Rad54l (NM_001134960) Rat Tagged ORF Clone

CAT#: RR209078

Rad54l (Myc-DDK-tagged ORF) - Rat RAD54 like (S. cerevisiae) (Rad54l), (10 ug)

Product Images

Circular map for RR209078

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rad54l
• Synonyms: MGC189414
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209078 representing NM_001134960
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGAGGAGCTTAGCTCCCAGCCAATTGGCCAGGAGGAAACCAGAAGACATATCGTCTGATGATGAAG
  ACTGGCAGCCTGAGACAGTAACCCCTAAAAAACGCAAGTCCACTGCCGAGACCCAGGCCCAGCAGTGTTT
  CCTGTCTCCTTTTCGGAAGCCCTTGACTCAGTTACTCAACCGACCGCCTTGTCTGGATAGCAGTCAGCAT
  GAAGCGTTTATCCGAAGTATTTTGTCAAAGCCTTTCAAGGTCCCCATTCCAAATTATCAAGGTCCCCTGG
  GCTCTCGAGCATTGGGCCTGAAGAGGGCTGGCGTTCGTCGTGCCCTGCATGACCCTCTGGAAGAAGGTGC
  CTTGGTCCTGTATGAGCCTCCCCCACTGAGCGTCCATGACCAACTGAAGCTTGACAAAGAAAAACTCCCT
  GTTCACGTGGTTGTAGATCCTATTCTCAGTAAGGTGTTGCGACCTCATCAGAGAGAGGGAGTGAAGTTCC
  TTTGGGAGTGTGTCACCAGTCGACGAATTCCTGGAAGCCATGGCTGCATCATGGCTGATGAGATGGGCCT
  AGGAAAGACACTGCAGTGCATCACATTGATGTGGACACTTTTGCGCCAGAGCCCAGAGTGCAAGCCAGAA
  ATTGAGAAAGCAGTGGTGGTCTCACCTTCCAGCCTGGTGAAGAACTGGTACAATGAGGTTGAGAAATGGC
  TTGGAGGGAGGATCCAACCTCTGGCCATCGACGGAGGCTCAAAGGATGAGATAGACCGAAAACTGGAAGG
  ATTCATGAACCAGCGTGGAGCTAGGGTGCCTTCCCCCATCCTCATCATCTCCTATGAGACTTTCCGCCTG
  CATGTTGAAGTTCTTAAAAAAGGAAACGTTGGACTGGTCATATGTGACGAGGGCCACAGGCTAAAGAACT
  CTGAGAACCAGACTTACCAGGCTCTGGACAGTCTGAATACCAGCCGGCGGGTGCTCATCTCCGGGACCCC
  CATCCAGAACGATTTGCTTGAATATTTCAGCTTGGTGCACTTCGTTAATTCAGGCATTTTGGGAACTGCC
  CATGAATTCAAGAAGCATTTTGAATTACCAATTTTGAAGAGTCGAGATGCAGCTGCCAGTGAGGCAGATA
  GGCAGCTTGGAGAGGAACGTTTGCGGGAGCTCATCAGTATTGTGAACAGGTGCCTGATACGGAGAACATC
  TGATATCCTCTCTAAGTATCTGCCAGTGAAGATTGAGCAGGTGGTTTGTTGTAGGCTGACACCCCTTCAA
  ACTGAGCTATACAAGAGATTTCTAAGACAGGCTAAGCCTGAAGAAGAATTGCATGAGGGCAAGATGAGTG
  TATCTTCCCTGTCTTCTATCACCTCTCTAAAGAAGCTGTGTAATCATCCAGCTCTAATCTATGACAAGTG
  TGTGGCAGAGGAGGATGGCTTTGAGGGCACTTTGGGTATCTTCCCCCCTGGTTATAACTCTAAAGCTGTA
  GAACCACAGTTGTCGGGTAAGATGCTGGTCCTTGACTACATTCTGGCCATGACTCGAAGCCGTAGCAGTG
  ACAAAGTCGTGCTGGTGTCAAATTATACTCAGACATTGGATCTCTTTGAAAAGCTGTGCCGGGCTCGAAG
  GTACTTGTATGTTCGCCTGGATGGCACGATGTCCATTAAGAAGCGAGCCAAAGTTGTGGAGCGCTTCAAT
  AGCCCATCGAGCCCTGATTTCGTTTTCATGCTGAGCAGCAAAGCTGGAGGCTGTGGGCTTAACCTCATTG
  GTGCTAACCGGCTGGTCATGTTTGATCCTGACTGGAATCCAGCCAATGATGAACAAGCTATGGCCCGAGT
  CTGGCGAGACGGTCAAAAGAAGATCTGCTATATCTACCGACTGCTCTCTGCAGGAACAATTGAGGAAAAG
  ATCTTTCAGCGGCAAAGCCACAAGAAGGCTCTGAGCAGCTGTGTGGTAGATGAGGAGCAGGATGTGGAAC
  GTCACTTCTCTCTTGGTGAGCTCAAAGAGCTGTTTACCCTGGATGAAGCCAGCCTCAGTGACACTCACGA
  CAGACTGCATTGCCGCCGTTGTGTAAATAACCGCCAGGTCTGGCCACCCCCTGATGGTTCTGACTGCACT
  TCAGACCTGGCTCAGTGGAACCATAACACAGATAAACGAGGGCTCCAGGATGAGGTACTCCAGGCTGCCT
  GGGATGCTTCATCTACAGCCATCACCTTCGTCTTCCACCAGCGTTCCCATGAGGAGCAGCGGGGTCTTCA
  CTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209078 representing NM_001134960
  Red=Cloning site Green=Tags(s)
  
