Pygl (NM_022268) Rat Tagged ORF Clone

CAT#: RR206321

Pygl (Myc-DDK-tagged ORF) - Rat phosphorylase, glycogen, liver (Pygl), (10 ug)

Product Images

Circular map for RR206321

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pygl
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206321 representing NM_022268
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGAAGCCCTTGACCGACCAGGAAAAGCGACGGCAGATCAGCATCCGCGGCATCGTGGGCGTGGAGA
  ACGTAGCGGAGCTGAAAAAGGGCTTCAATCGTCACCTGCACTTCACTCTGGTCAAGGACCGCAATGTGGC
  CACCCCCCGCGACTACTACTTCGCCCTTGCGCACACAGTGCGCGACCACCTGGTGGGGCGCTGGATCCGC
  ACACAGCAGCACTACTATGACAAGTGCCCCAAGAGGGTGTATTACCTCTCTCTGGAATTTTACATGGGCC
  GAACATTACAGAACACCATGATCAACCTTGGCTTACAGAATGCCTGCGACGAAGCTATTTACCAGCTCGG
  GCTGGACATGGAGGAGTTGGAAGAAATTGAAGAAGATGCTGGGCTTGGCAATGGTGGTCTTGGGAGGCTT
  GCTGCCTGCTTCTTGGACTCCATGGCAACGCTGGGGCTTGCAGCCTATGGATACGGCATCCGTTATGAAT
  ATGGAATCTTCAATCAGAAGATCCGAGAAGGGTGGCAGGTAGAGGAGGCAGATGACTGGCTCAGGCATGG
  AAACCCTTGGGAGAAGGCTCGTCCTGAATTCATGCTGCCTGTGCATTTCTACGGAAGAGTAGAGCACACC
  CAGGCAGGAACAAAGTGGGTCGACACCCAGGTGGTGCTGGCTTTGCCGTACGACACCCCCGTACCTGGGT
  ATATGAACAACACGGTGAACACTATGCGCCTCTGGTCGGCCCGAGCACCCAATGACTTTAACCTTCAAGA
  CTTTAATGTCGGAGACTACATTCAGGCTGTGCTGGACCGGAACCTGGCTGAGAATATCTCCAGAGTGCTG
  TACCCCAACGATAACTTTTTTGAAGGGAAGGAGCTGAGGCTGAAGCAGGAGTACTTTGTGGTGGCTGCGA
  CCCTGCAGGATGTCATCCGACGTTTCAAGGCCTCCAAGTTCGGCTCCAAGGATGGTGTAGGAACCGTGTT
  TGATGCTTTTCCAGATCAGGTAGCCATCCAGCTGAATGACACACATCCCGCACTCGCCATCCCGGAGCTG
  ATGAGGATCTTTGTGGACATTGAAAAATTGCCCTGGTCCAAGGCCTGGGAGATCACCAAGAAGACCTTTG
  CCTACACCAACCACACGGTGCTCCCGGAGGCCCTGGAGCGCTGGCCAGTGGACCTGGTGGAGAAGCTGCT
  GCCTCGACACTTGCAGATCATTTATGAGATCAATCAGAAGCATTTAGATAGAATCGTGGCCCTGTTTCCT
  AAAGACATCGACCGCATGCGGCGGATGTCTCTCATCGAAGAGGAAGGAGGCAAAAGGATCAACATGGCCC
  ACCTCTGCATCGTGGGCTGCCACGCGGTGAACGGGGTAGCGAAGATCCACTCGGACATCGTGAAGACCCA
  AGTATTCAAGGACTTCAGTGAGCTAGAACCAGACAAGTTCCAGAATAAAACCAACGGGATCACCCCGAGG
  CGCTGGCTCTTACTCTGCAACCCAGGGCTGGCTGACTTGATAGCAGAGAAAATTGGAGAAGACTATGTGA
  AAGACCTGAGCCAGCTGACGAAGCTCCACAGCTTCGTGGGCGACGACATCTTCCTCCGGGAAATAGCCAA
  AGTGAAGCAGGAAAATAAACTGAAATTCTCCCAGTTCCTGGAAAAGGAGTACAAGGTGAAGATCAACCCA
  TCTTCCATGTTTGACGTGCACGTGAAGCGGATCCACGAGTACAAACGACAGCTTCTGAACTGCCTGCATG
  TGATCACCATGTACAATCGCATCAAGAAAGACCCTAAGAAGTTCTTCGTGCCAAGGACAGTCATAATTGG
  TGGGAAAGCTGCCCCAGGATATCACATGGCCAAAATGATCATAAAGCTGGTCACCTCCGTGGCAGAAGTG
  GTGAACAACGACCCTATGGTTGGCAGCAAGTTGAAAGTCATCTTCTTGGAGAACTACAGAGTGTCTCTTG
  CTGAAAAAGTCATTCCAGCCACGGACCTGTCAGAACAGATCTCCACTGCTGGCACGGAAGCCTCGGGGAC
  GGGCAACATGAAGTTCATGCTGAACGGGGCCCTGACCATCGGGACTATGGATGGGGCCAATGTGGAGATG
  GCGGAGGAGGCCGGGGAGGAAAACCTGTTCATCTTTGGCATGAGGGTAGATGATGTGGCCGCTCTGGACA
  AGAAAGGGTATGAGGCCAAAGAATATTATGAGGCCCTTCCAGAACTGAAGCTGGTCATTGACCAAATTGA
  CAATGGCTTCTTTTCTCCCAATCAGCCAGACCTCTTCAAAGACATCATCAACATGTTATTTTATCATGAC
  AGATTTAAAGTCTTTGCAGACTACGAAGCCTATGTCAAGTGTCAAGAAAAAGTCAGTCAGCTGTATATGA
  ATCAAAAAGCCTGGAACACAATGGTTCTCAGAAACATAGCTGCCTCGGGGAAGTTCTCCAGTGACCGAAC
  AATCAGGGAGTATGCCAAGGACATCTGGAACATGGAGCCTTCCGATCTGAAGATCTCCCTATCTAAGGAG
  TCCAGCAATGGGGTCAACGCCAATGGGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206321 representing NM_022268
  Red=Cloning site Green=Tags(s)
  
