Chrna3 (NM_052805) Rat Tagged ORF Clone
CAT#: RR210212
- TrueORF®
Chrna3 (Myc-DDK-tagged ORF) - Rat cholinergic receptor, nicotinic, alpha 3 (Chrna3), (10 ug)
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_052805" in other vectors (3)
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Chrna3 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR210212 representing NM_052805
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTGTTGTGCTGCTCCCGCCGCCGCTGTCCATGCTGATGCTGGTGCTGATGCTGCTGCCAGCGGCCA GTGCCTCAGAAGCTGAGCACCGCCTGTTCCAGTACCTGTTCGAAGATTACAACGAGATCATCCGGCCAGT GGCTAATGTGTCCCATCCAGTCATCATCCAGTTTGAGGTGTCCATGTCTCAGCTGGTGAAGGTGGATGAA GTAAACCAGATCATGGAAACCAACCTGTGGCTGAAGCAAATCTGGAATGACTACAAGCTGAAATGGAAAC CCTCTGACTACCAAGGGGTGGAGTTCATGCGTGTTCCTGCAGAGAAGATCTGGAAACCAGACATCGTACT GTACAACAACGCTGATGGGGATTTCCAGGTGGATGACAAGACCAAAGCTCTACTCAAGTACACAGGAGAA GTGACTTGGATCCCGCCGGCCATCTTTAAGAGCTCATGCAAAATCGACGTGACCTACTTCCCATTCGACT ACCAAAACTGCACCATGAAGTTCGGCTCCTGGTCCTACGACAAGGCAAAGATCGACCTGGTCCTCATCGG CTCCTCCATGAACCTCAAGGACTACTGGGAGAGTGGCGAGTGGGCTATCATTAAAGCCCCGGGCTACAAA CATGAAATCAAGTACAACTGCTGTGAGGAGATCTACCAAGACATCACGTACTCGCTGTACATCCGTCGCC TGCCGCTGTTCTACACCATCAACCTCATCATCCCCTGCCTGCTCATCTCCTTCCTCACTGTGCTTGTCTT CTACCTGCCCTCCGACTGTGGGGAGAAGGTGACACTCTGCATCTCTGTGCTCCTCTCCCTGACTGTCTTT CTCCTGGTGATCACCGAGACCATTCCTTCCACCTCGCTGGTCATCCCCCTGATTGGGGAGTACCTCCTCT TCACTATGATTTTTGTCACCTTGTCCATTGTCATCACAGTCTTTGTGCTCAATGTGCACTATAGAACTCC AACCACACACACCATGCCCACTTGGGTCAAGGCCGTGTTCTTGAACCTGCTCCCCAGGGTCATGTTTATG ACTAGGCCGACCAGTGGTGAGGGGGACACTCCTAAGACGAGGACCTTCTACGGCGCTGAGCTCTCAAACC TGAACTGCTTCAGCCGTGCAGACTCCAAAAGCTGCAAGGAAGGCTACCCCTGCCAAGATGGGACCTGTGG CTACTGCCACCACCGTAGGGTAAAAATCTCAAATTTCAGTGCCAACCTCACAAGAAGCTCCAGTTCTGAG TCTGTCGACGCTGTGTTGTCCCTCTCTGCCCTGTCACCAGAAATCAAAGAAGCCATCCAAAGTGTGAAGT ACATTGCCGAAAACATGAAAGCACAGAATGTAGCCAAAGAGATTCAAGATGATTGGAAGTACGTTGCCAT GGTGATTGATCGCATCTTTCTCTGGGTTTTCATCCTGGTGTGCATTTTAGGAACGGCGGGATTATTTCTG CAACCCTTGATGGCCAGAGATGACACA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR210212 representing NM_052805
Red=Cloning site Green=Tags(s) MGVVLLPPPLSMLMLVLMLLPAASASEAEHRLFQYLFEDYNEIIRPVANVSHPVIIQFEVSMSQLVKVDE VNQIMETNLWLKQIWNDYKLKWKPSDYQGVEFMRVPAEKIWKPDIVLYNNADGDFQVDDKTKALLKYTGE VTWIPPAIFKSSCKIDVTYFPFDYQNCTMKFGSWSYDKAKIDLVLIGSSMNLKDYWESGEWAIIKAPGYK HEIKYNCCEEIYQDITYSLYIRRLPLFYTINLIIPCLLISFLTVLVFYLPSDCGEKVTLCISVLLSLTVF LLVITETIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHYRTPTTHTMPTWVKAVFLNLLPRVMFM TRPTSGEGDTPKTRTFYGAELSNLNCFSRADSKSCKEGYPCQDGTCGYCHHRRVKISNFSANLTRSSSSE SVDAVLSLSALSPEIKEAIQSVKYIAENMKAQNVAKEIQDDWKYVAMVIDRIFLWVFILVCILGTAGLFL QPLMARDDT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_052805 |
ORF Size | 1497 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_052805.2, NP_434692.2 |
RefSeq Size | 1500 bp |
RefSeq ORF | 1500 bp |
Locus ID | 25101 |
Cytogenetics | 8q24 |
MW | 57 kDa |
Gene Summary | subunit of nicotinic acetylcholine receptor that is specific to the brain and peripheral nervous system [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN210212 | Chrna3 (untagged ORF) - Rat cholinergic receptor, nicotinic, alpha 3 (Chrna3), (10 ug) |
USD 503.00 |
|
RR210212L3 | Lenti ORF clone of Chrna3 (Myc-DDK-tagged ORF) - Rat cholinergic receptor, nicotinic, alpha 3 (Chrna3), (10 ug) |
USD 803.00 |
|
RR210212L4 | Lenti ORF clone of Chrna3 (mGFP-tagged ORF) - Rat cholinergic receptor, nicotinic, alpha 3 (Chrna3), (10 ug) |
USD 803.00 |
{0} Product Review(s)
Be the first one to submit a review