGPGSRPKKKLSPPSISIDPPESQGSRPPCSPGVCLRRRAPASDSKDPSVSSPLDSTAASPSPKKDT LSLSGLSSDPTDMDP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_031601 |
ORF Size | 6765 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_031601.4, NP_113789.3 |
RefSeq Size | 7657 bp |
RefSeq ORF | 6768 bp |
Locus ID | 29717 |
Cytogenetics | 10q26 |
MW | 250.3 kDa |
Gene Summary | low voltage-activated calcium channel alpha subunit; involved in calcium ion transport in nerve, muscle and edocrine cells [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN211239 | Cacna1g (untagged ORF) - Rat calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), (10 ug) |
USD 2,048.00 |
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