Gp1ba (NM_001109654) Rat Tagged ORF Clone

CAT#: RR206791

Gp1ba (Myc-DDK-tagged ORF) - Rat glycoprotein Ib (platelet), alpha polypeptide (Gp1ba), (10 ug)

Product Images

Circular map for RR206791

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gp1ba
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206791 representing NM_001109654
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCTCCTCATCTTGCTGTTCCTGCTGCCAAGTCCCTTACACTCCCAGCCCACTTGTGATGTCTCCA
  AAGTGACCAGCCTGCTGGAAGTAAACTGTGAGGACAAGAAGCTGAAAAAATTGCCTACAGACCTGCCAGC
  CGACACAGGCATCCTCCACCTGAGCAAGAACCAACTGGGCACCTTCTCCACATCATACCTGGTGCATTTC
  ACTCACCTTACTCACCTGTACCTGAGTAGATGTGAGCTGACCAGCCTGCAGGCCCATGAGAAACTGTTAA
  AGCTGGAAACCCTGGACTTATCCCACAATCACCTGCAAAGTCTGCCCTCCCTAGGACGGGCACTGCCTGC
  ACTCACTACTCTAGACGTCTCCTTCAACAAGCTGGGTTCACTGTCTCCTGGTGCCCTGGAAGGCCTGGGC
  CAACTCGAGGAACTCTACTTGCAGAACAATGATCTTAAGAGTCTGCCCCCTGGGCTCTTAATGCCCACAA
  CTAAGCTGTTGAAACTCAGTCTGGCCAACAACAAACTTCGTGAGCTGCCCCCTGGGCTCCTAGATGGACT
  AGAGGATCTTGACACCCTCTACCTTCAAGGGAACTGGCTCAGCACAATACCAAAGGGCTTCTTTGGGTCC
  CTCCTCTTGCCTTTTGTTTTTCTCCATGCCAACACCTGGTACTGTGATTGTGAAATCCTCTACTTCCGTA
  ACTGGCTCCAAGAAAACCCCAACCGTGTCTACTTATGGAAGGAGGGTGTAGATGTCAAGGCCATGACTGC
  TAACGTGGCCAGTGTACGATGTGCCAATTTGGGCAATGCGCCTGTCTACTCCTACCCAGGGAAGGGCTGC
  CCTACCAATGAGGGTGACATAGACTATGATGACTATGCTGATACCCCTACCACAAGGACTGAGGTGAAGT
  TCTCTACTAACACTAAAGCTCATACTACCTACTGGGGCCTATTCTACTCAGAGTCTTCCACTTCTCCAGA
  CAGCCAAATGACTTCTTTGCCTCCCATCCACGAACCCACTAAGAAACAACCTACATTTGCCCACACAGAG
  ATCCCAGGTTTCACCACACTCCCAGATACCATGGAATCCAACACAGCCTTCTACAGTCTAAACAATACTG
  TACCCACCACCACCCCAGAGCCCACCTCCACCCAGGCTACCCCAGAGCCCAGCAGCACCCCAATGCTTAC
  CACTCCTGTGCCTACCCCAGAGCCCAGCAGCATCCCAATGCTTACCGTTCCTGTGTCTACCCCAGAGCCC
  AGCAGCACCCCAATGCTTACCACTCCCGTGTCTACCCCAGAGCCCAGCAGCATCCCAATGCCTACCATTC
  CCGTGCCTACCCCAGAGCCCAGCAGCATCCCAATGCCTACCATTCCCGTGCCTACCCCAGAGCCCAGCAG
  CACCCCAATGCCTACCATTCCTGTGCCTACCCCAGAGCCCAGCAGCACCCCAATGTTTACCACACCTGTG
  CCTACCCCAGAGCCCAGCAGTACCCCAATGCTTACCACTCCCACCCTGACTACCCCAGAGTCCAGCAGCA
  CCCCAAGTATTCTAGTGTCTACTATCCCCCCAGTGCTCACTACCACCCTGGCCCCTCCAGAGTCTATCCC
  AAAGACCGTTTTAGAACAATTTTATACCCTAGAGCCCACTTTACCCCGTACCTTAGAATCCACAATAATA
  ATCCCAGAGCAGAATAGTCTAAATTCCCAAGCGGTGCCTCAAGCAAGTTCTGATACTCCTAAAAGCAATC
  CTTTTCTCAACTCTGACTTCTGTTGCCTCCTCCCCCTGGGCTTCTATGTTCTGGGTCTCCTCTGGCTCTT
  ATTTGCCTCTGTGGTCCTCATTGTGTTGCTGACCTGGATCTGGCATGTGACACCGCACAATCTAGACATG
  GACCAATCTGCTGCACTGGCCACAAGCACACACACCACAAGCCTGGAAATGCAGAGAGCAAGGCAAGTAA
  CTATGCCCTGGGCCTGGCTGCTCTCCCTTCAAGGATCACTCCCTACTTTCCGGTCTAGCCTTTTCCTGTG
  GGTAAGGCCTAATGGGCGTGTGGGGCCTCTGGTAGCAGGACGGAGACCCTCAGCTCTGAGTCAGGGTCAT
  GGTCAGGACCTATTGGGCAGAGTGGGTATTAGGTACTCAGGCCACAGTCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206791 representing NM_001109654
  Red=Cloning site Green=Tags(s)
  
  MALLILLFLLPSPLHSQPTCDVSKVTSLLEVNCEDKKLKKLPTDLPADTGILHLSKNQLGTFSTSYLVHF
  THLTHLYLSRCELTSLQAHEKLLKLETLDLSHNHLQSLPSLGRALPALTTLDVSFNKLGSLSPGALEGLG
  QLEELYLQNNDLKSLPPGLLMPTTKLLKLSLANNKLRELPPGLLDGLEDLDTLYLQGNWLSTIPKGFFGS
  LLLPFVFLHANTWYCDCEILYFRNWLQENPNRVYLWKEGVDVKAMTANVASVRCANLGNAPVYSYPGKGC
  PTNEGDIDYDDYADTPTTRTEVKFSTNTKAHTTYWGLFYSESSTSPDSQMTSLPPIHEPTKKQPTFAHTE
  IPGFTTLPDTMESNTAFYSLNNTVPTTTPEPTSTQATPEPSSTPMLTTPVPTPEPSSIPMLTVPVSTPEP
  SSTPMLTTPVSTPEPSSIPMPTIPVPTPEPSSIPMPTIPVPTPEPSSTPMPTIPVPTPEPSSTPMFTTPV
  PTPEPSSTPMLTTPTLTTPESSSTPSILVSTIPPVLTTTLAPPESIPKTVLEQFYTLEPTLPRTLESTII
  IPEQNSLNSQAVPQASSDTPKSNPFLNSDFCCLLPLGFYVLGLLWLLFASVVLIVLLTWIWHVTPHNLDM
  DQSAALATSTHTTSLEMQRARQVTMPWAWLLSLQGSLPTFRSSLFLWVRPNGRVGPLVAGRRPSALSQGH
  GQDLLGRVGIRYSGHSL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001109654
• ORF Size: 2151 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001109654.1, NP_001103124.1
• RefSeq Size: 2677 bp
• RefSeq ORF: 2154 bp
• Locus ID: 691992
• Cytogenetics: 10q24
• MW: 78.3 kDa