Spata31a5 (NM_001008359) Rat Tagged ORF Clone

CAT#: RR207328

Spata31 (Myc-DDK-tagged ORF) - Rat family with sequence similarity 75, member A4 (Fam75a4), (10 ug)

Product Images

Circular map for RR207328

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Spata31a5
• Synonyms: Aep1; Fam75a4; Spata31
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR207328 representing NM_001008359
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAATCTTCCCTCTCTTCTGGAAAGCATTTACACCACATGGCTGAGCCTCAGCTCCACTATATGGG
  CAATGGACATGATCCTTGCCTTTGTGTGTGGACTGGGGCTCTACCTCCTGTTACTTCCTTTTCTTGAGAG
  TCATCTGTCTTCATCTCCATCAGACATAAATTTCACCAGGAAGCCTCAGATACAGATGACCTGGCAGAGC
  CAGTACATGAAAAAGTTTAGGAATCATTGTAGGAACGATGCAAAAGCTTGGGAAGAATGCCTGAAAAAGC
  TGAAAGAAAAAGAAAAGGATGAATTATTTCTAGAAGAAATGAGCCCAGGACACCATTTGAACTCTTTAGG
  GAATATGTTTAACTCAGCAAGTGCTAAACAAGACAGCACTACCTTGTCACCCTTCTGGAACCTGAAAGAA
  AAATCAGAAGAGCAGATGGCCACTCAGAAACTATCATATCCCAAGATCTCAGGGGACCATTTACAAGAGA
  AATATGACCAGCTTTACTGGGGTCTCCCTTCTCTCCACAGTGAATCTCTAGTAGCTGCTGCCTGGATCCC
  TCAGACAACGTCAACTCTCCCTTCTCCCTTTTTCTTATTCAATGTCATCTCAAGTGTTTATCCAATTCAC
  CTGCAGGATAAAATGTCTCCAGTGCTTCCCCACACACATCCTCTGTCCTATCTGGACCTCCAACCTCCAC
  CCTTAATTCTGTCTCCACTTGAATTTCAGTCCCCAGCTTTGAATCAAGTCCATTTTCAATCCCCTCTCCC
  AGTCCTACTACCCTCTTCTCTACCCTATAGTAGGGATTCTGGAGCATGTTATTCTCAATCACAAAGTAAG
  CCACACTGCCCCACAACTGAAATACAATACAGAGAAAGGCCCTTAGTTACAAAACAACTAGAAAGTAGAT
  TGACTTTACCCTTGATGGTCCAGAAAGATGCTTATAGCATCTTGGCCTCCAACCCTTCCCATGACTGGGC
  AGTCCCTATCTTTCCTGATAATTTCCCCATAAGCTGTGAGCTCCGGGATAAACTGGAGCAACACATTCAA
  AAGTGGTTCATTCAACACCAATGGTATTTTCCCCCTGAGATCCAAGAGTCTGAGGAAGTGATGGGGCTTC
  AGAATTCAGTAACAGGAAATTATCAAATCAGAGACCAACCTTGCACCTCACAAGCCCATATGTCTACTGG
  TGAACACAACAAGCAGGGCCAGAAGGTGAAATTTCAGCTAGAAAATGAATCTTGCAAAAATCTTGGACCT
  ATTCTAGGAAAAGGCCCAAAAGATCCAGTAAAGGGCTTGGAAAAAAATGTAGTAAAGGTTCAGGGGGCAA
  ACATTTTGGAGCCAGAAAGAAATTTAGTTAAGCACTTCAAGAGTGATTCCAGAAATGACTCATCAAATTA
  CATAGACGAAGATCTAGAAAACAACTGGAAAGCTAATTTTGGTACAAAGTCAGGGCAGATAAATCAAGGT
  CTGACCCCTCTGAGTATGCGACAATCCTGGCTTGCTATGAACGATAGCTTTTGTGACACTGACATCCATA
  TGGAAACCAGAAATTTAACAGCCATAAAGAGTTCAGAAAAGTTCATGTGCTCTCGAGAGAAGCTTGCCTT
  TCTCAACCCAGAAACTCGACAGACCCTGGAGGAACATGTTGTAAGGTTTTGGGTGAAGCACAGATGGGGT
  CTACCCCTTAAAATGCTCAAGCCCATAAACCTTTTCAAGTTAAAAAGTACTGAGTCCTTGCCTCTTACAC
  TCTGTACCCAACCTTCCTCAACCAATTCTGTACATAGGACCAATTCAGCAGTTGAGGTAGTCAGATTCCT
  AGGAAAACCATGCTTGAGACAGATAATAATTAAGGATTCTTCACCACCACCGGGGAATCTTCTCCTTGTC
  TCATCTCCTTCCTGTAAGAGGGCGGCAATTAGACGGCCTCCCTCTGGTGTTGACCATGAGCCTTCTGTAG
  CCCTACCAAACAAACCACAGAGTGCGTATCTTGCAGAAACTCTCGCTTATAATTTTATGGAAACGCCCAC
  TCAGAGTGGGGGTGTCTTAAAGGACGAGATGGAGACCGAAGAAGTCCTTTTACTGCCTAGAATGACTTGT
  GCCCCAAATCTAAGGGCACACAACCCTCAGGCAAAAGTTGTTAGTGAGTATCCATATAATGTGGAGACAG
  AATTAACAGGCCAGCCACAAATTTATACCACTTCTGTGCTCCTTCCAAAGCATTCCAGGAGCATGCCTCT
  TCCTGTAGACACTTTGACTTCAAATGCATTAGGTGACATTGTGATGGTAGACGGGGGCAATGGCCTGGTG
  CAGCAGAGACCCAGTACTCCAAAGCATCAAGTCTCACAGAAGGGCCAAATCAAGATGTTGGTCCCTACTT
  ATCAAGGAGAGGACACTAAGAGACAGAGTGAAAGAAAGTATGAAGACATGTCCAAGTTCAGCCCAGTCAC
  GGAGAGAAAAAACAACTTTGAAAACCAATACTATCAGACCCTTCCAAAAACCACACAGGTTCTTCCAGAA
  AGCCTCTTCCAAAGACTTTTAAGCCGATTTCTACCGCGGGTTCACCCCAAGAAAACAATCAAAGGGCAGG
  AATGTCATCCCCAAAAAGATAATCCTACAGCAGCCACTGCCCAGAACCAACGAAGGCAAGTGAGAAAGCA
  ACCATATATGGATGACAATGTTACTGAGGCCCAGAAACTTATGACAACTGTTGGACAGATGTTAGAAAAG
  AAAATGATGCAACATCAACACCATGCTTCAAAGGTCAACCAGCCTAGGGTAGTTCCTCCAGGCCCTACTT
  CTTGGTTTTCCCATGGCCACATGCCAGTCTCCTGCTTAAAGCGAAGAAGACCACCCAGTTACCCAGCCAA
  TTCCTTATGTCAGAGGCGTTATATACAGGGCAGGCACAACAGAGGCCAACTGCCTCAGAAAAGTGTACAA
  TTTAGCCAGAAACCACAGATCCTACGGAACCCCCGTCACATGTACCCCAAGTCAGCTTTGAACGTAGTGA
  ATTCTTCCCAGAATAGAACAACAGTGCCAAGAACCCCAAATCAACATCTTTGTTGTCCTAGGCACTGTGC
  CTTCCAGAGAAATGTCTGTAGAGAACTAGGACATCCCTCCGTAGCTTTTCCTAATAGGAAAACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR207328 representing NM_001008359
  Red=Cloning site Green=Tags(s)
  
