Grm8 (NM_022202) Rat Tagged ORF Clone
CAT#: RR205742
- TrueORF®
Grm8 (Myc-DDK-tagged ORF) - Rat glutamate receptor, metabotropic 8 (Grm8), (10 ug)
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_022202" in other vectors (3)
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Grm8 |
Synonyms | Glur8; Gprc1h; mGlur; Mglur8; mGluR8b |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR205742 representing NM_022202
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTATGCGAGGGAAAGCGCTTAGCCTCTTGCCCTTGCTTCTTCCTCTTGACTGCCAAGTTCTACTGGA TCCTCACAATGATGCAAAGAACTCACAGCCAAGAGTATGCCCATTCCATCCGGGTGGATGGGGACATCAT TTTGGGGGGTCTTTTTCCTGTTCATGCCAAGGGAGAAAGAGGGGTGCCTTGTGGGGAGCTGAAGAAGGAA AAGGGGATCCACAGACTTGAGGCCATGCTTTATGCAATCGACCAGATTAACAAAGACCCTGATCTTCTCT CCAATATCACTCTGGGGGTCCGGATCCTTGACACGTGTTCCAGGGACACCTACGCTTTGGAGCAGTCACT GACCTTCGTGCAGGCACTGATAGAGAAAGATGCGTCTGATGTGAAGTGTGCTAATGGAGATCCGCCCATA TTCACCAAGCCCGACAAGATTTCTGGCGTCATAGGTGCTGCAGCAAGCTCTGTGTCCATCATGGTGGCTA ACATTTTAAGACTATTTAAGATACCTCAGATTAGCTATGCATCCACAGCCCCAGAGCTAAGTGACAACAC CAGGTATGATTTCTTTTCTCGGGTGGTCCCGCCTGACTCCTACCAAGCCCAAGCCATGGTGGACATTGTG ACGGCCCTGGGATGGAATTATGTGTCAACACTGGCTTCCGAGGGGAACTATGGAGAGAGTGGTGTTGAGG CCTTCACTCAGATCTCAAGGGAGATTGGTGGTGTTTGCATTGCTCAATCACAGAAAATCCCACGTGAACC AAGACCTGGGGAATTTGAAAAAATTATCAAACGCCTGCTGGAGACACCTAACGCTCGCGCAGTGATTATG TTTGCCAACGAGGATGACATCAGGAGGATATTGGAAGCAGCAAAAAAATTAAACCAGAGTGGGCATTTTC TATGGATTGGCTCTGATAGTTGGGGATCCAAAATTGCGCCCGTCTACCAGCAGGAGGAGATCGCAGAAGG AGCGGTGACAATTTTGCCTAAAAGAGCATCAATTGATGGGTTTGATCGATACTTTAGAAGCCGAACTCTT GCTAATAATCGAAGAAATGTGTGGTTTGCAGAATTTTGGGAGGAGAATTTTGGATGCAAATTAGGATCAC ATGGGAAGAGGAACAGTCACATAAAGAAATGCACAGGGCTGGAGCGAATTGCACGGGATTCATCTTATGA ACAAGAAGGAAAGGTTCAATTTGTTATTGATGCAGTGTATTCCATGGCTTATGCACTGCATAACATGCAC AAAGAGCGCTGTCCTGGATACATAGGTCTTTGCCCAAGAATGGTCACCATCGATGGGAAAGAGCTACTGG GTTACATCAGGGCCGTGAATTTTAATGGCAGTGCTGGTACACCTGTCACTTTTAATGAAAATGGAGATGC TCCTGGACGCTACGATATCTTCCAGTATCAGATAAACAACAAAAGTACAGAGTACAAAATCATCGGGCAC TGGACAAATCAACTTCATCTAAAGGTGGAAGATATGCAGTGGGCTAATAGAGAGCACACGCACCCAGCAT CTGTCTGCAGCCTGCCGTGCAAGCCCGGGGAGAGGAAGAAAACCGTGAAAGGGGTCCCTTGCTGCTGGCA CTGTGAACGTTGTGAGGGTTATAACTACCAGGTGGATGAACTGTCCTGTGAACTTTGCCCTTTGGATCAG AGACCAAACATCAACCGCACAGGCTGCCAGAGGATCCCCATCATCAAGTTGGAGTGGCATTCGCCCTGGG CTGTGGTACCTGTGTTCATAGCAATATTGGGAATCATTGCTACCACCTTTGTGATTGTGACCTTTGTCCG CTATAATGACACGCCCATCGTGAGAGCTTCTGGGCGGGAACTTAGTTACGTGCTCCTAACGGGGATTTTT CTCTGTTACTCCATCACCTTTTTGATGATTGCCGCACCTGACACGATCATCTGCTCCTTCCGACGGATCT TCTTGGGACTTGGTATGTGTTTCAGCTATGCAGCACTTTTGACCAAAACAAACCGTATCCACCGAATATT