Wdr12 (NM_199410) Rat Tagged ORF Clone
CAT#: RR208407
- TrueORF®
Wdr12 (Myc-DDK-tagged ORF) - Rat WD repeat domain 12 (Wdr12), (10 ug)
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_199410" in other vectors (3)
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Wdr12 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR208407 representing NM_199410
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCACAACTCCAGGCACGTTTCTACACGGAGAACAAGAAATATGCAGTAGATGATGTTCCTTTCTCAA TCCCTGCCACCTCAGAAGTTGCTGACCTTAGTAATATTATCAATAAATTGCTGGAGACCAAAAATGAGCT CCACAAACATGTCGAGTTTGATTTCCTCATCAAGGGCCAGTTTCTTCGAATGCCCTTGGTCAAACACATG GAACTGGAAGACATTTCATCGGAAGAGGTTGTGGAACTAGAATATGTGGAAAAATACACCGCACCCCAGC CAGAGCAATGCATGTTCCACGATGACTGGATCAGCTCAATTAAAGGGGCAGAAGAATGGATCCTGTCTGG TTCTTATGATAAGACTTCTCGAATCTGGTCCTTGGAAGGAAAGTCAATAATGACAATTGTGGGACATACA GATGTTGTGAAAGATGTGGCTTGGGTGAAAAAAGACAGTCTGTCTTGCCTACTCCTGACGGCCTCGATGG ACCAGACTATTCTCTTATGGGAGTGGAACATAGAGAGGAACAAAGTGAAGGCTCTTCACTGCTGCAGAGG GCATGCTGGGAGTGTGGACGCCATAGCAGTTGATAGCTCTGGAACTAAATTCTGCAGTGGCTCCTGGGAT AAGATGCTGAAGATCTGGTCTACAGTCCCTACAGATGAGGAAGATGAAATACAAGAACCCACAAATCGAC CAAGAAAGAAGCAGAAAACAGAACAATTGGGACTGACAAGAACACCCTTGGTGACCCTCTCTGGCCACAC AGAAGCAATTTCCTCAGTACTCTGGTCAGATGCTGACGAAGTCTGCAGTGCCTCTTGGGATCACACAGTC AGAGTGTGGGATGTTGAGTCCGGAGGTCTTAAGTCAACTCTGACAGGAAATAAGGTGTTCAATTGTATTT CCTATTCTCCCCTCTGTAAACGTTTGGCGTCTGGAAGCACAGACAGGCACATTAGGCTGTGGGATCCCCG AACTAAAGATGGCTCTTTGGTGTCGCTGTCCCTCACCTCACACACAGGCTGGGTAACATCAGTGAAGTGG TCACCTACCCATGATCAGCAGCTGATCTCAGGCTCTTTAGATAACATGGTGAAGCTATGGGATACGAGAA GTTGTAAGGCTCCTCTCTACGACCTAGCTGCACATGAAGACAAAGTTCTAAGTGTGGACTGGACAGACAC AGGGCTACTTCTGAGTGGAGGAGCAGACAATAAATTGTATTCCTACAGATATTCCCCTACCACTTCCCAT GTGGGGGCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR208407 representing NM_199410
Red=Cloning site Green=Tags(s) MAQLQARFYTENKKYAVDDVPFSIPATSEVADLSNIINKLLETKNELHKHVEFDFLIKGQFLRMPLVKHM ELEDISSEEVVELEYVEKYTAPQPEQCMFHDDWISSIKGAEEWILSGSYDKTSRIWSLEGKSIMTIVGHT DVVKDVAWVKKDSLSCLLLTASMDQTILLWEWNIERNKVKALHCCRGHAGSVDAIAVDSSGTKFCSGSWD KMLKIWSTVPTDEEDEIQEPTNRPRKKQKTEQLGLTRTPLVTLSGHTEAISSVLWSDADEVCSASWDHTV RVWDVESGGLKSTLTGNKVFNCISYSPLCKRLASGSTDRHIRLWDPRTKDGSLVSLSLTSHTGWVTSVKW SPTHDQQLISGSLDNMVKLWDTRSCKAPLYDLAAHEDKVLSVDWTDTGLLLSGGADNKLYSYRYSPTTSH VGA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_199410 |
ORF Size | 1269 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_199410.2, NP_955442.1 |
RefSeq Size | 1490 bp |
RefSeq ORF | 1272 bp |
Locus ID | 363237 |
UniProt ID | P61480 |
Cytogenetics | 9q32 |
MW | 47.6 kDa |
Gene Summary | mouse homolog is a propeller-folded protein containing seven WD repeat units which appears to bind Notch1 and may be involved in Notch signalling activity [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN208407 | Wdr12 (untagged ORF) - Rat WD repeat domain 12 (Wdr12), (10 ug) |
USD 503.00 |
|
RR208407L3 | Lenti ORF clone of Wdr12 (Myc-DDK-tagged ORF) - Rat WD repeat domain 12 (Wdr12), (10 ug) |
USD 803.00 |
|
RR208407L4 | Lenti ORF clone of Wdr12 (mGFP-tagged ORF) - Rat WD repeat domain 12 (Wdr12), (10 ug) |
USD 803.00 |
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