Dgcr8 (NM_001105865) Rat Tagged ORF Clone

CAT#: RR202911

Dgcr8 (Myc-DDK-tagged ORF) - Rat DiGeorge syndrome critical region gene 8 (Dgcr8), (10 ug)

Product Images

Circular map for RR202911

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dgcr8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR202911 representing NM_001105865
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGACATATGAGAGTCCCTCTCCTCTCCCGCATGAGCCCACAGGAGAAGCAATGATGGAGAACCGAG
  CTTGCCCCTTCCAAGTGCTGCCCCATGAACAGTCTCCACCACCTCCCCTGCAAACGTCCAGTGATGCAGA
  GGTAATGGACGTTGGCTCTGGTGGTGATGGACAGTCCGAACCTCCTGCCGACGACCCATTCAACTTCTAC
  GGAGCTTCTCTTCTCTCCAAAGGATCCTTCTCTAAGGGCCGCCTCCTCATAGACCCGAACTGTAGTGGCC
  ACAGCCCGCGCACTGCCCGGCACGCACCTGCGGTCCGGAAGTTCTCCCCTGACCTTAAGTTGCTTAAGGA
  TGTAAAGATTAGCGTGAGCTTTACTGAGAGCTGCAGGAGTAAGGACAGGAAGGTGCTGTACACAGGAGTA
  GAACGCAGCACTCGGCCTGAATGTAGCCAGCTCCTTAGTCCTGTCTGCGGGGACGTGCATGCTTGTCCCT
  TTGGCGGGAGTGTTGGTAATGGGGTAGGCCTAGGGGGTGAGAGTACAGATAAGAAGGATGAGGAAAATGA
  GCTGGATCAGGAAAAGAGAGTGGAGTATGCAGTGCTCGATGAGTTAGAAGATTTTACTGACAATTTGGAG
  CTAGATGAAGAAGGAACAGGCGGGTTCACGGCTAACGCAATCGTTCAAAGAGACAGAGTGGATGAAGAGG
  CCTTGAATTTCTCCTATGAGGATGACTTTGACAATGATGTGGATGCTTTACTAGAAGAAGGTCTCTGTGC
  TCCCAAGAAGAGGCGAATGGAGGAAAAATATGGCGGAGACAGTGATCATCCGTCTGATGGAGAGACAAGT
  GTGCAGCCAATGATGACCAAGATTAAAACAGTGCTCAAAAGTCGTGGCCGTCCACCTACAGAGCCATTGC
  CTGATGGATGGATAATGACTTTTCATAACTCTGGAGTCCCTGTATACCTGCACAGAGAGTCTCGAGTGGT
  CACCTGGTCCAGACCCTACTTCTTGGGGACGGGAAGCATACGGAAACATGATCCTCCTCTAAGCAGCATC
  CCTTGTCTGCATTATAAGAAAATGAAGGACAACGAGGAACGAGAGCAAAGTTGTGATCTTGCCCCCAGTG
  GAGAAGTGTCACCTGTCAAGCCCCTGGGTCGCTCTGCAGAGCTGGATTTCCCTCTGGAGGAGCCTGACTC
  CTTGGGTGGAGACTCAGGGTCTCTGGATGAGAAGGACCCCCTGGGTGCTGAGGCAGCTGCTGGAGCCCTG
  GGACAAGTGAGGGCTAAAGTTGAGGTGTGCAAAGATGAGTCAGTTGATCTGGAGGAATTTCGTAATTACC
  TGGAGAAGCGATTTGACTTTGAGCAAGTAACTGTGAAAAAATTCAGGACTTGGGCTGAACGTCGTCAATT
  CAACCGTGAGATGAAGCGGAAACAGGCAGAGTCAGAGAGGCCCATCCTGCCAGCCAACCAGAAGCTGATC
  ACTCTATCTGTGCAAGATGCACCCACAAAAAAAGAGTTTGTCATCAATCCCAATGGGAAGTCTGAGGTTT
  GCATCCTGCACGAATACATGCAGCGTGTCCTCAAGGTCCGCCCTGTTTATAATTTCTTTGAATGTGAGAA
  TCCAAGTGAGCCTTTTGGTGCCTCCGTGACCATTGATGGTGTGACTTACGGATCTGGAACTGCAAGCAGC
  AAAAAACTTGCGAAGAATAAAGCTGCCCGAGCTACACTGGAAATTCTCATCCCTGACTTTGTTAAACAGA
  CCTCTGAGGAGAAGCCTAAAGACAGTGAGGAACTGGAGTATTTTAACCACATCAGTATTGAGGATTCGCG
  AGTCTACGAGCTGACAAGCAAGGCTGGGCTGTTGTCTCCATATCAGATCCTCCATGAGTGCCTTAAAAGA
  AACCATGGCATGGGTGACACATCCATCAAGTTTGAAGTGGTTCCTGGGAAAAACCAGAAGAGCGAATATG
  TTATGGCATGCGGCAAACACACAGTGCGCGGGTGGTGTAAGAATAAACGAGTTGGGAAACAATTAGCATC
  TCAGAAAATCCTTCAGCTGTTGCACCCCCATGTCAAGAACTGGGGGTCCTTGCTGCGCATGTATGGTCGT
  GAGAGCAGCAAAATGGTCAAGCAGGAGACCTCTGACAAGAGTGTGATAGAGCTACAACAGTATGCCAAGA
  AGAACAGGCCCAACCTTCACATCCTGAGCAAGCTACAAGAAGAGATGAAGAGGCTGGCTGCAGAGCGGGA
  GGAGACTCGGAAGAAACCCAAGATGTCAATTGTAGCATCTGCCCAGCCTGGTGGTGAGCCCTTGTGCACA
  GTGGATGTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR202911 representing NM_001105865
  Red=Cloning site Green=Tags(s)
  
