Iars (NM_001100572) Rat Tagged ORF Clone

CAT#: RR206137

Iars (Myc-DDK-tagged ORF) - Rat isoleucyl-tRNA synthetase (Iars), (10 ug)

Product Images

Circular map for RR206137

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Iars
• Synonyms: Iarsl
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206137 representing NM_001100572
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTCAACAAGTTCCGGAAAACATCAGCTTTCCTGCTGAAGAAGAAAAAATCTTACAGTTCTGGTCCA
  AATACAACTGTTTCCAGGAATGCCTAAAGCAGTCAAAGCTCAGGCCAAAGTTTACCTTCTATGATGGGCC
  TCCCTTCGCTACTGGACTGCCTCACTACGGACACATACTTGCGGGGACAATTAAAGATATAGTTACAAGA
  TACGCTCACCAGAGCGGGTTTCACGTCGACAGAAGATTCGGGTGGGATTGTCATGGCTTACCTGTGGAGT
  ACGAGATTGACAAGACCCTGGGGATCAAAGGGCCAGAGGACGTGGCCAAGATGGGGATCGCAGAGTACAA
  CAAGCAGTGCCGAGCTATTGTGATGAGGTACTCCGCCGAGTGGAAGTCTACTGTTACCAGGCTTGGCCGA
  TGGATCGACTTTGACAATGACTACAAAACCCTGTACCCACAATTCATGGAATCAGTCTGGTGGGTCTTCA
  AGCAGCTGTATGACAAAGGCCTCGTGTACAGAGGCGTGAAAGTCATGCCCTTCTCCACCGCGTGTGGAAC
  TCCACTCTCCAACTTCGAGTCGAACCAGAATTACAAGGACGTTCAGGACCCTTCAGTATTTGTAACGTTC
  CCTTTGGAGGAAGATGAAAACACATCCTTAGTTGCTTGGACAACCACTCCGTGGACCCTACCCAGTAACC
  TTGCGCTATGTGTCAATCCAGAAATACAGTATGTGAAGATCAAAGACGTTTCCAGGGGGAAGTTGTTCAT
  TTTGATGGAAGCCAGATTGTCGGCCCTCTATAAACAAGAGAGCGACTATGAGATCCTTGAAAGGTTTCCT
  GGTGCCTCTCTCAAAGGCAAGAAATACAAGCCGTTGTTTGACTACTTTATTAAGTGTAAGGAGAACGGTG
  CCTTCACCGTGCTTGTTGACCACTATGTGAAGGATGAAGAAGGCACAGGTGTCGTTCACCAGGCTCCTTA
  CTTTGGCGCTGACGATCATCGAGTCTGTATGGACTTCAACATCATTCAGAAAGACTCAGCCCCTGTTTGT
  CCTGTGGATGCTTCAGGCTGCTTCACAGAGGAAGTGACACATTTTGTCGGGCAGTATGTGAAGGATGCTG
  ACAAAAATATCATCAGGATGTTGAAGGAGCAGGGCCGACTGCTCGTGGCAAGCACCTTCACTCACAGCTA
  CCCTTTCTGCTGGAGGTCGGACACTCCCCTCATTTACAAGTCCGTGCCCAGCTGGTTCGTGCGGGTGGAG
  CACATGGTGGACCAGCTACTGAAGAACAATGATCTGTGTTACTGGGTCCCAGAGTTTGTACGAGAAAAAC
  GGTTTGGAAATTGGCTGAAGGAGGCACGTGACTGGGCAATTTCCCGAAACAGATACTGGGGCACCCCCAT
  CCCACTGTGGGTCAGTGAAGACTTTGAGGAGGTAGTGTGCATCGGGTCAGTGGCAGAACTTGAAGAATTG
  TCAGGAACAAAAATCTCAGATCTCCACAGAGAGAGCATCGACCACCTGACCATACCATCCCGCTGCGGGA
  AAGGACCGTTGCGGCGTGTCTCTGAGGTGTTTGACTGTTGGTTCGAGAGTGGAAGTATGCCTTACGCCCA
  GGTTCATTACCCCTTTGAGAGCAAGCGAGAGTTCGAGGATGCCTTTCCCGCAGACTTCATTGCTGAAGGC
  ATCGATCAAACCAGAGGATGGTTTTACACCCTGCTAGTGCTAGCCACAGCCCTCTTTGGACAACCACCTT
