Eef1d (NM_001013104) Rat Tagged ORF Clone
CAT#: RR209228
- TrueORF®
Eef1d (Myc-DDK-tagged ORF) - Rat eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein) (Eef1d), (10 ug)
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_001013104" in other vectors (3)
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Eef1d |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR209228 representing NM_001013104
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGAGTGGGAAGGCCTCTTGTGCGCTGGAGACCGTCTGGGAGGACAAGCACAAGTATGAGGAAGCCG AGCGGCGCTTCCACGAGCATGAGGCCACACAAGCCGCCGCCGCCTCTGTCCAGCAGCTCCTGGCCGAAGT GCCAGCTGTGAACGGGCCCAGCAGCCAGGAGGACGCGGAGGACACCGACGAGGCAGAAACCCCCAACACC AGCAGCAGGAGTGATCCTAGGAAGAGCCACGAGTGCAAGAAGCCGTTACAGAAAAAGAGGAAGCGCTCCC CGAAGAGTTGGCTTGGCCAGGCGGACCTGGCCCTTGTGGGCCTCTCAGCTGACCACGTATGGTTGGACAA GCCACTCTTTGACCAGGCAGAAAGCTCCTACCGCCAGAGGCTGGCAGATGTAGCTGCCCAGGCAGCCCAG TCGCCTGCCCTGGCCCCTCGAGGGCCCTGTACACATGGAAGCCATGTGGCGTGCCACCATGTGACCTGGG GGATCTGGGTCAACAAGTCTTGCTTTGATCAAGCTGAGCGGGCTTTTGTGGAGTGGTCTCAGGCCTTGCT GCTGGCTGCAGAGGGGAGCCATAGGGAGGGGACTCCTGACACAGGCCAGCAGGCTGTCACTCCTGACCTG GCCTTGGCCTGCCAGCCTTGTCCACCAGCCAATGGCCAGCCTCCTCTGGGCAGCCTGCAGGCACTGGTGA GGGAGGTATGGCTGGAAAAGCCCCGTTATGATGCAGCTGAAAGGGGCTTCTATGAGGCCCTATTTGATGG CCACCCCCCAGGGAAAGTGCGCCTGCAGGAGCGAGCCAGTCAGGCGGAGGGTACTCGGAGGGGCCGCAGA GACCGTCGGAGCCGCAATACTGTAGGAAACAAGCGAGCTGGGTCAAAACGGGCCGATGGGGAGGCTCCTT CTGCCTTGCCCTACTGGTACTTCCTGCACAAGGACGCAGAGGCCCCCTGGCTTAGCAAGCCGACCTACGA CAGCGCCGAGTGTCGCCACCATGCCGCCGAGGCCCTGCGGATTGCCTGGCGCCTAGAAGCTGCCTCACTG GCTCACCGACCCACGCCCCGTTCTGGCCCATCCATGTCCAGCCTGAGACCCAAAAAAATGGCTACAAACT TTCTCATGCACGAGAAAATCTGGTTTGACAAGTTTAAATATGACGATGCAGAAAGGAGATTCTACGAGCA GATGAATGGGCCTGTGACCGCTGGCTCTCGGCAGGAGAATGGCGCCAGCGTGATCCTCCGAGACATTGCA AGAGCCAGAGAGAACATCCAGAAATCCTTGGCTGGAAGTTCAGGCCCTGGAGCCTCCAGTGGACCTGGTG GAGACCACAGTGACCTCATTGTGCGGATTGCCAGTCTGGAAGTAGAGAACCAGAACCTTCGAGGCGTGGT GCAAGATTTGCAGCAGGCCATTTCCAAGTTGGAGGTCCGGCTGAGCACTCTGGAGAAGAGTTCACCTACT CACCGAGCTACAGCCCCACAGACCCAACATGTCTCTCCTATGCGTCAAGTGGAGCCCCCAGCCAAGAAAG GAGCCACACCAGCAGAGGACGACGAGGACAATGACATTGACCTGTTCGGCAGTGATGAGGAAGAGGAAGA TAAGGAGGCTGCCCGACTACGGGAAGAGAGGCTACGCCAGTACGCAGAGAAGAAGGCCAAGAAGCCCACA CTGGTGGCCAAATCCTCCATCCTCTTGGATGTTAAACCTTGGGATGATGAGACAGACATGGCCCAGCTAG AGACTTGTGTGCGTTCCATCCAATTGGACGGACTGGTTTGGGGGGCCTCCAAGCTCGTGCCTGTCGGCTA TGGTATCCGGAAGCTGCAGATCCAGTGTGTGGTAGAGGATGACAAAGTGGGCACCGACTTGCTCGAAGAG GAGATCACCAAGTTTGAGGAGCATGTGCAGAGTGTCGACATTGCAGCCTTCAACAAGATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR209228 representing NM_001013104
Red=Cloning site Green=Tags(s) MRSGKASCALETVWEDKHKYEEAERRFHEHEATQAAAASVQQLLAEVPAVNGPSSQEDAEDTDEAETPNT SSRSDPRKSHECKKPLQKKRKRSPKSWLGQADLALVGLSADHVWLDKPLFDQAESSYRQRLADVAAQAAQ SPALAPRGPCTHGSHVACHHVTWGIWVNKSCFDQAERAFVEWSQALLLAAEGSHREGTPDTGQQAVTPDL ALACQPCPPANGQPPLGSLQALVREVWLEKPRYDAAERGFYEALFDGHPPGKVRLQERASQAEGTRRGRR DRRSRNTVGNKRAGSKRADGEAPSALPYWYFLHKDAEAPWLSKPTYDSAECRHHAAEALRIAWRLEAASL AHRPTPRSGPSMSSLRPKKMATNFLMHEKIWFDKFKYDDAERRFYEQMNGPVTAGSRQENGASVILRDIA RARENIQKSLAGSSGPGASSGPGGDHSDLIVRIASLEVENQNLRGVVQDLQQAISKLEVRLSTLEKSSPT HRATAPQTQHVSPMRQVEPPAKKGATPAEDDEDNDIDLFGSDEEEEDKEAARLREERLRQYAEKKAKKPT LVAKSSILLDVKPWDDETDMAQLETCVRSIQLDGLVWGASKLVPVGYGIRKLQIQCVVEDDKVGTDLLEE EITKFEEHVQSVDIAAFNKI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001013104 |
ORF Size | 1950 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001013104.1, NP_001013122.1 |
RefSeq Size | 2042 bp |
RefSeq ORF | 1953 bp |
Locus ID | 300033 |
UniProt ID | Q68FR9 |
Cytogenetics | 7q34 |
MW | 72.1 kDa |
Gene Summary | mouse homolog is a guanine nucleotide exchange protein that acts as a cadmium-responsive proto-oncogene [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN209228 | Eef1d (untagged ORF) - Rat eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein) (Eef1d), (10 ug) |
USD 665.00 |
|
RR209228L3 | Lenti ORF clone of Eef1d (Myc-DDK-tagged ORF) - Rat eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein) (Eef1d), (10 ug) |
USD 965.00 |
|
RR209228L4 | Lenti ORF clone of Eef1d (mGFP-tagged ORF) - Rat eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein) (Eef1d), (10 ug) |
USD 965.00 |
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