Iws1 (NM_001034918) Rat Tagged ORF Clone
CAT#: RR209659
- TrueORF®
Iws1 (Myc-DDK-tagged ORF) - Rat IWS1 homolog (S. cerevisiae) (Iws1), (10 ug)
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_001034918" in other vectors (3)
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Iws1 |
Synonyms | RGD1304762 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR209659 representing NM_001034918
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTCGGAGTACTACAGCGGTGACCAGTCAGATGATGGTGGTGCAACCCCAGTACAGGACGAGCGGG ATTCAGGGTCAGATGGTGAGGATGATGTAAACGAGCAGCACTCCGGATCCGACACTGGAAGTGTAGACCG CCATTCAGAGAATGAAACTAGTGATAGAGAAGATGGCTTGACCAAGATACATAATGGAACAGACTCTGAG AATGATGAGCCCTCCAATGTTCATGCCAGTGACTCTGAAAGTGAGGAGCTTCACAGGCCAAAAGACAGTG ACTCTGAATCCGAGGAACATGCAGAGTCTCCTGCCAGTGATTCTGAAAACGAAGCTGTCCACCAGCAGGG GAGTGACTCTGAAAAGGAAGAGCTTCTTAATGGGCATGCAAGTGACTCTGAAAAGGAAGAGGGTAGAAAG CATGCAGCCAGTGACTCTGAAACCGAGGACACTCTGCAGCCTCAGGGCAGCGAGTCTGACAGTGAAGATC CCCCAAGGCCACAGGCCAGTGATTCAGAAAGCGAGGAGCCTCCCAAACCTCGGATCAGTGATTCAGAAAG CGAGGAGCTCCCCAAACCTCGGATCAGTGACTCAGAAAGTGAGGATCCTCCAAGGCCCCAGGTCAGTGAC TCAGAAAGTGAGGAGCTCCCCAAACCCCGAGTCAGTGATTCAGAAAGTGAGGATCCCCCAAGGCCACAGG CTAGTGACTCGGAAAGTGAGGAGCTCCCCAAACCCCGAGTCAGCGACTCGGAAAGTGAGGACCCTCAGAA AGGACCTGCTAGTGATTCTGAAGCTGAGGATGCCTCCAGACATAAAGAAAAGCCAGAGTCAGAGGACAGT GATGGGGAGAATAAGAGGGAAGACTCTGAAGTGCAGAATGAGTCAGACGGCCATGCAGACAGAAAAGGAC TCCACAGCTCTGACAGCGAGGAGGAAGAACCCAAGAGGCAAAAGATTGACAGTGATGACGACGGAGAAAA GGAGGGGGATGAGAAAGTAGCAAAGCGGAAGGCTGCCGTGCTTTCTGATAGTGAGGACGAAGACAAAGCA TCAGCAGCAAAGAAGAGTCGAGTTATCTCTGATGCTGATGATTCTGATAGTGATGTTGTATCAGACAAAT CAGGCAAAAGAGAGAAGACGGTAGCATCTGATAGTGAAGAAGAAGTCGGGAAAGAAGAGTCTTCTGTAAA GAAGAGTGAAGAGAAGGATCTGTTTGGGAGTGATAGTGAATCAGGCAATGAAGAAGAAAATCTTATTGCA GATATATTTGGAGAATCTGGTGATGAAGAGGAAGAGGAATTTACAGGATTTAACCAAGAAGATTTGGAGG AAGAGAAAAATGAAACACAGCTAAAAGAAGCAGAAGATTCTGATTCCGACGATAACATAAAGAGAGGAAA GCATATGGACTTTCTGTCAGATTTTGAGATGATGTTACAACGAAAAAAGAGCATGTGTGGCAAACGCAGA CGAAATCGAGATGGGGGCACTTTTATTAGTGACGCAGATGATGTCGTGAGTGCCATGATTGTCAAGATGA ACGAAGCTGCTGAGGAAGACAGGCAATTGAATAATCAAAAAAAGCCTGCTCTGAAAAAATTAACCTTATT GCCTACTGTGGTTATGCACCTTAAAAAGCAGGACCTTAAAGAAACATTTATTGACAGTGGTGTGATGTCT GCCATTAAAGAGTGGCTCTCACCTCTACCAGATAGGAGTTTGCCTGCACTGAAGATTCGGGAGGAATTGT TAAAGATTCTGCAAGAGCTACCTAGTGTGAGCCAAGAGACCCTGAAGCACAGTGGGATTGGCCGAGCAGT GATGTATCTGTATAAACATCCTAAGGAATCAAGGTCCAACAAGGACATGGCAGGGAAACTAATCAATGAG TGGTCTCGGCCCATATTTGGTCTTACCTCAAACTATAAAGGAATGACAAGAGAAGAAAGGGAGCAGAGAG ATCTAGAACAAATGCCACAGCGACGAAGAATGAGCAGCACTGGTGGTCAGACACCACGAAGAGACCTGGA AAAGGTACTAACAGGAGAGGAAAAGGCTCTTAGACCTGGAGATCCTGGCTTCTGTGCCCGTGCGAGAGTC CCAATGCCATCAAACAAGGACTATGTTGTCAGGCCCAAGTGGAATGTAGAAATGGAGTCATCCAGGCCTG GTATTCTTAAAAAGGGTCTAAGTCGATTGGAAAAGCATAAGAGGCGGTTTGCAGAGCAGAAGAGGCTCAG CCAAATGCACCGTGCTGTCAAGTTCAGCATCGAAGGCAACAGAATGCCCCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR209659 representing NM_001034918
