Brd2 (NM_212495) Rat Tagged ORF Clone

CAT#: RR211139

Brd2 (Myc-DDK-tagged ORF) - Rat bromodomain containing 2 (Brd2), (10 ug)

Product Images

Circular map for RR211139

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Brd2
• Synonyms: Ring3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR211139 representing NM_212495
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCAAAACGTGACTCCCCACAAGCTCCCTGGGGAAGGGAATGCAGGGTTACTGGGGCTGGGCCCAG
  AGGCAGCAGCACCAGGGAAAAGGATTCGAAAGCCTTCGCTGTTGTATGAGGGATTCGAGAGCCCCACAAT
  GGCTTCTGTACCAGCTTTACAACTGGCCCCTGCCAACCCACCCCCTCCTGAGGTGTCCAATCCCAAAAAG
  CCAGGACGAGTAACAAACCAACTGCAGTACCTGCACAAGGTAGTAATGAAGGCTCTGTGGAAGCATCAGT
  TTGCATGGCCATTCCGGCAGCCTGTGGACGCTGTGAAGCTGGGTCTGCCGGATTATCACAAAATTATCAA
  ACAGCCCATGGACATGGGAACTATCAAGAGGAGACTTGAAAACAATTACTACTGGGCTGCCTCAGAATGT
  ATGCAGGATTTTAACACCATGTTTACCAACTGTTATATTTACAACAAGCCCACGGATGATATTGTCCTAA
  TGGCACAGACACTGGAAAAGATCTTCTTACAGAAAGTGGCATCGATGCCACAAGAGGAGCAAGAGCTGGT
  GGTGACTATCCCTAAGAACAGCCATAAGAAGGGGGCCAAGTTAGCAGCACTCCAGGGCAGTATTACCAGT
  GCCCATCAGGTGCCTGCTGTCTCTTCTGTGTCGCATACAGCCCTGTATACACCACCACCTGAAATACCTA
  CCACTGTCCTCAACATTCCCCACCCATCAGTCATCTCGTCTCCCCTTCTAAAGTCCCTGCATTCTGCTGG
  GCCCCCACTCCTTGCTGTATCAGCAGCTCCTCCAGCTCAGCCCCTTGCCAAGAAAAAAGGCGTTAAACGG
  AAAGCAGATACTACCACCCCTACACCTACAGCCATCCTGGCTCCCGGTTCCCCAGCTAGTCCTCCTGGGA
  GTCTTGAGCCAAAGGCAGCACGGCTCCCTCCTATGCGCAGAGAGAGTGGCCGCCCAATCAAGCCCCCACG
  AAAAGACTTGCCTGACTCGCAACAGCAACACCAGAGCTCTAAGAAAGGAAAGCTGTCAGAACAGTTAAAA
  CACTGCAATGGCATCCTGAAGGAGCTGCTCTCTAAGAAGCATGCTGCCTATGCCTGGCCTTTCTATAAAC
  CAGTGGACGCTTCTGCTCTTGGCCTTCATGACTACCATGACATCATTAAGCACCCCATGGACCTCAGCAC
  TGTCAAGCGGAAGATGGAGAACCGCGATTACCGGGATGCACAGGAGTTTGCTGCTGATGTACGGCTCATG
  TTCTCCAACTGCTATAAGTACAATCCTCCAGACCACGATGTCGTGGCTATGGCCCGGAAGTTACAGGATG
  TGTTTGAGTTCCGCTATGCCAAGATGCCAGATGAGCCACTGGAACCAGGACCTTTACCTGTCTCTACTGC
  CTTGCCTCCCGGGTTGGCCAAATCCTCTTCAGAGTCCTCCAGTGAGGAAAGTAGCAGTGAGAGCTCCTCT
  GAGGAAGAGGAGGAAGAGGATGAGGAAGATGAGGAGGAGGAGAGTGAAAGCTCAGACTCCGAGGAAGAAA
  GGGCTCATCGCCTGGCAGAGCTGCAAGAGCAGCTTCGGGCAGTTCATGAACAACTGGCTGCCCTATCCCA
  GGGCCCGATATCTAAACCCAAGCGGAAGAGAGAGAAAAAGGAAAAAAAAAAGAAACGGAAGGCAGAGAAA
  CATCGTGGCCGAATTGGGATTGATGAAGATGATAAGGGGCCTAGGGCCCCTCGCCCACTTCAGCCCAAGA
  AATCTAAGAAAGCAGGTGGTGGGGGTAGCAATGCTACTACACTCAGCCATCCTGGCTTCGGGACTTCTGC
  AGGAAGTAGCAACAAGCTGCCTAAAAAGGCTCAGAAGACTGCCCCACCTGTCCTTCCCACTGGCTATGAT
  TCGGAGGAGGAGGAAGAAAGCAGGCCCATGAGTTATGATGAAAAGAGACAGCTAAGCCTGGATATCAATA
  AGTTACCTGGGGAAAAGCTGGGTCGAGTTGTACATATCATCCAAGCCAGAGAGCCTTCTCTACGTGATTC
  AAATCCAGAAGAAATTGAGATTGATTTTGAAACACTCAAGCCGTCCACACTTAGGGAGCTTGAACGATAC
  GTTTTATCCTGCCTTCGAAAGAAACCCCGGAAGCCCTATACTATTAGAAAACCTGTGGGGAAAACAAAGG
  AGGAACTGGCTTTGGAGAAGAAGCGGGAACTAGAGAAGCGACTGCAGGATGTCAGTGGACAGCTTAACTC
  CACCAAAAAACCCCCCAAGAAAGCGAGTGAGAAGACAGAGTCATCTGCACAGCAAGTGGCAGTGTCCCGT
  CTCAGTGCTTCTAGTTCGAGCTCAGACTCCAGCTCCTCCTCCTCGTCCTCATCTTCTTCAGACACCAGCG
  ATTCAGACTCGGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR211139 representing NM_212495
  Red=Cloning site Green=Tags(s)
  
