Acadvl (NM_012891) Rat Tagged ORF Clone

CAT#: RR203012

Acadvl (Myc-DDK-tagged ORF) - Rat acyl-Coenzyme A dehydrogenase, very long chain (Acadvl), nuclear gene encoding mitochondrial protein, (10 ug)

Product Images

Circular map for RR203012

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Acadvl
• Synonyms: VLCAD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR203012 representing NM_012891
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGTCGGCCCGGATGACCCCGAGTGTGGGGCGACAACTGCTGCGGCTGGGGGCCCGAAGCTCGCGGT
  CTGCTGCGCTTCAGGGACAACCCCGGCCTACCTCTGCCCAGCGACTTTATGCCAGTGAGGCCACTCAGGC
  AGTTCTGGAAAAGCCAGAGACCCTCTCCTCTGATGCTTCCACCAGAGAAAAACCTGCCAGGGCGGAATCT
  AAGTCTTTTGCTGTGGGAATGTTCAAAGGCCAGCTTACCACCGACCAGGTGTTCCCATACCCATCTGTGC
  TCAATGAAGGACAGACACAATTTCTCAAAGAGCTGGTGGGACCAGTGGCCCGGTTCTTTGAAGAAGTGAA
  TGACCCTGCCAAGAATGACTCCTTGGAGAAGGTGGAGGAGGACACTTTGCAGGGACTCAAAGAACTGGGG
  GCATTTGGTCTGCAAGTACCCAGCGAGCTGGGTGGTTTGGGCCTCTCTAATACCCAGTACGCTCGCTTGG
  CAGAGATTGTGGGCATGCATGACCTTGGTGTTAGCGTTACCCTGGGAGCCCATCAGAGCATCGGTTTCAA
  AGGCATCTTGCTCTATGGCACAAAGGCCCAGAAGGAAAAATACCTCCCCAGAGTGGCATCCGGGCAGGCT
  TTGGCGGCTTTCTGCCTGACTGAGCCCTCGAGCGGGTCCGATGTGGCCTCTATCCGAAGCTCAGCTGTAC
  CTAGCCCCTGTGGAAAGTATTATACTCTCAACGGAAGCAAGATCTGGATCAGTAATGGGGGTCTGGCAGA
  CATTTTCACTGTCTTTGCCAAAACGCCAATTAAAGATGCAGCCACGGGGGCCGTGAAAGAGAAGATCACA
  GCTTTCGTAGTGGAACGGAGCTTTGGAGGGGTTACCCATGGGCTCCCCGAAAAGAAGATGGGCATCAAGG
  CATCTAACACATCAGAGGTGTACTTTGATGGAGTCAAGGTGCCAGCAGAGAATGTGCTAGGAGAAGTGGG
  AGATGGCTTCAAGGTTGCTGTCAACATCCTCAACAACGGAAGATTTGGGATGGCTGCAACCCTAGCAGGC
  ACCATGAAAGCCATCATTGCCAAGGCGGTTGATCATGCTACTAACCGTACCCAGTTTGGGGACAAAATTC
  ACAACTTTGGGGTGATCCAGGAAAAGCTGGCTCGGATGGCTATTCTGCAGTATGTGACTGAGTCCATGGC
  TTACATGCTGAGTGCCAACATGGACCAGGGATTCAAAGACTTCCAGATAGAAGCTGCCATCAGCAAAATC
  TTTGGCTCGGAGGCGGCCTGGAAAGTGACAGATGAGTGCATCCAGATAATGGGGGGCATGGGCTTCATGA
  AGGAACCAGGGGTAGAGCGTGTGCTCCGAGATATTCGAATCTTCCGGATCTTTGAAGGGACAAATGACAT
  TCTTCGACTGTTTGTGGCTCTACAAGGCTGCATGGACAAAGGAAAGGAACTCACGGGACTTGGTAATGCC
  CTAAAGAATCCTCTTGGAAATGTTGGCCTCCTCATAGGAGAAGCAAGCAAACAGCTGAGGCGGCGGACAG
  GGATTGGCAGTGGTCTGAGTCTCTCGGGAATTGTCCACCCAGAGTTGAGTCGCAGTGGTGAACTGGCAGT
  GCAGGCTCTGGAACAATTTGCCACTGTAGTGGAGGCGAAGCTGATGAAGCACAAGAAAGGGATTGTCAAT
  GAACAGTTCCTGCTGCAGCGACTGGCAGATGGAGCCATTGACCTCTACGCCATGGTGGTGGTTCTCTCCA
  GAGCCTCAAGATCCCTGAGTGAGGGCTACCCGACAGCACAGCATGAGAAAATGCTCTGTGATAGTTGGTG
  CATTGAGGCTGCAACACGGATTCGAGAAAACATGGCCAGCCTTCAGTCCAACCCTCAGCAGCAGGAGCTC
  TTTCGTAACTTCAGAAGTATCTCCAAGGCCATGGTGGAGAATGGTGGCCTGGTCACCAGTAACCCCCTTA
  GAGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR203012 representing NM_012891
  Red=Cloning site Green=Tags(s)
  
  MQSARMTPSVGRQLLRLGARSSRSAALQGQPRPTSAQRLYASEATQAVLEKPETLSSDASTREKPARAES
  KSFAVGMFKGQLTTDQVFPYPSVLNEGQTQFLKELVGPVARFFEEVNDPAKNDSLEKVEEDTLQGLKELG
  AFGLQVPSELGGLGLSNTQYARLAEIVGMHDLGVSVTLGAHQSIGFKGILLYGTKAQKEKYLPRVASGQA
  LAAFCLTEPSSGSDVASIRSSAVPSPCGKYYTLNGSKIWISNGGLADIFTVFAKTPIKDAATGAVKEKIT
  AFVVERSFGGVTHGLPEKKMGIKASNTSEVYFDGVKVPAENVLGEVGDGFKVAVNILNNGRFGMAATLAG
  TMKAIIAKAVDHATNRTQFGDKIHNFGVIQEKLARMAILQYVTESMAYMLSANMDQGFKDFQIEAAISKI
  FGSEAAWKVTDECIQIMGGMGFMKEPGVERVLRDIRIFRIFEGTNDILRLFVALQGCMDKGKELTGLGNA
  LKNPLGNVGLLIGEASKQLRRRTGIGSGLSLSGIVHPELSRSGELAVQALEQFATVVEAKLMKHKKGIVN
  EQFLLQRLADGAIDLYAMVVVLSRASRSLSEGYPTAQHEKMLCDSWCIEAATRIRENMASLQSNPQQQEL
  FRNFRSISKAMVENGGLVTSNPLRV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012891
• ORF Size: 1965 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012891.2, NP_037023.1
• RefSeq Size: 2115 bp
• RefSeq ORF: 1968 bp
• Locus ID: 25363
• UniProt ID: P45953
• Cytogenetics: 10q24
• MW: 70.7 kDa
• Gene Summary: catalyzes the beta-oxidation of long-chain fatty acids in mitochondrial fatty acid metabolism [RGD, Feb 2006]