Red=Cloning site Green=Tags(s) MDSEYYSGDQSDDGGATPVQDERDSGSDGEDDVNEQHSGSDTGSVDRHSENETSDREDGLTKIHNGTDSE NDEPSNVHASDSESEELHRPKDSDSESEEHAESPASDSENEAVHQQGSDSEKEELLNGHASDSEKEEGRK HAASDSETEDTLQPQGSESDSEDPPRPQASDSESEEPPKPRISDSESEELPKPRISDSESEDPPRPQVSD SESEELPKPRVSDSESEDPPRPQASDSESEELPKPRVSDSESEDPQKGPASDSEAEDASRHKEKPESEDS DGENKREDSEVQNESDGHADRKGLHSSDSEEEEPKRQKIDSDDDGEKEGDEKVAKRKAAVLSDSEDEDKA SAAKKSRVISDADDSDSDVVSDKSGKREKTVASDSEEEVGKEESSVKKSEEKDLFGSDSESGNEEENLIA DIFGESGDEEEEEFTGFNQEDLEEEKNETQLKEAEDSDSDDNIKRGKHMDFLSDFEMMLQRKKSMCGKRR RNRDGGTFISDADDVVSAMIVKMNEAAEEDRQLNNQKKPALKKLTLLPTVVMHLKKQDLKETFIDSGVMS AIKEWLSPLPDRSLPALKIREELLKILQELPSVSQETLKHSGIGRAVMYLYKHPKESRSNKDMAGKLINE WSRPIFGLTSNYKGMTREEREQRDLEQMPQRRRMSSTGGQTPRRDLEKVLTGEEKALRPGDPGFCARARV PMPSNKDYVVRPKWNVEMESSRPGILKKGLSRLEKHKRRFAEQKRLSQMHRAVKFSIEGNRMPL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001034918 |
ORF Size | 2292 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001034918.1, NP_001030090.1 |
RefSeq Size | 3010 bp |
RefSeq ORF | 2295 bp |
Locus ID | 291705 |
UniProt ID | Q3SWT4 |
Cytogenetics | 18p12 |
MW | 85.4 kDa |
Gene Summary | Transcription factor which plays a key role in defining the composition of the RNA polymerase II (RNAPII) elongation complex and in modulating the production of mature mRNA transcripts. Acts as an assembly factor to recruit various factors to the RNAPII elongation complex and is recruited to the complex via binding to the transcription elongation factor SUPT6H bound to the C-terminal domain (CTD) of the RNAPII subunit RPB1 (POLR2A). The SUPT6H:IWS1:CTD complex recruits mRNA export factors (ALYREF/THOC4, EXOSC10) as well as histone modifying enzymes (such as SETD2) to ensure proper mRNA splicing, efficient mRNA export and elongation-coupled H3K36 methylation, a signature chromatin mark of active transcription (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN209659 | Iws1 (untagged ORF) - Rat IWS1 homolog (S. cerevisiae) (Iws1), (10 ug) |
USD 770.00 |
|
RR209659L3 | Lenti ORF clone of Iws1 (Myc-DDK-tagged ORF) - Rat IWS1 homolog (S. cerevisiae) (Iws1), (10 ug) |
USD 1,069.00 |
|
RR209659L4 | Lenti ORF clone of Iws1 (mGFP-tagged ORF) - Rat IWS1 homolog (S. cerevisiae) (Iws1), (10 ug) |
USD 1,069.00 |
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