Adamts15 (NM_001106810) Rat Tagged ORF Clone

CAT#: RR210112

Adamts15 (Myc-DDK-tagged ORF) - Rat ADAM metallopeptidase with thrombospondin type 1 motif, 15 (Adamts15), (10 ug)

Product Images

Circular map for RR210112

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Adamts15
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210112 representing NM_001106810
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTCTACTGGGCATCTCAATCCTGGCTCTTGCCTGGCGACCTGCTAGCAGCTCCGAGCCAGAGTGGG
  AGGTGGTCGTTCCAATCCGACGGGACCCGGACATCAATGGCCGCCACTACTACAGGAGGGGTACGGAGGA
  CTCCGGGGATCAGGGTCTCATTTTTCAGATCACAGCTTTTCAGCAGGACTTTTATCTACACCTGACGCCA
  GATGCCCAGTTCCTGGCTCCCGCCTTTGCTACTGAGTATCTAGGTGTCCCCCTGCAGAGGCTCACTGGCA
  GCTCTCTAGACCTGCGACGCTGCTTCTATTCCGGGTATGTGAACGCGGAGCCAGACTCCTTTGCTGCTGT
  AAGCCTATGCGGGGGTCTCCGCGGAGCCTTTGGCTACCAAGGTGCGGAGTATGTCATTAGCCCTCTGCCC
  AACACCAGCGCGCCTGAGGCGCAGCGTCATAGCCAGGGCGCACACCTTCTCCAGCGCCGGGGTGCTCCCG
  TAGGGCCTTCCGGAGACCCTACCTCTCGCTGCGGGGTGGCCTCGGGCTGGAACCCCGCCATCCTGAGGGC
  CTTGGACCCTTATAAACCACGGCGGACGGGCGTGGGCGAAAGCCACAACCGGCGCAGGTCTGGGCGCGCC
  AAGCGCTTCGTGTCTATACCACGGTACGTGGAGACACTGGTGGTGGCGGACGAGTCAATGGTCAAGTTTC
  ACGGCGCGGATTTGGAACATTATCTGCTGACGCTGCTGGCCACGGCGGCGCGACTCTACCGCCACCCCAG
  CATCCTCAACCCTATCAACATCGTTGTGGTCAAGGTGTTACTCTTAGGAGATCGTGACACTGGGCCCAAG
  GTCACAGGCAACGCGGCCCTGACTCTGCGCAACTTCTGTGCCTGGCAGAAAAAGTTGAACAAAGTGAGCG
  ACAAGCACCCCGAGTACTGGGACACAGCCATCCTCTTCACCAGACAGGACCTATGCGGGGCTACCACCTG
  TGACACCTTGGGCATGGCTGATGTGGGCACCATGTGTGATCCCAAGAGAAGCTGCTCTGTCATCGAGGAC
  GATGGGCTTCCGTCGGCCTTCACCACTGCCCATGAGCTGGGCCATGTGTTCAACATGCCCCATGACAACG
  TGAAGGTGTGTGAGGAGGTGTTTGGGAAGCTCAGAGCCAACCACATGATGTCTCCGACACTCATCCAGAT
  CGACCGTGCCAACCCCTGGTCAGCCTGCAGTGCTGCCATTATCACCGACTTCCTGGACAGCGGGCACGGT
  GACTGCCTCCTGGACCAGCCCAGCAAGCCCATCACCCTGCCTGAGGACCTGCCAGGCACAAGCTACAGTT
  TGAGCCAACAGTGCGAGCTGGCCTTTGGGGTGGGCTCTAAGCCCTGCCCATATATGCAGTACTGTACAAA
  GCTGTGGTGCACCGGCAAGGCCAAGGGGCAGATGGTGTGCCAGACTCGCCACTTCCCCTGGGCAGATGGC
  ACCAGCTGTGGTGAGGGCAAGTTCTGCCTCAAGGGAGCCTGCGTGGAGAGACACAACCCAAACAAGTACC
  GGGTGGACGGCTCTTGGGCCAAGTGGGAGCCTTATGGTCCCTGCTCGCGCACCTGCGGTGGGGGCGTGCA
  GCTGGCCCGGAGGCAGTGCAGCAACCCTACCCCTGCCAACGGCGGGAAGTACTGCGAGGGAGTGAGAGTG
  AAATACCGATCTTGCAACTTGGAGCCCTGCCCCAGCTCAGCCTCTGGCAAGAGCTTCCGGGAAGAGCAGT
  GTGAGGCTTTCAATGGCTACAACCACAGTACCAACAGGCTCACTTTAGCTGTGGCATGGGTACCCAAGTA
  CTCCGGCGTGTCACCACGTGACAAGTGTAAGCTCATCTGCCGAGCCAATGGTACTGGCTACTTCTATGTG
  CTAGCACCTAAGGTGGTGGATGGTACGCTGTGTACTCCCGACTCCACCTCGGTCTGTGTCCAAGGCAAGT
  GCATCAAGGCTGGCTGTGATGGGAACCTGGGCTCCAAGAAGAAATTTGACAAATGTGGCGTGTGTGGTGG
  AGACAATAAGAGCTGCAAGAGGGTGACAGGACTCTTCACCAAGCCTATGCACGGCTACAATTTTGTAGTG
  GCCATCCCTGCAGGCGCCTCCAGCATTGACATCCGCCAGCGTGGTTACAAGGGGCTCATTGGGGATGACA
  ACTATCTGGCCCTGAAGAACAGCCAAGGCAAATACCTGCTCAATGGGCACTTTGTGGTATCTGCTGTAGA
  GAGGGACCTGGTGGTAAAGGGCAGTGTGCTACGCTATAGTGGCACTGGCACTGCGGTGGAGAGCCTGCAG
  GCGTCTCGACCCATCCTGGAGCCACTAACCGTGGAGGTCCTGTCCGTGGGGAAGATGACACCGCCCCGTG
  TACGTTATTCCTTCTATCTGCCCAAAGAGCCTCGGGAGGACAAATCCTCTCGCCCCAAGGACCCTCGGGG
  TCCCCCAGTGTTGCGCAACAGTGTTCTCAGCCTTTCCAACCAAGTAGAGCAGCCAGACAACAGGCCTCCT
  GCGCGCTGGGTGGCAGGCAACTGGGGGCCTTGCTCGGTGAGCTGTGGCAGTGGCCTACAGAAGCGAGCAG
  TGGACTGCCGGGACTCCCCAGGGCAGCAAGGAATCTCTGCTTGTGATGTAGACCATCGGCCAGTGGAGAA
  GCGAGCCTGTGGTGAGCCCTGCCCAACCTGGGAGCTCGGCAACTGGTCGCCCTGTTCTAAGAGCTGTGGC
  CGTGGATTTAAGAGGCGTCCGCTCAAGTGTGTGGGCCATGGAGGCAGGCTGCTGGCTCGGGACCAGTGTG
  ACCTACGCCGCAAGCCCCAGGAATTAGACTTCTGTGTCTTGAGACCCTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210112 representing NM_001106810
  Red=Cloning site Green=Tags(s)
  
