H2afy2 (NM_001135807) Rat Tagged ORF Clone

CAT#: RR209089

H2afy2 (Myc-DDK-tagged ORF) - Rat H2A histone family, member Y2 (H2afy2), (10 ug)

Product Images

Circular map for RR209089

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: H2afy2
• Synonyms: RGD1561371
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209089 representing NM_001135807
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGGCCGGAGCGGGAAGAAGAAAATGTCTAAGCTGTCTCGGTCAGCCAGGGCCGGGGTCATCTTTC
  CAGTAGGGCGGTTGATGCGCTACCTGAAGAAAGGAACTTTCAAGTACCGGATCAGCGTGGGTGCTCCAGT
  CTACATGGCTGCAGTCATTGAGTACCTGGCAGCGGAAATCCTAGAGTTGGCTGGGAACGCTGCGAGGGAC
  AACAAGAAGGCCCGGATAGCCCCCAGACACATCCTGCTGGCCGTTGCCAACGATGAGGAGCTCAACCAGT
  TGCTGAAAGGAGTGACCATCGCCAGTGGGGGTGTCTTGCCCAGAATTCACCCCGAACTGTTGGCCAAAAA
  GCGAGGGACCAAAGGCAAGTCAGAAACCATCCTCTCCCCACCCCCAGAGAAAAGAGGAAGGAAGGCCGCA
  TCAGGCAAGAAGGGGGGTAAGAAATCCAAGGCCACTAAGCCGCGGACGTCCAAGAAGTCCAAAGCCAAGG
  ACAGCGATAAAGAAGGAACATCGAATTCCACCTCAGAAGACGGGCCGGGGGATGGCTTTACCATCCTGTC
  TTCGAAGAGCCTCGTTCTGGGGCAGAAGCTGTCCCTGACCCAGAGTGACATCAGCCACATCGGCTCCATG
  AGGGTGGAGGGCATCGTCCACCCAACCACAGCCGAAATCGACCTCAAGGAAGAGATAGGTAAAGCCTTGG
  AAAAGGCCGGGGGTAAAGAGTTCTTGGAAACAGTAAAGGAGCTTCGCAAGTCCCAAGGCCCTTTGGAAGT
  TGCCGAAGCTGCCGTCAGCCAATCCAGTGGACTCGCAGCCAAGTTTGTCATCCACTGTCACATCCCCCAG
  TGGGGCTCCGACAAATGTGAAGAACAGCTGGAAGAGACCATCAAAAACTGCCTGTCCGCAGCCGAGGACA
  AGAAGCTTAAATCTGTCGCCTTCCCACCGTTCCCCAGTGGCAGAAACTGCTTTCCCAAACAGACGGCCGC
  CCAAGTGACCCTCAAGGCCATCTCGGCCCACTTCGACGACTCGAGCTCATCCTCGCTGAAGAATGTGTAC
  TTCCTGCTCTTCGACAGCGAGAGCATTGGCATCTACGTGCAGGAGATGGCCAAACTGGACACCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209089 representing NM_001135807
  Red=Cloning site Green=Tags(s)
  
  MSGRSGKKKMSKLSRSARAGVIFPVGRLMRYLKKGTFKYRISVGAPVYMAAVIEYLAAEILELAGNAARD
  NKKARIAPRHILLAVANDEELNQLLKGVTIASGGVLPRIHPELLAKKRGTKGKSETILSPPPEKRGRKAA
  SGKKGGKKSKATKPRTSKKSKAKDSDKEGTSNSTSEDGPGDGFTILSSKSLVLGQKLSLTQSDISHIGSM
  RVEGIVHPTTAEIDLKEEIGKALEKAGGKEFLETVKELRKSQGPLEVAEAAVSQSSGLAAKFVIHCHIPQ
  WGSDKCEEQLEETIKNCLSAAEDKKLKSVAFPPFPSGRNCFPKQTAAQVTLKAISAHFDDSSSSSLKNVY
  FLLFDSESIGIYVQEMAKLDTK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001135807
• ORF Size: 1116 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001135807.1, NP_001129279.1
• RefSeq Size: 1983 bp
• RefSeq ORF: 1119 bp
• Locus ID: 361844
• Cytogenetics: 20q11
• MW: 40.1 kDa