Ralgds (NM_019250) Rat Tagged ORF Clone

CAT#: RR203249

Ralgds (Myc-DDK-tagged ORF) - Rat ral guanine nucleotide dissociation stimulator (Ralgds), (10 ug)

Product Images

Circular map for RR203249

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ralgds
• Synonyms: Rgds
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR203249 representing NM_019250
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCAGCGCATGTGGGCCGAGGCGTCCGGGCCCATAGGGGGCGCCGAGCCGTTGTTTCCCGGCTCCC
  GGCGCAGCCGCAGCGTGTGGGACGCCGTGCGTCTGGAGGTGGGCGTCCCTGACAGCTGTCCAGTGGTGCT
  GCACAGCTTCACGCAGCTCGACCCCGATCTGCCGCGCCTGGAGAGCTCTACGCAGGAGATTGGGGAGGAG
  CTGATCAACGGGGTCATCTACTCCATCTCCCTGCGCAAGGTTCAGCTACACCCAGGAGCCACCAAGGGCC
  AGCGCTGGCTAGGGTGCGAGAATGAGTCAGCTCTGAACCTCTACGAGACTTGCAAGGTGCGCACGGTGAA
  GGCCGGTACTCTGGAGAAGCTGGTGGAACACCTGGTGCCTGCCTTCCAGGGCAGTGACCTTTCCTACGTC
  ACCGTATTCCTGTGCACCTACAGAGCCTTCACAACCACTCAGCAGGTGCTAGACCTGCTGTTCAAAAGAT
  ATGGCTGCATCCTTCCCTACTCCAGTGAGGACGGCGGACCACAGGACCAACTCAAAAATGCCATCTCTTC
  CATCCTGGGCACCTGGCTGGACCAATACTCCGAGGACTTCTGTCAACCCCCGGACTTTCCCTGCCTCAAG
  CAGCTGGTGGCTTATGTGCAGCTCAACATGCCTGGCTCAGACCTGGAGCGCCGTGCTCACCTTCTCCTGG
  CCCAGCTGGAGGACCTGGAGCCCAGTGAGGTTGAGCCTGAGGCCCTGTCCCCAGCTCCAGTGCTGTCTCT
  GAAACCAGCTTCACAGCTAGAACCAGCTCCCGCCCTGCTTCTGACGCCCAGCCGAGCTGTGGCATCCACT
  CCAGTCCGAGAGCCAGCCCCGGTGCCAGTACTGGCATCCAGCCCGGTGGTGGCGCCAGCCTCTGAGCTAG
  AACCAGCTCTGGAGCCGCCCCTAGACCCTGAGCCAACCCTCGCACCTGCTCCAGAGCTGGACCCCACTGT
  CTCACAGAGCCTCCACCTCGAGCCCGCTCCCGTGCCCGCTCCTGCATTAGAGCCTTCCTGGCCTCTGCCT
  GAAACCACGGAGAACGGACTGTGTGCAAAGCCTCACCTACTGCTGTTCCCTCCAGACTTGGTGGCTGAAC
  AGTTTACGCTGATGGACGCAGAGCTATTCAAGAAAGTTGTGCCCTACCACTGCCTGGGCTCCATCTGGTC
  CCAGCGGGACAAGAAGGGCAAGGAGCACCTCGCGCCCACCATCCGTGCCACTGTCGCCCAGTTCAACAAC
  GTGGCCAACTGTGTCATCACCACCTGCCTTGGGGACCAGAGTATGAAAGCTTCGGACAGGGCCCGGGTGG
  TGGAACACTGGATCGAGGTGGCCAGGGAGTGCAGGGTGCTCAAGAATTTCTCCTCCCTCTACGCCATCCT
  CTCTGCCCTACAGAGCAATGCCATCCACCGCCTAAAGAAGACATGGGAAGAGGTCTCCAGGGGCAGCTTT
  CGAGTGTTCCAGAAACTGTCAGAAATCTTCTCTGATGAGAACAACTACTCCTTGAGCAGAGAGCTGCTCA
  TCAAGGAAGGAACCTCCAAGTTTGCCACGCTGGAGATGAACCCTAGGAGAACCCAGAGGCGGCAGAAGGA
  GACAGGTGTCATCCAGGGCACTGTCCCCTACCTGGGCACATTCCTCACTGACCTGGTGATGCTGGACACA
  GCCATGAAGGACTATCTATATGGGAGACTGATCAACTTTGAAAAGAGGAGGAAGGAGTTCGAAGTCATCG
  CCCAGATCAAGTTGCTCCAGTCGGCCTGCAACAACTACAGCATTGTTCCCGAGGAGCACTTTGGAGCCTG
  GTTCCGAGCCATGGGACGGCTCAGCGAGGCTGAGAGCTACAACCTGTCGTGTGAGCTGGAGCCCCCGTCT
  GAGTCAGCCAGCAACACCCTGAGGAGCAAGAAAAGCACAGCTATCGTCAAGCGCTGGAGCGACCGCCAGG
  CTCCCAGCACGGAGCTCAGCACCAGTAGCAGTGCACACTCCAAGTCATGTGACCAGCTCCGGTGCAGCCC
  CTACCTCAGCAGCGGGGACATCACGGACGCACTCAGTGTGCACTCAGCTGGTTCATCCAGCTCTGACGTG
  GAGGAGATCAACATGAGCTTTGTTCCAGAGTCTCCTGATGGCCAGGAAAAGAAGTTCTGGGAGTCAGCCT
  CCCAGTCGTCCCCAGAGACCTCTGGCATCAGCTCGGCCTCCAGCAGCACCTCCTCTTCGTCAGCCTCTAC
  CACACCGGTGTCTACCACACGCACCCACAAGCGCTCTGTCTCAGGGGTCTGCAGCTACAGCTCCTCACTG
  CCACTCTACAACCAGCAGGTGGGCGACTGCTGCATCATCAGGGTCAGCCTGGATGTGGACAACGGCAACA
  TGTACAAGAGCATCCTGGTGACCAGCCAGGATAAGGCTCCGACTGTCATCCGGAAAGCCATGGACAAACA
  CAACCTAGATGAGGACGAGCCCGAGGACTATGAGCTGCTGCAGATCATCTCAGAGGACCACAAGCTGAAA
  ATTCCAGAGAACGCCAATGTGTTCTATGCCATGAACTCTGCCGCCAACTATGACTTCATCCTAAAGAAGC
  GGGCCTTCACTAAGGGGGCTAAAGTCAAGCATGGAGCCAGCTCCACTCTCCCTCGAATGAAGCAGAAGGG
  ACTCAGGATTGCCAGAGGCATCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR203249 representing NM_019250
  Red=Cloning site Green=Tags(s)
  
