Pus1 (NM_001025563) Rat Tagged ORF Clone
CAT#: RR204099
- TrueORF®
Pus1 (Myc-DDK-tagged ORF) - Rat pseudouridylate synthase 1 (Pus1), (10 ug)
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_001025563" in other vectors (3)
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Pus1 |
Synonyms | MGC112655 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR204099 representing NM_001025563
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCTTCCCCCGCCTGTGGGCCGCACTGCTGAGAGCCTGGGGACGGTGGACGGCGCGCCCAGGCCCGC GCGTGCCCGGCCTGCCGCCTATGGCTGGAAACAAGGTGCCACCAGCGCTGCCCTCACACCAGCCGGACAG GAAGGGCCGAGGTGGCTGGGTTTGGGAGGAGACGGAGCATCCTGCCAAGAGGGTCAAGGGCGGTGAGGAT GAAGAGCCTCCACGCAAGCTGCCAAAGCGCAAAATTGTGCTGCTCATGGCCTACTCCGGCAAGGGCTACC ACGGCATGCAGAGGAATCTGGGGTCCTCCCAGTTCAGGACCATCGAAGATGACTTGGTATCTGCCTTAGT CCAGGCAGGTTGTATCCCTGAAAACCATGGCACAGATATGAGGAAGATGTCTTTCCAGCGATGTGCTCGC ACAGACAAGGGTGTGTCTGCAGCTGGGCAGGTGGTATCCCTAAAGGTGTGGCTAATTGATGACATCCTGG ACAAGATCAACAGCCACCTTCCATCCCATATTCGGATTCTGGGACTGAAGAGGGTCACAGGTGGGTTCAA CTCCAAGAACAAGTGTGACGCCAGGACCTATTGCTACATGCTACCTACCTTTGCCTTTGCTCATAAGGAC CGGGATGTACAGGATGAGAGCTATCGCCTAAGTGCAGAGACCCTGCAGCAGGTCAACCGGCTTCTGGGCT GCTACAAAGGCACGCATAACTTCCATAACTTCACATCACAGAAGGGGCCGAGAGAGCCTAGTGCACGCCG CTACATTCTGGAGATGTACTGCGAGGAGCCCTTTGTTCGGGAGGGCCTGGAGTTTGCTGTGATCAAGGTG AAGGGCCAGAGCTTCATGATGCACCAGATCCGGAAGATGGTTGGCCTGGTGGTAGCCATTGTCAAGGGTT ATGCCCCTGAGAGTGTCCTGGAGCGAAGTTGGGGTGAGGAGAAGGTGGATGTGCCCAAGGCACCAGGGCT TGGCTTAGTCCTGGAGAGGGTTCACTTTGAGAAGTACAATCAACGCTTTGGCAGTGACGGGCTGCATGAG CCCCTGGACTGGGCACAGGAGGAAGGGAAGGTGACTGCTTTCAAGGAGCAATACATCTACCCTACCATTG TCAGCACTGAGCGGGATGAGCGGTCCATGGCCCAGTGGCTTAACACTTTGCCGATCCACAACTTCAGTGG CACTGCACTTGGAGCGGATGACACAGGTGCCAAGGTCCCCAGCTCCCTGGAGGGCAGTGAAGGGGATGGA GACACTGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR204099 representing NM_001025563
Red=Cloning site Green=Tags(s) MGFPRLWAALLRAWGRWTARPGPRVPGLPPMAGNKVPPALPSHQPDRKGRGGWVWEETEHPAKRVKGGED EEPPRKLPKRKIVLLMAYSGKGYHGMQRNLGSSQFRTIEDDLVSALVQAGCIPENHGTDMRKMSFQRCAR TDKGVSAAGQVVSLKVWLIDDILDKINSHLPSHIRILGLKRVTGGFNSKNKCDARTYCYMLPTFAFAHKD RDVQDESYRLSAETLQQVNRLLGCYKGTHNFHNFTSQKGPREPSARRYILEMYCEEPFVREGLEFAVIKV KGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGEEKVDVPKAPGLGLVLERVHFEKYNQRFGSDGLHE PLDWAQEEGKVTAFKEQYIYPTIVSTERDERSMAQWLNTLPIHNFSGTALGADDTGAKVPSSLEGSEGDG DTD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001025563 |
ORF Size | 1269 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001025563.1, NP_001020734.1 |
RefSeq Size | 1809 bp |
RefSeq ORF | 1272 bp |
Locus ID | 304567 |
UniProt ID | Q4KM92 |
Cytogenetics | 12q16 |
MW | 47.5 kDa |
Gene Summary | Converts specific uridines to PSI in a number of tRNA substrates. Acts on positions 27/28 in the anticodon stem and also positions 34 and 36 in the anticodon of an intron containing tRNA. Involved in regulation of nuclear receptor activity through pseudouridylation of SRA1 RNA.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN204099 | Pus1 (untagged ORF) - Rat pseudouridylate synthase 1 (Pus1), (10 ug) |
USD 503.00 |
|
RR204099L3 | Lenti ORF clone of Pus1 (Myc-DDK-tagged ORF) - Rat pseudouridylate synthase 1 (Pus1), (10 ug) |
USD 803.00 |
|
RR204099L4 | Lenti ORF clone of Pus1 (mGFP-tagged ORF) - Rat pseudouridylate synthase 1 (Pus1), (10 ug) |
USD 803.00 |
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