R3hdm1 (NM_001134867) Rat Tagged ORF Clone

CAT#: RR210991

R3hdm1 (Myc-DDK-tagged ORF) - Rat R3H domain containing 1 (R3hdm1), (10 ug)

Product Images

Circular map for RR210991

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: R3hdm1
• Synonyms: R3hdm
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210991 representing NM_001134867
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGATGTCTGATATTGTTATTATAAAAGATGAAACTGAAACAATGAAGGATTTGGAGGCAGAAATGA
  GAGATACAACCAGAGTTGAAAATCTTATCAAATCAGAAAACTATGGGAAGATTTCGGCAGAGAAGAATGA
  ACATTGTATTGACAACAATATTGATTTGCAGCGGCCCCCGCAGTCATTTGGACAGACAGGAAAAAGGTCT
  AAGTCAAGCTCAAAGTTAAAGCTAGTTCGGAGCCTTGCAGTGTGTGAAGAATCTCCACCACCCCCTGCAG
  CAGAGATACCCCAGGAGACCCAGGAGAAAATTCAGATACAGTTAACACAATCATTTGAAAAAGAGGAAAA
  GCCCTCAAAAGATGAAACAGATAAAGAAAAAGCCAGTGATAAGTTACCCAGAAAAATGCTATCAAGAGAT
  TCCAGTCAAGAATACACTGATTCAACTGGCATAGATCTACATGAATTTTTAGTAAATACATTAAAAAACA
  ATCCCAGGGACAGAATGATGCTGCTGAAATTGGAACAAGAAATTTTAGATTTCATTGGTAATAATGAGTC
  CCCACGTAAGAAGTTTCCCCCGATGACATCTTACCACAGGATGCTGTTACACAGAGTGGCTGCCTACTTT
  GGATTAGACCACAATGTTGACCAGAGTGGGAAGTCTGTCATAGTGAACAAAACTAGCAACACCAGAATAC
  CTGATCAGAAATTTAATGAGCATATTAAGGATGACAGAGGTGAAGACTTTCAGAAACGTTACATCCTTAA
  GAGAGATAACTCTAGCTTTGACAAAGACGATAGCCAGATGAGAATACGTTTAAAAGATGACAGGAGAAGC
  AAATCCATAGAAGAAAGAGAAGAAGAGTACCAGAGAGCTAGAGACAGGATATTCTCCCAAGACTCCCTGT
  GTTCCCAAGAAAACTACATTATTGACAAAAGAATTCCAGATGAGGACACTATTGGTACCCAGCAGAGGCG
  CCAGATATTTAGAGTTAATAAAGATGCTTCAGGGCGATCAACAAACAGCCATCAGAGCAGCACTGAAAAT
  GAGTTGAAATACTCAGAGCCACGACCCTGGAGCAGCACAGATTCAGACAGCTCCCTCCGAAACCTGAAAC
  CCGCTGTAACCAAAGCCAGCAGCTTTAGCGGAATCTCGGTCCTGACAAGAGGTGATAGTTCTGGAAGCAG
  CAAAAGCATAGGCAGGCTTTCAAAAACAGGATCTGAGTCTTCAGGTAGTGTAGGGTCCTCTACAGGCTCT
  CTCTCTCACACCCAGCAGCCTCTGCCAGGCTCAGCTCTTAGCCAGTCTTCTCATGGCGCACCTGTCATCT
  ACCCGGCAGCCAGCAATCACAGTTCTCTTTCCTTTGATGGGGGCCTCAATGGTCAAGGTGCATCTCCTAG
  CACTAGCTTCCTTTTGCTTCCCTTGGAAGCGACTGGCATACCACCTGGCAGTATTCTGATCAACCCACAA
  ACAGGTCAGCCCTTCATAAACCCAGATGGAAGTCCAGTTGTGTACAATCCTCCCATGACTCAACAGTCAG
  TTCGAACCCAAGTGCCTGGTCCTCCACAGCCACCTCTGCCACCCCCACCACCTCAGCAGCCAGCAGCCAG
  CCACATGTTCTCACAGCCTGTTGGTCCTCTGCAGTCCTCTTCTCAGCCTGTTGAGTGCTCTCCAGCCCCC
  TACCCATCCCCGCTCCTGCCAGTCTCACCCACCCAGCAGTACTCTGTGGATAACCTTGGCTCTCAGTTCA
  GCCACATGAGTCTTGCCCGCCAGCCATCAGCCGATGGTTCTGACCCTCATGCCACCATGTTCCAATCCAC
  TGTTGTTCTTCAGTCTCCGCAGCAGTCTGGTTACATCGTGACGACAGCACCCCCACATCCTCCACCTCCT
  CCTCCACCACCTCCCCCTCCTCCTTCCCTACCACCTGGGCAGTCAGTCCCCACTGCCAGTTTCTCTGGGC
  ATCCTGTCAGCCAGCCTGTGCTCCAGCAGCAGGGGTTTCTTCCTCAGCCCTCACCACAGATGCCAGCCTG
  TTACTGTGCTCCAGGCCACTATCACTCCAGCCAACCTCAGTACCGCCCCATCCCTTCTGTCCATCACAGT
  TCACACCTAAACCAACCACTGCCACAGCCTGCACAGCACACAGGTTATCAAGTTATGCCCAACCAGCAGC
  AAAACTACCAAGGAATAGTTGGAGTTCAGTCACCGCAGAGTCAGAGCCTCATGGGAGGCCAGCCAAACAG
  CACTGGACCTCATATCCAAGGAGTAGTCATCCCCTACCCCTCAGTGCCATCGTATCAGGTTTCACTGCCT
  CAAGGTTCTCAAGGAATTGCCCATCAGACTTACCAACAGCCTGTTGTGTTCCCTAATCAGTCTAATCAAG
  GATCTCTGCCCACAGCAGGAATGCCAGTCTACTACAGTGTCATTCCACCTGGCCAACAAAGTAATTTAAG
  CTCTGCAGTCGGTTACTTACAACATCCTGGGTCAGAACAAGTACAGTTTCCTCGAACCACATCACCATGC
  AGCTCTCAACAGCTCCAAGGCCACCAGTGTGCAGCTTTGCCACAGCAGCCACCTGGTGGAGGAATGGTGA
  TGATGCAGCTCAACCTACCAAACAACGCACCGTCCCGAGCGCACTCACCCCCACAGTGGAAGCAAAACAA
  GTACCACTGTGACCATCAGAGAGGGCAGAAGTGTATGGAAGTGAGCCACATGGACAGTATTGTCCAGCAC
  AGCCCGCAGCTCAGCAGCCCCATCCTTTCTCCTGTGCAGTCACCAGCACCAGCGCAGCTGTCCGCTCTAA
  AGACCACCCGTCCCTCCGGGCCGCCGCTTTCCATCGTGTCCCAGTTCGCTAGACCTTTTGTTCCTGGGCA
  GGGAGATGCCAGGTATCCGCTGCTTGGCCAGCCCCTGCAGTACAGCCCTCCTACTCTTCTGCATGGACAC
  ATTCCACATCAACAGGGTCAGTCTGGCAGCAGGCATGGAAACCGAGGACGTAGACAAGCTAAGAAAGCTG
  CATCCACAGACCTTGGAGCAGGAGAAGCAGTCGTCGGCAAGGTCTTGGAGATCACAGAACTACCAGACGG
  AATAACTCGAATGGAAGCTGAGAAGCTTTTTGGGGAACTCTTTAAAATTGGCGCCAAGATCCGATGGCTC
  CGGGACCCCCAGTCTCAGCCCCAGCTACGTCGTCATCCTCTTTGCTGTGGTAGTGGGGATAACACTGTCA
  ACCCCGAGCGCTCTAAACCCAGTGACCTGGCCTCTACATACACTGTCCTAGCCACGTTCCCCTCCATTTC
  AGCTGCACAGAGTGCACTGAAGAAGCAGATCCATTCAGTGAACAAGTTTAAGCTGAGAATGAGCAAGAAG
  CACTACGACTTCCACATTTTGGAAAGGGCAAGTTCTCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210991 representing NM_001134867
  Red=Cloning site Green=Tags(s)
  
