Coro6 (NM_139115) Rat Tagged ORF Clone

CAT#: RR204362

Coro6 (Myc-DDK-tagged ORF) - Rat coronin, actin binding protein 6 (Coro6), (10 ug)

Product Images

Circular map for RR204362

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Coro6
• Synonyms: MGC112600
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR204362 representing NM_139115
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCCGTCGTGTGGTTCGGCAAAGCAAGTTCCGCCATGTGTTTGGTCAGGCAGCAAAGGCCGACCAGG
  CCTATGAGGACATCCGGGTATCCAAGGTCACATGGGACAGCGCCTTCTGTGCCGTCAACCCCAAATTTCT
  AGCCATTATTGTGGAGGCTGGAGGTGGAGGCGCCTTCATTGTCCTGCCTCTGGCCAAGACAGGACGAGTG
  GATAAGAACTACCCACTGGTCACTGGACACACTGGCCCTGTGCTGGACATTGACTGGTGCCCACACAATG
  ACAACGTCATCGCCAGTGCCTCAGATGACACCACTGTCATGGTGTGGCAGATTCCAGACTACACCCCAGT
  GCGAAACATCACAGAACCTGTCATCACACTCGAGGGCCACTCCAAGCGTGTGGGCATCCTGTCTTGGCAT
  CCTACTGCCAGGAATGTCCTGCTCAGTGCAGGTGGTGATAATGTGATCATCATCTGGAATGTGGGCACTG
  GGGAGGTACTGCTGAGCCTAGACGACATACACCCTGACGTCATCCACAGCGTGTGTTGGAACAGCAATGG
  TAGCCTGCTAGCCACCACTTGCAAGGACAAGACCTTGCGTATCATTGACCCCCGGAAGAGTCAAGTGGTT
  GCGGAGAGGTTTGCAGCCCACGAGGGGATGAGGCCCATGCGGGCTGTCTTCACACGGCTGGGTCATATCT
  TCACCACGGGATTCACCCGCATGAGCCAGCGAGAGCTAGGCCTGTGGGACCCGAACAACTTCGAAGAACC
  AGTGGCCCTGCAGGAAATGGACACAAGCAACGGAGTGTTACTACCATTCTACGATCCTGACTCCAGCATC
  GTCTACTTGTGTGGCAAGGGTGACAGTAGCATTCGGTACTTTGAAATTACGGACGAACCACCTTTCGTGC
  ATTATCTGAACACATTTAGCAGCAAAGAACCGCAACGGGGCATGGGTTTCATGCCCAAGCGGGGACTGGA
  TGTCAGCAAATGTGAAATCGCCAGGTTCTACAAGCTACACGAACGGAAGTGTGAACCCATCGTCATGACT
  GTACCTCGAAAGTCAGATCTGTTCCAGGATGACCTATACCCGGACACGCCTGGCCCCGAGCCAGCCCTGG
  AAGCTGATGAGTGGCTATCGGGTCAGGATGCAGAACCTGTGCTCATTTCGCTGAAGGAAGGCTACGTTCC
  CCCCAAACACCGTGAGTTCCGGGTCACCAAACGCAACATCCTAGATGTGCGCCCGCCGGCCAGTCCTCGG
  CGCAGCCAGTCAGCCAGCGAAGCCCCCTTGTCGCAGCAGCACACCCTAGAGACACTGCTGGAGGAGATGA
  AGGCCCTCCGAGAGCGGGTGCAAGCCCAAGAGGAGCGCATTACCGCGTTGGAAAACATGCTGTGTGAGCT
  GGTGGATGGCACGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR204362 representing NM_139115
  Red=Cloning site Green=Tags(s)
  
  MSRRVVRQSKFRHVFGQAAKADQAYEDIRVSKVTWDSAFCAVNPKFLAIIVEAGGGGAFIVLPLAKTGRV
  DKNYPLVTGHTGPVLDIDWCPHNDNVIASASDDTTVMVWQIPDYTPVRNITEPVITLEGHSKRVGILSWH
  PTARNVLLSAGGDNVIIIWNVGTGEVLLSLDDIHPDVIHSVCWNSNGSLLATTCKDKTLRIIDPRKSQVV
  AERFAAHEGMRPMRAVFTRLGHIFTTGFTRMSQRELGLWDPNNFEEPVALQEMDTSNGVLLPFYDPDSSI
  VYLCGKGDSSIRYFEITDEPPFVHYLNTFSSKEPQRGMGFMPKRGLDVSKCEIARFYKLHERKCEPIVMT
  VPRKSDLFQDDLYPDTPGPEPALEADEWLSGQDAEPVLISLKEGYVPPKHREFRVTKRNILDVRPPASPR
  RSQSASEAPLSQQHTLETLLEEMKALRERVQAQEERITALENMLCELVDGTD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_139115
• ORF Size: 1416 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_139115.2, NP_620815.2
• RefSeq Size: 2444 bp
• RefSeq ORF: 1419 bp
• Locus ID: 245982
• UniProt ID: Q920J3
• Cytogenetics: 10q24
• MW: 52.9 kDa
• Gene Summary: contains a WD40 domain [RGD, Feb 2006]