Ankle2 (NM_001047901) Rat Tagged ORF Clone

CAT#: RR204756

Ankle2 (Myc-DDK-tagged ORF) - Rat ankyrin repeat and LEM domain containing 2 (Ankle2), (10 ug)

Product Images

Circular map for RR204756

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ankle2
• Synonyms: Ab2-034; RGD1310191
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR204756 representing NM_001047901
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGTGGCAGCGGTTGGCTGTGGTGGAGTGGGCGGCCCTGGCCTGGGAGCTCCTGGGCGCCTCGGTGC
  TGCTCATCGCCGTGCGCTGGCTGGTCCGGCGCCTGGAGAATCTTTCTCGGGACCCGAACCGCTGCGGGAC
  ACTCTCCTCTCTGCCTGGCGCGTCTGCAGCGGTGTCCGCTCAGCCCGGTGAAGTGATGACAATGGACGCT
  ATGTTGGCTCGACTGAAACTCTTGAATCCAGATGACCTCAGACAAGAAGTTATGAAAGCTGGACTGAAAT
  GTGGGCCCATTACATCAACTACAAGATTTATTTTTGAGAAGAAATTAGCGCAGGCCTTACTAGAACAAGG
  TGGACTGCTGACTTCCTCTCTTCCAAAGCACAGTGAGGTGACTGGCACGGCATTTATCCATGGCACTTCA
  AGGACTCCTGCATTTGTGGACAGGAAACAAACTCAGCAGGCCTGTTTATCTGAAGACAGAGATTTTGGTT
  ATAGTGTGGGCTTGAACCCTCCAGAAGAGGAAGCTGTAACATCCAGTGTGCACCCCATACCCTTTAGTGC
  CTCCACCAGGAATGACAACCACAAGGCTGGAGTGACAACTGCTAAGGAGCCCCTTCTGTACTATGGAGTG
  TGTCCAGTGTATGAGGATGGCCTTGTGAGACACGAAAGGATCCATGTTTACGAAGATAAAAAGGAAGCCT
  TACAAGCTGTCAAATTGATCAAAGGGTCCCGGTTTAAAGCTTTTCCAACAAGAGAAGATGCTGAGAAATT
  TGCTAGAGGGATATGTGATTACTTACCTTCTCCCAGCAAAAATATGTCCTTACTGTCTCCTGTAAAGGCA
  ATGCCCCTCTGCAGCAATGATGGGCCAAAAGCAGATGGCTTGTGCCTGGCTGAATCAGAAACAGTAAACA
  AAGAACGAGCAAACAGTTATAAAAACCCCCGTACACAGGACCTTACTGCCAAGCTGCGGAAAGCTGTTGA
  AAGAGGGGAGGAACACACCTTTTCTGATCTTATCTGGAGTAATCCTCGATATCTAATTGGTTCTGGGGAC
  AACCCAACCATTGTGCAAGAAGGATGTAGGTACAATGTCATGCATGTTGCTGCCAAAGAGAATCAGGCGT
  CTGTGTGCCAGCTGACATTAGAGACTCTGGAGAACCCTGAATTTATGCGTCTTATGTACCCAGATGATAA
  CATGGACATGCTACAGAAACGTATCCTCTACATTGTCGACCTGTATCTTAATACTCCTGACAAAGTGGGC
  TTTGACACACCATTGCATTTTGCTTGTAAATTTGGGAATGTGGATGTAGTCAACGTGCTTTCTTCCCACC
  CTTTGATTGTGAAAAACCCAAGGAATAAATATGGTAAAACACCTGAAGACGTAATTTGTGAAAGAAGCAA
  AAACAAATCTGTAGAACTGAGAGAAAGGATTAGAGAATACTTAATGGGTCACTACTATGTACCACTTCTG
  AGAGCCGAAGACACCTCTCCTGTCATTGGGGAGCTGTGGTCCTCAGACCAGAAAGCTGAAGCATCCAGTG
  CCGACCACTGCAGAAGTAGCCCCAGGGATCCTGTGATGACACTGAGAGCTTTTGTGGGGCCTCTGAGTCC
  ATCCAAAGCAGAAGATTTTCGAAAGCTTTGGAAAACTCCACCTCGAAAAAAAGCAGGCCTCTTCCACAGC
  ATCAGGAAGTCTGACCCAGAAAGAGGCATTGAGAGAGTTGGAAGGGAGCTAGCTCATGAGCTGGGGTATC
  CTTGGGTTGAATACTGGGAATTTCTGGGATGTTTTGTTGATCTGTCTTCCCAGGAAGGCTTGCAAAGACT
  AGAAGAATACCTTATCCAGAAGGAGTTAAACCAAAAGGCTCAACATGAAATAGGAGAAGAGGAAGGCTGC
  CTTCAGGACAGAACCTCAGATTTTGGCAGTGGAAAGAAGTACAGCAACTCCATCTCCGTGGGAGCGTTTC
  TAGATGGGGGTGATGACATTAGCTTGGAAGAAGTTAAAAATCAGCAAAATACAGTTCCAAGTCAGAGTCA
  GCCCTCAGTCGACAAATTCCAAAACTCCAAAAGTGGTTCCCCTTCCTTGGGACAGAAGGCGGACCCTGGA
  GAAGCCGCAGTTGGATTTTACCCAGATAACAGCAGAAATGGGTTTTGTTATCCTCTGAACAGCAGGACTG
  CAGATGGAAGGGAAACAGAGGCCACCAATGGAGAGGAGGCCCTTCCACCACCTGTTTCTATTTTGACTCA
  AGAGTTTGATAAACTGAATTTGCAAAGTCTAGGAGATAACCTTTTCGAGACACCAAATAAAAATAGGGAA
  CTAGAGGATAAGATACTGGCTTCAAGTAAAGGTGTAGTAGAAAGTGGCTTGGCATCTCCTGCTGCAATAG
  CAAGACTTGAAAATAAGCAAGTCAGGACCAACAGTGAGGTGTCAGAGGCAATGGCTGAAATGTCTTTGGG
  CCCCAACAGCCCCCAACTTGGGGTTCAGGCTGGTCTGGAACCTGGGTTCTCATCTGCCACAGCAGATCCC
  ACCAAGAGGCTCTTCCTGTCTGGAGAGGAGCCATCAAAACTAGATCGAGATGTTTTAGCAGCCCTTGAGT
  GTGCGATTATTGACCCTGGTCTGTACCCAGCAGTCCACAGATGGAAAAGTACCGTCATGGGCTACTCGCC
  CTCAGACAGACAGAGAGGAGGATACAATGTTGAAAATAAGTGCTTGCAGCTCTATGGCAGGAACCAACAA
  GAGATGCTACTGAATAGTCCATCTTCACTGCTTCTGGAGGAAATGGTTAAGGGTTTTGTCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR204756 representing NM_001047901
  Red=Cloning site Green=Tags(s)
  
