Tlr9 (NM_198131) Rat Tagged ORF Clone

CAT#: RR208760

Tlr9 (Myc-DDK-tagged ORF) - Rat toll-like receptor 9 (Tlr9), (10 ug)

Product Images

Circular map for RR208760

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tlr9
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR208760 representing NM_198131
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTCTCTGTAGCAGGACCCTGCACCCCTTGTCTCTCCTGGTACAGGCTGCAGTGCTGGCTGAGGCTC
  TGGCCCTGGGTACCCTGCCTGCCTTCCTACCCTGTGAACTGAAGCCTCATGGCCTGGTAGACTGCAACTG
  GCTGTTCCTGAAGTCTGTGCCTCACTTCTCTGCCGCAGAACCCCGTTCCAACATCACCAGCCTTTCCTTG
  ATCGCCAACCGCATCCACCACCTGCACAACCTCGACTTTGTCCACCTGCCCAACGTGCGACAGCTGAACC
  TCAAGTGGAACTGTCCGCCCCCTGGTCTCAGCCCCTTGCACTTCTCCTGCCGCATGACCATTGAGCCCAA
  AACCTTCCTGGCTATGCGCATGCTGGAAGAGCTGAACCTGAGCTATAACGGTATCACCACTGTGCCCCGC
  CTGCCCAGCTCCCTGACGAATCTGAGCCTAAGCCACACCAACATCCTGGTACTCGATGCCAGCAGCCTTG
  CTGGCCTGCACAGCCTGCGAGTTCTCTTCATGGACGGGAACTGCTACTACAAGAACCCCTGCAACGGGGC
  GGTGAACGTGACCCCGGACGCCTTCCTGGGCTTGAGCAACCTCACCCACTTGTCCCTTAAGTATAACAAC
  CTCACAGAGGTGCCCCGCCAACTGCCCCCCAGCCTGGAGTACCTCCTGCTGTCCTATAACCTCATCGTCA
  AGCTGGGGCCCGAAGACCTAGCCAACCTGACCTCCCTTCGAGTGCTTGATGTGGGTGGGAATTGCCGTCG
  CTGTGATCACGCCCCCGACCTCTGTACAGAATGCCGGCAGAAGTCCCTTGATCTGCACCCTCAGACTTTC
  CATCACCTGAGCCACCTTGAAGGCCTGGTGCTGAAGGACAGTTCTCTCCACTCGCTGAACTCCAAGTGGT
  TCCAGGGTCTGGTGAACCTCTCGGTGCTGGACCTAAGCGAGAACTTTCTCTACGAGAGCATCAACAAAAC
  CAGCGCCTTTCAGAACCTGACCCGTCTGCGCAAGCTCGACCTGTCCTTCAATTACTGCAAGAAGGTATCG
  TTCGCCCGCCTCCACCTGGCAAGTTCCTTCAAGAGCCTGGTGTCGCTGCAGGAGCTGAACATGAACGGCA
  TCTTCTTCCGCTTGCTCAACAAGAACACGCTCAGGTGGCTGGCTGGTCTGCCCAAGCTCCACACGCTGCA
  CCTTCAAATGAATTTCATCAACCAGGCGCAGCTCAGCGTCTTTAGTACCTTCCGAGCCCTTCGCTTTGTG
  GACCTGTCCAATAATCGCATCAGCGGGCCTCCAACGCTGTCCAGAGTCGCCCCCGAAAAGGCAGACGAGG
  CGGAGAAGGGGGTTCCATGGCCTGCAAGTCTCACCCCAGCTCTCCCGAGCACTCCCGTCTCAAAGAACTT
  CATGGTCAGGTGTAAGAACCTCAGATTCACCATGGACCTGTCTCGGAACAACCTGGTGACTATCAAGCCA
  GAGATGTTCGTCAACCTCTCCCATCTCCAGTGTCTGAGCCTGAGCCACAACTGCATCGCGCAGGCTGTCA
  ATGGCTCTCAGTTCCTGCCGCTGACCAACCTGAAGGTGCTGGACCTGTCCTATAACAAGCTGGACCTGTA
  CCATTCGAAATCGTTCAGTGAGCTCCCACAGTTGCAGGCCCTGGACCTGAGCTACAACAGCCAGCCCTTC
  AGCATGCAGGGGATAGGCCACAACTTCAGTTTTCTGGCCAATCTGTCCAGGTTACAGAACCTTAGCCTGG
  CACACAATGACATTCACAGCCGCGTGTCCTCACGCCTCTACAGCACCTCAGTGGAGTATCTGGACTTCAG
  CGGCAACGGTGTGGGCCGCATGTGGGACGAGGAGGACCTTTACCTCTATTTCTTCCAAGACCTGAGAAGC
  CTGATTCATCTGGACCTATCTCAGAATAAGCTGCACATCCTCCGGCCCCAGAACCTCAACTACCTCCCCA
  AGAGCCTGACGAAGCTGAGTTTCCGTGACAATCACCTCTCTTTCTTTAACTGGAGCAGTCTGGCCTTCCT
  GCCCAATCTGCGAGACCTGGACCTGGCAGGCAATCTACTAAAGGCCCTGACCAACGGCACCCTGCCTAAT
  GGCACGCTCCTCCAGAAACTGGATGTCAGTAGCAACAGTATCGTCTTTGTGGTCCCAGCCTTCTTTGCTC
  TGGCGGTAGAGCTAAAAGAGGTCAACCTCAGCCATAACATCCTCAAGACTGTGGATCGCTCCTGGTTTGG
  GCCCATTGTGATGAACCTGACGGTTCTAGACGTGAGCAGCAACCCTCTGCATTGTGCCTGCGGTGCACCC
  TTTGTAGACTTACTGCTGGAAGTGCAGACCAAGGTGCCTGGCCTGGCTAACGGTGTGAAGTGTGGCAGTC
  CCCGCCAGCTGCAGGGCCGCAGCATCTTTGCGCAAGACCTGCGGCTGTGCCTGGATGACGTCCTTTCTCG
  GGACTGCTTTGGCCTTTCACTCCTGGCTGTGGCCGTGGGCACGGTGTTGCCTTTACTGCAGCATCTCTGC
  GGCTGGGACGTCTGGTACTGTTTCCATCTGTGCCTGGCATGGCTACCTTTGCTGACCCGTGGCCGGCGCA
  GCGCCCAAGCTCTCCCTTATGATGCCTTCGTGGTGTTCGATAAGGCGCAGAGCGCGGTTGCTGACTGGGT
  GTATAACGAGCTTCGAGTGCGGCTAGAGGAGCGGCGCGGTCGCCGAGCCCTACGCTTGTGTCTGGAGGAC
  CGAGATTGGCTGCCTGGCCAGACACTCTTCGAGAACCTCTGGGCCTCCATCTATGGCAGCCGCAAGACTC
  TGTTTGTGCTGGCCCACACGGACAAGGTCAGTGGCCTCCTGCGCACCAGCTTCCTGCTGGCTCAGCAGCG
  CCTGCTGGAGGACCGCAAGGACGTGGTGGTGTTGGTGATCCTGCGCCCTGATGCCCACCGCTCCCGCTAC
  GTGCGACTGCGCCAGCGCCTCTGCCGCCAGAGTGTGCTCTTCTGGCCCCATCAGCCCAACGGGCAGGGCA
  GCTTCTGGGCCCAGCTGAGTACAGCCCTGACTAGGGACAACCACCACTTCTATAACCGGAACTTCTGCCG
  GGGACCTACAGCAGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR208760 representing NM_198131
  Red=Cloning site Green=Tags(s)
  
