Usp36 (NM_001107069) Rat Tagged ORF Clone

CAT#: RR208196

Usp36 (Myc-DDK-tagged ORF) - Rat ubiquitin specific peptidase 36 (Usp36), (10 ug)

Product Images

Circular map for RR208196

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Usp36
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR208196 representing NM_001107069
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCATCGTGGATAAGCTGAAGGAGGCTCTAAAGCCTGGCCGCAAGGACTCGGCAGAGGATGGGGACC
  TGGGCAAGCTGCTGGCGGCCTCCGCCAAGAAGGTCCTCCTGCAGAGGATTGAGTTTGAACCGGCCAGCAA
  GAGCTTCTCTTACCAGCTGGAGTCCTTGAAGAGCAAATATGTACTGTTGAACCCCAAGGCTGAGGGTGCC
  GGGCGCCACAGGAGCGGAGATGAGCCACAGGCCAGGAAACCAGGCACCGAGCGCATGTCTGGAAGTGGAG
  GCGATGGAGTCCCTGCCCCACAGAAAGTGCTCTTCCCTGTGGAGCGACTGTCCTTGAGGTGGGAACGCGT
  ATTCCGAGTTGGTGCTGGGCTGCACAACCTGGGTAACACCTGCTTCCTGAACTCCACCATTCAGTGCTTG
  ACTTACACGCCACCTCTGGCCAACTACTTGCTCTCCAAGGAGCACGCACGGAGCTGTCACCAAGGAAGCT
  TCTGCATGCTGTGCGTCATGCAGAACCACATGGTCCAGGCCTTTGCCAACAGTGGCAATGCCATCAAGCC
  TGTCTCCTTCATCAGAGACCTGAAAAAGATTGCCCGGCACTTCCGATTTGGGAACCAAGAGGATGCACAT
  GAATTCCTGCGGTACACCATCGACGCCATGCAGAAGGCCTGCTTGAATGGCTATGCTAAGTTGGATCGGC
  AGACACAGGCTACTACCCTGGTGCATCAGATCTTTGGAGGCTATCTCAGGTCCCGAGTGAAGTGTTCCGT
  GTGCAGAAGCGTCTCAGACACATACGACCCCTACTTGGACATAGCGCTGGAGATTCGGCAAGCTGCAAAT
  ATTGTGCGTGCTCTGGAACTTTTTGTGAAATCAGATGTCCTGAGTGGAGAGAACGCCTATATGTGTGCTA
  AGTGTAAGAAGAAGGTTCCAGCTAGCAAGCGCTTCAGCATCCATAGAACATCCAATGTCCTGACTCTGTC
  CCTCAAGCGCTTTGCCAACTTCAGTGGGGGGAAGATCACCAAGGATGTTGGCTATCCAGAGTTCCTGAAC
  ATCCGTCCATACATGTCGCAGAGCAGTGGAGACCCTGTCATGTATGGGCTCTATGCTGTTCTGGTGCACT
  CAGGCTACAGCTGCCACGCGGGGCACTACTACTGCTACGTGAAGGCCAGCAATGGACAGTGGTACCAGAT
  GAACGACTCCTTGGTCCATTCCAGCAATGTCAAGGTGGTTCTGAGCCAGCAGGCCTACGTGCTCTTCTAT
  CTGCGGATTCCAGGCTCTAAGAAAAGTCCTGAGGGCCCTGTCTCCAGGGTGGGTGCCACTCTTCCTAGCC
  GTCCCAAGGTCATTCCAGAGCACCCCAAGAAGAGCCCTGGCAATGGGGTTGTTTCCTCCCCACTGATGGC
  AAAGCGACAAGACTCTCTAATGATGAGAAAGTTGCAGTCCCCTGAGGAGGTTGGCATGCCTGTGTCCAGA
  AATGGCTCCATTTCTGGTCTGAAGTTACAGAATGGATGTGCTCCTGCAAAGTCACCTGCCGGGTCCCCCT
  CCCCGAGACTCGCTCCGACACCCACACATATGCCCACCATTCTGGACGAGCCTGGGGAGAAGGTGAAGAA
  GTCAGCCCCGCCACAGTCTCGCACCACGTCCCCAACTGCTTCTCAGGGCTTTCCTGGGACCGGCGAGTCC
  AGGAGCCAGCGGCCTGGCTCCTGGGCGAGCAGAGACAGCATCTTCTCTACCTCACCAAAGCTGGCCAAAG
  CCATTACTAACGGGCACAGGCTGAAGGGGGAAGGCAATGGTGTGGACCTGGAGAAGGGGGATTCCAGCAG
  CTCGAGCCCCGAGCACTCTGCCAGCAGTGACGCTGCCAAGGCCCCGCAGACCTCAGAGGGCAGAGCTGCA
  CATGTCTGTGATTCTCAGGGAACAAACTGCCCAGCCACTGGCCATTCAAAAGTGCTGTCGAACGGAGTCG
  ATGCCAAGATGGGGAAGTTGAAGTCCCCTGCCCTGAGCAGCACCACCACCGAGCCCACAAGCCTCATGTC
  GCCTCCACCAGCCAAAAAACTGGCCCTGTCAGCCAAGAAGGCCAGCACCCTGCGGAGGGCGACCGGCAAT
  GACATCGGTTCACCTTCCCCCTCAGCATTCTGCGATCTCACCTCCCCCATGAAAGCCACCCACCCCGTCG
  TTGCCTCCCCTGGGCCTGTCAGTAAAACCAGGGCTGCTGCCGCACCTGCTCCCCAACCGTCTCCCCACCC
  CCACTCTGCATCACTGTCCAGTAGTAGCGCCAAGCCCTTAGGGACATCAGAGCCACAGAGCTGCTGCTCC
  TCTGCCTGGACACCCCTGCCTCAGGTCAATGGACACTTCACGTCCCACTTACACCAACTGCCAGAGGCCA
  ATGAGGCCCTACACAGCCCCTCCAGAAAGAGGAAGAAGACTCCTAGTGGAGATCCCCAGAGACTGGGCAG
  AGACACACACCTCCCACAGTGCCTCGAGGGAGCACCTGCAACAGCACGCAGGAAGAAGAGGAAGAAGAAG
  AAGGACCTGGAGGGTGTGGGCGACACAGCTACCCAGCAGGAAAGCCAGTCTCAGGACCAGCCTTGGAGTC
  CCAGGGGCCGGAAGGAAGAGGGTACGCAGCTTCAGGTAAATGGCCATCAAGTGAGTCACATCCTGGACAG
  TTACCATGCGAGCAGCAGGAAAAGGAGGAAGAGAAAAAGATCAGAAGGGCTCAGCCAAGAAGCCACCGTG
  TGCCAGGACCTACTTCAGCATGGCTGCTCCCCTGCAGACCACTGGCTGGGACGGGCACCTCTTGTTACCT
  GGAACAGAGATCAAGAACCGGATGTGGTCCAGGAGTTGCTTCAGGACTCGTCTGATACGGCTTATGGGAA
  AAAAGTTTTGACCTGGGATGGTGAGCCTTCAGCCATCAGTCAGGACGCCATGAAAGACAGCAGACTGGCC
  CGGACCCAGACCGTGGTTGATGACTGGGATGAAGAGTTTGATCGAGGGAAGAAAAAAGGAGAGAACAGTG
  CTCCA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR208196 representing NM_001107069
  Red=Cloning site Green=Tags(s)
  
