Tlr3 (NM_198791) Rat Tagged ORF Clone

CAT#: RR204465

Tlr3 (Myc-DDK-tagged ORF) - Rat toll-like receptor 3 (Tlr3), (10 ug)

Product Images

Circular map for RR204465

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tlr3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR204465 representing NM_198791
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAGGGCGTTCATCTTATCTAATATACTCCTTTGGGGGACTTTTGTCCCTATGGATTCTTGTGGTGT
  CTTCCACAAACCAATGCACTGTGAGATACAACGTAGCTGACTGCAGCCATTTGAAGCTGACACACATACC
  TGATGACCTTCCCTCTAATATAACAGTGTTGAATCTTACTCACAATCAACTCAGAGGATTACCACCTGCC
  AATTTTACGAGATACAGCCAACTTGCTCTCTTGGATGCAGGATTCAACTCCATTTCAAAACTGGAGCCAG
  AACTGTGCCAAATTCTCCCTTTGTTGAAGGTATTGAACCTGCAACACAATGAGCTGTCTCAGATTTCTGA
  TCAAACCTTCGCCTTCTGCACGAACCTGACAGAGCTCCATCTAATGTCCAACTCAATACGCAAAATTAAA
  AGCAACCCTTTCAAAAACCAGAAGAGTCTAATCAAACTAGATTTGTCTCATAATGGCTTATCATCTACAA
  AGTTGGGAACTTGGGTCCAACTGGAGAACCTCCAAGAGCTGCTCTTAGCTAAAAATAAAATTTTTGCACT
  ACGAAATGAAGAACTTGATTTTCTTGGCAATTCTTCCTTACAAAAATTGGACTTGTCATCAAATCCACTT
  AAAGAGTTTTCTCCAGGGTGTTTCCATGCGATTGGCAAGTTATTCGTCCTCCTCTTGAACAATGCCCAAC
  TGAACCTCGACCTCACAGAGAAGCTTTGTTGGGAACTTTCAAACACAAGCATCCAGAATCTCTCTCTGGC
  TAACAACCAGCTGTTAGCAACCAGCAACAGCACTTTCTCCGGGCTGAAGCAGACCAACCTCACCTCGCTT
  GATCTTTCCTACAACAGCCTCCGCGACGTCGGTAACGATGCCTTCTCTTGGCTCCCGCACCTGAAGTATC
  TGTCCCTCGAGTACAATAATATACAGCGTTTGACCCCTCACTCTTTTCGTGGACTTTCCAACCTGAGGTA
  CCTGAGTTTGAAGCGAGCATTTACTAAGCAAAGTGTTGCACTTGCTTCTCACCCCAACATTGACGATTTT
  TCCTTTCAGTGGCTAAAATGTTTGGAACATCTCAACATGGACGATAATACTATACCGGGTATAAAAAGCA
  ATACTTTCACAGGATTGGTGAGTCTGAAGTACCTAAGTCTTTCCAAAACTTTCACGGGTTTGCAAACGTT
  AACAAACGAAACGTTTGTGTCTCTTACTCATTCTCCTTTGCTCACTCTCAATTTAACGAAGAATCACATC
  TCGAAGATAGCAAGTGGTACTTTCTCTTGGTTGGGTCAACTCAGGATACTTGATCTTGGCCTTAATGAAA
  TTGAGCAAGAACTCACCGGCCAGGAATGGAGAGGTCTGGGAAATATATTTGAGATCTACCTGTCCTATAA
  TAAATACCTCCAACTGACTAGCAAGTCCTTCACTTTGGTTCCCAGCCTTCAACGACTGATGCTCAGGAGG
  GTGGCCCTTAAAAGTGTGGATATCTCCCCTTCGCCTTTCCGTCCCCTTTATAACCTGACTATTCTGGACT
  TAAGCAACAACAACATTGCCAACCTGAACGAGGACTTGTTGGAGGGTCTTGAGAATCTAGAAATTCTGGA
  TTTTCAGCACAACAACTTAGCAAGACTCTGGAAACACGCAAACCCAGGTGGTCCCGTTAATTTCCTGAAG
  GGTCTGTCTCACCTCCACATCTTGAACTTAGAATCCAATGGCTTAGATGAAATCCCGGTCAAGGTGTTCA
  AGAACTTATTCGAACTCAAGAGCATCAATCTAGGACTGAATAATTTAAACACGCTTCTACCATCCATTTT
  TGATGACCAGACATCTCTAAGGTCACTGAACCTTCAGAAGAACCTCATCACATCAGTTGAGAAGAGTGTT
  TTCGGGCCAGCTTTTCACAACCTGAACAGCTTAGATATGAGCTTCAATCCGTTCGACTGTACATGTGAAA
  GCATTGCCTGGTTTGTTACCTGGCTTAACCAGACCCACACTAATATCCCTGAACTATCCACTCATTACCT
  CTGCAATACCCCCCAGCGTTACCACGGCCTCCCAGTGAAGCTTTTTGACACATCATCCTGTAAAGACAGT
  GCCCCCTTCCAACTCCTATTCATAATTAACACCAGTACGCTCCTGACTTTTATACTTGCGGTACTGCTCA
  TTCACTTCGAGGGTTGGAGGATATCTTTTTACTGGAATGTTTCAGTGCATCGAATTCTTGGTTTCAAGGA
  AATAGATGCACAGGGTGAGCAGTTCGAATACACGGCATACATAATTCATGCCCAGAAAGACAGAGACTGG
  GTCTGGGAGCATTTCTCCCCAATGGAAGAACAAGACCAATCTCTCAAATTTTGCCTAGAAGAAAGGGACT
  TCGAAGCAGGGGTCCTTGGACTTGAAGCAATTGTTAACAGCATCAAAAGAAGCAGAAAAATCATCTTTGT
  TATAACGCATCACCTACTGAAAGACCCGCTGTGCAGAAGATTCAAGGTGCATCACGCGGTTCAGCAAGCT
  ATCGAGCAAAATCTGGATTCAATTATACTGATTTTTCTCCAGAATATTCCAGATTACAAACTAAACCATG
  CACTCTGCTTGCGAAGAGGAATGTTTAAATCTCATTGCATCTTGAACTGGCCAATTCAAAAGGAACGGAT
  AAACGCCTTTCATCATAAACTGCAAGTAGCACTTGGGTCTCGGAATTCAGCACAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR204465 representing NM_198791
  Red=Cloning site Green=Tags(s)
  
