Gdnf (NM_019139) Rat Tagged ORF Clone
CAT#: RR202605
- TrueORF®
Gdnf (Myc-DDK-tagged ORF) - Rat glial cell derived neurotrophic factor (Gdnf), (10 ug)
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_019139" in other vectors (3)
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Gdnf |
Synonyms | gndf |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR202605 representing NM_019139
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGTTATGGGATGTCGTGGCTGTCTGCCTGGTGTTGCTCCACACCGCGTCTGCCTTCCCGCTGCCCG CCGGTAAGAGGCTTCTCGAAGCGCCCGCCGAAGACCACTCCCTCGGCCACCGCCGCGTGCCCTTCGCGCT GACCAGTGACTCCAATATGCCCGAAGATTATCCTGACCAGTTTGATGACGTCATGGATTTTATTCAAGCC ACCATCAAAAGACTGAAAAGGTCACCAGATAAACAAGCGGCGGCACTTCCTCGAAGAGAGAGGAACCGGC AAGCTGCAGCTGCCAGCCCAGAGAATTCCAGAGGGAAAGGTCGCAGAGGCCAGAGGGGCAAAAATCGGGG GTGCGTCTTAACTGCAATACACTTAAATGTCACTGACTTGGGTTTGGGCTACGAAACCAAGGAGGAACTG ATCTTTCGATATTGTAGCGGTTCCTGTGAAGCGGCCGAGACAATGTACGACAAAATACTAAAAAATCTGT CTCGAAGTAGAAGGCTAACAAGTGACAAGGTAGGCCAGGCATGTTGCAGGCCGGTCGCCTTCGACGACGA CCTGTCGTTTTTAGACGACAGCCTGGTTTACCATATCCTAAGAAAGCATTCCGCTAAACGGTGTGGATGT ATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR202605 representing NM_019139
Red=Cloning site Green=Tags(s) MKLWDVVAVCLVLLHTASAFPLPAGKRLLEAPAEDHSLGHRRVPFALTSDSNMPEDYPDQFDDVMDFIQA TIKRLKRSPDKQAAALPRRERNRQAAAASPENSRGKGRRGQRGKNRGCVLTAIHLNVTDLGLGYETKEEL IFRYCSGSCEAAETMYDKILKNLSRSRRLTSDKVGQACCRPVAFDDDLSFLDDSLVYHILRKHSAKRCGC I TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_019139 |
ORF Size | 633 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_019139.1, NP_062012.1 |
RefSeq Size | 700 bp |
RefSeq ORF | 636 bp |
Locus ID | 25453 |
UniProt ID | Q07731 |
Cytogenetics | 2q16 |
MW | 23.6 kDa |
Gene Summary | neurotrophic factor specific for midbrain dopamine neurons; binds to GDNFR-alpha and mediates activation of the Ret protein tyrosine kinase receptor [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN202605 | Gdnf (untagged ORF) - Rat glial cell derived neurotrophic factor (Gdnf), (10 ug) |
USD 480.00 |
|
RR202605L3 | Lenti ORF clone of Gdnf (Myc-DDK-tagged ORF) - Rat glial cell derived neurotrophic factor (Gdnf), (10 ug) |
USD 780.00 |
|
RR202605L4 | Lenti ORF clone of Gdnf (mGFP-tagged ORF) - Rat glial cell derived neurotrophic factor (Gdnf), (10 ug) |
USD 780.00 |
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