CGAGCAAGGGAAGAAATCTGTCACAGCACCTAAGTTTATCAGCCCAGCATCCCAACTGGTGATCACCTTC AGCCTCATCTCTGTCCAGCTCCTCGGAGTGTTTGTGTGGTTTGTCGTGGATCCCCCCCACACCATCATTG ACTATGGCGAACAGCGAACGCTGGATCCTGAGAACGCCAGGGGAGTGCTCAAGTGTGATATTTCGGATCT GTCACTCATTTGTTCCCTGGGATACAGTATCCTCCTGATGGTCACCTGTACTGTTTATGCCATTAAAACC AGAGGGGTCCCAGAGACGTTCAATGAAGCCAAACCTATTGGCTTTACAATGTACACCACGTGCATCATTT GGTTAGCTTTCATCCCCATCTTTTTTGGTACAGCCCAGTCAGCAGAAAAGATGTACATCCAGACAACGAC ACTTACTGTTTCCATGAGTTTAAGTGCTTCAGTGTCTCTGGGAATGCTCTATATGCCCAAAGTTTATATT ATAATTTTTCATCCAGAGCAGAATGTTCAAAAACGCAAGAGAAGCTTCAAGGCTGTGGTCACAGCTGCTA CCATGCAAAGCAAACTGATCCAAAAGGGAAATGACAGACCAAATGGCGAGGTGAAAAGTGAACTCTGTGA GAGTCTTGAAACCAACACGTCTTCTACCAAGACAACATACATCAGCTACAGTAATCATTCGATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR205742 representing NM_022202
Red=Cloning site Green=Tags(s) MVCEGKRLASCPCFFLLTAKFYWILTMMQRTHSQEYAHSIRVDGDIILGGLFPVHAKGERGVPCGELKKE KGIHRLEAMLYAIDQINKDPDLLSNITLGVRILDTCSRDTYALEQSLTFVQALIEKDASDVKCANGDPPI FTKPDKISGVIGAAASSVSIMVANILRLFKIPQISYASTAPELSDNTRYDFFSRVVPPDSYQAQAMVDIV TALGWNYVSTLASEGNYGESGVEAFTQISREIGGVCIAQSQKIPREPRPGEFEKIIKRLLETPNARAVIM FANEDDIRRILEAAKKLNQSGHFLWIGSDSWGSKIAPVYQQEEIAEGAVTILPKRASIDGFDRYFRSRTL ANNRRNVWFAEFWEENFGCKLGSHGKRNSHIKKCTGLERIARDSSYEQEGKVQFVIDAVYSMAYALHNMH KERCPGYIGLCPRMVTIDGKELLGYIRAVNFNGSAGTPVTFNENGDAPGRYDIFQYQINNKSTEYKIIGH WTNQLHLKVEDMQWANREHTHPASVCSLPCKPGERKKTVKGVPCCWHCERCEGYNYQVDELSCELCPLDQ RPNINRTGCQRIPIIKLEWHSPWAVVPVFIAILGIIATTFVIVTFVRYNDTPIVRASGRELSYVLLTGIF LCYSITFLMIAAPDTIICSFRRIFLGLGMCFSYAALLTKTNRIHRIFEQGKKSVTAPKFISPASQLVITF SLISVQLLGVFVWFVVDPPHTIIDYGEQRTLDPENARGVLKCDISDLSLICSLGYSILLMVTCTVYAIKT RGVPETFNEAKPIGFTMYTTCIIWLAFIPIFFGTAQSAEKMYIQTTTLTVSMSLSASVSLGMLYMPKVYI IIFHPEQNVQKRKRSFKAVVTAATMQSKLIQKGNDRPNGEVKSELCESLETNTSSTKTTYISYSNHSI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_022202 |
ORF Size | 2724 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_022202.1, NP_071538.1 |
RefSeq Size | 2793 bp |
RefSeq ORF | 2727 bp |
Locus ID | 60590 |
UniProt ID | P70579 |
Cytogenetics | 4q22 |
MW | 101.9 kDa |
Gene Summary | metabotropic glutamate receptor that may be involved in synaptic transmission [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN205742 | Grm8 (untagged ORF) - Rat glutamate receptor, metabotropic 8 (Grm8), (10 ug) |
USD 915.00 |
|
RR205742L3 | Lenti ORF clone of Grm8 (Myc-DDK-tagged ORF) - Rat glutamate receptor, metabotropic 8 (Grm8), (10 ug) |
USD 1,214.00 |
|
RR205742L4 | Lenti ORF clone of Grm8 (mGFP-tagged ORF) - Rat glutamate receptor, metabotropic 8 (Grm8), (10 ug) |
USD 1,214.00 |
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