Ltbp1 (NM_021587) Rat Tagged ORF Clone

CAT#: RR207625

Ltbp1 (Myc-DDK-tagged ORF) - Rat latent transforming growth factor beta binding protein 1 (Ltbp1), (10 ug)

Product Images

Circular map for RR207625

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ltbp1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR207625 representing NM_021587
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGAGCCTGGCTCAGGTGGGGACTCCTGCTCTGGGCAGGGCTGCTAGCGTGGTCTGCGCACGGCC
  GGGTGCGGAGGATCACGTACGTGGTGCGCCCGGGCCCGGGACTGCCGGCCGGAACCCTGCCTCTGGCTGG
  GCCCCCGCGGACCTTCAACGTCGCACTGGACGCCAGGTACAGCCGGAGCTCAACAGCCACCTCCAGCCGC
  TCCTTGGCCGGGCCCCCAGCCGAGCGGACCCGGCGCACCAGCCAGCCTGGCGGCGCGGCCCTGCCCGGGC
  TCCGATCTCCGCTGCCACCCGAGCCCGCGCGTCCGGGGGCGCCCAGCCGGCAGCTCCACTCAAAGGCCGG
  TGCTCAGACCGCGGTCACTCGCTTCGCCAAACATGGCAGGCAAGTCGTGCGCTCCAAAGTGCAGCAGGAC
  ACGCAGAGCTCCGGGGGCTCCAGACTGCAGGTTCAGCAGAAGCAGCAGCTGCAGGGGATCAATGTCTGTG
  GAGGGCAGTGCTGCCATGGGTGGAGTAAGGCCCCGGGCTCCCAGAGGTGCACCAAACCAAGCTGTGTTCC
  ACCATGCCAGAATGGAGGGATGTGTCTCCGGCCTCAGTTCTGTGTGTGTAAGCCAGGGACAAAGGGCAAA
  GCCTGCGAGATCACAGCTGCCCAGGACACCATGTCACCAGTGTTTGGAGGGCAGAACCCTGGCTCCTCGT
  GGGTCCCTCCTGAGCCAGCAGCAAAGCGTACTTCAACCAAGAAGGCAGACACCTTACCAAGGGTCAGTCC
  AGTGGCCCAGATGACCTTGACCCTCAAGCCGAAGCCGTCCATGGGACTCTCCCAGCAGATACATTCTCAA
  GTGGCACCCTTGTCTTCCCAGAATGTGATGATTCGGCATGGCCAGACCCAGGAGTATGTGCTCAAGCCCA
  AGTACTTCCCAGCCCCAAAGGTGGTCTCTGGAGAGCAGTCCACAGAAGGCTCTTTCTCTTTAAGATATGG
  GCAGGAGCAAGGCACAGCACCCTTTCAGGTGAGTAACCACACTGGTCGCATCAAGGTGGTCTTTACTCCG
  AGCATCTGTAAAGTGACCTGCACCAAGGGCAACTGTCACAACAGCTGCCAGAAGGGGAATACCACCACTC
  TCATTAGTGAGAACGGCCATGCAGCCGACACCCTGACAGCCACGAACTTCCGAGTGGTAATTTGCCATCT
  TCCATGTATGAATGGTGGCCAGTGCAGTTCAAGGGACAAATGTCAGTGCCCTCCAAATTTCACAGGAAAG
  CTTTGCCAGATCCCTGTCCTTGGTGCCAGTATGCCTAAACTCTACCAGCACGCCCAGCAGCCAGGCAAGG
  CACTGGGGAGTCACGTCATCCATTCCACACATACCTTGCCTCTAACCATGACCAACCAGCAAGGGGTCAA
  AGTGAAATTTCCCCCCAACATAGTCAATATCCATGTGAAGCATCCTCCTGAGGCTTCTGTGCAGATACAC
  CAGGTTTCCAGAATTGATGGCCCAGTGGGCCAGAGGGTGAAAGAAGTGCAACCGGGCCAGTCCCAAGTCT