  TCAAGAACGTGATTGTGAACGGCCTCATCTTGGCAAGTGATGGCCAAAAAATGAGCAAACGGAAAAAGAA
  CTACCCAGATCCGGTTTCCATCATCAATAAGTACGGTGCCGACGCCCTCAGGTTATATCTGATCAATTCC
  CCTGTGGTGAGAGCAGAAAACCTGCGCTTCAAGGAGGAAGGCGTACGGGATGTCCTCAAAGACGTGCTGC
  TCCCATGGTACAACGCATACCGCTTCTTCATCCAGAACGTCTTTAGGCTGCACAAGGAAGAAGAAGTAGA
  GTTTCTGTACAACGAGCACACGGTCCGAGAAAGCCCCAACATCACCGACCGGTGGATTCTGTCCTTCATG
  CAGTCGCTCATTGCGTTCTTTGAGACGGAAATGGCAGCTTACAGGCTGTACACTGTGGTGCCTCGCCTGG
  TCAAGTTCGTAGATATTCTGACCAACTGGTACGTCCGAATGAACAGGAGAAGATTAAAGGGGGAGAATGG
  CATGGAAGACTGTGTTATGGCTCTGGAGACTCTGTTCAGTGTCCTGCTTTCTCTGTGCAGACTGATGGCC
  CCTTACACACCCTTTCTTACTGAACTGATGTACCAGAACCTGAAGCTGCTGATTGACCCTGCATCTATCC
  GGGACAAGGACACCCTCAGCATCCACTACCTCATGCTGCCTCATGTTCGAGAGGAGCTGATTGACAAGAA
  AACTGAGAACGCCGTGTCTAGAATGCAGTCCGTGATTGAGCTTGGGAGAGTGATTCGAGACCGGAAAACT
  ATTCCAATAAAGTATCCCTTGAAGGAAATTGTCGTCATCCACCAAGACCCAGAAGCTCTTGAGGATATCA
  AGTCCTTGGAGAAGTATATCATTGAGGAACTAAATGTTCGAAAAGTTACACTGTCTACTGATAAAAACAA
  GTATGGCATTCGACTGAGGGCTGAGCCAGATCACATGGTCCTTGGGAAACGTCTGAAGGGAGCCTTTAAA
  GCAGTGATGGTGGCCGTAAAGCAGCTGAGCAATGAGGAGCTGGAGCAGTTTCAGAAGAGTGGGAGCATCG
  TCGTGGAAGGCCATGAATTACACGAAGAAGATATTCGTCTCATGTACACCTTTGACCAGGCCACAGGAGG
  CACTGCACAGTTTGAAGCACACTCTGATGCTCAGGCTTTAGTTCTCTTAGATGTCACACCTGACCAGTCA
  ATGGTGGACGAAGGCATGGCTCGGGAAGTCATCAATCGCATCCAGAAGCTTCGCAAGAAGTGCAACCTGG
  TTCCGACGGATGAAATAACAGTTTACTATAATGCCAAATCTGAAGGAAGATACTTGAGTAATGTCATCGA
  AAGCCACACTGACTTCATATATGCCACTATCAAAGCTCCCTTGAAGCCCTATCCAGTTCCTACATCAGAT
  AATATCCTTATTCAAGAGCAAACACAGTTAAAGGGGTACGAGCTGGAAATCACGATCACCAGAGGATCCT
  GTGTGCCTGGGCCCGCTTGTGCTTATGTGAACCTTAACATTTGTGCAAATGGCACTGAGCAAGGTGGAGT
  GTTACTCCTGGAGAATCCGAAGGGTGATAATCAGTTGAACCTTGAGAAGCTGAAGAGCGTCATTACCAGC
  ATATTCGGTGTTAAAAGCACAGGGCTGTCAGTCTTCAACGGTGGAACAGAATTACAGAACCAGACTGACT
  TGCTGAGCCTCAGTGGAAGGACGCTGTGTGTGACTGCAGGAGCATCCCTGGCTCCAGCCAGCAGCCCCAG
  CACCCTTCTCTGTCAGTACATCAACCTGCAGCTCGTGAACGCAGAGCCACAAGAGTGTTTAACAGGAACT
  GTGGGCACCCTCCTGCTTGAAAACCCACTTGGACAGAACGGGCTCACCCACCAAGGGCTTGTGTACGAAG
  CAGCCAAGGTGTTTGGACTGCGCAGCCGGAGGCTAAAGCTGTTCCTGAATGAGACTCAGACACAAGAAAT
  TACAGAAGATATCCCAATGAAGACTTTGAATATGAAGACTGTATATGTTTCTGTCTTGCCAACGACTGCA
  GATGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206137 representing NM_001100572
  Red=Cloning site Green=Tags(s)
  
