Nt5c3b (NM_001007723) Rat Tagged ORF Clone
CAT#: RR209755
- TrueORF®
Nt5c3b (Myc-DDK-tagged ORF) - Rat 5'-nucleotidase, cytosolic III-like (Nt5c3l), (10 ug)
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_001007723" in other vectors (3)
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Nt5c3b |
Synonyms | Nt5c3l |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR209755 representing NM_001007723
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGGAGGTGAGCAGCCTGATGAAGGCCACGGTCCTGATGCGGCAGCCCGGACGGGTGCAGGAAA TCGTGAGCGCCCTTCGCAGGGGCGGAGGAGACCGCCTGCAGGTGATTTCTGATTTTGACATGACCTTGAG CAGATTTGCTTACAATGGACAGCGATGCCCCTCTTCCCACAATATTCTGGATAACAGCAAAATTATCAGT GAGGATTGTCGCAAAGAGCTCACCGAGCTCTTTCACCACTATTATCCCATTGAGATCGACCCACACCGGA CCATCAAAGAGAAGCTGCCTCACATGGTGCAGTGGTGGAGCAAAGCCCACAGCCTCCTGTGCCAGCAGAG GATACAGAAGTTCCAGATAGCTCAGGTGGTCGGCGAGTCCACTGCGATGCTCAGGGAGGGCTATAAGATG TTCTTCGACACACTCTACCATAACAACATTCCTCTTTTCATCTTCTCTGCGGGCATTGGTGATATCCTGG AAGAAATTATCCGACAGATGAAGGTGTTCCACCCCAACATCCACATTGTGTCCAACTACATGGATTTCAG TGAGGATGGTTTCCTGAAAGGATTCAAGGGCCAGCTCATCCACACCTACAACAAGAACAGCTCCGTGTGT GAGAACTCCAGTTACTTCCAGCAACTGCGGAACAAAACCAACATCATCCTGCTTGGAGACTCCATAGGGG ACCTCACTATGGCGGATGGGGTCCCTGGAGTACAGAACATTCTGAAGATCGGCTTCCTGAATGACAAGGT GGAGGAGCGGCGAGAACGTTACATGGACTCCTATGACATTGTGTTGGAGAAGGACGAGACGCTGGACGTG GTCAATGGGCTGCTGCAGCACATCCTGCGCCAGGGGGACTGTGTGGAGCTACAGGGCTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR209755 representing NM_001007723
Red=Cloning site Green=Tags(s) MAEEVSSLMKATVLMRQPGRVQEIVSALRRGGGDRLQVISDFDMTLSRFAYNGQRCPSSHNILDNSKIIS EDCRKELTELFHHYYPIEIDPHRTIKEKLPHMVQWWSKAHSLLCQQRIQKFQIAQVVGESTAMLREGYKM FFDTLYHNNIPLFIFSAGIGDILEEIIRQMKVFHPNIHIVSNYMDFSEDGFLKGFKGQLIHTYNKNSSVC ENSSYFQQLRNKTNIILLGDSIGDLTMADGVPGVQNILKIGFLNDKVEERRERYMDSYDIVLEKDETLDV VNGLLQHILRQGDCVELQGS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001007723 |
ORF Size | 900 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001007723.2, NP_001007724.2 |
RefSeq Size | 1683 bp |
RefSeq ORF | 903 bp |
Locus ID | 360629 |
UniProt ID | Q6AYP7 |
Cytogenetics | 10q31 |
MW | 34.5 kDa |
Gene Summary | Specifically hydrolyzes 7-methylguanosine monophosphate (m(7)GMP) to 7-methylguanosine and inorganic phosphate. The specific activity for m(7)GMP may protect cells against undesired salvage of m(7)GMP and its incorporation into nucleic acids. Also has weak activity for CMP. UMP and purine nucleotides are poor substrates (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN209755 | Nt5c3b (untagged ORF) - Rat 5'-nucleotidase, cytosolic III-like (Nt5c3l), (10 ug) |
USD 330.00 |
|
RR209755L3 | Lenti ORF clone of Nt5c3b (Myc-DDK-tagged ORF) - Rat 5'-nucleotidase, cytosolic III-like (Nt5c3l), (10 ug) |
USD 630.00 |
|
RR209755L4 | Lenti ORF clone of Nt5c3b (mGFP-tagged ORF) - Rat 5'-nucleotidase, cytosolic III-like (Nt5c3l), (10 ug) |
USD 630.00 |
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