  MENLPSLLESIYTTWLSLSSTIWAMDMILAFVCGLGLYLLLLPFLESHLSSSPSDINFTRKPQIQMTWQS
  QYMKKFRNHCRNDAKAWEECLKKLKEKEKDELFLEEMSPGHHLNSLGNMFNSASAKQDSTTLSPFWNLKE
  KSEEQMATQKLSYPKISGDHLQEKYDQLYWGLPSLHSESLVAAAWIPQTTSTLPSPFFLFNVISSVYPIH
  LQDKMSPVLPHTHPLSYLDLQPPPLILSPLEFQSPALNQVHFQSPLPVLLPSSLPYSRDSGACYSQSQSK
  PHCPTTEIQYRERPLVTKQLESRLTLPLMVQKDAYSILASNPSHDWAVPIFPDNFPISCELRDKLEQHIQ
  KWFIQHQWYFPPEIQESEEVMGLQNSVTGNYQIRDQPCTSQAHMSTGEHNKQGQKVKFQLENESCKNLGP
  ILGKGPKDPVKGLEKNVVKVQGANILEPERNLVKHFKSDSRNDSSNYIDEDLENNWKANFGTKSGQINQG
  LTPLSMRQSWLAMNDSFCDTDIHMETRNLTAIKSSEKFMCSREKLAFLNPETRQTLEEHVVRFWVKHRWG
  LPLKMLKPINLFKLKSTESLPLTLCTQPSSTNSVHRTNSAVEVVRFLGKPCLRQIIIKDSSPPPGNLLLV
  SSPSCKRAAIRRPPSGVDHEPSVALPNKPQSAYLAETLAYNFMETPTQSGGVLKDEMETEEVLLLPRMTC
  APNLRAHNPQAKVVSEYPYNVETELTGQPQIYTTSVLLPKHSRSMPLPVDTLTSNALGDIVMVDGGNGLV
  QQRPSTPKHQVSQKGQIKMLVPTYQGEDTKRQSERKYEDMSKFSPVTERKNNFENQYYQTLPKTTQVLPE
  SLFQRLLSRFLPRVHPKKTIKGQECHPQKDNPTAATAQNQRRQVRKQPYMDDNVTEAQKLMTTVGQMLEK
  KMMQHQHHASKVNQPRVVPPGPTSWFSHGHMPVSCLKRRRPPSYPANSLCQRRYIQGRHNRGQLPQKSVQ
  FSQKPQILRNPRHMYPKSALNVVNSSQNRTTVPRTPNQHLCCPRHCAFQRNVCRELGHPSVAFPNRKT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001008359
• ORF Size: 3144 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001008359.2, NP_001008360.2
• RefSeq Size: 3298 bp
• RefSeq ORF: 3147 bp
• Locus ID: 353306
• UniProt ID: B6VQA5
• Cytogenetics: 17p14
• MW: 119.6 kDa
• Gene Summary: protein that may be involved in spermatogenesis [RGD, Feb 2006]