Zfp287 (NM_001107008) Rat Tagged ORF Clone

CAT#: RR210128

Zfp287 (Myc-DDK-tagged ORF) - Rat zinc finger protein 287 (Znf287), (10 ug)

Product Images

Circular map for RR210128

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Zfp287
• Synonyms: Znf287
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210128 representing NM_001107008
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTAGCCTCAAGAAAGAAGGTGGCAAATTCTTCACGCTCACAAGTCCTTCTAATGTGGAAGCCAGACA
  AGATTCAAAACGGACCCTGCAATGCTGAGAAGCAAATCCTCACCTCGAGACTCTTGCGTGACGCTGAAAC
  CTGTCGACAGAATTTTAGAAATTTTCCATACCCAGATGTGGCAGGTCCTCGGAAAGCATTGAGCCAGCTC
  CGAGAGCTCTGCCTGAAGTGGCTGAGACCTGAAGTTCATTCCAAGGAACAAATTCTGGAGTTGCTGGTGC
  TGGAGCAGTTTCTAAGCATCTTGCCTGGGGAGGTTAGGACTTGGGTAAATTCTCAGTACCCAGAGAACAG
  CGAGGAAGTGGTGGCTTTGGTGGAGGATCTGGCACAGATTCTAGAAGAGGAAGCTCCTCAGAATTCTGCC
  CTTCCCCAAGAAATCCCAGAGGAAGACCCCAAACATGCTCTCGAGACAGGGTGGCTCAATGACTTGGTGA
  CCAAAGAATCAGTGACATTCAATGACGTGGCTGTGGACATCACCCAAGAGGACTGGGAACTGATGCGCCC
  TGTTGAGAAGGAACTGTATAAGACTGTGACGTTACAGAACTATTGGAACATGGTTTCTCTGGGACTGACC
  GTGTACAGGCCAACTGTGATTCCCATCTTAGAAGAGCCATGGATGGTGATAAAAGAAATTGTAGAAGGCC
  CTAGTCCAGAATGGGAAACTAAAGCTCAAGCATGTAGTCCATTAAAGGATTTTCCTAAACTCAAACAAGA
  TGGAACCCAAACCATAAAACTGGAAGAATCCTGTGACTGTGAAGACGGCTTAGAGAGGCAGCCAGCGTAT
  GCCTTCAGGAAGATTCACATAGATGAAGATGACTTCAGTTTGAAGTCAGAGCTTTCACAAGAAGACCCTA
  CAGAAGAATGTCTTAGTAAATATGACCTATATAGAGATAATTTTGAAAAGCACACCAACCTAATTATACA
  GTTTGATACGCAGTCAGATAGTAAAACTTTACTGTATAATGAGAGCAAGCCAACATTCACTAATGTCTCA
  TCTGGTGGTGTTGTCCATGGGAAAATACTTCCCGGGGATAAGCCTTATTCCTGTAATGTCTGTGGAAAGA
  AATTTAGAAAATACCCATCACTCCTGGCACACCGAAATAACCATGCCAAAGACAAATCTTATGAATGTGA
  AGAATGTGGTAAAGAATTTAAGCATCTCTCATCCCTCATTGCACATCAGAGAATGCACACCGGAGAAAAA
  CCATACGAATGCCACCAGTGTGGGAAAGCCTTCAGTCAGCGTGCACACCTGACGATCCACCAGAGAATTC
  ATACTGGAGAAAAGCCCTACAAGTGTAACGACTGTGGGAAAGACTTCAGTCAGCGTGCACACCTCACTAT
  CCATCAGAGGACACACACTGGGGAGAAACCATATAAATGTTTGGAGTGCAGTAAAACCTTTAGCCATAGT
  TCATCCCTGATTAATCATCAGAGAGTTCATACTGGAGAAAAACCGTATATATGCAATGAGTGTGGGAAGA
  CTTTCAGTCAGAGTACGCACCTCCTCCAACATCAAAAAATTCACACTGGGAAAAAGCCGTATAAGTGCAA
  TGAGTGTTGGAAAGTGTTCAGCCAGAGCACTTACCTTATTCGCCATCAGCGAATCCATTCTGGAGAGAAG
  TGTTACAAATGCACTGAATGTGGGAAGGCCTTCGCCCACTCCTCAACCCTCATCCAACATCAGACCACTC
  ACACTGGAGAGAAATCCTATATCTGCAACGTGTGTGGGAAAGCCTTCAGCCAAAGCGCTAACCTTACCCA
  GCATCATAGAACACACACTGGAGAGAAGCCGTACAAATGTAGCGTGTGTGGGAAAGCCTTCAGCCAGAGT
  GTGCACCTCACTCAGCACCAGAGGATTCACAATGGAGAAAAGCCCTTCAAATGTAATACCTGCGGGAAAG
  CATACAGACAAGGTGCCAATCTGACCCAGCATCAACGAGTCCACACTGGAGAGAAGCCCTACAAGTGTCA
  CCACTGTGGGAAAGCCTTTATCTACTCCTCATCACTTAATCAACATCGAAGAACTCATACTGGAGAGAGA
  CCCTATAAGTGCACTCACTGTAACAAAGATTTTAGCCAGAGAACATGCCTTATTCAACACCAGAGGATTC
  ACACAGGAGAAAGGCCCTATGGATGCCGTATATGTGGGAAAAGCTTCACCCAGAGTACAAATCTTATTCA
  GCACCAACGGGTTCATATGGGCGCCAGACATCGCAATGGACAGATA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210128 representing NM_001107008
  Red=Cloning site Green=Tags(s)
  
  MLASRKKVANSSRSQVLLMWKPDKIQNGPCNAEKQILTSRLLRDAETCRQNFRNFPYPDVAGPRKALSQL
  RELCLKWLRPEVHSKEQILELLVLEQFLSILPGEVRTWVNSQYPENSEEVVALVEDLAQILEEEAPQNSA
  LPQEIPEEDPKHALETGWLNDLVTKESVTFNDVAVDITQEDWELMRPVEKELYKTVTLQNYWNMVSLGLT
  VYRPTVIPILEEPWMVIKEIVEGPSPEWETKAQACSPLKDFPKLKQDGTQTIKLEESCDCEDGLERQPAY
  AFRKIHIDEDDFSLKSELSQEDPTEECLSKYDLYRDNFEKHTNLIIQFDTQSDSKTLLYNESKPTFTNVS
  SGGVVHGKILPGDKPYSCNVCGKKFRKYPSLLAHRNNHAKDKSYECEECGKEFKHLSSLIAHQRMHTGEK
  PYECHQCGKAFSQRAHLTIHQRIHTGEKPYKCNDCGKDFSQRAHLTIHQRTHTGEKPYKCLECSKTFSHS
  SSLINHQRVHTGEKPYICNECGKTFSQSTHLLQHQKIHTGKKPYKCNECWKVFSQSTYLIRHQRIHSGEK
  CYKCTECGKAFAHSSTLIQHQTTHTGEKSYICNVCGKAFSQSANLTQHHRTHTGEKPYKCSVCGKAFSQS
  VHLTQHQRIHNGEKPFKCNTCGKAYRQGANLTQHQRVHTGEKPYKCHHCGKAFIYSSSLNQHRRTHTGER
  PYKCTHCNKDFSQRTCLIQHQRIHTGERPYGCRICGKSFTQSTNLIQHQRVHMGARHRNGQI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001107008
• ORF Size: 2286 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001107008.2, NP_001100478.1
• RefSeq Size: 5339 bp
• RefSeq ORF: 2289 bp
• Locus ID: 303212
• Cytogenetics: 10q23
• MW: 87.7 kDa