  MVLCSRTLHPLSLLVQAAVLAEALALGTLPAFLPCELKPHGLVDCNWLFLKSVPHFSAAEPRSNITSLSL
  IANRIHHLHNLDFVHLPNVRQLNLKWNCPPPGLSPLHFSCRMTIEPKTFLAMRMLEELNLSYNGITTVPR
  LPSSLTNLSLSHTNILVLDASSLAGLHSLRVLFMDGNCYYKNPCNGAVNVTPDAFLGLSNLTHLSLKYNN
  LTEVPRQLPPSLEYLLLSYNLIVKLGPEDLANLTSLRVLDVGGNCRRCDHAPDLCTECRQKSLDLHPQTF
  HHLSHLEGLVLKDSSLHSLNSKWFQGLVNLSVLDLSENFLYESINKTSAFQNLTRLRKLDLSFNYCKKVS
  FARLHLASSFKSLVSLQELNMNGIFFRLLNKNTLRWLAGLPKLHTLHLQMNFINQAQLSVFSTFRALRFV
  DLSNNRISGPPTLSRVAPEKADEAEKGVPWPASLTPALPSTPVSKNFMVRCKNLRFTMDLSRNNLVTIKP
  EMFVNLSHLQCLSLSHNCIAQAVNGSQFLPLTNLKVLDLSYNKLDLYHSKSFSELPQLQALDLSYNSQPF
  SMQGIGHNFSFLANLSRLQNLSLAHNDIHSRVSSRLYSTSVEYLDFSGNGVGRMWDEEDLYLYFFQDLRS
  LIHLDLSQNKLHILRPQNLNYLPKSLTKLSFRDNHLSFFNWSSLAFLPNLRDLDLAGNLLKALTNGTLPN
  GTLLQKLDVSSNSIVFVVPAFFALAVELKEVNLSHNILKTVDRSWFGPIVMNLTVLDVSSNPLHCACGAP
  FVDLLLEVQTKVPGLANGVKCGSPRQLQGRSIFAQDLRLCLDDVLSRDCFGLSLLAVAVGTVLPLLQHLC
  GWDVWYCFHLCLAWLPLLTRGRRSAQALPYDAFVVFDKAQSAVADWVYNELRVRLEERRGRRALRLCLED
  RDWLPGQTLFENLWASIYGSRKTLFVLAHTDKVSGLLRTSFLLAQQRLLEDRKDVVVLVILRPDAHRSRY
  VRLRQRLCRQSVLFWPHQPNGQGSFWAQLSTALTRDNHHFYNRNFCRGPTAE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198131
• ORF Size: 3096 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198131.1, NP_937764.1
• RefSeq Size: 3326 bp
• RefSeq ORF: 3099 bp
• Locus ID: 338457
• Cytogenetics: 8q32
• MW: 116.6 kDa