  MPIVDKLKEALKPGRKDSAEDGDLGKLLAASAKKVLLQRIEFEPASKSFSYQLESLKSKYVLLNPKAEGA
  GRHRSGDEPQARKPGTERMSGSGGDGVPAPQKVLFPVERLSLRWERVFRVGAGLHNLGNTCFLNSTIQCL
  TYTPPLANYLLSKEHARSCHQGSFCMLCVMQNHMVQAFANSGNAIKPVSFIRDLKKIARHFRFGNQEDAH
  EFLRYTIDAMQKACLNGYAKLDRQTQATTLVHQIFGGYLRSRVKCSVCRSVSDTYDPYLDIALEIRQAAN
  IVRALELFVKSDVLSGENAYMCAKCKKKVPASKRFSIHRTSNVLTLSLKRFANFSGGKITKDVGYPEFLN
  IRPYMSQSSGDPVMYGLYAVLVHSGYSCHAGHYYCYVKASNGQWYQMNDSLVHSSNVKVVLSQQAYVLFY
  LRIPGSKKSPEGPVSRVGATLPSRPKVIPEHPKKSPGNGVVSSPLMAKRQDSLMMRKLQSPEEVGMPVSR
  NGSISGLKLQNGCAPAKSPAGSPSPRLAPTPTHMPTILDEPGEKVKKSAPPQSRTTSPTASQGFPGTGES
  RSQRPGSWASRDSIFSTSPKLAKAITNGHRLKGEGNGVDLEKGDSSSSSPEHSASSDAAKAPQTSEGRAA
  HVCDSQGTNCPATGHSKVLSNGVDAKMGKLKSPALSSTTTEPTSLMSPPPAKKLALSAKKASTLRRATGN
  DIGSPSPSAFCDLTSPMKATHPVVASPGPVSKTRAAAAPAPQPSPHPHSASLSSSSAKPLGTSEPQSCCS
  SAWTPLPQVNGHFTSHLHQLPEANEALHSPSRKRKKTPSGDPQRLGRDTHLPQCLEGAPATARRKKRKKK
  KDLEGVGDTATQQESQSQDQPWSPRGRKEEGTQLQVNGHQVSHILDSYHASSRKRRKRKRSEGLSQEATV
  CQDLLQHGCSPADHWLGRAPLVTWNRDQEPDVVQELLQDSSDTAYGKKVLTWDGEPSAISQDAMKDSRLA
  RTQTVVDDWDEEFDRGKKKGENSAP
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001107069
• ORF Size: 3015 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001107069.1, NP_001100539.1
• RefSeq Size: 4263 bp
• RefSeq ORF: 3018 bp
• Locus ID: 303700
• Cytogenetics: 10q32.3
• MW: 109.1 kDa