Dhx9 (NM_001107184) Rat Tagged ORF Clone

CAT#: RR202048

Dhx9 (Myc-DDK-tagged ORF) - Rat DEAH (Asp-Glu-Ala-His) box polypeptide 9 (Dhx9), (10 ug)

Product Images

Circular map for RR202048

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dhx9
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR202048 representing NM_001107184
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTGATATTAAAAATTTTCTGTATGCCTGGTGTGGCAAAAGGAAGATGACTCCAGCCTATGAAATTA
  GAGCAGTAGGAAACAAAAACAGACAGAAATTCATGTGTGAGGTTCGAGTAGAAGGGTTTAATTATGCTGG
  TATGGGCAATTCCACCAATAAGAAAGATGCACAGAGCAATGCTGCCAGAGACTTCGTTAACTATTTGGTT
  CGAATCAATGAAGTCAAGAGTGAAGAAGTTCCTGCTGTTGGGATTGTACCACCCCCACCCATACTTAGTG
  ACACATCTGATAGCACAGCAAGTACTGGAGAAGGCTTACCACCACCCATGGGAGGACCACTTCCTCCTCA
  TCTGGCTCTCAAAGCAGCAGAAAATAATTCTGGAGTTGAGTCTTCATCTGGCTATGGTTCTCCTGGACCC
  ACCTGGGACCGAGGAGCCAACTTAAAGGATTACTACTCAAGAAAGGAAGAGCAAGAGGTGCAAGCGACTC
  TAGAGTCAGAAGAAGTGGATTTAAATGCTGGGCTTCATGGAAACTGGACTTTGGAAAATGCTAAGGCTCG
  TCTGAACCAGTATTTCCAAAAAGAAAAAATCCAAGGAGAATATAAGTACACCCAAGTGGGTCCCGACCAC
  AACAGGAGCTTTATTGCAGAAATGACCATTTATATCAAGCAGTTGGGCAGAAGGATTTTTGCACGTGAAC
  ATGGATCAAATAAGAAATTAGCAGCACAGTCATGTGCCCTCTCACTAGTCCGACAACTCTATCATCTTGG
  AGTGATTGAAGCTTACTCTGGTCTTACAAAGAAGAAAGAAGGAGAGAGAGTGGAGCCTTACAAAGTTTTC
  CTTTCCCCAGATTTGGAGCTTCAGTTGCAAAATGTGGTTCAAGAGCTAGATCTGGAAATTGTTCCCCCAC
  CTGTTGATCCTTCTATGCCAGTTATACTCAACATTGGGAAATTGGCTCATTTTGAACCATCTCAGAGGCA
  AAATGCAGTGGGAGTGGTTCCTTGGTCACCCCCACAGTCCAACTGGAATCCTTGGACAAGTAGCAACATT
  GATGAGGGGCCCCTTGCTTATGCTTCTACAGAACAAATAAGCATGGACCTTAAGAATGAATTAGCATACC
  AGATGGAACAGGATCATAATTTACAGAGTGTGTTACAAGAGAGAGAGTTGCTTCCTGTGAAGAAATTTGA
  AGCTGAAATTCTGGAAGCCATTAGTCAAAATTCAGTTGTGATTATCCGAGGGGCTACTGGTTGTGGTAAA
  ACCACGCAAGTTCCACAATACATTTTGGATGACTTCATCCAGAATGACAGAGCAGCTGAGTGCAATATTG
  TTGTGACTCAGCCCAGAAGGATCAGTGCAGTTGCGGTGGCTGAGCGAGTTGCTTATGAGAGAGGAGAAGA
  ACCTGGAAAAAGCTGTGGCTACAGTGTCCGGTTTGAGTCTATACTTCCACGCCCTCATGCCAGTATAATG
  TTTTGTACTGTAGGTGTGCTACTAAGAAAACTAGAAGCAGGCATTCGAGGAATCAGTCATGTTATTGTAG
  ATGAAATTCATGAAAGAGATATTAATACTGACTTCCTCTTAGTAGTACTGCGTGACGTGGTTCTGGCTTA
  TCCTGAAGTTCGTATTGTTCTTATGTCTGCAACTATTGATACCACCATGTTCTGTGAATATTTCTTCAAT
  TGCCCCATCATTGAAGTTTATGGAAGGACTTTCCCAGTTCAAGAATATTTTCTGGAAGATTGCATTCAGA
  TGACCCAATTCATTCCTCCTCCAAAGGACAAGAAAAAGAAGGATAAAGAGGATGATGGTGGTGAAGATGA
  TGACGCAAATTGCAATCTCATATGTGGTGACGAATATGGACCAGAAACAAAGTTGAGCATGTCTCAGTTG
  AATGAAAAGGAAACTCCCTTTGAACTTATTGAAGCACTCCTTAAATACATTGAAACCCTTAATGTTCCTG
  GAGCTGTGTTGGTATTTTTACCTGGCTGGAACTTGATTTATACTATGCAGAAGCATTTAGAAAATAACTC
  ACATTTCGGAAGTCACCGGTATCAGATTCTACCTCTCCATTCTCAGATTCCTCGGGAGGAACAGCGCAAA
  GTGTTTGATCCAGTACCAGATGGAGTAACCAAGGTTATTTTGTCCACAAACATTGCTGAAACAAGCATTA
  CCATAAATGATGTTGTTTATGTCATTGACTCCTGCAAACAAAAAGTGAAACTCTTTACTGCTCACAACAA
  TATGACCAACTATGCTACTGTATGGGCATCAAAAACAAACCTTGAGCAAAGGAAAGGGCGAGCTGGCCGT
  GTACGACCTGGGTTCTGTTTCCACCTTTGTAGTCGGGCTCGTTTTGACAGACTGGAGACCCACATGACAC
  CAGAGATGTTTAGAACACCATTGCATGAAATTGCTCTGAGCATAAAACTTCTGCGCCTAGGAGGAATTGG
  CCAATTCCTTGCAAAGGCAATTGAACCTCCACCTTTGGATGCAGTAATTGAAGCAGAGCATACTCTTAGA
  GAACTTGATGCTTTAGATGCCAATGATGAGTTGACTCCTCTTGGACGAATCCTGGCTAAACTCCCCATTG
  AGCCTCGTTTTGGAAAAATGATGATAATGGGGTGTATTTTCTATGTGGGAGACGCTGTCTGTACCATCTC
  TGCTGCTACCTGCTTTCCAGAGCCCTTTATCAGTGAAGGGAAGCGCTTGGGGTACATACATCGGAATTTT
  GCTGGAAACAGATTTTCTGATCATGTGGCTCTTTTATCAGTATTCCAAGCCTGGGATGATGCTAGGATGA
  GTGGAGAAGAAGCAGAAATACGGTTTTGTGAGCAGAAGAGACTAAATATGGCTACACTAAGAATGACTTG
  GGAAGCCAAAGTACAGCTTAAAGAGATACTGATTAACTCTGGCTTTCCAGAAGATTGTTTATTGACACAA
  GTGTTTACTAACACTGGACCAGATAATAATTTGGATGTTGTTATCTCTTTGCTGGCTTTTGGTGTGTACC
  CCAATGTATGCTATCATAAAGAGAAGAGAAAGATTCTTACCACTGAAGGTCGTAATGCACTTATCCACAA
  GTCTTCTGTGAACTGTCCTTTTAGCAGCCAAGACATGAAGTACCCATCTCCTTTCTTTGTTTTTGGTGAA
  AAGATTCGAACCCGAGCCATCTCTGCTAAAGGCATGACCTTAGTGACCCCTCTACAATTACTTCTCTTTG
  CCTCCAAGAAAGTCCAGTCTGATGGTCAGATCGTGTTTATAGATGACTGGATCAGACTGCAAATATCACA
  TGAAGCTGCAGCATGCATCACAGCCCTCCGGGCAGCCATGGAAGCTCTGGTTGTTGAAGTATCCAAACAA
  CCAAACATTATCAGCCAGTTGGACCCTGTCAATGAGCATATGCTAAACACCATCCGTCAGATTTCTAGGC
  CCTCAGCTGCTGGTATCAACCTTATGATTGGGAGTGTAAGGTATGGCGATGGTCCGAGGCCTCCCAAGAT
  GGCCCGATACGATAATGGAATA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR202048 representing NM_001107184
  Red=Cloning site Green=Tags(s)
  
