Flii (NM_001008279) Rat Tagged ORF Clone

CAT#: RR205540

Flii (Myc-DDK-tagged ORF) - Rat flightless I homolog (Drosophila) (Flii), (10 ug)

Product Images

Circular map for RR205540

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Flii
• Synonyms: Fliih
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR205540 representing NM_001008279
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCCACCGGGGTGCTGCCGTTCGTGCGCGGCGTGGACCTCAGCGGCAACGACTTCAAGGGCGGCT
  ACTTCCCTGAGAATGTCAAGGCTATGACCAGCCTGCGATGGCTAAAGCTGAACCGCACAGGTCTCTGCTA
  CCTGCCTGAAGAACTAGCAGCTCTGCAGAAGCTGGAGCACCTGTCTGTGAGCCACAACAATCTGACCACG
  CTTCATGGGGAACTGTCCAGCCTGCCTTCACTTCGGGCCATTGTAGCTCGAGCCAACAGCCTGAAGAATT
  CTGGAGTCCCCGATGATATCTTCAAGCTGGATGATCTCTCCGTTCTGGACTTGAGCCATAACCAGTTGAC
  AGAATGCCCACGAGAGCTGGAGAACGCCAAGAACATGCTGGTGCTCAACCTCAGCCATAACAGCATTGAC
  TCCATCCCCAACCAGCTGTTCATCAACCTTACGGACTTGCTGTACCTGGACCTCAGTGAGAACCGCCTAG
  AGAGCCTTCCCCCTCAAATGCGCCGCCTCGTGCACCTTCAGACACTGGTGCTGAATGGAAACCCGCTGCT
  GCATGCACAGCTCCGGCAGCTCCCGGCCATGATGGCCCTACAAACCCTGCATCTGAGGAACACTCAACGC
  ACCCAGAGCAACCTCCCCACTAGCCTGGAGGGCCTGAGCAACCTTTCAGACGTGGACCTGTCGTGCAATG
  ACTTGACAAGGGTGCCAGAGTGCCTGTACACCCTCCCCAACCTGCACCGCCTCAACCTCAGTAGCAACCA
  GATCGCAGAGCTATCTCTGTGCATCGACCAGTGGGTGCACCTGGAGACCTTGAATCTGTCCCGCAACCAG
  CTCACCTCCCTGCCTTCAGCCATTTGCAAGCTCACGAAGCTGAAGAAATTGTACCTGAACTCCAACAAGC
  TGGACTTTGATGGGCTACCTTCTGGCATCGGCAAGCTCACCAGCCTGGAGGAATTCATGGCTGCTAACAA
  CAACCTAGAACTGATTCCAGAAAGTCTCTGCAGGTGCCCAAAGCTGAGGAAACTCGTCCTGAACAAGAAC
  CGCCTGGTGACCCTTCCTGAGGCCATCCACTTTCTGACAGAGATCGAGGTCCTGGATGTTCGAGAAAACC
  CCAGCTTGGTCATGCCGCCCAAGCCTGCTGACCACACCGCCGAGTGGTACAACATTGACTTCTCACTACA
  GAACCAGCTGAGGCTGGCGGGTGCCTCCCCGGCCACAGTGGCCGCCGCTGCAGCTGTAGGGAGCGGGTCC
  AAGGACCCCTTGGCTCGCAAGATGCGGCTACGGAGGCGCAAGGACTCGGCACAGGATGTTCAGGCCAAGC
  AAGTGCTAAAGGGCATGTCAGACGTTGCCCAGGAGAAAAACAAAAACCAAGAGGAAAGCACAGATGCCCG
  AGCACCTGGGGGAAAGGTACGGCGCTGGGACCAGGGCCTGGAGAAGCCACGCCTTGACTACTCGGAGTTC
  TTTACGGAGGATGTGGGCCAGCTGCCTGGGTTGACCATCTGGCAAATTGAGAACTTTGTGCCTGTGCTGG
  TGGAGGAAGCCTTCCATGGCAAGTTCTACGAGGCCGACTGCTACATTGTGCTCAAGACCTTCCTGGATGA
  CAGCGGCTCCCTGAACTGGGAGATCTACTACTGGATTGGCGGGGAGGCCACACTTGACAAGAAAGCCTGT
  TCTGCCATTCATGCTGTCAACCTGCGCAACTATCTGGGCGCTGAGTGCCGTACTGCGCGGGAGGAGATGG
