Iws1 (NM_001034918) Rat Tagged ORF Clone

CAT#: RR209659

Iws1 (Myc-DDK-tagged ORF) - Rat IWS1 homolog (S. cerevisiae) (Iws1), (10 ug)

Product Images

Circular map for RR209659

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Iws1
• Synonyms: RGD1304762
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209659 representing NM_001034918
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTCGGAGTACTACAGCGGTGACCAGTCAGATGATGGTGGTGCAACCCCAGTACAGGACGAGCGGG
  ATTCAGGGTCAGATGGTGAGGATGATGTAAACGAGCAGCACTCCGGATCCGACACTGGAAGTGTAGACCG
  CCATTCAGAGAATGAAACTAGTGATAGAGAAGATGGCTTGACCAAGATACATAATGGAACAGACTCTGAG
  AATGATGAGCCCTCCAATGTTCATGCCAGTGACTCTGAAAGTGAGGAGCTTCACAGGCCAAAAGACAGTG
  ACTCTGAATCCGAGGAACATGCAGAGTCTCCTGCCAGTGATTCTGAAAACGAAGCTGTCCACCAGCAGGG
  GAGTGACTCTGAAAAGGAAGAGCTTCTTAATGGGCATGCAAGTGACTCTGAAAAGGAAGAGGGTAGAAAG
  CATGCAGCCAGTGACTCTGAAACCGAGGACACTCTGCAGCCTCAGGGCAGCGAGTCTGACAGTGAAGATC
  CCCCAAGGCCACAGGCCAGTGATTCAGAAAGCGAGGAGCCTCCCAAACCTCGGATCAGTGATTCAGAAAG
  CGAGGAGCTCCCCAAACCTCGGATCAGTGACTCAGAAAGTGAGGATCCTCCAAGGCCCCAGGTCAGTGAC
  TCAGAAAGTGAGGAGCTCCCCAAACCCCGAGTCAGTGATTCAGAAAGTGAGGATCCCCCAAGGCCACAGG
  CTAGTGACTCGGAAAGTGAGGAGCTCCCCAAACCCCGAGTCAGCGACTCGGAAAGTGAGGACCCTCAGAA
  AGGACCTGCTAGTGATTCTGAAGCTGAGGATGCCTCCAGACATAAAGAAAAGCCAGAGTCAGAGGACAGT
  GATGGGGAGAATAAGAGGGAAGACTCTGAAGTGCAGAATGAGTCAGACGGCCATGCAGACAGAAAAGGAC
  TCCACAGCTCTGACAGCGAGGAGGAAGAACCCAAGAGGCAAAAGATTGACAGTGATGACGACGGAGAAAA
  GGAGGGGGATGAGAAAGTAGCAAAGCGGAAGGCTGCCGTGCTTTCTGATAGTGAGGACGAAGACAAAGCA
  TCAGCAGCAAAGAAGAGTCGAGTTATCTCTGATGCTGATGATTCTGATAGTGATGTTGTATCAGACAAAT
  CAGGCAAAAGAGAGAAGACGGTAGCATCTGATAGTGAAGAAGAAGTCGGGAAAGAAGAGTCTTCTGTAAA
  GAAGAGTGAAGAGAAGGATCTGTTTGGGAGTGATAGTGAATCAGGCAATGAAGAAGAAAATCTTATTGCA
  GATATATTTGGAGAATCTGGTGATGAAGAGGAAGAGGAATTTACAGGATTTAACCAAGAAGATTTGGAGG
  AAGAGAAAAATGAAACACAGCTAAAAGAAGCAGAAGATTCTGATTCCGACGATAACATAAAGAGAGGAAA
  GCATATGGACTTTCTGTCAGATTTTGAGATGATGTTACAACGAAAAAAGAGCATGTGTGGCAAACGCAGA
  CGAAATCGAGATGGGGGCACTTTTATTAGTGACGCAGATGATGTCGTGAGTGCCATGATTGTCAAGATGA
  ACGAAGCTGCTGAGGAAGACAGGCAATTGAATAATCAAAAAAAGCCTGCTCTGAAAAAATTAACCTTATT
  GCCTACTGTGGTTATGCACCTTAAAAAGCAGGACCTTAAAGAAACATTTATTGACAGTGGTGTGATGTCT
  GCCATTAAAGAGTGGCTCTCACCTCTACCAGATAGGAGTTTGCCTGCACTGAAGATTCGGGAGGAATTGT
  TAAAGATTCTGCAAGAGCTACCTAGTGTGAGCCAAGAGACCCTGAAGCACAGTGGGATTGGCCGAGCAGT
  GATGTATCTGTATAAACATCCTAAGGAATCAAGGTCCAACAAGGACATGGCAGGGAAACTAATCAATGAG
  TGGTCTCGGCCCATATTTGGTCTTACCTCAAACTATAAAGGAATGACAAGAGAAGAAAGGGAGCAGAGAG
  ATCTAGAACAAATGCCACAGCGACGAAGAATGAGCAGCACTGGTGGTCAGACACCACGAAGAGACCTGGA
  AAAGGTACTAACAGGAGAGGAAAAGGCTCTTAGACCTGGAGATCCTGGCTTCTGTGCCCGTGCGAGAGTC
  CCAATGCCATCAAACAAGGACTATGTTGTCAGGCCCAAGTGGAATGTAGAAATGGAGTCATCCAGGCCTG
  GTATTCTTAAAAAGGGTCTAAGTCGATTGGAAAAGCATAAGAGGCGGTTTGCAGAGCAGAAGAGGCTCAG
  CCAAATGCACCGTGCTGTCAAGTTCAGCATCGAAGGCAACAGAATGCCCCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209659 representing NM_001034918
  Red=Cloning site Green=Tags(s)
  
