Iars (NM_001100572) Rat Tagged ORF Clone
CAT#: RR206137
- TrueORF®
Iars (Myc-DDK-tagged ORF) - Rat isoleucyl-tRNA synthetase (Iars), (10 ug)
"NM_001100572" in other vectors (1)
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Iars |
Synonyms | Iarsl |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR206137 representing NM_001100572
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTTCAACAAGTTCCGGAAAACATCAGCTTTCCTGCTGAAGAAGAAAAAATCTTACAGTTCTGGTCCA AATACAACTGTTTCCAGGAATGCCTAAAGCAGTCAAAGCTCAGGCCAAAGTTTACCTTCTATGATGGGCC TCCCTTCGCTACTGGACTGCCTCACTACGGACACATACTTGCGGGGACAATTAAAGATATAGTTACAAGA TACGCTCACCAGAGCGGGTTTCACGTCGACAGAAGATTCGGGTGGGATTGTCATGGCTTACCTGTGGAGT ACGAGATTGACAAGACCCTGGGGATCAAAGGGCCAGAGGACGTGGCCAAGATGGGGATCGCAGAGTACAA CAAGCAGTGCCGAGCTATTGTGATGAGGTACTCCGCCGAGTGGAAGTCTACTGTTACCAGGCTTGGCCGA TGGATCGACTTTGACAATGACTACAAAACCCTGTACCCACAATTCATGGAATCAGTCTGGTGGGTCTTCA AGCAGCTGTATGACAAAGGCCTCGTGTACAGAGGCGTGAAAGTCATGCCCTTCTCCACCGCGTGTGGAAC TCCACTCTCCAACTTCGAGTCGAACCAGAATTACAAGGACGTTCAGGACCCTTCAGTATTTGTAACGTTC CCTTTGGAGGAAGATGAAAACACATCCTTAGTTGCTTGGACAACCACTCCGTGGACCCTACCCAGTAACC TTGCGCTATGTGTCAATCCAGAAATACAGTATGTGAAGATCAAAGACGTTTCCAGGGGGAAGTTGTTCAT TTTGATGGAAGCCAGATTGTCGGCCCTCTATAAACAAGAGAGCGACTATGAGATCCTTGAAAGGTTTCCT GGTGCCTCTCTCAAAGGCAAGAAATACAAGCCGTTGTTTGACTACTTTATTAAGTGTAAGGAGAACGGTG CCTTCACCGTGCTTGTTGACCACTATGTGAAGGATGAAGAAGGCACAGGTGTCGTTCACCAGGCTCCTTA CTTTGGCGCTGACGATCATCGAGTCTGTATGGACTTCAACATCATTCAGAAAGACTCAGCCCCTGTTTGT CCTGTGGATGCTTCAGGCTGCTTCACAGAGGAAGTGACACATTTTGTCGGGCAGTATGTGAAGGATGCTG ACAAAAATATCATCAGGATGTTGAAGGAGCAGGGCCGACTGCTCGTGGCAAGCACCTTCACTCACAGCTA CCCTTTCTGCTGGAGGTCGGACACTCCCCTCATTTACAAGTCCGTGCCCAGCTGGTTCGTGCGGGTGGAG CACATGGTGGACCAGCTACTGAAGAACAATGATCTGTGTTACTGGGTCCCAGAGTTTGTACGAGAAAAAC GGTTTGGAAATTGGCTGAAGGAGGCACGTGACTGGGCAATTTCCCGAAACAGATACTGGGGCACCCCCAT CCCACTGTGGGTCAGTGAAGACTTTGAGGAGGTAGTGTGCATCGGGTCAGTGGCAGAACTTGAAGAATTG TCAGGAACAAAAATCTCAGATCTCCACAGAGAGAGCATCGACCACCTGACCATACCATCCCGCTGCGGGA AAGGACCGTTGCGGCGTGTCTCTGAGGTGTTTGACTGTTGGTTCGAGAGTGGAAGTATGCCTTACGCCCA