Phospho2 (NM_001007642) Rat Tagged ORF Clone

CAT#: RR208366

Phospho2 (Myc-DDK-tagged ORF) - Rat phosphatase, orphan 2 (Phospho2), (10 ug)

Product Images

Circular map for RR208366

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Phospho2
• Synonyms: MGC93920
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR208366 representing NM_001007642
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAGTTCTGTTGGTGTTTGACTTTGACAATACAATCATAGACGACAACAGCGACACCTGGATTATAC
  AGTGTGCTCCAGACAAGAAGCTTCCCATTGAGCTACAAGATTCTTATCAGAAGGGGCTTTGGACAGAATT
  TATGGGCAGAGTCTTTAAGTATTTGAGAGATGAGGGGGTTAAAGAAGAGGAACTGAAAAGGGCAGTGACG
  TCACTACCCTTCACCTCAGGGATGATAGAACTTCTCAGCTTTCTGAGGATGAATAAGGACAGATTTGACT
  GCATCATCATTTCAGACTCAAATTCCATCTTCATAGATTGGGTTTTAGAAGCTGCTGCCTTTCATGATGT
  CTTTGATACCGTGTTTACCAATCCAGCGTCTTTTGATAGCACTGGTCGCCTCACAGTGAGAAATTGCCAT
  ACTCATGCTTGCACTAGGTGCCCAAAGAACCTTTGCAAAAATACAGTTTTGGGAGAGTTCATCGACAAAC
  AGTTACAAAAGGGAGTGAGGTACACGCGGATTGTTTATATCGGCGATGGCGGAAATGATGTCTGTCCAGT
  AACTTTTTTAAAGAAAAATGATGTTGCCATGCCGAGGGAAGGATATACTTTACATAGGACTCTTGACAAA
  ATGTCTCAGAATCTTGAGCCTATGGCATCTTCTATTGTAGTTTGGTCTTCAGGGATGGAAATAATTTCTC
  ATTTACAATTTCTAATACAGATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR208366 representing NM_001007642
  Red=Cloning site Green=Tags(s)
  
  MKVLLVFDFDNTIIDDNSDTWIIQCAPDKKLPIELQDSYQKGLWTEFMGRVFKYLRDEGVKEEELKRAVT
  SLPFTSGMIELLSFLRMNKDRFDCIIISDSNSIFIDWVLEAAAFHDVFDTVFTNPASFDSTGRLTVRNCH
  THACTRCPKNLCKNTVLGEFIDKQLQKGVRYTRIVYIGDGGNDVCPVTFLKKNDVAMPREGYTLHRTLDK
  MSQNLEPMASSIVVWSSGMEIISHLQFLIQM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001007642
• ORF Size: 723 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001007642.1, NP_001007643.1
• RefSeq Size: 2131 bp
• RefSeq ORF: 726 bp
• Locus ID: 295663
• UniProt ID: Q66HC4
• Cytogenetics: 3q21
• MW: 27.6 kDa
• Gene Summary: Phosphatase that has high activity toward pyridoxal 5'-phosphate (PLP). Also active at much lower level toward pyrophosphate, phosphoethanolamine (PEA), phosphocholine (PCho), phospho-l-tyrosine, fructose-6-phosphate, p-nitrophenyl phosphate, and h-glycerophosphate (By similarity).[UniProtKB/Swiss-Prot Function]