Sbf2 (NM_001134970) Rat Tagged ORF Clone

CAT#: RR206376

RGD1559442 (Myc-DDK-tagged ORF) - Rat similar to SET binding factor 2 (LOC691036), (10 ug)

Product Images

Circular map for RR206376

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Sbf2
• Synonyms: Mtmr13; RGD1559442
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206376 representing NM_001134970
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCGGTTGGCCGACTACTTCATTGTGGTAGGCTACGACCACGAGAAGCCAGGGCCAGGAGAAGGTC
  TGGGGAAAATAATTCAGAGATTCCCACAGCAGGACTGGGATGACACACCCTTTCCACAGGGAATTGAATT
  GGGAACGAAGAAGGAAGAGATCGAGGGTGAAGAAGTATCTGCTTTAATTCAACCTGCAGAAGTATTTGCA
  CCCAAAAGCCTGGTGTTGGTATCCAGATTAGACTATCCAGAAATCTTTAGGGCTTGCCTGGGTCTGATCT
  ATACTGTGTACGTGGACAGCCTGAGTGTCTCCTTGGAAAGCCTCATCGCAAATCTTTGTGCTTGCCTTGT
  TCCAGCTGCTGGAGGGTCTCAGAAGCTGTTTTCCTTGGGTGCAGGAGACAGACAACTGATTCAGACTCCT
  TTGCATGACAGCCTCCCTGTTACAGGCACCAGTGTGGCTCTTTTATTTCAGCAATTAGGCATTCAAAACG
  TCCTCAACCTCTTTTGTGCAGTCCTTACAGAAAATAAGGTTCTCTTCCATTCAGCAAGTTTCCAGAGACT
  TAGTGATGCCTGTAGAGCCCTGGAATCTTTAATGTTTCCTCTTAAATACAGTTACCCTTATATTCCCATT
  CTTCCGGCTCAGCTCCTAGAAGTTTTGAGTTCTCCAACTCCTTTCATTATTGGCGTACACTCCATCTTTA
  AAACTGACGTCCATGAGCTCTTAGATGTAATCATAGCAGATTTGGATGGAGGCACTATTAAAATTCCTGA
  ATGCATTCACCTCTCTTCCCTCCCGGAACCACTTCTTCATCAGACTCAAGCAGCACTTTCTCTGATTTTG
  CACCCGGATTTGGAAGTAGCAGATCATGCCTTCCCTCCTCCACGAACAGCTTTATCCCACTCAAAAATGC
  TGGATAAAGAAGTTCGAGCAGTTTTTCTTAGACTATTTGCACAACTGTTCCAAGGGTACAGGTCATGCCT
  CCAGCTTATACGGATCCATGCCGAGCCTGTGATACATTTTCACAAAACAGCTTTCTTGGGGCAGCGTGGT
  TTGGTTGAGAATGATTTCCTCACTAAAGTACTCAATGGAATGGCATTTGCAGGTTTTGTTTCAGAAAGAG
  GACCTCCCTATAGAGCCTGTGATCTCTTTGATGAGTTGGTAGCTTTTGAAGTAGAGAGAATTAAAGTTGA
  AGAAAATAATCCACTGAAGATGATAAAGCACATCAGAGAACTTGCTGAGCAACTCTTCAAAAATGAGAAT
  CCAAACCCTCATATGGCATTCCAGAAAGTTCCACGTCCAACAGAAGGATCCCACTTGAGAGTTCATATTC
  TTCCTTTCCCAAAAATTAATGAAGCCCGAGTTCAAGAACTAATACAGGAAAATCTTGCTAAGAACCAGAA
  TGCAACTCCTGCTACACGAACAGAAAAGAAATGCATAGTGCCAGCAGGGCCACCTGTTGTTTCAATAATG
  GATAAAGTGATAACAGTTTTTAACAGTGCACAGAGACTGGAAGTTGTTAGAAACTGCATCTCATTCATAT
  TTGAAAATAAAACTTTGGAGACTGAGAAGACTCTTCCCGCTGCCTTGAGGGCCCTTAAAGGAAAAGCAGC