  CTTACCAAGGGCTGCCCGTCCAGAAGACCCAGACAGTCCATTCCACATACTCCCACCAGCAGGTCATTCC
  GCATGTGTATCCCGTGGCTGCTAAGACACAACTTGGACGATGCTTCCAGGAAACCATTGGGTCACAGTGT
  GGCAAAGCGCTCCCTGGCCTTTCAAAGCAAGAGGACTGCTGTGGGACAGTGGGGACCTCCTGGGGCTTTA
  ACAAATGCCAGAAATGCCCCAAGAAGCAATCCTATCATGGATATACTCAAATGATGGAATGCCTACAAGG
  ATATAAACGGGTTAACAACACCTTTTGTCAAGATATTAATGAATGTCAGCTCCAAGGCGTATGCCCTAAT
  GGGGAGTGTTTGAATACCATGGGCAGCTACAGATGCTCCTGCAAAATGGGATTTGGGCCTGACCCTACCT
  TTTCAAGTTGTGTGCCGGATCCCCCTATGATCTCAGAAGAGAAAGGGCCCTGCTACCGCCTCGTCAGTCC
  TGGGAGGCAGTGTATGCACCCTCTCTCTGTTCACCTCACCAAGCAGATCTGCTGTTGTAGTGTGGGCAAG
  GCCTGGGGCCCACAGTGTGAGAAATGTCCCCTCCCGGGCACAGCTGCTTTTAAGGAAATCTGTCCTGGTG
  GAATGGGTTATACGGTTTCTGGCATTCATAGACGCAGGCCAATCCATCAGCATATAGGTAAAGAAGCTGT
  ATTTGTCAAGCCAAAGAACACTCAACCTGTTGCTAAAAGTACTCATCCTCCACCTCTCCCAGCCAAGGAA
  GAGCCTGTGGAGGCCCTGACCTCCTCGCGGGAACATGGGCCGGGAGTGGCAGAGCCTGAAGTGGTAACAG
  CACCTCCTGAGAAGGAAATACCCTCACTGGATCAAGAGAAAACAAGACTTGAACCTGGGCAACCCCAGCT
  CTCCCCAGGGGTTTCAACTATTCATCTGCACCCACAGTTCCCAGTAGTGGTTGAAAAGACATCCCCTCCT
  GTGCCTGTGGAGGTGGCTCCTGAAGGTTCTACATCAAGTGCCAGCCAAGTGATTGCTCCTACGCAAGTAA
  CAGAAATCAACGAATGTACTGTGAACCCGGATATTTGCGGAGCAGGACACTGCATCAACCTGCCGGTGAG
  GTATACCTGCATATGCTACGAGGGCTACAAGTTCAGTGAGCAGCAGAGGAAGTGCATTGACATTGATGAA
  TGTGCTCAGGCCCAGCATCTCTGCTCCCAGGGTCGCTGTGAGAACACAGAGGGAAGTTTCCTGTGTATTT
  GCCCAGCAGGGTTTATAGCCAGTGAGGAGGGTTCTAACTGTATAGATGTGGATGAATGTCTGAGGCCCGA
  TGTGTGTAGGGACGGCCGCTGCATCAACACTGCTGGGGCCTTCCGATGCGAATACTGTGACAGTGGGTAC
  CGGATGTCACGACGGGGCCACTGTGAGGATATCGATGAGTGTCTGACCCCAAGTACCTGTCCCGAGGAAC
  AATGCGTGAATTCCCCAGGTTCTTACCAGTGTGTGCCCTGCACAGAAGGGTTCCGTGGCTGGAATGGACA
  ATGCCTCGATGTGGACGAGTGCCTGCAGCCAAAGGTCTGTACCAATGGTTCCTGCACCAACCTGGAAGGC
  TCCTACATGTGTTCCTGCCACAAGGGCTACAGCCCCACACCAGACCATAGACACTGTCAAGATATTGATG
  AATGTCAGCAAGGGAACCTGTGCATGAACGGGCAGTGCAAAAACACTGACGGCTCCTTCCGGTGTACCTG
  TGGGCAGGGCTATCAGCTGTCAGCGGCTAAAGACCAATGTGAAGATATTGACGAATGCGAGCACCGTCAC
  CTCTGCTCTCACGGGCAGTGCAGGAACACAGAGGGCTCCTTCCAGTGTTTGTGCAACCAGGGTTACAGAG
  CATCTGTGCTTGGAGACCACTGCGAGGATATCAATGAATGCTTGGAGGACAGTAGTGTCTGCCAGGGAGG
