Ibsp (NM_012587) Rat Tagged ORF Clone

CAT#: RR207755

Ibsp (Myc-DDK-tagged ORF) - Rat integrin binding sialoprotein (Ibsp), (10 ug)

Product Images

Circular map for RR207755

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ibsp
• Synonyms: Bsp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR207755 representing NM_012587
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGACTGCTTTAATCTTGCTCTGCATTTTGGGGATGGCGAGTGCTTTCTCGATGAAAAATTTCCATA
  GAAGAATCAAAGCAGAGGATTCTGAAGAAAACGGGGTCTTTAAGTACCGGCCACGCTACTTTCTTTATAA
  GCATGCCTACTTTTATCCTCCTCTGAAACGGTTTCCAGTCCAGGGAGGCAGCGACTCTTCGGAAGAAAAT
  GGAGATGGCGATAGTTCGGAGGAGGAGGGGGAGGAAGAGGAGACTTCAAATGAAGAGGAAAACAACGAAG
  ACTCTGAGGGGAATGAAGACCAGGAGGCGGAGGCAGAGAACGCCACACTCTCAGGGGTAACCGCTAGCTA
  CGGGGTAGAGACCACAGCTGACGCTGGAAAGTTGGAGTTAGCTGCGCTCCAGCTACCCAAGAAGGCTGGA
  GATGCAGAGGGCAAGGCTCCAAAAATGAAGGAAAGCGACGAGGAAGAGGAAGAGGAAGAGGAGGAAGAAA
  ACGAGAACGAAGAAGCAGAAGTGGATGAAAATGAGCAGGTCGTCAACGGCACCAGCACCAACTCCACGGA
  GGTGGACGGGGGGAATGGCCCCAGCGGAGGAGACAACGGAGAAGAAGCCGAGGAGGCAAGCGTCACTGAA
  GCAGGTGCAGAAGGAACAACAGCAGGGGCCAGGGAGCTGACCAGTTATGGCACCACGACAGCTGTCCTTC
  TGAACGGGTTTCAGCAGACGACCCCGCCACCCGAAGCCTATGGGACCACCTCCCCACCAGCCAGAAAGAG
  CAGCACGGTTGAGTATGGGGAAGAATACGAACAAATAGGCAACGAGTACAACACTGCGTATGAAACCTAT
  GACGAGAACAACGGGGAGCCTCGCGGGGACACTTACCGAGCTTATGAGGACGAATACAGCTACTACAAGG
  GGCATGGCTATGAAGGCTACGAGGGTCAGGATTATTACTACCACCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR207755 representing NM_012587
  Red=Cloning site Green=Tags(s)
  
  MKTALILLCILGMASAFSMKNFHRRIKAEDSEENGVFKYRPRYFLYKHAYFYPPLKRFPVQGGSDSSEEN
  GDGDSSEEEGEEEETSNEEENNEDSEGNEDQEAEAENATLSGVTASYGVETTADAGKLELAALQLPKKAG
  DAEGKAPKMKESDEEEEEEEEEENENEEAEVDENEQVVNGTSTNSTEVDGGNGPSGGDNGEEAEEASVTE
  AGAEGTTAGARELTSYGTTTAVLLNGFQQTTPPPEAYGTTSPPARKSSTVEYGEEYEQIGNEYNTAYETY
  DENNGEPRGDTYRAYEDEYSYYKGHGYEGYEGQDYYYHQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012587
• ORF Size: 957 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012587.2, NP_036719.2
• RefSeq Size: 1948 bp
• RefSeq ORF: 960 bp
• Locus ID: 24477
• Cytogenetics: 14p22
• MW: 35.1 kDa
• Gene Summary: extracellular matrix protein that binds hydroxyapatite; involved in bone formation [RGD, Feb 2006]