Aars2 (NM_001106891) Rat Tagged ORF Clone

CAT#: RR204715

Aars2 (Myc-DDK-tagged ORF) - Rat alanyl-tRNA synthetase 2, mitochondrial (putative) (Aars2), nuclear gene encoding mitochondrial protein, (10 ug)

Product Images

Circular map for RR204715

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Aars2
• Synonyms: Aarsl; AlaRS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR204715 representing NM_001106891
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGTCAGTGGCTGCTGCGGCCGGGAGGCTGCGGCGAGCCATTGGGAGGTCGTGCCCATGGCAGC
  GGTTCTCAACCGAGCCCCACCCACCCCACGGAGCGGCCGTGCGGGACGCCTTCCTGAGCTTCTTCCGAGA
  TCGCCACGGCCACCGGCTCGTGCCCTCCGCTTCCGTGAGGCCGCGCGGCGACCCCAGCCTGCTTTTCGTC
  AACGCAGGCATGAACCAGTTCAAGCCCATCTTCCTGGGCACGGTGGATCCACGAAGCGAGATGGCAGGCT
  TCCGACGAGTAGCTAACAGCCAGAAATGTGTCAGGGCTGGAGGACGCCATAACGACCTGGAAGATGTGGG
  CCGTGATCTCTCTCATCATACTTTTTTTGAGATGCTTGGCAACTGGGCTTTTGGGGGTGAATATTTTAAG
  AAAGAAGCATGTAGCATGGCCTGGGAACTGCTGACTCAAGTCTATGGGATCCCTGAGGACAGGTTATGGG
  TCTCCTATTTCAGTGGTGACTCCAAGACAGGGTTGGACCCAGACCTGGAGACCAGAGACATCTGGCTCAG
  CTTGGGGGTACCTGCCAGCCGTGTGCTCTCGTTCGGACTGCAAGAGAACTTCTGGGAGATGGGAGACACC
  GGACCTTGTGGGCCTTGTACCGAGATCCATTACGATCTGGCTGGTGGAATGGGACCCCCCCAGCTGGTTG
  AGCTATGGAATCTGGTCTTCATGCAACACTACAGAGAGGCAGACGGAAGCCTGCATCTGCTGCCACAGCA
  ACATGTGGACACAGGAATGGGCCTGGAAAGACTGGTGGCTGTACTGCAGGGCAAGCATTCCACCTACGAC
  ACCGACCTCTTTTCTCCACTGCTTGATGCTATACACCAGAGCTGCAGGGTTCCCCCGTACTCTGGCCGCG
  TAGGGGCAGCGGATGAGGGACGAATAGACACAGCGTACCGAGTTGTAGCTGATCACATCCGCACGCTCAG
  TGTCTGCATTGCTGATGGCGTCTCCCCGGGGATGTCAGGCGCCCCGCTAGTTCTTCGTCGGATTCTCCGT
  CGAGCTGTGCGCTATTCCACAGAGGTCTTGCAGGCACCACCTGGCTTTCTGGGCAACCTGGTACCAGTGG
  TGGTGGCGACATTGGGAGCTGCTTACCCAGAACTTCAGAAGAACTCAGTCAAGGTATTGATCTGGGAGAT
  AGCCAACCTGGTGTCAGAGGATGAGGCAGCCTTCCTGGCCTCCCTGCAGAGAGGCCGTCGGATCATTGAT
  CGCACCGTTAAGCGCTTGGGCCCTTCTGATTTGTTCCCTGCCGAAGTGGCCTGGTCACTGTCACTGTCTG
  GGAATCTGGGGATCCCCCTGGACCTGGTGCAGCTAATGTTGGAGGAGAAGGGGGTGAAGCTGGACACTGC
  GGGCCTGGAGCAGCTAGCCCAGAAGGAAGCCCAGCACCGGGCCCAGCAGGCAGAGGCAGCTCAGGAGGAG
  GGATTGTGTCTTGATGTCCACGCACTGGAGGAGCTGCACCGTCAAGGCATACCCACAACTGATGACAGCC
  CCAAATATAACTACTCTCTTCGCCCCAATGGGGATTATGAGTTTGGCCTCTGTGAGGCCCAGGTATTACA
  GCTGTATTCAGAGACTGGGACAGCTGTGGCCTCCGTGGGAGAAGGCCAGCGCTGTGGCCTCCTATTGGAC
  AGAACCAACTTCTATGCTGAACAAGGGGGTCAGGCTTCGGACCGAGGCTACCTCATTCGTACAGGGCAGC
  AGGATGTGTTGTTCCCTGTGGCCCGGGCTCAGGTCTGTGGAGGCTTCATCCTGCATGAGGCCATGGCTCC
  CGAGTGCTTACAAGTCGGGGACCGAGTGCAGCTCTATGTGGATAAGGCCTGGCGAATGGGATGCATGGTG
  AAGCACACAGCCACCCACCTGCTGAACTGGGCACTTCGGCAGACGCTCGGACCAACCACCGAACAGCGGG
  GCTCCCATCTCAACCCGGAGCGGCTGCGCTTTGATGTGGCCACGCAGACCCCACTGACCACAGAGCAGCT
  ACGGACAGTAGAGAGCTACGTGCAGGAAGCTGTGGGGCAGGATAAGCCTGTATACATGGAGGAAGTGCCC
  CTGGCACACACTGCCCGTATCCCCGGCCTTCGCTCACTGGATGAGGTGTACCCAGACCCCGTTCGGGTCG
  TGTCAGTGGGGGTTCCTGTGGCCCAGGCACTGGCACCAGCCTCCCAAGCTGCACTGCAGACTTCGGTGGA
  GCTGTGCTGCGGGACGCACCTGCTAAGTACTGGGGCTGTGGGAGACCTGGTGATTATTGGGGATCGCCAG
  CTGGTCAAAGGCATCACTCGCCTACTGGCCATCACCGGGGAGCAGGCCCAACAGGCCCGAGAGGTGGGCC
  AGAGCCTGTCGCAGGAGGTGGAAGTGGCAAGCGAACGGCTAAGTCGGGGCAGCCGGGACCTGCTGGAGGC
  TCACCGGCTATCTAAGGACATAGGACGTCTCATTGAGTTCACAGAGTCTGCTGTGATACCTCAGTGGCAG
  CGGCAGGAGCAGCAGACCACACTGAAGATGCTACAGCGCCGTGCCAACACCGCCATCCGGAAACTGGAGA
  AGAGCCAGGCTACGGAGAAATCCCAGGAGCTGTTGAAACGGCACTCGGAGGGGCCTCTGATTGTGGACAC
  TGTCTCCGCCCAGTCCCTCTCGGTGCTGGTAAAGGTCGTGCGGCAGCTGTGCAAACAGGCCCCCAGCATG
  TCCGTGCTGCTGCTCAGCCCCCAGCCCACTGGCAGTGTCCTGTGTGCCTGCCAGGTGGCCCAGGGTGCCA
  CACCTACCTTCACAGCTGAGGCCTGGGCCCTAGCTGTGTGCAGCCACATGGGAGGCAAGGCTTGGGGCTC
  TCCAGTGATAGCCCAGGGTACTGGACACACTGCTGACCTGGAGGCTGCCCTCAGGACAGCCCGAGCTTAT
  GCACTCAACCAGCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR204715 representing NM_001106891
  Red=Cloning site Green=Tags(s)
  