  MAKPLTDQEKRRQISIRGIVGVENVAELKKGFNRHLHFTLVKDRNVATPRDYYFALAHTVRDHLVGRWIR
  TQQHYYDKCPKRVYYLSLEFYMGRTLQNTMINLGLQNACDEAIYQLGLDMEELEEIEEDAGLGNGGLGRL
  AACFLDSMATLGLAAYGYGIRYEYGIFNQKIREGWQVEEADDWLRHGNPWEKARPEFMLPVHFYGRVEHT
  QAGTKWVDTQVVLALPYDTPVPGYMNNTVNTMRLWSARAPNDFNLQDFNVGDYIQAVLDRNLAENISRVL
  YPNDNFFEGKELRLKQEYFVVAATLQDVIRRFKASKFGSKDGVGTVFDAFPDQVAIQLNDTHPALAIPEL
  MRIFVDIEKLPWSKAWEITKKTFAYTNHTVLPEALERWPVDLVEKLLPRHLQIIYEINQKHLDRIVALFP
  KDIDRMRRMSLIEEEGGKRINMAHLCIVGCHAVNGVAKIHSDIVKTQVFKDFSELEPDKFQNKTNGITPR
  RWLLLCNPGLADLIAEKIGEDYVKDLSQLTKLHSFVGDDIFLREIAKVKQENKLKFSQFLEKEYKVKINP
  SSMFDVHVKRIHEYKRQLLNCLHVITMYNRIKKDPKKFFVPRTVIIGGKAAPGYHMAKMIIKLVTSVAEV
  VNNDPMVGSKLKVIFLENYRVSLAEKVIPATDLSEQISTAGTEASGTGNMKFMLNGALTIGTMDGANVEM
  AEEAGEENLFIFGMRVDDVAALDKKGYEAKEYYEALPELKLVIDQIDNGFFSPNQPDLFKDIINMLFYHD
  RFKVFADYEAYVKCQEKVSQLYMNQKAWNTMVLRNIAASGKFSSDRTIREYAKDIWNMEPSDLKISLSKE
  SSNGVNANGK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022268
• ORF Size: 2550 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022268.1, NP_071604.1
• RefSeq Size: 2715 bp
• RefSeq ORF: 2553 bp
• Locus ID: 64035
• UniProt ID: P09811
• Cytogenetics: 6q24
• MW: 97.5 kDa
• Gene Summary: catalyzes the rate-limiting step of glycogen catabolism [RGD, Feb 2006]