Htr4 (NM_012853) Rat Tagged ORF Clone

CAT#: RR203539

Htr4 (Myc-DDK-tagged ORF) - Rat 5-hydroxytryptamine (serotonin) receptor 4 (Htr4), (10 ug)

Product Images

Circular map for RR203539

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Htr4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR203539 representing NM_012853
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACAGACTTGATGCTAATGTGAGTTCCAACGAGGGTTTCGGGTCTGTGGAGAAGGTCGTACTGCTCA
  CGTTCTTCGCAATGGTTATCCTGATGGCCATCCTGGGCAACCTGCTGGTGATGGTTGCTGTGTGCAGGGA
  CAGGCAGCTCAGGAAAATAAAAACCAATTATTTCATTGTGTCTCTTGCCTTTGCTGATCTGCTGGTTTCG
  GTGCTGGTGAATGCCTTCGGTGCCATTGAGTTGGTCCAAGACATCTGGTTTTATGGGGAGATGTTTTGCC
  TGGTCCGGACCTCTCTGGATGTCCTACTCACCACAGCATCAATTTTTCACCTCTGCTGCATTTCCCTGGA
  TAGGTATTATGCCATCTGCTGTCAACCTTTGGTTTATAGAAACAAGATGACCCCTCTACGCATCGCATTA
  ATGCTGGGAGGCTGCTGGGTCATTCCCATGTTTATATCTTTTCTCCCCATAATGCAAGGCTGGAACAACA
  TCGGCATAGTTGATGTGATAGAGAAAAGGAAATTCAACCACAACTCTAACTCTACATTCTGTGTCTTCAT
  GGTCAACAAGCCCTATGCCATCACCTGCTCTGTGGTGGCCTTCTACATCCCGTTTCTCCTCATGGTGCTG
  GCCTATTACCGTATCTATGTCACTGCTAAGGAGCATGCCCAGCAGATCCAGATGTTACAACGGGCAGGAG
  CCACCTCTGAAAGCAGGCCCCAGACAGCTGACCAGCACAGCACACATCGCATGCGGACAGAGACCAAAGC
  AGCCAAGACTTTATGTGTCATCATGGGCTGCTTCTGTTTCTGCTGGGCCCCCTTCTTTGTCACCAATATT
  GTGGACCCTTTCATAGACTACACTGTGCCCGAGAAGGTGTGGACTGCTTTCCTCTGGCTTGGCTATATCA
  ATTCAGGGTTGAACCCTTTTCTCTATGCCTTCTTGAATAAGTCTTTCAGACGTGCCTTCCTTATCATCCT
  CTGCTGTGATGATGAGCGCTACAAAAGACCCCCCATTCTGGGCCAGACTGTCCCCTGTTCAACCACAACC
  ATTAATGGATCCACTCATGTGCTAAGGGATACAGTGGAATGTGGTGGCCAATGGGAGAGTCGGTGTCACC
  TCACAGCAACTTCTCCTTTGGTGGCTGCTCAGCCAGTGATACGTAGGCCCCAGGACAATGACCTAGAAGA
  CAGCTGTAGCTTGAAAAGAAGCCAGTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR203539 representing NM_012853
  Red=Cloning site Green=Tags(s)
  
  MDRLDANVSSNEGFGSVEKVVLLTFFAMVILMAILGNLLVMVAVCRDRQLRKIKTNYFIVSLAFADLLVS
  VLVNAFGAIELVQDIWFYGEMFCLVRTSLDVLLTTASIFHLCCISLDRYYAICCQPLVYRNKMTPLRIAL
  MLGGCWVIPMFISFLPIMQGWNNIGIVDVIEKRKFNHNSNSTFCVFMVNKPYAITCSVVAFYIPFLLMVL
  AYYRIYVTAKEHAQQIQMLQRAGATSESRPQTADQHSTHRMRTETKAAKTLCVIMGCFCFCWAPFFVTNI
  VDPFIDYTVPEKVWTAFLWLGYINSGLNPFLYAFLNKSFRRAFLIILCCDDERYKRPPILGQTVPCSTTT
  INGSTHVLRDTVECGGQWESRCHLTATSPLVAAQPVIRRPQDNDLEDSCSLKRSQS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012853
• ORF Size: 1218 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012853.1, NP_036985.1
• RefSeq Size: 1260 bp
• RefSeq ORF: 1221 bp
• Locus ID: 25324
• UniProt ID: Q62758
• Cytogenetics: 18q12.1
• MW: 46.1 kDa
• Gene Summary: receptor for serotonin; may play a role in cognition and anxiolysis [RGD, Feb 2006]