  GCGATGAGAGTGAGGGCTTCCTGCAGGTGTTTGACAATGACATCTCCTACATTGAAGGCGGAACAGCCAG
  TGGCTTCTATACTGTGGAAGACACACACTATGTTACCAGGATGTACCGTGTGTATGGGAAAAAGAACATC
  AAATTGGAGCCTGTGCCCCTCAAGGGGACCTCACTGGATCCGAGGTTTGTGTTTCTCCTGGACCAAGGAC
  TGGACATTTATGTATGGAGAGGGGCCCAGGCCACACTGAGCAACACCACAAAGGCCAGACTCTTTGCAGA
  GAAAATGAACAAGAATGAGCGGAAAGGGAAGGCGGAAATCACGCTCCTGGTGCAGGGTCAGGAGCCACCG
  GAGTTCTGGGATGTGCTGGGCGGGGAACCCTCTGAGATAAAAAAGCATGTGCCTGATGACTTCTGGCCGC
  CCCAGCCCAAGCTATACAAGGTGGGCCTGGGGCTGGGCTACTTGGAGCTTCCGCAGATCAACTACAAGCT
  CTCAGTGGAACACAAAAAACGGCCCAAGGTGGAGCTGATGCCAGGAATGAGGCTGTTGCAGAGCCTGCTG
  GACACACGATGTGTGTACATCCTGGACTGCTGGTCTGATGTATTCATCTGGCTTGGCCGCAAGTCCCCAC
  GCCTAGTGCGTGCCGCTGCACTCAAACTGGGCCAGGAGCTGTGTGGGATGCTACACCGGCCACGACATAC
  AGTGGTCAGCCGCAGCCTGGAGGGCACCGAGGCACAGGTGTTCAAGGCCAAGTTCAAAAACTGGGATGAT
  GTGTTGACAGTGGACTACACACGAAATGCAGAGGCTGTGCTGCAGGGCCCGGGGATCTCTGGGAAGGTGA
  AGCGAGACACGGAGAAGAAAGACCAGATGAAGGCTGACCTCACTGCACTCTTCCTACCTCGGCAGCCACC
  AATGCCACTGGCTGAGGCCGAGCAGCTGATGGAGGAGTGGAACGAAGACCTGGATGGCATGGAGGGCTTC
  GTGCTGGAGGGCCGGAAGTTCACTCGACTGGCAGAGGAGGAGTTTGGCCACTTCTACACACAGGACTGCT
  ATGTCTTCCTCTGCAGGTACTGGGTGCCTGTGGAGTACGAGGAGGAAGAGAAGCCAGAAGACAAGGAAGG
  GAAGGCCTCGGCAGAGGGCAGAGAAGAGGAGGAAGCAGCAGCTGAGACAGAGGAGAAGCAGCCAGAGGAG
  GATTTTCAGTGCATCGTTTACTTCTGGCAGGGCCGGGAGGCCTCCAACATGGGCTGGCTTACCTTCACAT
  TCAGTCTGCAGAAGAAGTTCGAGAGCCTTTTCCCTGGCAAGCTAGAGGTAGTACGCATGACACAACAACA
  GGAGAACCCTAAGTTTCTATCCCACTTCAAAAGAAAGTTCATCATCCACCGGGGCAAGAGGAAGGTGACC
  CAGACTCTCCAGCCGACCCTCTATCAGATCCGGACTAATGGCAGTGCCCTCTGCACCCGGTGCATTCAGA
  TCAACACTGACTCCAGTCTTCTCAACTCTGAGTTCTGCTTCATCCTCAAGGTCCCCTTTGAAAGCGAGGA
  CAACCAAGGCATCGTGTATGCCTGGGTAGGCCGGGCATCAGACCCTGACGAAGCCAAGCTGGCAGAGGAC
  ATCCTGAACACCATGTTTGACCCCTCCTACAGCAAACAGGTCATCAACGAGGGTGAGGAGCCAGAGAACT
  TCTTCTGGGTAGGCATCGGTGCACAGAAACCCTATGACGATGACGCAGAGTACATGAAGCACACGAGGCT
  CTTCAGGTGCTCCAATGAGAAGGGCTACTTCGCAGTGACTGAGAAATGCTCTGACTTTTGCCAAGATGAC
  CTGGCAGACGATGACATCATGCTGCTAGACAATGGCCAAGAAGTGTACATGTGGGTTGGGACTCAGACAA
  GCCAAGTGGAGATCAAACTGAGTCTGAAGGCTTGCCAGGTATACATCCAGCACACACGCTCTAAAGAACA
  TGAGCGGCCACGCCGCCTGCGCCTGGTCCGCAAAGGTAACGAACAACGTGCCTTCACCCGCTGCTTCCAT
  GCCTGGAGCACGTTCCGTCAAGCCCCAGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR205540 representing NM_001008279
  Red=Cloning site Green=Tags(s)
  
