Laptm5 (NM_053538) Rat Tagged ORF Clone
CAT#: RR206170
- TrueORF®
Laptm5 (Myc-DDK-tagged ORF) - Rat lysosomal multispanning membrane protein 5 (Laptm5), (10 ug)
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_053538" in other vectors (3)
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Laptm5 |
Synonyms | gcd-10 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR206170 representing NM_053538
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTCCCGTGCAGTGCCAGTCAGGCAGACATGCTGTTGTTTCAATGTCCGAGTTGCCACCGTAGCCC TGGCCATCTACCATATAGTCATGAGCGTCTTGCTGTTCATTGAGCATGTGGTGGAGGTGGCCCGCGGTAA AGTCTCCTGCAGGTTCTCCAAGATGCCATACCTCAGGCTGGCTGACCTGCTCTCCAGCTTCCTGCTCATC GGAGTCCTCTTCATTATCAGCCTCAGCCTGCTGTTCGGAGTGGTCAAGAACCGGGAGAAGTACCTGATAC CCTTCCTGTCCCTTCAAATCATGGACTTCCTGCTGTGCCTGCTCACACTGTTGGGCTCCTACATCGAATT ACCAGCATACCTGAAGTTTGCCCGGCCCCGGCCCGGTCCTTCTAAGGTACCCTTGATGACACTGCAGCTG CTGGACTTCTGTTTGAGCATCCTGACCCTGTGCAGCTCCTATATGGAAGTGCCCACCTACCTTAACTTCA AGTCCATGAACCACATGAATTACCTGCCGAGTCAGGAGGGCATGCCACGCAGCCAGTTCATCAACATGAT GCTCATCTTTTCAGTGGCCTTTATCACCGTGCTCATCCTGAAGGTCTACATGTTCAAGTGTGTTTGGACA TGCTACAGATTCATGAAGCACATGAATTCGGCCGTGGAGGACAGCAGTTCCAAGCTGTTCCTAAAGGTGG CTCTGCCGTCCTATGAGGAAGCCTTGTCTCTGCCCACTAAGACTCCAGAGGGGGACCTTGCACCACCCCC ATACTCGGAAGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR206170 representing NM_053538
Red=Cloning site Green=Tags(s) MASRAVPVRQTCCCFNVRVATVALAIYHIVMSVLLFIEHVVEVARGKVSCRFSKMPYLRLADLLSSFLLI GVLFIISLSLLFGVVKNREKYLIPFLSLQIMDFLLCLLTLLGSYIELPAYLKFARPRPGPSKVPLMTLQL LDFCLSILTLCSSYMEVPTYLNFKSMNHMNYLPSQEGMPRSQFINMMLIFSVAFITVLILKVYMFKCVWT CYRFMKHMNSAVEDSSSKLFLKVALPSYEEALSLPTKTPEGDLAPPPYSEV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_053538 |
ORF Size | 783 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_053538.2, NP_445990.2 |
RefSeq Size | 2170 bp |
RefSeq ORF | 786 bp |
Locus ID | 89783 |
Cytogenetics | 5q36 |
MW | 29.6 kDa |
Gene Summary | a lysosomal protein involved in microglial activation [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN206170 | Laptm5 (untagged ORF) - Rat lysosomal multispanning membrane protein 5 (Laptm5), (10 ug) |
USD 330.00 |
|
RR206170L3 | Lenti ORF clone of Laptm5 (Myc-DDK-tagged ORF) - Rat lysosomal multispanning membrane protein 5 (Laptm5), (10 ug) |
USD 630.00 |
|
RR206170L4 | Lenti ORF clone of Laptm5 (mGFP-tagged ORF) - Rat lysosomal multispanning membrane protein 5 (Laptm5), (10 ug) |
USD 630.00 |
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