Tex19.1 (NM_001017482) Rat Tagged ORF Clone

CAT#: RR206124

Tex19.1 (Myc-DDK-tagged ORF) - Rat testis expressed gene 19 (Tex19), (10 ug)

Product Images

Circular map for RR206124

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tex19.1
• Synonyms: Tex19; Tex19a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206124 representing NM_001017482
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGTCCCCCAGTCAGTGTTCGCCATGGGGCCAGGGGTATGTCCTGCCTCTATGGAGCCTGGCTGTACC
  AGCTTGTCCATGGAGAACAAATGAAAATGTGCTTTGCTTGCTTCAAGGCAGCTTTCCTGTTGAATAAATT
  CTACCTGGAGATGGGATACTGGGAACAGGAAGAACTTTCAGAGGAAGAGGAGGAAGAGGAAGTGTGGGAT
  GCCGAGCCCATGGAACACTTGTCAGAGTCAGAGTCCTTGGAGTCAGATTCTAAGCAGGATGCAGGGTCTG
  AGCAGGATGCAGGGTCTGAGCCGAATACAAGGTCTGAGCAGGATGCATGGCAGGGTGTGGGGTCCCTTTA
  TGTGCCTCAGAGTGTCTCTGAATATGGAGGGCCTGGGGCCCTGGTACCAACCCCTTCATGGACACAGTGG
  GTAGTATTCTCCATTTCTGTGCCCACTGAGCTTTTGCCTCAGGAAGCCGTACCCCTGGATCTGGGCCCTG
  AGGATGTTGAGTGGACCCAGGCCCTTCCCTGGAGATTAGATGTCCTTTTCCCCTGTTCGCACCGGCTCAT
  CCCTCCTCTGTCTTGGTGGGATATTTTAGATGTGATGCCATCTCTTGGGCAACCTGTATTGTTGGAGTTG
  AGATCCCTCTGGCCTTTGGACCAGTCAGTAGCACAAACCTGGTTGCAAGACCAGAAGTTTGTCCTCTTGT
  TGGATAGCATACACTTTATGTGCCACCTGCTGTCAATGCATGTCTGCTGGGCTGTAAGGACCCAGGTCCA
  GCACTGGCAGGTGTTGCTGAACCCTGGTGAGATGTGGGTGGCCCATTTGAAGAGAGTATTATTTAGACCG
  CGTGGCCTGTACCCCTGGAGCCTGAGCATCCTGAAGAGTTCGGATTTGGGAATGGAGTTAGTCCCTGCTG
  CTTTCTACCTGCGGAAGAAAGGCTTCTGGGTAGGCTCCTTCTTGCCCTGGAACAGTAGCATACCAGAGAC
  CTGGAGCTGGGATCCAGGGGAGAGGCTGTTTATCACAGATGCTACTATTTGTGCTACTAACTACCACTTT
  GCTCGATCCTTCTTCCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206124 representing NM_001017482
  Red=Cloning site Green=Tags(s)
  
  MCPPVSVRHGARGMSCLYGAWLYQLVHGEQMKMCFACFKAAFLLNKFYLEMGYWEQEELSEEEEEEEVWD
  AEPMEHLSESESLESDSKQDAGSEQDAGSEPNTRSEQDAWQGVGSLYVPQSVSEYGGPGALVPTPSWTQW
  VVFSISVPTELLPQEAVPLDLGPEDVEWTQALPWRLDVLFPCSHRLIPPLSWWDILDVMPSLGQPVLLEL
  RSLWPLDQSVAQTWLQDQKFVLLLDSIHFMCHLLSMHVCWAVRTQVQHWQVLLNPGEMWVAHLKRVLFRP
  RGLYPWSLSILKSSDLGMELVPAAFYLRKKGFWVGSFLPWNSSIPETWSWDPGERLFITDATICATNYHF
  ARSFFP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001017482
• ORF Size: 1068 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001017482.1, NP_001017482.1
• RefSeq Size: 1755 bp
• RefSeq ORF: 1071 bp
• Locus ID: 498033
• UniProt ID: Q5XHY3
• Cytogenetics: 10q32.3
• MW: 40.9 kDa
• Gene Summary: Required during spermatogenesis and placenta development, participating in the repression of retrotransposable elements and prevent their mobilization. Collaborates with the Piwi-interacting RNA (piRNA) pathway, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins. Interacts with Piwi proteins and directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Also during spermatogenesis, promotes, with UBR2, SPO11-dependent recombination foci to accumulate and drive robust homologous chromosome synapsis. Interacts with LINE-1 retrotransposon encoded LIRE1, stimulates LIRE1 polyubiquitination, mediated by UBR2, and degradation, inhibiting LINE-1 retranstoposon mobilization.[UniProtKB/Swiss-Prot Function]