  METYESPSPLPHEPTGEAMMENRACPFQVLPHEQSPPPPLQTSSDAEVMDVGSGGDGQSEPPADDPFNFY
  GASLLSKGSFSKGRLLIDPNCSGHSPRTARHAPAVRKFSPDLKLLKDVKISVSFTESCRSKDRKVLYTGV
  ERSTRPECSQLLSPVCGDVHACPFGGSVGNGVGLGGESTDKKDEENELDQEKRVEYAVLDELEDFTDNLE
  LDEEGTGGFTANAIVQRDRVDEEALNFSYEDDFDNDVDALLEEGLCAPKKRRMEEKYGGDSDHPSDGETS
  VQPMMTKIKTVLKSRGRPPTEPLPDGWIMTFHNSGVPVYLHRESRVVTWSRPYFLGTGSIRKHDPPLSSI
  PCLHYKKMKDNEEREQSCDLAPSGEVSPVKPLGRSAELDFPLEEPDSLGGDSGSLDEKDPLGAEAAAGAL
  GQVRAKVEVCKDESVDLEEFRNYLEKRFDFEQVTVKKFRTWAERRQFNREMKRKQAESERPILPANQKLI
  TLSVQDAPTKKEFVINPNGKSEVCILHEYMQRVLKVRPVYNFFECENPSEPFGASVTIDGVTYGSGTASS
  KKLAKNKAARATLEILIPDFVKQTSEEKPKDSEELEYFNHISIEDSRVYELTSKAGLLSPYQILHECLKR
  NHGMGDTSIKFEVVPGKNQKSEYVMACGKHTVRGWCKNKRVGKQLASQKILQLLHPHVKNWGSLLRMYGR
  ESSKMVKQETSDKSVIELQQYAKKNRPNLHILSKLQEEMKRLAAEREETRKKPKMSIVASAQPGGEPLCT
  VDV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001105865
• ORF Size: 2319 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001105865.1, NP_001099335.1
• RefSeq Size: 4538 bp
• RefSeq ORF: 2322 bp
• Locus ID: 287954
• Cytogenetics: 11q23
• MW: 86.4 kDa