  MLWQRLAVVEWAALAWELLGASVLLIAVRWLVRRLENLSRDPNRCGTLSSLPGASAAVSAQPGEVMTMDA
  MLARLKLLNPDDLRQEVMKAGLKCGPITSTTRFIFEKKLAQALLEQGGLLTSSLPKHSEVTGTAFIHGTS
  RTPAFVDRKQTQQACLSEDRDFGYSVGLNPPEEEAVTSSVHPIPFSASTRNDNHKAGVTTAKEPLLYYGV
  CPVYEDGLVRHERIHVYEDKKEALQAVKLIKGSRFKAFPTREDAEKFARGICDYLPSPSKNMSLLSPVKA
  MPLCSNDGPKADGLCLAESETVNKERANSYKNPRTQDLTAKLRKAVERGEEHTFSDLIWSNPRYLIGSGD
  NPTIVQEGCRYNVMHVAAKENQASVCQLTLETLENPEFMRLMYPDDNMDMLQKRILYIVDLYLNTPDKVG
  FDTPLHFACKFGNVDVVNVLSSHPLIVKNPRNKYGKTPEDVICERSKNKSVELRERIREYLMGHYYVPLL
  RAEDTSPVIGELWSSDQKAEASSADHCRSSPRDPVMTLRAFVGPLSPSKAEDFRKLWKTPPRKKAGLFHS
  IRKSDPERGIERVGRELAHELGYPWVEYWEFLGCFVDLSSQEGLQRLEEYLIQKELNQKAQHEIGEEEGC
  LQDRTSDFGSGKKYSNSISVGAFLDGGDDISLEEVKNQQNTVPSQSQPSVDKFQNSKSGSPSLGQKADPG
  EAAVGFYPDNSRNGFCYPLNSRTADGRETEATNGEEALPPPVSILTQEFDKLNLQSLGDNLFETPNKNRE
  LEDKILASSKGVVESGLASPAAIARLENKQVRTNSEVSEAMAEMSLGPNSPQLGVQAGLEPGFSSATADP
  TKRLFLSGEEPSKLDRDVLAALECAIIDPGLYPAVHRWKSTVMGYSPSDRQRGGYNVENKCLQLYGRNQQ
  EMLLNSPSSLLLEEMVKGFVF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001047901
• ORF Size: 2793 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001047901.1, NP_001041366.1
• RefSeq Size: 2842 bp
• RefSeq ORF: 2796 bp
• Locus ID: 360829
• UniProt ID: Q7TP65
• Cytogenetics: 12q16
• MW: 103.4 kDa
• Gene Summary: Involved in mitotic nuclear envelope reassembly by promoting dephosphorylation of BAF/BANF1 during mitotic exit. Coordinates the control of BAF/BANF1 dephosphorylation by inhibiting VRK1 kinase and promoting dephosphorylation of BAF/BANF1 by protein phosphatase 2A (PP2A), thereby facilitating nuclear envelope assembly. It is unclear whether it acts as a real PP2A regulatory subunit or whether it is involved in recruitment of the PP2A complex (By similarity).[UniProtKB/Swiss-Prot Function]