  AAGACAGTGTCTCACTGATGAACTGGGTTTGCATGTTCAGCAGAACCGGGCAATATTGGACCATCAACAG
  TTTGACTACATAATAAGGATGATGAATTGTACTCTGCAGGATTGTTCAAGTTTGGAAGAGTATAACATCG
  CTGCAGCACTGCTCCCCCTGACCAGTGCTTTCTATAGGAGACTCGCCCCAGGAGTCAGCCAGTTTGCCTA
  CACGTGTGTGCAGGACCACCCCATCTGGACTAATCAGCAGTTTTGGGAGACAACGTTTTACAGTGCAGTG
  CAGGAGCAGGTTCGCTCCCTGTACCTCTCAGCGAAGGAAGACAATCATACCCCACATCAGAAGCAGAAGG
  AGCTTCCCGATGGCCAATATCAGGAGAGGACGGCCATGGACTTGGCAGCCGAGCAGCTTCGCCTTTGGCC
  TACTCTGAGCAAGGCAACTCAGCAGGAGCTGGTGCAGCACGAGGAAAGCACTGTCTTCAGTCAGGCCATT
  CACTTTGCAAACCTCATGGTTAACCTGCTAGTTCCACTAGACACAAGTAAAAACAAGCTCCTTCGAGCCT
  CAGCTCCAGGAGACTGGGAGAGTGGGAGCAACAGCATTGTCACAAACAGTATTGCAGGAAGTGTGGCTGA
  GAGCTTTGATACGGAGAGTGGATTTGAAGATTCGGAAAACAATGATGTGGCCAACTCTGTTGTAAGGTTC
  ATTGCCCGGTTTATTGACAAGGTTTGTACAGAGAGTGGAGTGACTCAGGACCACATCAGGAGTCTCCACT
  GCATGATACCAGGAATTGTAGCTATGCACATTGAGACCCTAGAGGCAGTACATCGAGAGAGTAGAAGACT
  TCCACCTATCCAGAAGCCCAAGATTCTTCGGCCAGCTCTACTGCCAGGAGAAGAAATTGTATGCGAAGGT
  CTTCGAGTCCTACTAGATCCTGATGGCCGGGAAGAAGCCACTGGAGGTCTCCTTGGAGGTCCTCAGCTCT
  TGCCTGCAGAAGGCGCCTTGTTCCTCACCACATACAGAATTCTCTTCAGAGGGACGCCACATGACCAGCT
  AGTTGGTGAACAAACAGTCGTGCGGAGCTTTCCCATTGCTTCCATCACCAAGGAGAAGAAGATAACCATG
  CAGAACCAGCTACAGCAGAACATGCAAGAAGGGTTGCAGATTACATCAGCATCTTTCCAGTTGATTAAAG
  TAGCATTTGATGAAGAAGTCAGCCCAGAAGTGGTAGACATCTTTAAGAAACAGCTAATGAAGTTCCGTTA
  CCCTCAGTCCATTTTCAGCACCTTCGCTTTTGCTGCTGGACAAACTACCCCACAAATCATTTTACCCAAG
  CAGAAGGAAAAGAACACCTCCTTCCGAACTTTCTCAAAAACAATTGTGAAAGGTGCCAAGAAGGCAGGAA
  AAATGACAATTGGGAGGCAGTATTTATTAAAGAAGAGAACAGGAACAATTGTGGAAGAGAGGGCAAATCG
  TCCAGGGTGGAATGAAGAAGATGATATTTCTGTTTCAGATGATTGTGAACTCCCCACAAGTACAACTCTG
  AAGGCCTCAGAGAAATCTACAATGGAACAGTTGGTGGAAAAGGCATGTTTCAGAGATTATCAGCGGTTAG
  GTTTGGGAACCATAAGTGGCAGCTCCTCCCGTTCAAAACCAGAGTATTTCCGAGTCACTGCCTCCAACAG
  GCTATATTCGCTGTGCCGGAGCTATCCTGGCCTTTTAGTCATACCTCAAGCTGTACAAGACAGTAGTTTA
  CCAAGAGTAGCCCGCTGCTATCGGCACAATCGCCTGCCCGTCGTATGTTGGAAGAATTCAAGAAGTGGTA
  CTCTGCTCCTCCGATCTGGAGGATTCCATGGGAAGGGAGTTGTTGGTCTGTTCAAATCACAGAACTCCCC
  TCAAGCCGTTTCTACCTCCTCTTTAGAATCTTCCAGTAGCATAGAGCAAGAAAAGTACTTGCAAGCCCTA
  TTGACTGCAGTTATTGTCCATCAGAAACTCAGAGGCAACAGTACTCTTACTGTCAGGCCAACACTTGCTC
