Abcb7 (NM_212518) Rat Tagged ORF Clone

CAT#: RR211165

Abcb7 (Myc-DDK-tagged ORF) - Rat ATP-binding cassette, sub-family B (MDR/TAP), member 7 (Abcb7), nuclear gene encoding mitochondrial protein, (10 ug)

Product Images

Circular map for RR211165

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Abcb7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR211165 representing NM_212518
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCTGCTCGCGATACATTCTTGGCGCTGGGCAGCCGCGGCGGTCGCTTTCGAAAAGCACAAGCATT
  CGGCAGTTCTGACCCGGTCTCTAGTCTCCATCTGCGGCTCAGGCCTGCGGTGGAGTTCGTACCAGAGCGG
  CGCGTCAGGAAGCGCTCGGCTGTCCCAGACTACAGAATCATTAAGAAATTCTACACAGCAGAGATGGGAA
  AAAAACAACTCAAGACAGTTACTAGATGCTTCAAAGGTTCTTCAGGCATGGCCATTGATAGAAAAGAGAA
  CATGTTGGCATGGGCACGCAGGAGGAGGACTCCACACAGACCCAAAAGAAGGGTTAAAGGATGTTGATAC
  TAGAAAAATCATTAAAGCCATGCTTTCTTATGTGTGGCCCAAAGACAGGCCTGATCTGCGAGCCAGAGTT
  GCCATTTCCCTGGGATTTCTGGGTGGTGCAAAGGCCATGAATATTGTGGTTCCTTTCATGTTTAAATATG
  CTGTAGACAGCCTCAACCAGATGTCGGGAAACATGCTGAACCTGAGTGATGCACCAAATACAGTTGCAAC
  CATGGCAACAGCAGTTCTGATTGGCTATGGTGTATCAAGAGCCGGGGCTGCCTTTTTCAATGAAGTCCGA
  AATGCAGTATTTGGCAAAGTAGCACAAAATTCAATCCGAAGAATAGCCAAAAATGTATTTCTCCATCTTC
  ACAACTTGGATCTGGGTTTCCATCTGAGCAGACAGACAGGAGCCTTATCTAAGGCTATTGACAGAGGGAC
  AAGGGGCATTAGTTTTGTCCTCAGTGCTTTAGTATTTAATCTTCTCCCTATTGTGTTTGAGATGACGCTT
  GTCAGTAGTGTTTTGTATTACAAATGTGGGGCCCAGTTTGCATTGGTAACCCTGGGAACACTTGGTGCAT
  ATACAGCATTCACAGTTGCAGTTACACGGTGGAGAACTAGATTTAGAATAGAAATGAACAAAGCTGATAA
  CGATGCAGGGAACGCTGCTATTGACTCACTGCTGAATTATGAAACTGTGAAGTATTTTAACAATGAAAAA
  TATGAAGCACAAAGATATGATGGATTCTTGAAGACATATGAGACTGCTTCATTGAAAAGTACCTCTACTC
  TGGCTATGCTGAATTTTGGCCAAAGTGCTATTTTCAGTGTTGGATTAACAGCTATCATGGTGCTTGCCAG
  TCAGGGAATTGTGGCAGGTGCCCTTACTGTTGGAGATCTAGTAATGGTGAATGGACTGCTTTTTCAACTT
  TCATTACCCCTTAACTTCTTGGGAACTGTATATAGAGAGACACGGCAAGCACTCATAGATATGAATACCT
  TGTTTACTCTGCTCAAGGTAGACACGCGGATTAAAGACAAAGCGATGGCATCTCCCCTTCAAATAACACC
  ACAGACAGCCACGGTGGCCTTTGATAATGTGCATTTTGAGTACATTGAAGGACAGAAAGTCCTTAGCGGA
  GTATCTTTTGAAGTCCCTGCAGGAAAGAAAGTGGCCATTGTAGGAGGTAGTGGGTCAGGAAAAAGCACGA
  TAGTGAGGCTGCTGTTTCGCTTCTATGAGCCTCAAAAGGGTAGCATTTACCTTGCTGGTCAAAATATTCA
  AGATGTGAGCCTGGAAAGTCTTCGGCGTGCAGTGGGAGTAGTACCTCAGGATGCTGTCCTCTTCCATAAT
  ACTATCTACTACAACCTCTTATATGGAAACATCAATGCGTCACCAGAGGAAGTATATGCAGTCGCAAAAT
  TGGCTGGTCTTCATGATGCAATTCTTCGAATGCCACATGGATATGACACACAAGTAGGAGAACGAGGACT
  CAAGTTATCAGGAGGAGAAAAGCAGAGGGTAGCGATTGCAAGAGCCATTTTGAAGGATCCCCCAGTTATT
  CTCTATGATGAAGCTACTTCATCATTAGATTCGATTACTGAAGAGACTATTCTTGGTGCCATGAGGGATG
  TGGTGAAGCACAGAACTTCTATTTTCATCGCACATAGATTGTCAACAGTGGTTGATGCAGATGAAATCAT
  TGTCCTGAGCCAGGGAAAAGTAGCTGAACGTGGTACCCACTATGGTCTGCTTGCTAACTCTAGCAGTATC
  TATTCAGAGATGTGGCATACACAGAGCACCCGCATACAGAACCATGATAACCTTGGATGGGATGCAAAGA
  AAGAGAGTCTCTCTAAAGAGGAGGAGAGAAAGAAGCTCCAAGAAGAGATTGTCAACAGCGTGAAAGGCTG
  TGGAAATTGCTCCTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR211165 representing NM_212518
  Red=Cloning site Green=Tags(s)
  