  MEATGVLPFVRGVDLSGNDFKGGYFPENVKAMTSLRWLKLNRTGLCYLPEELAALQKLEHLSVSHNNLTT
  LHGELSSLPSLRAIVARANSLKNSGVPDDIFKLDDLSVLDLSHNQLTECPRELENAKNMLVLNLSHNSID
  SIPNQLFINLTDLLYLDLSENRLESLPPQMRRLVHLQTLVLNGNPLLHAQLRQLPAMMALQTLHLRNTQR
  TQSNLPTSLEGLSNLSDVDLSCNDLTRVPECLYTLPNLHRLNLSSNQIAELSLCIDQWVHLETLNLSRNQ
  LTSLPSAICKLTKLKKLYLNSNKLDFDGLPSGIGKLTSLEEFMAANNNLELIPESLCRCPKLRKLVLNKN
  RLVTLPEAIHFLTEIEVLDVRENPSLVMPPKPADHTAEWYNIDFSLQNQLRLAGASPATVAAAAAVGSGS
  KDPLARKMRLRRRKDSAQDVQAKQVLKGMSDVAQEKNKNQEESTDARAPGGKVRRWDQGLEKPRLDYSEF
  FTEDVGQLPGLTIWQIENFVPVLVEEAFHGKFYEADCYIVLKTFLDDSGSLNWEIYYWIGGEATLDKKAC
  SAIHAVNLRNYLGAECRTAREEMGDESEGFLQVFDNDISYIEGGTASGFYTVEDTHYVTRMYRVYGKKNI
  KLEPVPLKGTSLDPRFVFLLDQGLDIYVWRGAQATLSNTTKARLFAEKMNKNERKGKAEITLLVQGQEPP
  EFWDVLGGEPSEIKKHVPDDFWPPQPKLYKVGLGLGYLELPQINYKLSVEHKKRPKVELMPGMRLLQSLL
  DTRCVYILDCWSDVFIWLGRKSPRLVRAAALKLGQELCGMLHRPRHTVVSRSLEGTEAQVFKAKFKNWDD
  VLTVDYTRNAEAVLQGPGISGKVKRDTEKKDQMKADLTALFLPRQPPMPLAEAEQLMEEWNEDLDGMEGF
  VLEGRKFTRLAEEEFGHFYTQDCYVFLCRYWVPVEYEEEEKPEDKEGKASAEGREEEEAAAETEEKQPEE
  DFQCIVYFWQGREASNMGWLTFTFSLQKKFESLFPGKLEVVRMTQQQENPKFLSHFKRKFIIHRGKRKVT
  QTLQPTLYQIRTNGSALCTRCIQINTDSSLLNSEFCFILKVPFESEDNQGIVYAWVGRASDPDEAKLAED
  ILNTMFDPSYSKQVINEGEEPENFFWVGIGAQKPYDDDAEYMKHTRLFRCSNEKGYFAVTEKCSDFCQDD
  LADDDIMLLDNGQEVYMWVGTQTSQVEIKLSLKACQVYIQHTRSKEHERPRRLRLVRKGNEQRAFTRCFH
  AWSTFRQAPA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001008279
• ORF Size: 3810 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001008279.1, NP_001008280.1
• RefSeq Size: 4035 bp
• RefSeq ORF: 3813 bp
• Locus ID: 287375
• Cytogenetics: 10q22
• MW: 144.9 kDa