  TGACTGCATCAATACAGCAGGGTCCTATGACTGCACGTGCCCGGATGGACTCCAGCTGAATGACAATAAG
  GGCTGTCAAGACATTAATGAATGTGCACAGCCAGGACTCTGTGCACCTCATGGGGAGTGTCTAAACACAC
  AAGGCTCATTCCACTGTGTCTGTGAACAAGGGTTCTCCATCTCTGCAGATGGTCGTACTTGTGAAGATAT
  TGATGAGTGTGTTAACAACACTGTGTGTGACAGTCACGGCTTCTGTGACAACACAGCCGGCTCTTTCCGC
  TGCCTCTGTTATCAGGGCTTTCAAGCCCCACAGGATGGGCAAGGGTGTGTGGATGTGAACGAATGTGAAC
  TGCTCAGTGGTGTATGTGGGGAGGCTTTCTGTGAAAATGTGGAAGGGTCCTTCCTGTGCGTGTGTGCCGA
  TGAGAACCAGGAGTACAGCCCCATGACTGGGCAGTGTCGCTCCCGGGCTACTGAAGATTCAGGTGTGGAT
  CGTCAGCCCAAAGAAGAAAAGAAGGAGTGTTATTATAATCTCAATGATGCCAGTCTCTGTGATAACGTGC
  TGGCCCCCAACGTCACCAAACAAGAGTGCTGCTGTACATCGGGCGCCGGCTGGGGAGACAATTGTGAGAT
  CTTCCCTTGCCCAGTCCAGGGGACTGCTGAGTTCTCGGAAATGTGCCCTAGAGGAAAAGGTTTTGTCCCT
  GCTGGAGAATCCTCTTACGAAACCGGTGGTGAGAACTACAAAGATGCTGACGAATGCCTGCTGTTTGGAG
  AGGAAATCTGCAAAAACGGTTACTGTTTGAACACTCAGCCTGGGTATGAATGCTACTGCAAGGAAGGGAC
  ATACTACGATCCTGTCAAATTACAGTGTTTTGATATGGATGAATGCCAAGACCCTAACAGTTGTATCGAT
  GGCCAGTGTGTTAATACAGAGGGCTCTTACAACTGCTTTTGCACCCACCCAATGGTCCTGGATGCCTCTG
  AGAAGAGATGTGTGCAGCCAACTGAATCAAATGAACAAATAGAAGAAACCGATGTCTATCAAGATCTGTG
  CTGGGAGCATCTGAGTGAGGAGTACGTGTGTAGCCGTCCTCTTGTAGGCAAGCAGACGACATACACAGAG
  TGCTGCTGTTTGTACGGGGAGGCATGGGGCATGCAGTGTGCTCTCTGCCCCATGAAGGACTCAGATGACT
  ATGCCCAGCTGTGCAACATCCCTGTGACAGGACGGCGGCGACCATATGGACGGGATGCGTTGGTGGACTT
  CAGTGAACAGTATGGCCCAGAAACAGACCCTTACTTCATTCAGGATCGCTTTCTAAACAGCTTTGAGGAG
  CTACAGGCTGAGGAATGTGGCATCCTCAACGGCTGTGAAAATGGCCGCTGTGTAAGGGTTCAGGAAGGTT
  ATACTTGCGATTGCTTTGATGGATATCATCTGGATATGGCCAAGATGACCTGTGTTGATGTAAATGAATG
  CAGCGAGCTGAATAATCGGATGTCTCTCTGCAAGAACGCCAAGTGCATTAACACAGAAGGCTCCTACAAA
  TGCGTGTGTCTACCAGGCTACGTACCATCTGACAAGCCCAACTACTGTACACCACTGAACACCGCTTTGA
  ATTTAGACAAAGACAGTGACCTGGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR207625 representing NM_021587
  Red=Cloning site Green=Tags(s)
  
  MAGAWLRWGLLLWAGLLAWSAHGRVRRITYVVRPGPGLPAGTLPLAGPPRTFNVALDARYSRSSTATSSR
  SLAGPPAERTRRTSQPGGAALPGLRSPLPPEPARPGAPSRQLHSKAGAQTAVTRFAKHGRQVVRSKVQQD