  MLQNVTPHKLPGEGNAGLLGLGPEAAAPGKRIRKPSLLYEGFESPTMASVPALQLAPANPPPPEVSNPKK
  PGRVTNQLQYLHKVVMKALWKHQFAWPFRQPVDAVKLGLPDYHKIIKQPMDMGTIKRRLENNYYWAASEC
  MQDFNTMFTNCYIYNKPTDDIVLMAQTLEKIFLQKVASMPQEEQELVVTIPKNSHKKGAKLAALQGSITS
  AHQVPAVSSVSHTALYTPPPEIPTTVLNIPHPSVISSPLLKSLHSAGPPLLAVSAAPPAQPLAKKKGVKR
  KADTTTPTPTAILAPGSPASPPGSLEPKAARLPPMRRESGRPIKPPRKDLPDSQQQHQSSKKGKLSEQLK
  HCNGILKELLSKKHAAYAWPFYKPVDASALGLHDYHDIIKHPMDLSTVKRKMENRDYRDAQEFAADVRLM
  FSNCYKYNPPDHDVVAMARKLQDVFEFRYAKMPDEPLEPGPLPVSTALPPGLAKSSSESSSEESSSESSS
  EEEEEEDEEDEEEESESSDSEEERAHRLAELQEQLRAVHEQLAALSQGPISKPKRKREKKEKKKKRKAEK
  HRGRIGIDEDDKGPRAPRPLQPKKSKKAGGGGSNATTLSHPGFGTSAGSSNKLPKKAQKTAPPVLPTGYD
  SEEEEESRPMSYDEKRQLSLDINKLPGEKLGRVVHIIQAREPSLRDSNPEEIEIDFETLKPSTLRELERY
  VLSCLRKKPRKPYTIRKPVGKTKEELALEKKRELEKRLQDVSGQLNSTKKPPKKASEKTESSAQQVAVSR
  LSASSSSSDSSSSSSSSSSSDTSDSDSG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_212495
• ORF Size: 2394 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_212495.1, NP_997660.1
• RefSeq Size: 3623 bp
• RefSeq ORF: 2397 bp
• Locus ID: 294276
• UniProt ID: Q6MGA9
• Cytogenetics: 20p12
• MW: 88.1 kDa
• Gene Summary: mouse homolog may act as a transcriptional regulator, possibly involved in regulation of mammary epithelial cell proliferation and lactation [RGD, Feb 2006]