  TGTCCCCAGTGCATGGGTACAGAGACAAATGTTTCACTCAGTCAAATTCCAAATCTTCAGCTAAGGAACC
  AGTTCATAATCAAGGCGTCTGGGCAAGTCTTCGTTCCAGCACCCGTTTGATCAGCTCCCCAACATCCCTC
  ATTGATGTTGGTGCCAGGCTGGCAGGCAAGGACCATTCAGCCTCCTTCAGTAACAGCACCTACCTACAAA
  ACCAGCTCTCGAAACGACAAGCCGCCCTTTATATATTTGGTGAAAAGTCGCAGCTTAGGAGCTCAAAGGT
  AGAATTTGCTTTCAACTGTGAATTTGTTCCTGTTGAGTTCCATGAAATCCGACAAGTGAAAGCCAGTTTT
  AAGAAGCTGATGAGGGCTTGTATCCCAAGCACCATCCCTACAGACTCGGAAGTGACGTTCCTGAAGGCCC
  TGGAAGATTCTGAGTGGTTCCCACAGCTTCACAGGATAATGCAGCTGGCTGTGGTTGTGTCAGAAGTACT
  TGAGAACGGGTCTTCAGTTTGGGTGTGTTTAGAAGAAGGCTGGGATATCACTACACAAGTGACATCCCTA
  GTCCAGCTACTCAGTGATCCCTTCTACAGGACAATAGAAGGCTTCCGGATGTTGGTTGAAAAAGAATGGC
  TCTCTTTTGGTCATAAGTTCAGCCAGCGCAGCAGCCTGGCCCTTAACTCTCAGGGCAGTGGTTTTGCTCC
  CATCTTCTTGCAGTTCTTAGACTGCGTGCACCAGGTTCACAACCAGTATCCAACTGAGTTTGAATTCAAT
  CTCTATTACCTAAAGTTCTTGGCTTTCCATTATGTGTCTAATCGCTTTAAAACATTTCTCCTGGATTCAG
  ACTATGAAAGACTAGAGCATGGAACTCTGTTTGATGATAAAGGCGACAAGCACGCCAAGAAAGGCGTCTG
  TATCTGGGAGTGCATTGACAAGATGCACAAGCGGAGCCCCGTTTTCTTCAATTACTTGTACTCACCAGTG
  GAAATAGAGGCTCTGAAGCCTAATGTAAATGTCTCCAGCCTAAAGAAGTGGGATTACTACACAGAAGAGA
  CCCTGTCTGCAGGTCCTTCATACGACTGGATGATGCTGACCCCCAAGCATTTTCCCTACGAGGAGTCAGA
  TGTAGCTGGAGGAGCTGGGCCCCAGAGTCAGAGGAAAACAGTGTGGCCATGCTATGATGATGTGACCTGC
  AGCCAGCCAGATGCCCTCACAAGACTTTTCAGTGAAATTGAAAAATTGGAGCATAAATTGAATCAAACTC
  CTGAGAAATGGCACCAGCTATGGGAAAAAATTACCATGGACCTCAAGGAAGAGCCAAGAACCGATCATTC
  CCTAAGACACTCGTCAAGGTCCCCAGGAATTGCATCTACCAATGTGCCTTCCTATCAGAAGAGGCCTGCA
  CTGCACGCGCTGCACCGCAGCCCGACAGAGGATCAAAGTACCACCACTGCCCCGTCCAATGGCGTAGAGC
  ACAGGGCGGCCACCCTTTACAGCCAGTACACATCCAAGAACGACGAGAACAGATCCTTTGAAGGAACATT
  ATACAAAAGAGGAGCCTTGCTGAAGGGGTGGAAACCCCGTTGGTTTGTTTTAGATGTAACAAAACACCAG
  CTTCGATATTATGACTCAGGGGAAGACACCAGCTGTAAAGGCCACATTGACCTGGCTGAGGTAGAAATGG
  TCGTCCCTGCCGGCCCCAGCATGGGAGCCCCAAAGTACACAAGTGACAAGGCTTTCTTTGATCTCAAGAC
  CAGCAAGCGAGTGTACAACTTCTGTGCCCAGGACGGACAGAGCGCCCAGCAGTGGATGGACAGGATCCAG
  AGCTGCATCTCCGATGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206376 representing NM_001134970
  Red=Cloning site Green=Tags(s)
  
  MARLADYFIVVGYDHEKPGPGEGLGKIIQRFPQQDWDDTPFPQGIELGTKKEEIEGEEVSALIQPAEVFA