GGTTCATTACCCCTTTGAGAGCAAGCGAGAGTTCGAGGATGCCTTTCCCGCAGACTTCATTGCTGAAGGC ATCGATCAAACCAGAGGATGGTTTTACACCCTGCTAGTGCTAGCCACAGCCCTCTTTGGACAACCACCTT TCAAGAACGTGATTGTGAACGGCCTCATCTTGGCAAGTGATGGCCAAAAAATGAGCAAACGGAAAAAGAA CTACCCAGATCCGGTTTCCATCATCAATAAGTACGGTGCCGACGCCCTCAGGTTATATCTGATCAATTCC CCTGTGGTGAGAGCAGAAAACCTGCGCTTCAAGGAGGAAGGCGTACGGGATGTCCTCAAAGACGTGCTGC TCCCATGGTACAACGCATACCGCTTCTTCATCCAGAACGTCTTTAGGCTGCACAAGGAAGAAGAAGTAGA GTTTCTGTACAACGAGCACACGGTCCGAGAAAGCCCCAACATCACCGACCGGTGGATTCTGTCCTTCATG CAGTCGCTCATTGCGTTCTTTGAGACGGAAATGGCAGCTTACAGGCTGTACACTGTGGTGCCTCGCCTGG TCAAGTTCGTAGATATTCTGACCAACTGGTACGTCCGAATGAACAGGAGAAGATTAAAGGGGGAGAATGG CATGGAAGACTGTGTTATGGCTCTGGAGACTCTGTTCAGTGTCCTGCTTTCTCTGTGCAGACTGATGGCC CCTTACACACCCTTTCTTACTGAACTGATGTACCAGAACCTGAAGCTGCTGATTGACCCTGCATCTATCC GGGACAAGGACACCCTCAGCATCCACTACCTCATGCTGCCTCATGTTCGAGAGGAGCTGATTGACAAGAA AACTGAGAACGCCGTGTCTAGAATGCAGTCCGTGATTGAGCTTGGGAGAGTGATTCGAGACCGGAAAACT ATTCCAATAAAGTATCCCTTGAAGGAAATTGTCGTCATCCACCAAGACCCAGAAGCTCTTGAGGATATCA AGTCCTTGGAGAAGTATATCATTGAGGAACTAAATGTTCGAAAAGTTACACTGTCTACTGATAAAAACAA GTATGGCATTCGACTGAGGGCTGAGCCAGATCACATGGTCCTTGGGAAACGTCTGAAGGGAGCCTTTAAA GCAGTGATGGTGGCCGTAAAGCAGCTGAGCAATGAGGAGCTGGAGCAGTTTCAGAAGAGTGGGAGCATCG TCGTGGAAGGCCATGAATTACACGAAGAAGATATTCGTCTCATGTACACCTTTGACCAGGCCACAGGAGG CACTGCACAGTTTGAAGCACACTCTGATGCTCAGGCTTTAGTTCTCTTAGATGTCACACCTGACCAGTCA ATGGTGGACGAAGGCATGGCTCGGGAAGTCATCAATCGCATCCAGAAGCTTCGCAAGAAGTGCAACCTGG TTCCGACGGATGAAATAACAGTTTACTATAATGCCAAATCTGAAGGAAGATACTTGAGTAATGTCATCGA AAGCCACACTGACTTCATATATGCCACTATCAAAGCTCCCTTGAAGCCCTATCCAGTTCCTACATCAGAT AATATCCTTATTCAAGAGCAAACACAGTTAAAGGGGTACGAGCTGGAAATCACGATCACCAGAGGATCCT GTGTGCCTGGGCCCGCTTGTGCTTATGTGAACCTTAACATTTGTGCAAATGGCACTGAGCAAGGTGGAGT GTTACTCCTGGAGAATCCGAAGGGTGATAATCAGTTGAACCTTGAGAAGCTGAAGAGCGTCATTACCAGC ATATTCGGTGTTAAAAGCACAGGGCTGTCAGTCTTCAACGGTGGAACAGAATTACAGAACCAGACTGACT TGCTGAGCCTCAGTGGAAGGACGCTGTGTGTGACTGCAGGAGCATCCCTGGCTCCAGCCAGCAGCCCCAG CACCCTTCTCTGTCAGTACATCAACCTGCAGCTCGTGAACGCAGAGCCACAAGAGTGTTTAACAGGAACT GTGGGCACCCTCCTGCTTGAAAACCCACTTGGACAGAACGGGCTCACCCACCAAGGGCTTGTGTACGAAG CAGCCAAGGTGTTTGGACTGCGCAGCCGGAGGCTAAAGCTGTTCCTGAATGAGACTCAGACACAAGAAAT TACAGAAGATATCCCAATGAAGACTTTGAATATGAAGACTGTATATGTTTCTGTCTTGCCAACGACTGCA GATGGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR206137 representing NM_001100572