  MKGRSSYLIYSFGGLLSLWILVVSSTNQCTVRYNVADCSHLKLTHIPDDLPSNITVLNLTHNQLRGLPPA
  NFTRYSQLALLDAGFNSISKLEPELCQILPLLKVLNLQHNELSQISDQTFAFCTNLTELHLMSNSIRKIK
  SNPFKNQKSLIKLDLSHNGLSSTKLGTWVQLENLQELLLAKNKIFALRNEELDFLGNSSLQKLDLSSNPL
  KEFSPGCFHAIGKLFVLLLNNAQLNLDLTEKLCWELSNTSIQNLSLANNQLLATSNSTFSGLKQTNLTSL
  DLSYNSLRDVGNDAFSWLPHLKYLSLEYNNIQRLTPHSFRGLSNLRYLSLKRAFTKQSVALASHPNIDDF
  SFQWLKCLEHLNMDDNTIPGIKSNTFTGLVSLKYLSLSKTFTGLQTLTNETFVSLTHSPLLTLNLTKNHI
  SKIASGTFSWLGQLRILDLGLNEIEQELTGQEWRGLGNIFEIYLSYNKYLQLTSKSFTLVPSLQRLMLRR
  VALKSVDISPSPFRPLYNLTILDLSNNNIANLNEDLLEGLENLEILDFQHNNLARLWKHANPGGPVNFLK
  GLSHLHILNLESNGLDEIPVKVFKNLFELKSINLGLNNLNTLLPSIFDDQTSLRSLNLQKNLITSVEKSV
  FGPAFHNLNSLDMSFNPFDCTCESIAWFVTWLNQTHTNIPELSTHYLCNTPQRYHGLPVKLFDTSSCKDS
  APFQLLFIINTSTLLTFILAVLLIHFEGWRISFYWNVSVHRILGFKEIDAQGEQFEYTAYIIHAQKDRDW
  VWEHFSPMEEQDQSLKFCLEERDFEAGVLGLEAIVNSIKRSRKIIFVITHHLLKDPLCRRFKVHHAVQQA
  IEQNLDSIILIFLQNIPDYKLNHALCLRRGMFKSHCILNWPIQKERINAFHHKLQVALGSRNSAH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198791
• ORF Size: 2715 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198791.1, NP_942086.1
• RefSeq Size: 3398 bp
• RefSeq ORF: 2718 bp
• Locus ID: 364594
• Cytogenetics: 16q11
• MW: 103.2 kDa
• Gene Summary: human homolog induces activation of NF-kappaB; mouse homolog plays a role in the production of inflammatory cytokines [RGD, Feb 2006]