  MGDIKNFLYAWCGKRKMTPAYEIRAVGNKNRQKFMCEVRVEGFNYAGMGNSTNKKDAQSNAARDFVNYLV
  RINEVKSEEVPAVGIVPPPPILSDTSDSTASTGEGLPPPMGGPLPPHLALKAAENNSGVESSSGYGSPGP
  TWDRGANLKDYYSRKEEQEVQATLESEEVDLNAGLHGNWTLENAKARLNQYFQKEKIQGEYKYTQVGPDH
  NRSFIAEMTIYIKQLGRRIFAREHGSNKKLAAQSCALSLVRQLYHLGVIEAYSGLTKKKEGERVEPYKVF
  LSPDLELQLQNVVQELDLEIVPPPVDPSMPVILNIGKLAHFEPSQRQNAVGVVPWSPPQSNWNPWTSSNI
  DEGPLAYASTEQISMDLKNELAYQMEQDHNLQSVLQERELLPVKKFEAEILEAISQNSVVIIRGATGCGK
  TTQVPQYILDDFIQNDRAAECNIVVTQPRRISAVAVAERVAYERGEEPGKSCGYSVRFESILPRPHASIM
  FCTVGVLLRKLEAGIRGISHVIVDEIHERDINTDFLLVVLRDVVLAYPEVRIVLMSATIDTTMFCEYFFN
  CPIIEVYGRTFPVQEYFLEDCIQMTQFIPPPKDKKKKDKEDDGGEDDDANCNLICGDEYGPETKLSMSQL
  NEKETPFELIEALLKYIETLNVPGAVLVFLPGWNLIYTMQKHLENNSHFGSHRYQILPLHSQIPREEQRK
  VFDPVPDGVTKVILSTNIAETSITINDVVYVIDSCKQKVKLFTAHNNMTNYATVWASKTNLEQRKGRAGR
  VRPGFCFHLCSRARFDRLETHMTPEMFRTPLHEIALSIKLLRLGGIGQFLAKAIEPPPLDAVIEAEHTLR
  ELDALDANDELTPLGRILAKLPIEPRFGKMMIMGCIFYVGDAVCTISAATCFPEPFISEGKRLGYIHRNF
  AGNRFSDHVALLSVFQAWDDARMSGEEAEIRFCEQKRLNMATLRMTWEAKVQLKEILINSGFPEDCLLTQ
  VFTNTGPDNNLDVVISLLAFGVYPNVCYHKEKRKILTTEGRNALIHKSSVNCPFSSQDMKYPSPFFVFGE
  KIRTRAISAKGMTLVTPLQLLLFASKKVQSDGQIVFIDDWIRLQISHEAAACITALRAAMEALVVEVSKQ
  PNIISQLDPVNEHMLNTIRQISRPSAAGINLMIGSVRYGDGPRPPKMARYDNGI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001107184
• ORF Size: 3522 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001107184.1, NP_001100654.1
• RefSeq Size: 4205 bp
• RefSeq ORF: 3525 bp
• Locus ID: 304859
• Cytogenetics: 13q21
• MW: 131.7 kDa