Red=Cloning site Green=Tags(s) MVQQVPENISFPAEEEKILQFWSKYNCFQECLKQSKLRPKFTFYDGPPFATGLPHYGHILAGTIKDIVTR YAHQSGFHVDRRFGWDCHGLPVEYEIDKTLGIKGPEDVAKMGIAEYNKQCRAIVMRYSAEWKSTVTRLGR WIDFDNDYKTLYPQFMESVWWVFKQLYDKGLVYRGVKVMPFSTACGTPLSNFESNQNYKDVQDPSVFVTF PLEEDENTSLVAWTTTPWTLPSNLALCVNPEIQYVKIKDVSRGKLFILMEARLSALYKQESDYEILERFP GASLKGKKYKPLFDYFIKCKENGAFTVLVDHYVKDEEGTGVVHQAPYFGADDHRVCMDFNIIQKDSAPVC PVDASGCFTEEVTHFVGQYVKDADKNIIRMLKEQGRLLVASTFTHSYPFCWRSDTPLIYKSVPSWFVRVE HMVDQLLKNNDLCYWVPEFVREKRFGNWLKEARDWAISRNRYWGTPIPLWVSEDFEEVVCIGSVAELEEL SGTKISDLHRESIDHLTIPSRCGKGPLRRVSEVFDCWFESGSMPYAQVHYPFESKREFEDAFPADFIAEG IDQTRGWFYTLLVLATALFGQPPFKNVIVNGLILASDGQKMSKRKKNYPDPVSIINKYGADALRLYLINS PVVRAENLRFKEEGVRDVLKDVLLPWYNAYRFFIQNVFRLHKEEEVEFLYNEHTVRESPNITDRWILSFM QSLIAFFETEMAAYRLYTVVPRLVKFVDILTNWYVRMNRRRLKGENGMEDCVMALETLFSVLLSLCRLMA PYTPFLTELMYQNLKLLIDPASIRDKDTLSIHYLMLPHVREELIDKKTENAVSRMQSVIELGRVIRDRKT IPIKYPLKEIVVIHQDPEALEDIKSLEKYIIEELNVRKVTLSTDKNKYGIRLRAEPDHMVLGKRLKGAFK AVMVAVKQLSNEELEQFQKSGSIVVEGHELHEEDIRLMYTFDQATGGTAQFEAHSDAQALVLLDVTPDQS MVDEGMAREVINRIQKLRKKCNLVPTDEITVYYNAKSEGRYLSNVIESHTDFIYATIKAPLKPYPVPTSD NILIQEQTQLKGYELEITITRGSCVPGPACAYVNLNICANGTEQGGVLLLENPKGDNQLNLEKLKSVITS IFGVKSTGLSVFNGGTELQNQTDLLSLSGRTLCVTAGASLAPASSPSTLLCQYINLQLVNAEPQECLTGT VGTLLLENPLGQNGLTHQGLVYEAAKVFGLRSRRLKLFLNETQTQEITEDIPMKTLNMKTVYVSVLPTTA DG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001100572 |
ORF Size | 3786 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001100572.1, NP_001094042.1 |
RefSeq Size | 4494 bp |
RefSeq ORF | 3789 bp |
Locus ID | 306804 |
Cytogenetics | 17p14 |
MW | 144.3 kDa |
Gene Summary | participates in the carotid neointimal formation [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN206137 | Iars (untagged ORF) - Rat isoleucyl-tRNA synthetase (Iars), (10 ug) |
USD 1,209.00 |
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