  PKSLVLVSRLDYPEIFRACLGLIYTVYVDSLSVSLESLIANLCACLVPAAGGSQKLFSLGAGDRQLIQTP
  LHDSLPVTGTSVALLFQQLGIQNVLNLFCAVLTENKVLFHSASFQRLSDACRALESLMFPLKYSYPYIPI
  LPAQLLEVLSSPTPFIIGVHSIFKTDVHELLDVIIADLDGGTIKIPECIHLSSLPEPLLHQTQAALSLIL
  HPDLEVADHAFPPPRTALSHSKMLDKEVRAVFLRLFAQLFQGYRSCLQLIRIHAEPVIHFHKTAFLGQRG
  LVENDFLTKVLNGMAFAGFVSERGPPYRACDLFDELVAFEVERIKVEENNPLKMIKHIRELAEQLFKNEN
  PNPHMAFQKVPRPTEGSHLRVHILPFPKINEARVQELIQENLAKNQNATPATRTEKKCIVPAGPPVVSIM
  DKVITVFNSAQRLEVVRNCISFIFENKTLETEKTLPAALRALKGKAARQCLTDELGLHVQQNRAILDHQQ
  FDYIIRMMNCTLQDCSSLEEYNIAAALLPLTSAFYRRLAPGVSQFAYTCVQDHPIWTNQQFWETTFYSAV
  QEQVRSLYLSAKEDNHTPHQKQKELPDGQYQERTAMDLAAEQLRLWPTLSKATQQELVQHEESTVFSQAI
  HFANLMVNLLVPLDTSKNKLLRASAPGDWESGSNSIVTNSIAGSVAESFDTESGFEDSENNDVANSVVRF
  IARFIDKVCTESGVTQDHIRSLHCMIPGIVAMHIETLEAVHRESRRLPPIQKPKILRPALLPGEEIVCEG
  LRVLLDPDGREEATGGLLGGPQLLPAEGALFLTTYRILFRGTPHDQLVGEQTVVRSFPIASITKEKKITM
  QNQLQQNMQEGLQITSASFQLIKVAFDEEVSPEVVDIFKKQLMKFRYPQSIFSTFAFAAGQTTPQIILPK
  QKEKNTSFRTFSKTIVKGAKKAGKMTIGRQYLLKKRTGTIVEERANRPGWNEEDDISVSDDCELPTSTTL
  KASEKSTMEQLVEKACFRDYQRLGLGTISGSSSRSKPEYFRVTASNRLYSLCRSYPGLLVIPQAVQDSSL
  PRVARCYRHNRLPVVCWKNSRSGTLLLRSGGFHGKGVVGLFKSQNSPQAVSTSSLESSSSIEQEKYLQAL
  LTAVIVHQKLRGNSTLTVRPTLALSPVHGYRDKCFTQSNSKSSAKEPVHNQGVWASLRSSTRLISSPTSL
  IDVGARLAGKDHSASFSNSTYLQNQLSKRQAALYIFGEKSQLRSSKVEFAFNCEFVPVEFHEIRQVKASF
  KKLMRACIPSTIPTDSEVTFLKALEDSEWFPQLHRIMQLAVVVSEVLENGSSVWVCLEEGWDITTQVTSL
  VQLLSDPFYRTIEGFRMLVEKEWLSFGHKFSQRSSLALNSQGSGFAPIFLQFLDCVHQVHNQYPTEFEFN
  LYYLKFLAFHYVSNRFKTFLLDSDYERLEHGTLFDDKGDKHAKKGVCIWECIDKMHKRSPVFFNYLYSPV
  EIEALKPNVNVSSLKKWDYYTEETLSAGPSYDWMMLTPKHFPYEESDVAGGAGPQSQRKTVWPCYDDVTC
  SQPDALTRLFSEIEKLEHKLNQTPEKWHQLWEKITMDLKEEPRTDHSLRHSSRSPGIASTNVPSYQKRPA
  LHALHRSPTEDQSTTTAPSNGVEHRAATLYSQYTSKNDENRSFEGTLYKRGALLKGWKPRWFVLDVTKHQ
  LRYYDSGEDTSCKGHIDLAEVEMVVPAGPSMGAPKYTSDKAFFDLKTSKRVYNFCAQDGQSAQQWMDRIQ
  SCISDA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001134970
• ORF Size: 5478 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001134970.1, NP_001128442.1
• RefSeq Size: 6383 bp
• RefSeq ORF: 5481 bp
• Locus ID: 691042
• Cytogenetics: 1q33
• MW: 205.6 kDa