Polr3k (NM_001014259) Rat Tagged ORF Clone
CAT#: RR201422
- TrueORF®
Polr3k (Myc-DDK-tagged ORF) - Rat polymerase (RNA) III (DNA directed) polypeptide K, 12.3 kDa (Polr3k), (10 ug)
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_001014259" in other vectors (3)
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Polr3k |
Synonyms | MGC109430 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR201422 representing NM_001014259
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCTGTTCTGCCCGGGCTGCGGGAACGGCCTCATTGTGGAGGAGGGGCAGCGCTGTCACCGCTTCG CCTGCAACACGTGCCCGTACGTCCATAACATCACCCGCAAGGTAACAAACCGAAAGTATCCAAAGCTAAA AGAAGTGGATGATGTGCTTGGTGGAGCAGCTGCCTGGGAGAATGTGGACTCTACTGCAGAGCCATGTCCA AAATGTGAGCATCCTCGTGCCTACTTCATGCAGCTTCAGACCCGTTCTGCGGACGAGCCAATGACCACCT TCTATAAGTGCTGCAATGCTCAGTGTGGACATCGCTGGAGGGATTCTATAAGTGCTGCAATGCTCAGTGT GGACATCGCTGGAGGGAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR201422 representing NM_001014259
Red=Cloning site Green=Tags(s) MLLFCPGCGNGLIVEEGQRCHRFACNTCPYVHNITRKVTNRKYPKLKEVDDVLGGAAAWENVDSTAEPCP KCEHPRAYFMQLQTRSADEPMTTFYKCCNAQCGHRWRDSISAAMLSVDIAGG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001014259 |
ORF Size | 324 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq Size | 995 bp |
RefSeq ORF | 327 bp |
Locus ID | 366277 |
Cytogenetics | 3q43 |
MW | 12.3 kDa |
Gene Summary | estrogen responsive protein; may be regulated by 17beta-estradiol [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN201422 | Polr3k (untagged ORF) - Rat polymerase (RNA) III (DNA directed) polypeptide K, 12.3 kDa (Polr3k), (10 ug) |
USD 165.00 |
|
RR201422L3 | Lenti ORF clone of Polr3k (Myc-DDK-tagged ORF) - Rat polymerase (RNA) III (DNA directed) polypeptide K, 12.3 kDa (Polr3k), (10 ug) |
USD 465.00 |
|
RR201422L4 | Lenti ORF clone of Polr3k (mGFP-tagged ORF) - Rat polymerase (RNA) III (DNA directed) polypeptide K, 12.3 kDa (Polr3k), (10 ug) |
USD 465.00 |
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