Stk11 (NM_001108069) Rat Tagged ORF Clone

CAT#: RR208420

Stk11 (Myc-DDK-tagged ORF) - Rat serine/threonine kinase 11 (Stk11), (10 ug)

Product Images

Circular map for RR208420

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Stk11
• Synonyms: Lkb1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR208420 representing NM_001108069
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGTGGCTGACCCCCAGCCGTTGGGCCTGTTCCCCGAGGGCGAGCTAATGTCGGTGGGCATGGACA
  CCTTCATCCACCGCATCGACTCCACCGAGGTGATCTACCAGCCGCGCCGCAAGCGCGCCAAGCTCATCGG
  CAAGTACCTGATGGGGGACCTGCTCGGGGAGGGCTCGTACGGCAAGGTGAAGGAGGTGCTGGACTCCGAG
  ACCTTATGCCGCAGGGCGGTCAAGATCCTCAAGAAGAAAAAGCTGCGCAGGATCCCCAATGGCGAGGCCA
  ACGTCAAGAAGGAGATCCAGCTGCTGCGGCGGCTGCGGCATCGGAATGTGATCCAGCTTGTGGATGTGCT
  GTACAATGAGGAGAAGCAGAAGATGTATATGGTGATGGAGTACTGCGTGTGTGGCATGCAGGAGATGCTG
  GACAGTGTGCCAGAGAAGCGCTTCCCCGTGTGCCAAGCTCATGGGTACTTCCGCCAGCTGATTGACGGCC
  TGGAGTACCTACACAGCCAGGGCATTGTTCACAAGGACATCAAGCCGGGCAACCTGCTCCTCACCACCAA
  TGGCACACTCAAGATCTCCGACCTCGGTGTTGCCGAGGCCTTGCACCCTTTCGCTGTGGATGACACCTGC
  CGGACCAGCCAGGGCTCCCCAGCCTTCCAGCCTCCAGAGATTGCCAATGGACTGGACACCTTTTCAGGTT
  TCAAGGTGGACATCTGGTCAGCTGGGGTCACACTCTACAACATCACCACGGGCCTGTACCCATTTGAGGG
  GGACAATATCTACAAGCTCTTTGAGAACATCGGGAGAGGGGACTTCACCATCCCTTGTGACTGCGCTCCA
  CCACTCTCTGACCTACTCCGAGGGATGTTGGAGTACGAGCCAGCCAAGAGGTTCTCCATCCGACAGATTA
  GACAGCACAGCTGGTTCCGGAAGAAACACCCGCTGGCCGAGGCTCTTGTGCCTATCCCCCCAAGTCCAGA
  CACTAAGGACCGCTGGCGCAGCATGACCGTAGTGCCCTACCTGGAGGACCTGCATGGCCGTGCAGAGGAG
  GAGGAGGACGAGGACTTGTTTGACATTGAGGACGGCATCATCTATACCCAGGACTTCACAGTGCCTGGAC
  AGGTCCTGGAAGAGGAAGTGGGTCAGAATGGACAGAGCCACAGCCTGCCCAAGGCTGTTTGTGTGAATGG
  CACAGAGCCCCAGCTCAGCAGCAAGGTGAAGCCAGAAGGCCGGCCTGGCGCTGCCAACCCTGCACGCAAG
  GTGTGCTCCAGCAACAAGATCCGCCGGCTCTCGGCCTGCAAGCAGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR208420 representing NM_001108069
  Red=Cloning site Green=Tags(s)
  
  MDVADPQPLGLFPEGELMSVGMDTFIHRIDSTEVIYQPRRKRAKLIGKYLMGDLLGEGSYGKVKEVLDSE
  TLCRRAVKILKKKKLRRIPNGEANVKKEIQLLRRLRHRNVIQLVDVLYNEEKQKMYMVMEYCVCGMQEML
  DSVPEKRFPVCQAHGYFRQLIDGLEYLHSQGIVHKDIKPGNLLLTTNGTLKISDLGVAEALHPFAVDDTC
  RTSQGSPAFQPPEIANGLDTFSGFKVDIWSAGVTLYNITTGLYPFEGDNIYKLFENIGRGDFTIPCDCAP
  PLSDLLRGMLEYEPAKRFSIRQIRQHSWFRKKHPLAEALVPIPPSPDTKDRWRSMTVVPYLEDLHGRAEE
  EEDEDLFDIEDGIIYTQDFTVPGQVLEEEVGQNGQSHSLPKAVCVNGTEPQLSSKVKPEGRPGAANPARK
  VCSSNKIRRLSACKQQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001108069
• ORF Size: 1308 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001108069.1, NP_001101539.1
• RefSeq Size: 3120 bp
• RefSeq ORF: 1311 bp
• Locus ID: 314621
• UniProt ID: A0A0H2UI02
• Cytogenetics: 7q11
• MW: 49.2 kDa
• Gene Summary: Tumor suppressor serine/threonine-protein kinase that controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. Acts by phosphorylating the T-loop of AMPK family proteins, thus promoting their activity: phosphorylates PRKAA1, PRKAA2, BRSK1, BRSK2, MARK1, MARK2, MARK3, MARK4, NUAK1, NUAK2, SIK1, SIK2, SIK3 and SNRK but not MELK. Also phosphorylates non-AMPK family proteins such as STRADA, PTEN and possibly p53/TP53. Acts as a key upstream regulator of AMPK by mediating phosphorylation and activation of AMPK catalytic subunits PRKAA1 and PRKAA2 and thereby regulates processes including: inhibition of signaling pathways that promote cell growth and proliferation when energy levels are low, glucose homeostasis in liver, activation of autophagy when cells undergo nutrient deprivation, and B-cell differentiation in the germinal center in response to DNA damage. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton. Required for cortical neuron polarization by mediating phosphorylation and activation of BRSK1 and BRSK2, leading to axon initiation and specification. Involved in DNA damage response: interacts with p53/TP53 and recruited to the CDKN1A/WAF1 promoter to participate in transcription activation. Able to phosphorylate p53/TP53; the relevance of such result in vivo is however unclear and phosphorylation may be indirect and mediated by downstream STK11/LKB1 kinase NUAK1. Also acts as a mediator of p53/TP53-dependent apoptosis via interaction with p53/TP53: translocates to the mitochondrion during apoptosis and regulates p53/TP53-dependent apoptosis pathways. Regulates UV radiation-induced DNA damage response mediated by CDKN1A. In association with NUAK1, phosphorylates CDKN1A in response to UV radiation and contributes to its degradation which is necessary for optimal DNA repair (By similarity).[UniProtKB/Swiss-Prot Function]