  MRMSDIVIIKDETETMKDLEAEMRDTTRVENLIKSENYGKISAEKNEHCIDNNIDLQRPPQSFGQTGKRS
  KSSSKLKLVRSLAVCEESPPPPAAEIPQETQEKIQIQLTQSFEKEEKPSKDETDKEKASDKLPRKMLSRD
  SSQEYTDSTGIDLHEFLVNTLKNNPRDRMMLLKLEQEILDFIGNNESPRKKFPPMTSYHRMLLHRVAAYF
  GLDHNVDQSGKSVIVNKTSNTRIPDQKFNEHIKDDRGEDFQKRYILKRDNSSFDKDDSQMRIRLKDDRRS
  KSIEEREEEYQRARDRIFSQDSLCSQENYIIDKRIPDEDTIGTQQRRQIFRVNKDASGRSTNSHQSSTEN
  ELKYSEPRPWSSTDSDSSLRNLKPAVTKASSFSGISVLTRGDSSGSSKSIGRLSKTGSESSGSVGSSTGS
  LSHTQQPLPGSALSQSSHGAPVIYPAASNHSSLSFDGGLNGQGASPSTSFLLLPLEATGIPPGSILINPQ
  TGQPFINPDGSPVVYNPPMTQQSVRTQVPGPPQPPLPPPPPQQPAASHMFSQPVGPLQSSSQPVECSPAP
  YPSPLLPVSPTQQYSVDNLGSQFSHMSLARQPSADGSDPHATMFQSTVVLQSPQQSGYIVTTAPPHPPPP
  PPPPPPPPSLPPGQSVPTASFSGHPVSQPVLQQQGFLPQPSPQMPACYCAPGHYHSSQPQYRPIPSVHHS
  SHLNQPLPQPAQHTGYQVMPNQQQNYQGIVGVQSPQSQSLMGGQPNSTGPHIQGVVIPYPSVPSYQVSLP
  QGSQGIAHQTYQQPVVFPNQSNQGSLPTAGMPVYYSVIPPGQQSNLSSAVGYLQHPGSEQVQFPRTTSPC
  SSQQLQGHQCAALPQQPPGGGMVMMQLNLPNNAPSRAHSPPQWKQNKYHCDHQRGQKCMEVSHMDSIVQH
  SPQLSSPILSPVQSPAPAQLSALKTTRPSGPPLSIVSQFARPFVPGQGDARYPLLGQPLQYSPPTLLHGH
  IPHQQGQSGSRHGNRGRRQAKKAASTDLGAGEAVVGKVLEITELPDGITRMEAEKLFGELFKIGAKIRWL
  RDPQSQPQLRRHPLCCGSGDNTVNPERSKPSDLASTYTVLATFPSISAAQSALKKQIHSVNKFKLRMSKK
  HYDFHILERASSQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001134867
• ORF Size: 3399 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001134867.1, NP_001128339.1
• RefSeq Size: 4817 bp
• RefSeq ORF: 3402 bp
• Locus ID: 304763
• Cytogenetics: 13q13
• MW: 124.1 kDa