  TQSSGGSRLQVQQKQQLQGINVCGGQCCHGWSKAPGSQRCTKPSCVPPCQNGGMCLRPQFCVCKPGTKGK
  ACEITAAQDTMSPVFGGQNPGSSWVPPEPAAKRTSTKKADTLPRVSPVAQMTLTLKPKPSMGLSQQIHSQ
  VAPLSSQNVMIRHGQTQEYVLKPKYFPAPKVVSGEQSTEGSFSLRYGQEQGTAPFQVSNHTGRIKVVFTP
  SICKVTCTKGNCHNSCQKGNTTTLISENGHAADTLTATNFRVVICHLPCMNGGQCSSRDKCQCPPNFTGK
  LCQIPVLGASMPKLYQHAQQPGKALGSHVIHSTHTLPLTMTNQQGVKVKFPPNIVNIHVKHPPEASVQIH
  QVSRIDGPVGQRVKEVQPGQSQVSYQGLPVQKTQTVHSTYSHQQVIPHVYPVAAKTQLGRCFQETIGSQC
  GKALPGLSKQEDCCGTVGTSWGFNKCQKCPKKQSYHGYTQMMECLQGYKRVNNTFCQDINECQLQGVCPN
  GECLNTMGSYRCSCKMGFGPDPTFSSCVPDPPMISEEKGPCYRLVSPGRQCMHPLSVHLTKQICCCSVGK
  AWGPQCEKCPLPGTAAFKEICPGGMGYTVSGIHRRRPIHQHIGKEAVFVKPKNTQPVAKSTHPPPLPAKE
  EPVEALTSSREHGPGVAEPEVVTAPPEKEIPSLDQEKTRLEPGQPQLSPGVSTIHLHPQFPVVVEKTSPP
  VPVEVAPEGSTSSASQVIAPTQVTEINECTVNPDICGAGHCINLPVRYTCICYEGYKFSEQQRKCIDIDE
  CAQAQHLCSQGRCENTEGSFLCICPAGFIASEEGSNCIDVDECLRPDVCRDGRCINTAGAFRCEYCDSGY
  RMSRRGHCEDIDECLTPSTCPEEQCVNSPGSYQCVPCTEGFRGWNGQCLDVDECLQPKVCTNGSCTNLEG
  SYMCSCHKGYSPTPDHRHCQDIDECQQGNLCMNGQCKNTDGSFRCTCGQGYQLSAAKDQCEDIDECEHRH
  LCSHGQCRNTEGSFQCLCNQGYRASVLGDHCEDINECLEDSSVCQGGDCINTAGSYDCTCPDGLQLNDNK
  GCQDINECAQPGLCAPHGECLNTQGSFHCVCEQGFSISADGRTCEDIDECVNNTVCDSHGFCDNTAGSFR
  CLCYQGFQAPQDGQGCVDVNECELLSGVCGEAFCENVEGSFLCVCADENQEYSPMTGQCRSRATEDSGVD
  RQPKEEKKECYYNLNDASLCDNVLAPNVTKQECCCTSGAGWGDNCEIFPCPVQGTAEFSEMCPRGKGFVP
  AGESSYETGGENYKDADECLLFGEEICKNGYCLNTQPGYECYCKEGTYYDPVKLQCFDMDECQDPNSCID
  GQCVNTEGSYNCFCTHPMVLDASEKRCVQPTESNEQIEETDVYQDLCWEHLSEEYVCSRPLVGKQTTYTE
  CCCLYGEAWGMQCALCPMKDSDDYAQLCNIPVTGRRRPYGRDALVDFSEQYGPETDPYFIQDRFLNSFEE
  LQAEECGILNGCENGRCVRVQEGYTCDCFDGYHLDMAKMTCVDVNECSELNNRMSLCKNAKCINTEGSYK
  CVCLPGYVPSDKPNYCTPLNTALNLDKDSDLE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021587
• ORF Size: 5136 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021587.1, NP_067598.1
• RefSeq Size: 6244 bp
• RefSeq ORF: 5139 bp
• Locus ID: 59107
• UniProt ID: Q00918
• Cytogenetics: 6q13
• MW: 186.6 kDa
• Gene Summary: large subunit component of masking protein; neutralizes the activity of TGF-beta 1 [RGD, Feb 2006]