  MVQRMWAEASGPIGGAEPLFPGSRRSRSVWDAVRLEVGVPDSCPVVLHSFTQLDPDLPRLESSTQEIGEE
  LINGVIYSISLRKVQLHPGATKGQRWLGCENESALNLYETCKVRTVKAGTLEKLVEHLVPAFQGSDLSYV
  TVFLCTYRAFTTTQQVLDLLFKRYGCILPYSSEDGGPQDQLKNAISSILGTWLDQYSEDFCQPPDFPCLK
  QLVAYVQLNMPGSDLERRAHLLLAQLEDLEPSEVEPEALSPAPVLSLKPASQLEPAPALLLTPSRAVAST
  PVREPAPVPVLASSPVVAPASELEPALEPPLDPEPTLAPAPELDPTVSQSLHLEPAPVPAPALEPSWPLP
  ETTENGLCAKPHLLLFPPDLVAEQFTLMDAELFKKVVPYHCLGSIWSQRDKKGKEHLAPTIRATVAQFNN
  VANCVITTCLGDQSMKASDRARVVEHWIEVARECRVLKNFSSLYAILSALQSNAIHRLKKTWEEVSRGSF
  RVFQKLSEIFSDENNYSLSRELLIKEGTSKFATLEMNPRRTQRRQKETGVIQGTVPYLGTFLTDLVMLDT
  AMKDYLYGRLINFEKRRKEFEVIAQIKLLQSACNNYSIVPEEHFGAWFRAMGRLSEAESYNLSCELEPPS
  ESASNTLRSKKSTAIVKRWSDRQAPSTELSTSSSAHSKSCDQLRCSPYLSSGDITDALSVHSAGSSSSDV
  EEINMSFVPESPDGQEKKFWESASQSSPETSGISSASSSTSSSSASTTPVSTTRTHKRSVSGVCSYSSSL
  PLYNQQVGDCCIIRVSLDVDNGNMYKSILVTSQDKAPTVIRKAMDKHNLDEDEPEDYELLQIISEDHKLK
  IPENANVFYAMNSAANYDFILKKRAFTKGAKVKHGASSTLPRMKQKGLRIARGIF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019250
• ORF Size: 2685 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019250.2, NP_062123.2
• RefSeq Size: 3684 bp
• RefSeq ORF: 2688 bp
• Locus ID: 29622
• UniProt ID: Q03386
• Cytogenetics: 3p12
• MW: 98.8 kDa
• Gene Summary: facilitates conversion of Ras-related GTPase (Ral) to its GTP-bound form; may be involved in cardiac hypertrophy [RGD, Feb 2006]