Fbxo17 (NM_001013064) Rat Tagged ORF Clone

CAT#: RR206465

Fbxo17 (Myc-DDK-tagged ORF) - Rat F-box protein 17 (Fbxo17), (10 ug)

Product Images

Circular map for RR206465

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fbxo17
• Synonyms: Fbg4; Fbx17; Fbxo26
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206465 representing NM_001013064
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGCCCAGCCCTCGCGACGGCGGATGACCGAAGCTCAGCACATGGCTTTGGCCGAGCTGCCCCCGG
  AGTTGCTGCTGCAGGTGCTGAGCCATGTGCCTCCTCGCGCGTTAGTGACCCGCTGCCGGCCGGTGTGCCG
  CGCCTGGCGCGACCTAGTAGATGGCCCAAGCGTATGGCTGCTGCAACTGGCTCGCGACCGCAGCGCGGAG
  GGCAGAGCCCTCTACGCCTTGGCCCAGAGCTGCCCGGCCGACCGCAACGGCCACGACGACTTCCCACTCT
  GTGCTCTGGCCCGCTTCTGCCTGCTAGCGCCGCTTGGCCGCAACCTCATCTACAACTCCTGCGGAGAACA
  GGGCTTCAGAGGTTGGGAAGTGGAACATGGCGGGAACGGCTGGGCTGTGGAAAAGAATCTGACGATGGTG
  CCAGGGGCTCCTTCTCAAACCTGCTTCGTGACTTCTTTTGAGTGGTGCTTCAAGAGGCAGCTTGTAGACC
  TGGTGAAGGAAGGAGTGTGGCAGGAGCTGCTGGACAGTGCCCAGATTGAGATCTACATAGCTGACTGGTG
  GGGCGCCCGTGAGAACTGCGGCTGCATCTACAGGCTTCGGGTCCGCCTACTGGACGAACATGAAAACGAA
  GTGGTCAAGTTCTCTGCCTCACCCAACCCAGTCCTTCAGTGGACTGAGAGGAGCTGCCGACAAGTCTCTC
  ACGTCTTCACTGACTTTGGCAAGGGCATCCGCTATGTGTCCTTTGAGCAGTATGGAAGAGACACTCGTTC
  CTGGGTGGGACACTATGGTGCCCTCGTGACCCACTCCACTGTGCGGGTCAGGATCCGTCTGTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206465 representing NM_001013064
  Red=Cloning site Green=Tags(s)
  
  MGAQPSRRRMTEAQHMALAELPPELLLQVLSHVPPRALVTRCRPVCRAWRDLVDGPSVWLLQLARDRSAE
  GRALYALAQSCPADRNGHDDFPLCALARFCLLAPLGRNLIYNSCGEQGFRGWEVEHGGNGWAVEKNLTMV
  PGAPSQTCFVTSFEWCFKRQLVDLVKEGVWQELLDSAQIEIYIADWWGARENCGCIYRLRVRLLDEHENE
  VVKFSASPNPVLQWTERSCRQVSHVFTDFGKGIRYVSFEQYGRDTRSWVGHYGALVTHSTVRVRIRLS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001013064
• ORF Size: 834 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001013064.2, NP_001013082.2
• RefSeq Size: 1639 bp
• RefSeq ORF: 837 bp
• Locus ID: 292757
• UniProt ID: Q6AY27
• Cytogenetics: 1q21
• MW: 31.7 kDa
• Gene Summary: Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Able to recognize and bind denatured glycoproteins, which are modified with complex-type oligosaccharides. Also recognizes sulfated glycans. Does not bind high-mannose glycoproteins (By similarity).[UniProtKB/Swiss-Prot Function]