Uqcrfs1 (NM_001008888) Rat Tagged ORF Clone

CAT#: RR210838

Uqcrfs1 (Myc-DDK-tagged ORF) - Rat ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 (Uqcrfs1), (10 ug)

Product Images

Circular map for RR210838

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Uqcrfs1
• Synonyms: LRRGT00195
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210838 representing NM_001008888
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGTCGGTCGCTGCCCGCTCGGGCCCGTTCGCGCCCGTCCTATCGGCCACGTCCCGCGGGGTGGCGG
  GAGCGCTGCGGCCCCTGCTGCAAAGCGCGGTGCCCGCCACCTCGGAACCACCGGTTCTGGACGTGAAGCG
  ACCCTTCCTGTGCCGTGAGTCGCTGAGTGGCCAGGCCGCGACCCGTCCTTTGGTGGCCACAGTGGGCCTG
  AATGTTCCTGCTTCTGTTCGTTATTCCCACACAGACATCAAGGTGCCCGACTTCTCTGACTATCGCCGTG
  CTGAAGTTCTAGATAGCACAAAATCTTCTAAAGAGAGCAGTGAGGCTAGAAAAGGCTTCTCTTACTTGGT
  AACTGCAACTACTACTGTGGGTGTTGCATACGCTGCGAAAAATGCAGTCTCCCAGTTTGTTTCCAGCATG
  AGTGCTTCTGCGGATGTACTGGCCATGTCGAAGATCGAGATCAAGTTGTCTGATATTCCAGAGGGGAAGA
  ACATGGCTTTTAAATGGAGAGGCAAACCTCTGTTTGTGCGCCATAGAACCAAGAAGGAGATTGACCAGGA
  AGCTGCAGTTGAAGTGTCCCAGTTAAGGGACCCACAGCATGATTTAGAGCGTGTAAAGAAGCCTGAATGG
  GTTATTCTGATCGGTGTCTGTACTCATCTTGGTTGTGTACCCATTGCAAACGCAGGAGATTTTGGTGGCT
  ACTATTGCCCCTGCCATGGGTCACACTATGATGCTTCTGGCAGGATCAGGAAGGGCCCTGCACCTCTCAA
  CCTGGAAGTACCTACCTATGAGTTCACCAGTGGTGATGTAGTTGTTGTGGGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210838 representing NM_001008888
  Red=Cloning site Green=Tags(s)
  
  MLSVAARSGPFAPVLSATSRGVAGALRPLLQSAVPATSEPPVLDVKRPFLCRESLSGQAATRPLVATVGL
  NVPASVRYSHTDIKVPDFSDYRRAEVLDSTKSSKESSEARKGFSYLVTATTTVGVAYAAKNAVSQFVSSM
  SASADVLAMSKIEIKLSDIPEGKNMAFKWRGKPLFVRHRTKKEIDQEAAVEVSQLRDPQHDLERVKKPEW
  VILIGVCTHLGCVPIANAGDFGGYYCPCHGSHYDASGRIRKGPAPLNLEVPTYEFTSGDVVVVG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001008888
• ORF Size: 822 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001008888.1, NP_001008888.1
• RefSeq Size: 1110 bp
• RefSeq ORF: 825 bp
• Locus ID: 291103
• UniProt ID: P20788
• Cytogenetics: 17p12
• MW: 29.4 kDa
• Gene Summary: component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome bc1 complex); important for generating an electrochemical potential coupled to ATP synthesis [RGD, Feb 2006]