Pus1 (NM_001025563) Rat Tagged ORF Clone

CAT#: RR204099

Pus1 (Myc-DDK-tagged ORF) - Rat pseudouridylate synthase 1 (Pus1), (10 ug)

Product Images

Circular map for RR204099

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pus1
• Synonyms: MGC112655
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR204099 representing NM_001025563
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTTCCCCCGCCTGTGGGCCGCACTGCTGAGAGCCTGGGGACGGTGGACGGCGCGCCCAGGCCCGC
  GCGTGCCCGGCCTGCCGCCTATGGCTGGAAACAAGGTGCCACCAGCGCTGCCCTCACACCAGCCGGACAG
  GAAGGGCCGAGGTGGCTGGGTTTGGGAGGAGACGGAGCATCCTGCCAAGAGGGTCAAGGGCGGTGAGGAT
  GAAGAGCCTCCACGCAAGCTGCCAAAGCGCAAAATTGTGCTGCTCATGGCCTACTCCGGCAAGGGCTACC
  ACGGCATGCAGAGGAATCTGGGGTCCTCCCAGTTCAGGACCATCGAAGATGACTTGGTATCTGCCTTAGT
  CCAGGCAGGTTGTATCCCTGAAAACCATGGCACAGATATGAGGAAGATGTCTTTCCAGCGATGTGCTCGC
  ACAGACAAGGGTGTGTCTGCAGCTGGGCAGGTGGTATCCCTAAAGGTGTGGCTAATTGATGACATCCTGG
  ACAAGATCAACAGCCACCTTCCATCCCATATTCGGATTCTGGGACTGAAGAGGGTCACAGGTGGGTTCAA
  CTCCAAGAACAAGTGTGACGCCAGGACCTATTGCTACATGCTACCTACCTTTGCCTTTGCTCATAAGGAC
  CGGGATGTACAGGATGAGAGCTATCGCCTAAGTGCAGAGACCCTGCAGCAGGTCAACCGGCTTCTGGGCT
  GCTACAAAGGCACGCATAACTTCCATAACTTCACATCACAGAAGGGGCCGAGAGAGCCTAGTGCACGCCG
  CTACATTCTGGAGATGTACTGCGAGGAGCCCTTTGTTCGGGAGGGCCTGGAGTTTGCTGTGATCAAGGTG
  AAGGGCCAGAGCTTCATGATGCACCAGATCCGGAAGATGGTTGGCCTGGTGGTAGCCATTGTCAAGGGTT
  ATGCCCCTGAGAGTGTCCTGGAGCGAAGTTGGGGTGAGGAGAAGGTGGATGTGCCCAAGGCACCAGGGCT
  TGGCTTAGTCCTGGAGAGGGTTCACTTTGAGAAGTACAATCAACGCTTTGGCAGTGACGGGCTGCATGAG
  CCCCTGGACTGGGCACAGGAGGAAGGGAAGGTGACTGCTTTCAAGGAGCAATACATCTACCCTACCATTG
  TCAGCACTGAGCGGGATGAGCGGTCCATGGCCCAGTGGCTTAACACTTTGCCGATCCACAACTTCAGTGG
  CACTGCACTTGGAGCGGATGACACAGGTGCCAAGGTCCCCAGCTCCCTGGAGGGCAGTGAAGGGGATGGA
  GACACTGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR204099 representing NM_001025563
  Red=Cloning site Green=Tags(s)
  
  MGFPRLWAALLRAWGRWTARPGPRVPGLPPMAGNKVPPALPSHQPDRKGRGGWVWEETEHPAKRVKGGED
  EEPPRKLPKRKIVLLMAYSGKGYHGMQRNLGSSQFRTIEDDLVSALVQAGCIPENHGTDMRKMSFQRCAR
  TDKGVSAAGQVVSLKVWLIDDILDKINSHLPSHIRILGLKRVTGGFNSKNKCDARTYCYMLPTFAFAHKD
  RDVQDESYRLSAETLQQVNRLLGCYKGTHNFHNFTSQKGPREPSARRYILEMYCEEPFVREGLEFAVIKV
  KGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGEEKVDVPKAPGLGLVLERVHFEKYNQRFGSDGLHE
  PLDWAQEEGKVTAFKEQYIYPTIVSTERDERSMAQWLNTLPIHNFSGTALGADDTGAKVPSSLEGSEGDG
  DTD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001025563
• ORF Size: 1269 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025563.1, NP_001020734.1
• RefSeq Size: 1809 bp
• RefSeq ORF: 1272 bp
• Locus ID: 304567
• UniProt ID: Q4KM92
• Cytogenetics: 12q16
• MW: 47.5 kDa
• Gene Summary: Converts specific uridines to PSI in a number of tRNA substrates. Acts on positions 27/28 in the anticodon stem and also positions 34 and 36 in the anticodon of an intron containing tRNA. Involved in regulation of nuclear receptor activity through pseudouridylation of SRA1 RNA.[UniProtKB/Swiss-Prot Function]