  MRRSLAPSQLARRKPEDISSDDEDWQPETVTPKKRKSTAETQAQQCFLSPFRKPLTQLLNRPPCLDSSQH
  EAFIRSILSKPFKVPIPNYQGPLGSRALGLKRAGVRRALHDPLEEGALVLYEPPPLSVHDQLKLDKEKLP
  VHVVVDPILSKVLRPHQREGVKFLWECVTSRRIPGSHGCIMADEMGLGKTLQCITLMWTLLRQSPECKPE
  IEKAVVVSPSSLVKNWYNEVEKWLGGRIQPLAIDGGSKDEIDRKLEGFMNQRGARVPSPILIISYETFRL
  HVEVLKKGNVGLVICDEGHRLKNSENQTYQALDSLNTSRRVLISGTPIQNDLLEYFSLVHFVNSGILGTA
  HEFKKHFELPILKSRDAAASEADRQLGEERLRELISIVNRCLIRRTSDILSKYLPVKIEQVVCCRLTPLQ
  TELYKRFLRQAKPEEELHEGKMSVSSLSSITSLKKLCNHPALIYDKCVAEEDGFEGTLGIFPPGYNSKAV
  EPQLSGKMLVLDYILAMTRSRSSDKVVLVSNYTQTLDLFEKLCRARRYLYVRLDGTMSIKKRAKVVERFN
  SPSSPDFVFMLSSKAGGCGLNLIGANRLVMFDPDWNPANDEQAMARVWRDGQKKICYIYRLLSAGTIEEK
  IFQRQSHKKALSSCVVDEEQDVERHFSLGELKELFTLDEASLSDTHDRLHCRRCVNNRQVWPPPDGSDCT
  SDLAQWNHNTDKRGLQDEVLQAAWDASSTAITFVFHQRSHEEQRGLHC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001134960
• ORF Size: 2244 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001134960.1, NP_001128432.1
• RefSeq Size: 2820 bp
• RefSeq ORF: 2247 bp
• Locus ID: 298429
• Cytogenetics: 5q35
• MW: 84.9 kDa