  MDSEYYSGDQSDDGGATPVQDERDSGSDGEDDVNEQHSGSDTGSVDRHSENETSDREDGLTKIHNGTDSE
  NDEPSNVHASDSESEELHRPKDSDSESEEHAESPASDSENEAVHQQGSDSEKEELLNGHASDSEKEEGRK
  HAASDSETEDTLQPQGSESDSEDPPRPQASDSESEEPPKPRISDSESEELPKPRISDSESEDPPRPQVSD
  SESEELPKPRVSDSESEDPPRPQASDSESEELPKPRVSDSESEDPQKGPASDSEAEDASRHKEKPESEDS
  DGENKREDSEVQNESDGHADRKGLHSSDSEEEEPKRQKIDSDDDGEKEGDEKVAKRKAAVLSDSEDEDKA
  SAAKKSRVISDADDSDSDVVSDKSGKREKTVASDSEEEVGKEESSVKKSEEKDLFGSDSESGNEEENLIA
  DIFGESGDEEEEEFTGFNQEDLEEEKNETQLKEAEDSDSDDNIKRGKHMDFLSDFEMMLQRKKSMCGKRR
  RNRDGGTFISDADDVVSAMIVKMNEAAEEDRQLNNQKKPALKKLTLLPTVVMHLKKQDLKETFIDSGVMS
  AIKEWLSPLPDRSLPALKIREELLKILQELPSVSQETLKHSGIGRAVMYLYKHPKESRSNKDMAGKLINE
  WSRPIFGLTSNYKGMTREEREQRDLEQMPQRRRMSSTGGQTPRRDLEKVLTGEEKALRPGDPGFCARARV
  PMPSNKDYVVRPKWNVEMESSRPGILKKGLSRLEKHKRRFAEQKRLSQMHRAVKFSIEGNRMPL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001034918
• ORF Size: 2292 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001034918.1, NP_001030090.1
• RefSeq Size: 3010 bp
• RefSeq ORF: 2295 bp
• Locus ID: 291705
• UniProt ID: Q3SWT4
• Cytogenetics: 18p12
• MW: 85.4 kDa
• Gene Summary: Transcription factor which plays a key role in defining the composition of the RNA polymerase II (RNAPII) elongation complex and in modulating the production of mature mRNA transcripts. Acts as an assembly factor to recruit various factors to the RNAPII elongation complex and is recruited to the complex via binding to the transcription elongation factor SUPT6H bound to the C-terminal domain (CTD) of the RNAPII subunit RPB1 (POLR2A). The SUPT6H:IWS1:CTD complex recruits mRNA export factors (ALYREF/THOC4, EXOSC10) as well as histone modifying enzymes (such as SETD2) to ensure proper mRNA splicing, efficient mRNA export and elongation-coupled H3K36 methylation, a signature chromatin mark of active transcription (By similarity).[UniProtKB/Swiss-Prot Function]