  MLLLGISILALAWRPASSSEPEWEVVVPIRRDPDINGRHYYRRGTEDSGDQGLIFQITAFQQDFYLHLTP
  DAQFLAPAFATEYLGVPLQRLTGSSLDLRRCFYSGYVNAEPDSFAAVSLCGGLRGAFGYQGAEYVISPLP
  NTSAPEAQRHSQGAHLLQRRGAPVGPSGDPTSRCGVASGWNPAILRALDPYKPRRTGVGESHNRRRSGRA
  KRFVSIPRYVETLVVADESMVKFHGADLEHYLLTLLATAARLYRHPSILNPINIVVVKVLLLGDRDTGPK
  VTGNAALTLRNFCAWQKKLNKVSDKHPEYWDTAILFTRQDLCGATTCDTLGMADVGTMCDPKRSCSVIED
  DGLPSAFTTAHELGHVFNMPHDNVKVCEEVFGKLRANHMMSPTLIQIDRANPWSACSAAIITDFLDSGHG
  DCLLDQPSKPITLPEDLPGTSYSLSQQCELAFGVGSKPCPYMQYCTKLWCTGKAKGQMVCQTRHFPWADG
  TSCGEGKFCLKGACVERHNPNKYRVDGSWAKWEPYGPCSRTCGGGVQLARRQCSNPTPANGGKYCEGVRV
  KYRSCNLEPCPSSASGKSFREEQCEAFNGYNHSTNRLTLAVAWVPKYSGVSPRDKCKLICRANGTGYFYV
  LAPKVVDGTLCTPDSTSVCVQGKCIKAGCDGNLGSKKKFDKCGVCGGDNKSCKRVTGLFTKPMHGYNFVV
  AIPAGASSIDIRQRGYKGLIGDDNYLALKNSQGKYLLNGHFVVSAVERDLVVKGSVLRYSGTGTAVESLQ
  ASRPILEPLTVEVLSVGKMTPPRVRYSFYLPKEPREDKSSRPKDPRGPPVLRNSVLSLSNQVEQPDNRPP
  ARWVAGNWGPCSVSCGSGLQKRAVDCRDSPGQQGISACDVDHRPVEKRACGEPCPTWELGNWSPCSKSCG
  RGFKRRPLKCVGHGGRLLARDQCDLRRKPQELDFCVLRPC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001106810
• ORF Size: 2850 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001106810.1, NP_001100280.1
• RefSeq Size: 6064 bp
• RefSeq ORF: 2853 bp
• Locus ID: 300474
• Cytogenetics: 8q13
• MW: 104 kDa