  MAASVAAAAGRLRRAIGRSCPWQRFSTEPHPPHGAAVRDAFLSFFRDRHGHRLVPSASVRPRGDPSLLFV
  NAGMNQFKPIFLGTVDPRSEMAGFRRVANSQKCVRAGGRHNDLEDVGRDLSHHTFFEMLGNWAFGGEYFK
  KEACSMAWELLTQVYGIPEDRLWVSYFSGDSKTGLDPDLETRDIWLSLGVPASRVLSFGLQENFWEMGDT
  GPCGPCTEIHYDLAGGMGPPQLVELWNLVFMQHYREADGSLHLLPQQHVDTGMGLERLVAVLQGKHSTYD
  TDLFSPLLDAIHQSCRVPPYSGRVGAADEGRIDTAYRVVADHIRTLSVCIADGVSPGMSGAPLVLRRILR
  RAVRYSTEVLQAPPGFLGNLVPVVVATLGAAYPELQKNSVKVLIWEIANLVSEDEAAFLASLQRGRRIID
  RTVKRLGPSDLFPAEVAWSLSLSGNLGIPLDLVQLMLEEKGVKLDTAGLEQLAQKEAQHRAQQAEAAQEE
  GLCLDVHALEELHRQGIPTTDDSPKYNYSLRPNGDYEFGLCEAQVLQLYSETGTAVASVGEGQRCGLLLD
  RTNFYAEQGGQASDRGYLIRTGQQDVLFPVARAQVCGGFILHEAMAPECLQVGDRVQLYVDKAWRMGCMV
  KHTATHLLNWALRQTLGPTTEQRGSHLNPERLRFDVATQTPLTTEQLRTVESYVQEAVGQDKPVYMEEVP
  LAHTARIPGLRSLDEVYPDPVRVVSVGVPVAQALAPASQAALQTSVELCCGTHLLSTGAVGDLVIIGDRQ
  LVKGITRLLAITGEQAQQAREVGQSLSQEVEVASERLSRGSRDLLEAHRLSKDIGRLIEFTESAVIPQWQ
  RQEQQTTLKMLQRRANTAIRKLEKSQATEKSQELLKRHSEGPLIVDTVSAQSLSVLVKVVRQLCKQAPSM
  SVLLLSPQPTGSVLCACQVAQGATPTFTAEAWALAVCSHMGGKAWGSPVIAQGTGHTADLEAALRTARAY
  ALNQL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001106891
• ORF Size: 2955 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001106891.1, NP_001100361.1
• RefSeq Size: 3323 bp
• RefSeq ORF: 2958 bp
• Locus ID: 301254
• UniProt ID: D3ZX08
• Cytogenetics: 9q12
• MW: 107.8 kDa
• Gene Summary: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain.[UniProtKB/Swiss-Prot Function]