  MVQQVPENISFPAEEEKILQFWSKYNCFQECLKQSKLRPKFTFYDGPPFATGLPHYGHILAGTIKDIVTR
  YAHQSGFHVDRRFGWDCHGLPVEYEIDKTLGIKGPEDVAKMGIAEYNKQCRAIVMRYSAEWKSTVTRLGR
  WIDFDNDYKTLYPQFMESVWWVFKQLYDKGLVYRGVKVMPFSTACGTPLSNFESNQNYKDVQDPSVFVTF
  PLEEDENTSLVAWTTTPWTLPSNLALCVNPEIQYVKIKDVSRGKLFILMEARLSALYKQESDYEILERFP
  GASLKGKKYKPLFDYFIKCKENGAFTVLVDHYVKDEEGTGVVHQAPYFGADDHRVCMDFNIIQKDSAPVC
  PVDASGCFTEEVTHFVGQYVKDADKNIIRMLKEQGRLLVASTFTHSYPFCWRSDTPLIYKSVPSWFVRVE
  HMVDQLLKNNDLCYWVPEFVREKRFGNWLKEARDWAISRNRYWGTPIPLWVSEDFEEVVCIGSVAELEEL
  SGTKISDLHRESIDHLTIPSRCGKGPLRRVSEVFDCWFESGSMPYAQVHYPFESKREFEDAFPADFIAEG
  IDQTRGWFYTLLVLATALFGQPPFKNVIVNGLILASDGQKMSKRKKNYPDPVSIINKYGADALRLYLINS
  PVVRAENLRFKEEGVRDVLKDVLLPWYNAYRFFIQNVFRLHKEEEVEFLYNEHTVRESPNITDRWILSFM
  QSLIAFFETEMAAYRLYTVVPRLVKFVDILTNWYVRMNRRRLKGENGMEDCVMALETLFSVLLSLCRLMA
  PYTPFLTELMYQNLKLLIDPASIRDKDTLSIHYLMLPHVREELIDKKTENAVSRMQSVIELGRVIRDRKT
  IPIKYPLKEIVVIHQDPEALEDIKSLEKYIIEELNVRKVTLSTDKNKYGIRLRAEPDHMVLGKRLKGAFK
  AVMVAVKQLSNEELEQFQKSGSIVVEGHELHEEDIRLMYTFDQATGGTAQFEAHSDAQALVLLDVTPDQS
  MVDEGMAREVINRIQKLRKKCNLVPTDEITVYYNAKSEGRYLSNVIESHTDFIYATIKAPLKPYPVPTSD
  NILIQEQTQLKGYELEITITRGSCVPGPACAYVNLNICANGTEQGGVLLLENPKGDNQLNLEKLKSVITS
  IFGVKSTGLSVFNGGTELQNQTDLLSLSGRTLCVTAGASLAPASSPSTLLCQYINLQLVNAEPQECLTGT
  VGTLLLENPLGQNGLTHQGLVYEAAKVFGLRSRRLKLFLNETQTQEITEDIPMKTLNMKTVYVSVLPTTA
  DG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001100572
• ORF Size: 3786 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001100572.1, NP_001094042.1
• RefSeq Size: 4494 bp
• RefSeq ORF: 3789 bp
• Locus ID: 306804
• Cytogenetics: 17p14
• MW: 144.3 kDa
• Gene Summary: participates in the carotid neointimal formation [RGD, Feb 2006]