  MALLAIHSWRWAAAAVAFEKHKHSAVLTRSLVSICGSGLRWSSYQSGASGSARLSQTTESLRNSTQQRWE
  KNNSRQLLDASKVLQAWPLIEKRTCWHGHAGGGLHTDPKEGLKDVDTRKIIKAMLSYVWPKDRPDLRARV
  AISLGFLGGAKAMNIVVPFMFKYAVDSLNQMSGNMLNLSDAPNTVATMATAVLIGYGVSRAGAAFFNEVR
  NAVFGKVAQNSIRRIAKNVFLHLHNLDLGFHLSRQTGALSKAIDRGTRGISFVLSALVFNLLPIVFEMTL
  VSSVLYYKCGAQFALVTLGTLGAYTAFTVAVTRWRTRFRIEMNKADNDAGNAAIDSLLNYETVKYFNNEK
  YEAQRYDGFLKTYETASLKSTSTLAMLNFGQSAIFSVGLTAIMVLASQGIVAGALTVGDLVMVNGLLFQL
  SLPLNFLGTVYRETRQALIDMNTLFTLLKVDTRIKDKAMASPLQITPQTATVAFDNVHFEYIEGQKVLSG
  VSFEVPAGKKVAIVGGSGSGKSTIVRLLFRFYEPQKGSIYLAGQNIQDVSLESLRRAVGVVPQDAVLFHN
  TIYYNLLYGNINASPEEVYAVAKLAGLHDAILRMPHGYDTQVGERGLKLSGGEKQRVAIARAILKDPPVI
  LYDEATSSLDSITEETILGAMRDVVKHRTSIFIAHRLSTVVDADEIIVLSQGKVAERGTHYGLLANSSSI
  YSEMWHTQSTRIQNHDNLGWDAKKESLSKEEERKKLQEEIVNSVKGCGNCSC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_212518
• ORF Size: 2256 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_212518.1, NP_997683.1
• RefSeq Size: 2368 bp
• RefSeq ORF: 2259 bp
• Locus ID: 302395
• UniProt ID: Q704E8
• Cytogenetics: Xq22
• MW: 82.6 kDa
• Gene Summary: mouse homolog plays a role in heme biosynthesis in erythroid cells; regulates expression of both the mitochondrial iron-sulfur-containing protein ferrochelatase and the cytosolic iron-sulfur containing protein thioredoxin [RGD, Feb 2006]