Rasgrp3 (NM_001108009) Rat Tagged ORF Clone
CAT#: RR201858
- TrueORF®
Rasgrp3 (Myc-DDK-tagged ORF) - Rat RAS guanyl releasing protein 3 (calcium and DAG-regulated) (Rasgrp3), (10 ug)
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_001108009" in other vectors (3)
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Rasgrp3 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR201858 representing NM_001108009
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGTCCAACGGCCTCGGGAAAGCGGCGACACTAGATGAACTTCTCAGCACATGCATTGAGATGTTTG ATGACAATGGAGAACTGAATGACAGCTACTTGCCCAGAATCATGCTACTGATGCATCGATGGTATTTATC CTCCACTGAACTGGCAGGAAAACTCCTATGCATGTATCGGAATGCCAGCGGAGAAAGTTGTGATGAGTTC CGACTGAAGATCTGCTATTTCATGAGGTACTGGATTACAAAATTTCCTGCAGAGTTTAATTTGGATCTTG GTTTGATTCGCATGACTGAGGAGTTCCGGGAAGTAGCTAGTCAACTAGGATATGAGAAACACAGCAACCT CATGGACATATCCAGCATTCCTTCCTATGACTGGATGAGGAGAGTCACGCAGAGGAAAAAAGTATCAAAA AGGGGAAAGGCCTGTCTCTTATTTGACCATCTGGAGCCCATTGAATTGGCTGAGCACCTCACTTTTCTGG AGCATAAATCTTTTAGAAGGATCTCGTTCACAGATTATCAGAGCTACGTCATTCATGGCTGCCTGGAGAA TAACCCGACATTGGAGAGATCTATTGCCTTATTTAATGGAATCTCTAAGTGGGTCCAATTGATGGTTCTT AGCAAACCAAGTGCTCAACAAAGGGCGGAAGTCATCACAAAGTTCATCAATGTAGCACAGAAACTCCTTC AACTAAAAAATTTTAACACCTTGATGGCTGTGGTTGGTGGCCTTAGTCACAGCTCCATTTCCCGACTCAA AGATACCCATTCTCACCTCTCTTCTGAAGTTACAAAGAACTGGAATGAGATGACAGAGTTGGTCTCCTCC AACGGCAATTACTGCAATTACCGCAAGGCCTTTGCTGACTGTGATGGCTTCAAAATCCCCATCCTTGGCG TGCACTTGAAAGACTTGATAGCAGTCCATGTCATTTTCCCAGACTGGATGGAAGAGAACAAAGTGAACGT TGTGAAAATGCACCAGCTTTCTGTTACCCTGAGTGAACTGGTCTCCCTGCAGAATGCCTCTCACCACTTG GAGCCTAACATGGATTTGATCAACCTCCTCACTCTCTCTCTGGACCTCTACCACACAGAAGACGATATTT ATAAACTATCACTGGTGCTGGAGCCTAGGAATTCCAAATCACAGCCAACTTCCCCCACAACACCTAATAA GCCTGTGGTACCCCTGGAGTGGGCTTCAGAAGTGGTACCAAAGCCAGACCCCACAATCATCAACAAACAC ATAAGGAAGTTGGTTGAGCTCTGGGGCATAATCAAACAAGGCTACAAATGCAAAGACTGTGGAGCCAACT GTCACAAACAGTGCAAAGACCTCTTGGTTCTGGCCTGTAGGAGACTTGCCAGGGCACCCTCCTTGAGCAG CAACCCTGAGTCACTGCCTGGAAGCCCAGCCTTGCCCCCAGTGCAGGATGAGGTGTTTGAGTTTCCTGGC GTCACTGCTAGACACCGAGACCTGGACAGCAGAGACATCACGCTGGTTACAGGATCTTCCCGTAAGATCT CCGTGAGAGTGCAGAGAGCCACCACTAGTCAAGCCACGCAAACCGAACCTGTGTGGTCAGAGGCTGTCTG GGGAGACTCTGGGTCCCATACCTTCCCCAAAATGAAATCCAAGCTCCATGACAAAGCAGCAAAGGACAAA GGCTTTGCCAAGTGGGAAAATGAAAAGCCCACGCTACACGCTGGTGTGGATGTTGTAGATCGAGGCACTG CATTCGAACCTGACCAGGACGATGAGCAAGATGAAGCCCAACAGGGTGTCGAGGATGGCCATTCGAACCT GACCAGGACGATGAGCAAGATGAAGCCCAACAGGGTGTCGAGGATGGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR201858 representing NM_001108009
Red=Cloning site Green=Tags(s) MGSNGLGKAATLDELLSTCIEMFDDNGELNDSYLPRIMLLMHRWYLSSTELAGKLLCMYRNASGESCDEF RLKICYFMRYWITKFPAEFNLDLGLIRMTEEFREVASQLGYEKHSNLMDISSIPSYDWMRRVTQRKKVSK RGKACLLFDHLEPIELAEHLTFLEHKSFRRISFTDYQSYVIHGCLENNPTLERSIALFNGISKWVQLMVL SKPSAQQRAEVITKFINVAQKLLQLKNFNTLMAVVGGLSHSSISRLKDTHSHLSSEVTKNWNEMTELVSS NGNYCNYRKAFADCDGFKIPILGVHLKDLIAVHVIFPDWMEENKVNVVKMHQLSVTLSELVSLQNASHHL EPNMDLINLLTLSLDLYHTEDDIYKLSLVLEPRNSKSQPTSPTTPNKPVVPLEWASEVVPKPDPTIINKH IRKLVELWGIIKQGYKCKDCGANCHKQCKDLLVLACRRLARAPSLSSNPESLPGSPALPPVQDEVFEFPG VTARHRDLDSRDITLVTGSSRKISVRVQRATTSQATQTEPVWSEAVWGDSGSHTFPKMKSKLHDKAAKDK GFAKWENEKPTLHAGVDVVDRGTAFEPDQDDEQDEAQQGVEDGHSNLTRTMSKMKPNRVSRM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001108009 |
ORF Size | 1809 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq Size | 4293 bp |
RefSeq ORF | 1812 bp |
Locus ID | 313874 |
Cytogenetics | 6q12-q13 |
MW | 68.3 kDa |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN201858 | Rasgrp3 (untagged ORF) - Rat RAS guanyl releasing protein 3 (calcium and DAG-regulated) (Rasgrp3), (10 ug) |
USD 618.00 |
|
RR201858L3 | Lenti ORF clone of Rasgrp3 (Myc-DDK-tagged ORF) - Rat RAS guanyl releasing protein 3 (calcium and DAG-regulated) (Rasgrp3), (10 ug) |
USD 917.00 |
|
RR201858L4 | Lenti ORF clone of Rasgrp3 (mGFP-tagged ORF) - Rat RAS guanyl releasing protein 3 (calcium and DAG-regulated) (Rasgrp3), (10 ug) |
USD 917.00 |
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